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Monitoring of Bacteria using PCR Method and Inactivation with Ozone and UV  

Oh, Byung-Soo (Department of Environmental Engineering, Yonsei University)
Ju, Seul (Department of Environmental Engineering, Yonsei University)
Kim, Kyoung-Suk (Department of Environmental Engineering, Yonsei University)
Kang, Tae-Hee (Department of Environmental Engineering, Yonsei University)
Lee, Ji-Young (Department of Clinical pathology, Yonsei University)
Lee, Hye-Young (Department of Clinical pathology, Yonsei University)
Kang, Joon-Wun (Department of Environmental Engineering, Yonsei University)
Publication Information
Journal of Korean Society on Water Environment / v.20, no.2, 2004 , pp. 170-175 More about this Journal
Abstract
The purpose of this study was to monitor bacteria present in raw water and to investigate the effect of ozone, UV and combined ozone/UV processes for inactivating bacteria. Both polymerase chain reaction (PCR)-fragment length polymorphic analysis (PRA) and PCR-sequence analysis (PSA) were applied for the simultaneous analysis of numerous bacteria species present in each tested water, such as drinking water (DRW), drinking water source (DRWS) and sewage effluent water (SEW). According to the result, the number of detected bacteria species was zero in DRW, 58 in DRWS and 13 in SEW. After treatment of the each process, the ozone/UV process was the most successful for inactivating almost all bacteria. However, it was found that Flavobacterium sp., Pseudomonas sp. and Beta proteobacterium sp. had strong resistant to all tested processes, requiring further detailed study.
Keywords
Bacteria; PCR; Monitoring; Inactivation; Ozone; UV;
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