• Title/Summary/Keyword: Resistant length

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Comparison of Stress Response in Diallel Crossed Korean Domestic Chicken Breeds (토종 종계를 이용한 이면 교배조합 계통 간 스트레스 반응정도 비교 분석)

  • Cho, Eun Jung;Park, Ji Ae;Choi, Eun Sik;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.43 no.2
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    • pp.77-88
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    • 2016
  • To establish a new synthetic Korean meat chicken breed, we tested $5{\times}5$ diallel cross mating experiment with domestic chicken breeds. Comparing stress responses among diallel crossed chicken breeds, we analyzed telomere length, DNA damage and expressions of heat shock protein genes (HSPs) as the markers of the stress response. The telomere length was measured by quantitative fluorescence in situ hybridization on the nuclei of lymphocytes. The expression levels of HSP-70, $HSP-90{\alpha}$ and $HSP-90{\beta}$ genes were analyzed by quantitative real-time polymerase chain reaction in lymphocytes. The DNA damage rate of lymphocytes was quantified by the comet assay known as the single cell gel electrophoresis. In results, there were significant differences in the values of the stress markers such as telomere length, HSPs and DNA damage rate, and also were significant differences in viabilities and body weights among the $5{\times}5$ diallel crossed chicken breeds. The telomere shortening rate, expression values of HSPs and DNA damage rate were significant low in W and Y crossed chickens compare to the others, but GG pure breed showed the highest values in the 25 crossed chickens. Estimating correlation coefficient, the survival rate positively correlated to telomere length, but negatively correlated to the expression levels of HSP-70, $HSP-90{\alpha}$, $HSP-90{\beta}$ genes and to the value of % DNA in tail as DNA damage rate. The expression levels of HSP-70, $HSP-90{\alpha}$ and $HSP-90{\beta}$ genes of dead chickens had significantly higher than those of survival chickens. According to the results on the stress marker analysis, it would be considered that the crossed breeds had more stress resistant than the pure breeds, and the crossed chickens with a light strain such as W or Y were relatively resistant to stress, but the crossed chickens with a heavy strain such as G, H, F were susceptible to stress.

Marker Assisted Selection of Brown Planthopper Resistance and Development of Multi-Resistance to Insect and Diseases in Rice (Oryza sativa L.) (DNA 마커를 이용한 벼멸구 저항성 선발 및 복합내병충성 벼 계통 육성)

  • Lee, Jong-Hee;Yeo, Un-Sang;Cho, Jun-Hyun;Lee, Ji-Yoon;Song, You-Chun;Shin, Mun-Sik;Kang, Hang-Won;Sohn, Jae-Keun
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.413-421
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    • 2011
  • The main objective of this study was to develop the multi-resistance lines to insects(brown planthopper; BPH, rice green leafhopper; GRH) and disease(blast; BL, bacterial blight; BB and rice stripe virus disease;RSV) with good grain quality and plant type by combining conventional breeding and marker assisted selection(MAS) and to eliminate the linkage drag effects between Bph1 gene and culm length, we conducted MAS of Bph1 gene in advanced backcross and double cross progenies. 'Nampyeong', 'Junam' and 'Milyang220' were used as the parent in this study. 'Milyang220' was used as the donor of brown planthopper resistance gene Bph1 with tall culm length. Two backcross progenies were developed using two recipients 'Nampyeong' carrying GRH resistance gene Grh3(t) with good grain appearance and 'Junam' harboring bacterial blight resistance gene Xa3 with short culm length. Two $BC_1$ generations were resulted from the backcrossing of the $F_1$ plants with recurrent parents 'Nampyeong' and 'Junam'. The second rounds of backcrossing($BC_2$) were derived from the cross of selected resistant $BC_1F_1$ plants based on heterozygous genotype of RM28493 linked to Bph1 gene. The double crossed population was constructed from the cross of between each heterozygous $BC_2F_1$ plants at RM28493 locus of '$Nampyeong^*3$ / Milyang220' and '$Junam^*3$ / Milyang220'., The homozygous alleles in Bph1 gene were selected using co-dominant DNA marker RM28493 in double crossed population. Eighty-five lines with multi-resistance to BL, BB, RSV, GRH and BPH were selected by bio-assay and MAS in generation of double crossing. The culm length, head rice ratio and yield of the selected multi resistance lines was ranged from 71 to 88 cm, from 51 to 93%, from 449 to 629 kg/10a. respectively. We can select a promising multi resistance line similar with 'Nampyeong' of major agronomic traits such as culm legnth, head rice ratio and yield. It was designated as Milyang265. Finally this study was developed the multi resistant varieties against to insects and diseases with the good grain quality 'Milyang265' by the advanced backcross and double cross combining MAS and it can be used as genetic resources of multi-resistance to insect and diseases in rice breeding programs.

Monitoring of Bacteria using PCR Method and Inactivation with Ozone and UV (PCR법에 의한 박테리아의 동정 및 오존과 UV에 의한 제거 연구)

  • Oh, Byung-Soo;Ju, Seul;Kim, Kyoung-Suk;Kang, Tae-Hee;Lee, Ji-Young;Lee, Hye-Young;Kang, Joon-Wun
    • Journal of Korean Society on Water Environment
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    • v.20 no.2
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    • pp.170-175
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    • 2004
  • The purpose of this study was to monitor bacteria present in raw water and to investigate the effect of ozone, UV and combined ozone/UV processes for inactivating bacteria. Both polymerase chain reaction (PCR)-fragment length polymorphic analysis (PRA) and PCR-sequence analysis (PSA) were applied for the simultaneous analysis of numerous bacteria species present in each tested water, such as drinking water (DRW), drinking water source (DRWS) and sewage effluent water (SEW). According to the result, the number of detected bacteria species was zero in DRW, 58 in DRWS and 13 in SEW. After treatment of the each process, the ozone/UV process was the most successful for inactivating almost all bacteria. However, it was found that Flavobacterium sp., Pseudomonas sp. and Beta proteobacterium sp. had strong resistant to all tested processes, requiring further detailed study.

An Experimental Study on the Anchorage Capacity by Diameter and Anchor Type of Re-bar (콘크리트 구조체 매입 철근의 직경 및 유형별 앵커력 측정실험 연구)

  • Cho, Seong-Yeol;Son, Ki-Sang
    • Journal of the Korean Society of Safety
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    • v.29 no.5
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    • pp.67-73
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    • 2014
  • Construction equipment such as tower crane should be installed in a field without appropriate anchorage to cause a collapse of crane. The anchorage capacity can be varied with Anchor length, concrete strength, anchor diameter, hooked or non hooked these variables will be made and tested in the study. It is shown what anchorage capacity will be more effective case by case. Hooked and non-hooked rebar anchor concrete weight with dia 22mm rebar are shown with initial displacement at 170~220KN of hooked case and 200~210KN of non-hooked one which are linearly increased, without any ductility behavior with almost brittleness. Three(3) same test pieces are almost similarly behaviored without relation to hooked or non-hooked cases. It is found out that the bigger diameter of rebar becomes, the more resistant capacity could be made, but conversely ductility against sudden collapse similar to brittleness becomes the more insufficient. It is also found out that dia 16mm rebar could be more effectively applied to heavy support weight at construction sites.

Monitoring of wind effects on an instrumented low-rise building during severe tropical storm

  • Li, Q.S.;Hu, S.Y.
    • Wind and Structures
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    • v.20 no.3
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    • pp.469-488
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    • 2015
  • A full-scale instrumented low-rise building with gable roof was built at a coastal site with a high incidence of tropical cyclones for monitoring of wind effects on the building during windstorms. This paper presents the field measurements of the wind velocity field around and the wind-induced pressures on the low-rise building during the passage of severe tropical storm Soudelor. Near-ground wind characteristics such as wind speed, wind direction, turbulence intensity, gust factor, turbulence integral length scale and wind velocity spectra were investigated. The wind-induced pressures on the roof of the building were analyzed and discussed. The results revealed that the eave and ridge edges on the roof were subjected to the most severe suction pressures under quartering winds. These suction pressures showed obvious non-Gaussian behavior. The measured results were compared with the provisions of ASCE 7-10 to assess the suitability of the code of practice for the wind-resistant design of low-rise buildings under tropical cyclones. The field study aims to provide useful information that can enhance our understanding of the extreme wind effects on low-rise buildings in an effort to reduce tropical cyclone wind damages to residential buildings.

Reproduction Strategies of Clonal Plants of Potentilla conferta in Uzbekistan and Mongol

  • Huh, Man-Kyu;Lee, Byeong-Ryong
    • Journal of Environmental Science International
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    • v.21 no.11
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    • pp.1297-1305
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    • 2012
  • Clonal plants combine sexual and clonal reproduction, which contribute differently to plant fitness. Reproductive analyses have highlighted the importance of clonal growth in shaping the spatial genetic structure in Potentilla conferta Bunge, a herbaceous rhizomatic clonal distributed in hot sand dunes. We investigated the reproduction system of P. conferta at two populations in Mongol and three natural populations in Uzbekistan. The measurements of 19 quantitative or qualitative morphological characters were taken on each of total individuals directly from their natural habitats. Some morphological characteristics between Mongolia and Uzbekistan populations showed a slight heterogeneity of variance. Especially, the length of internodes (LFI and LSI) and characteristics of root (LLR and NOR) were shown a significant difference between two countries (P<0.05). P. conferta of Uzbekistan has most ramets at short distance intervals 30~100 cm. In light conditions, P. conferta of Uzbekistan was significantly less resilience than P. conferta of Mogol. In drought conditions, although there was not shown significant difference, P. conferta in Uzbekistan was less resilience than that in Mogol. The core analysis indicates that P. conferta in Uzbekistan is the more resistant than that of Mongol and seems to do by sexual reproduction strategy during several strong environmental disadvantages such as drought events.

Studies for Physicochemical and In Vitro Digestibility Characteristics of Flour and Starch from Chickpea (Cicer arietinum L.)

  • Chung, Hyun-Jung
    • Preventive Nutrition and Food Science
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    • v.16 no.4
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    • pp.339-347
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    • 2011
  • Flour and isolated starch from chickpea (desi type, 328S-8) were evaluated for their in vitro digestibility and physicochemical properties. The protein content, total starch content and apparent amylose content of chickpea flour and isolated starch were 22.2% and 0.6%, 45.8% and 91.5%, and 11.7% and 35.4%, respectively. Chickpea starch granules had an oval to round shape with a smooth surface. The X-ray diffraction pattern of chickpea starch was of the C-type and relative crystallinity was 24.6%. Chickpea starch had only a single endothermic transition (13.3 J/g) in the DSC thermogram, whereas chickpea flour showed two separate endothermic transitions corresponding to starch gelatinization (5.1 J/g) and disruption of the amylose-lipid complex (0.7 J/g). The chickpea flour had a significantly lower pasting viscosity without breakdown due to low starch content and interference of other components. The chickpea starch exhibited significant high setback in the viscogram. The average branch chain length, proportion of short branch chain (DP 6~12), and long branch chains (DP${\geq}$37) of isolated chickpea starch were 20.1, 20.9% and 9.2%, respectively. The rapidly digestible starch (RDS), slowly digestible starch (SDS) and resistant starch (RS) contents of chickpea flour and starch were 9.9% and 21.5%, 28.7% and 57.7%, and 7.1% and 9.3%, respectively. The expected glycemic index (eGI) of chickpea flour (39.5), based on the hydrolysis index, was substantially lower than that of isolated chickpea starch (69.2).

Field monitoring of boundary layer wind characteristics in urban area

  • Li, Q.S.;Zhi, Lunhai;Hu, Fei
    • Wind and Structures
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    • v.12 no.6
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    • pp.553-574
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    • 2009
  • This paper presents statistical analysis results of wind speed and atmospheric turbulence data measured from more than 30 anemometers installed at 15 different height levels on 325 m high Beijing Meteorological Tower and is primarily intended to provide useful information on boundary layer wind characteristics for wind-resistant design of tall buildings and high-rise structures. Profiles of mean wind speed are presented based on the field measurements and are compared with empirical models' predictions. Relevant parameters of atmospheric boundary layer at urban terrain are determined from the measured wind speed profiles. Furthermore, wind velocity data in longitudinal, lateral and vertical directions, which were recorded from an ultrasonic anemometer during windstorms, are analyzed and discussed. Atmospheric turbulence information such as turbulence intensity, gust factor, turbulence integral length scale and power spectral densities of the three-dimensional fluctuating wind velocity are presented and used to evaluate the adequacy of existing theoretical and empirical models. The objective of this study is to investigate the profiles of mean wind speed and atmospheric turbulence characteristics over a typical urban area.

Evaluation of monotonic and cyclic behaviour of geotextile encased stone columns

  • Ardakani, Alireza;Gholampoor, Naeem;Bayat, Mahdi;Bayat, Mahmoud
    • Structural Engineering and Mechanics
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    • v.65 no.1
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    • pp.81-89
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    • 2018
  • Stone column installation is a convenient method for improvement of soft ground. In very soft clays, in order to increase the lateral confinement of the stone columns, encasing the columns with high stiffness and creep resistant geosynthetics has proved to be a successful solution. This paper presents the results of three dimensional finite element analyses for evaluating improvement in behaviour of ordinary stone columns (OSCs) installed in soft clay by geotextile encasement under monotonic and cyclic loading by a comprehensive parametric study. The parameters include length and stiffness of encasement, types of stone columns (floating and end bearing), frictional angle and elastic modulus of stone column's material and diameter of stone columns. The results indicate that increasing the stiffness of encasement clearly enhances cyclic behaviour of geotextile encased stone columns (GESCs) in terms of reduction in residual settlement. Performance of GESCs is less sensitive to internal friction angle and elasticity modulus of column's materials in comparison with OSCs. Also, encasing at the top portion of stone column up to triple the diameter of column is found to be adequate in improving its residual settlement and at all loading cycles, end bearing columns provide much higher resistance than floating columns.

Performance Investigation of Insulated Shallow Extension Silicon On Nothing (ISE-SON) MOSFET for Low Volatge Digital Applications

  • Kumari, Vandana;Saxena, Manoj;Gupta, R.S.;Gupta, Mridula
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.13 no.6
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    • pp.622-634
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    • 2013
  • The circuit level implementation of nanoscale Insulated Shallow Extension Silicon On Nothing (ISE-SON) MOSFET has been investigated and compared with the other conventional devices i.e. Insulated Shallow Extension (ISE) and Silicon On Nothing (SON) using the ATLAS 3D device simulator. It can be observed that ISE-SON based inverter shows better performance in terms of Voltage Transfer Characteristics, noise margin, switching current, inverter gain and propagation delay. The reliability issues of the various devices in terms of supply voltage, temperature and channel length variation has also been studied in the present work. Logic circuits (such as NAND and NOR gate) and ring oscillator are also implemented using different architectures to illustrate the capabilities of ISE-SON architecture for high speed logic circuits as compared to other devices. Results also illustrates that ISE-SON is much more temperature resistant than SON and ISE MOSFET. Hence, ISE-SON enables more aggressive device scaling for low-voltage applications.