• 미생물학회지
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• 제6권1호
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• pp.1-5
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• 1968

This experiment was undertaken the purpose of investigating the difference of the content of free amino acids in the diseased and healthy leaves of the resistance and susceptible rice varieties, to blast by means of paper chromatography. The results obtained are summarized as follows. (1) In the healthy and diseased leaves, the former was founded nine to tell sorts of free amino acids and later was observed eleven. In the both of leaves, alanine and valine were contained in larger amount, and aspartic acid, glycine and arginine were found relatively less in general. (2) In the diseased leaves, the amino acids were increased in quantity, especially glutamine and aspartic acid were remarkable, and cystine were found only in the diseased ones. (3) It seems to that the health leaves in all varieties were observed the amino acid contents, in notably increase and decrease, but in the diseased ones were not changed in amount. Tatacho, susceptible variety was more contained the amount of glutamine and aspartic acid than Zenith, resistance one.

• 한국응용곤충학회지
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• 제14권1호
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• pp.13-21
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• 1975

1974년도 IRRI(국제미작연구소)에서 새로 입수된 48개 종품 및 게통을 공시하여 한국산 끝동매미충에 대한 저항성품종 및 게통을 선발하고 나아가 몇가지 저항성기작을 검토하기 위해 본 시험을 실하여 다음과 같은 결과를 얻었다. 1. 끝동매미충에 대한 유묘검정에서 48개 품종 또는 계통중 저항성반응(R)을 보인 것은 Balamawee, IR-26, IR-1529-525-2-2, IR-1539-1042-6, IR2061-214-2-3-5, IR2061-214-2-3-6, Jhingasail, Murungakayan 및 Vunam등 9개 품종 또는 계통이었고 중도저항성반응(MR)을 보인 것들로서는 Chianung-Shen-Yu-11, C62-1-230, C62-1-373, Dikwee, D204-1, Murungakayan-3, Palasithari-601, Peta-2802, RP6-516-34-1-8(TKM $6\times IR8$), RP6-590-10-1-11(TKM6$\times$IR8), RP9-4(IR8$\times$W1251)등 11개 품종 또는 계통이었다. 2. 저항성 및 중도저항성반응을 보인 것들을 공시하여 약충접종에 의한 저충성검정시험결과 저항성반응을 보인 것은 모두 $100\%$의 사충율을 보였으나 몇몇 품종 또는 계통에서 사충율이 좀 저하하였고 저조한 우화율을 나타내었다. 저항성반응을 보인 벼에서 2영기 약충이 살아 남을 수 있는 일수는 3-10일에 불과하였다. 즉, 유묘의 반응에서 저항성으로 나타난 벼들은 고도의 저충성이 있음을 보였다. 3. 저항성반응을 품종 또는 계통간에 차이가 없었을뿐더러 유묘의 저항성반응과도 아무런 상관이 없었다. 5. 이상의 결과를 종합해 볼 때 끝동매미충에 대한 벼의 저항성기작은 저항성 뼈는 고도의 항충성(Antibiosis)을 지니고 낮은 식이선호성(Non-feeding preference)을 나타내지만 감수성 벼는 반대로 항충성을 지니고 있지 않은데다가 높은 식이선호성을 나타내는데 있다고 보아진다.

• 한국작물학회지
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• 제7권1호
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• pp.123-127
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• 1969

소맥품종의 내한성 정도를 간이하게 판정할 수 있는 연구로서 내한성정도가 이미 알려진 3계급 9품종을 대상으로 하여 발아종자의 Amylase 활력의 강약과 품종의 내한성 정도와의 관계를 5시기 별로 시험했든바 그 성적을 요약하면 다음과 같다. 1. 각 시기별로는 Amylase 활력에 있어 유의차를 나타내지 않았으나 각품군간에는 고도의 유의차가 인정되었다. 2. 내한성에 강한 품종인 수원8005, 진광, 육성003는 다른 2군의 품종군보다 높은 Amylas의 활력을 보여주었고 내한성이 약한 품종인 적달마, 강도신력, 농촌004는 낮은 Amylase 활력을 나타냈으며 내한성이 중간정도인 농림006, 강도, 농림1002는 중간정도의 활력을 나타내었다. 3. 포장조건에서 내한성 정도가 같아보이는 품종간에서도 Amylase 활력을 강약이 인정되어 보다 세밀한 분류가 가능했다. 4. 시기별로 보면 제1～2일 째에서는 내한성 강, 중, 약 3품종군중 Amylase 활력의 차가 크지 않았으나 3일째에 3품종군간에 더욱 명백한 Amylase 활력의 차를 보여 주었고 제4일과 제5일에는 다시 낮아졌으며 포장의 냉해정도와도 시기에 관계없이 고도의 부의 상관을 인정할수 있었으며 3일째에서 가장 높았다. 5. 고로 발아후 3일에 나타난 결과에 의거 Amylase 활력의 차로 내한성의 강, 중, 약을 구명하는 것이 가장 알맞은 것으로 보이며 내한성판별의 기준으로써 효율적 편법으로 인정되었다.

• 한국응용곤충학회지
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• 제14권4호
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• pp.214-214
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• 1975

This study was conducted to evaluated the nature of the variety Tongil(Suweon 213-1) in resistance to the striped rice borer, Chilo suppressalis Walker, comparing with those of Rexoro(susceptible check) and TKM-6(resistant check) selected at IRRI. 1. The striped rice borer moths much more preferred the variety Tongil for oviposition than the varieties Rexoro and TKM-6. The variety Tongil and more egg masses and number of eggs than the varieties Rexoro and TKM-6, while TKM-6 having more egg masses and more number of eggs than Rexoro. This reaction was consistent throughout the test regardless of the number of tillers per hill. 2. In laboratory, preference of larvae for feeding was studied with 5cm of stem pieces of the varieties. The results showed, in contrast to the case of ovipositional preference, that the striped rice borer larvae least preferred the stems of Tongil among the test varieties, while larvae much more preferred the stems of Rexoro than those of TKM-6. 3. The larval weights at 20 days later infested on the 40 day-old plants were the lowest on Tongil among the test varieties. On the variety Rexoro the larvae had heavier body weights(43.0mg), higher pupation(64.9%) and higher adult emergence(83.3%) than those on Tongil(larval weights 30.3mg, pupation 60%, adult emergence 60.7%) and TKM-6(larval weights 35.7mg, pupation 56.3%, adult emergence 51.9%). The pupal weights, however, were not consistent among the test varieties and/or sexes in comparison with the larval weights, pupation and adult emergence above mentioned. 4. Field experiments indicated that the incidence of dead hearts at 70 days after transplanting was relatively higher on the variety Tongil(11.1%) than those on Rexoro(8.9%) and TKM-6(8.4%), and the incidence of white heads at harvest was, in contrast to the dead hearts, lower on Tongil(9.8%) than those on Rexoro(27.4%) and TKM-6(13.9%). At harvest lower larval survival observed on Tongil (49 larvae/40 hills) than those on the susceptible variety Rexoro(104 larvae/40 hills) and on the resistant variety TKM-6(70 larvae/40 hills). The average larval weights collected from three test varieties at harvest were 80.5mg from Tongil, 83.7mg from TKM-6 and 99.6mg from Rexoro. 5. Increased nitrogen fertilizer application to the variety Tongil, the striped rice borer damage was increased. Also, preference of larvae for feeding significantly increased with the increase of nitrogen fertilizer application. 6. Any specific association between the plant characters and striped rice borer resistance could not be found. The variety Tongil even having large number of tillers, short plant height, large stem, broad leaf, etc, had still high preference of moths for oviposition, low preference of larvae for feeding, low damage, and relatively high antibiosis. 7. Resistance of the variety Tongil to the striped rice borers seemed to be associated with the low feeding preference and the relatively high antibiosis, not associated directly with the ovipositional preference.

• 한국작물학회지
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• 제50권3호
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• pp.151-155
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• 2005

1970년대 이후 육성된 우리나라 벼품종들에 대하여 벼흰잎마름병 레이스 K1, K2, K3 및 K3a에 대한 반응을 검토한 결과를 요약하면 다음과 같다. 1. 통일형인 51품종의 저항성 정도를 검정한 결과, 레이스 K1에 저항성 13품종, 레이스 K1과 K2에 저항성 9품종, 레이스 K1, K2, 및 K3에 저항성 1품종, 레이스 K1, K2, K3 및 K3a모두에 저항성을 보인 것은 14품종이었다. 2. 자포니카형인 158품종의 저항성 반응을 살펴 본 결과, 레이스 K1 저항성 31품종, 레이스 K1과 K2에 저항성 1품종, 레이스 K1, K2 및 K3에 저항성 28품종, 레이스 K1, K2, K3 및 K3a모두에 저항성 1품종이었다. 3. 레이스 K1, K2, K3 및 K3a에 저항성인 품종은 통일형 품종이 자포니카형 품종 보다 훨씬 많았다.

• 한국응용곤충학회지
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• 제7권
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• pp.21-25
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• 1969

전국적으로 발생하며 대두에 많은 피해를 주고 있는 콩씨스트 선충의 방제에 보다 능률적인 효과를 얻기 위하여 본선충의 피해를 구명하고 저항성 대두 품종을 선발하여 품종육성에 기여코자 장려품종 8품종을 포함 한 64품종을 공시 저항성을 검정하였다. 1. 장단백목을 공시하여 콩씨스트선충에 대한 피해(감수율)를 조사한 결과, 접종시험(Pot)에서 $y=36.2-0.63\times(r=-0.74)$이었고, 포장시험에서는 $y=10.3-0.32\times (r=-0.56)$ 으로서 선충의 밀교와 대두 수양과는 부의 상관관계가 있었다. 2. 장려품종은 모두 저항성에 약 하였고 PI-84751, PI-90763, Southern-Proripic, 금강소입, 백태등은 비교적 강하였으며 그외의 모든 공시 품종들은 중간성일 감수성으로 나타났다. 3. 저항성 품종과 감수성 품종 間에 선충의 발육에 차가 있었고 특히 여성충수에 있어서 차가 많았다

• 원예과학기술지
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• 제29권4호
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• pp.336-344
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• 2011

Using the abundant available information about the tomato genome, we developed DNA markers that are linked to disease resistant loci and performed marker-assisted selection (MAS) to construct multi-disease resistant lines and varieties. Resistance markers of Ty-1, T2, and I2, which are linked to disease resistance to Tomato yellow leaf curl virus (TYLCV), Tomato mosaic virus (ToMV), and Fusarium wilt, respectively, were developed in a co-dominant fashion. DNA sequences near the resistance loci of TYLCV, ToMV, and Fusarium wilt were used for primer design. Reported candidate markers for powdery mildew-resistance were screened and the 32.5Cla marker was selected. All four markers (Ty-1, T2, I2, and 32.5Cla) were converted to cleavage amplification polymorphisms (CAPS) markers. Then, the CAPS markers were applied to 96 tomato lines to determine the phenetic relationships among the lines. This information yielded clusters of breeding lines illustrating the distribution of resistant and susceptible characters among lines. These data were utilized further in a MAS program for several generations, and a total of ten varieties and ten inbred lines were constructed. Among four traits, three were introduced to develop varieties and breeding lines through the MAS program; several cultivars possessed up to seven disease resistant traits. These resistant trait-related markers that were developed for the tomato MAS program could be used to select early stage seedlings, saving time and cost, and to construct multi-disease resistant lines and varieties.

• The Plant Pathology Journal
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• 제18권4호
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• pp.179-186
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• 2002

Four newly developed cotton mutants (M-111, M-7662, M-358 and M-218) were compared for their resistance against Cotton leaf curl virus(CLCuV) together with commercial resistant (CIM-443, CIM-482, CIM-473, FH-900 and FH-901) and susceptible (5-12) varieties by artificial inoculation through grafting and under natural field conditions. Infectivity and success of grafting were 100% in all cases. None of the grafted plants were found immune or asymptomatic. All the grafted mutants and most of their single plant progeny rows (SPPRs) showed highly resistant responses as the symptoms displayed by these mutants were milder than the commercial cultivars. Grafted mutants also had delayed disease reactions as they took more time (25-30 days) to produce disease symptoms, as compared with resistant commercial varieties that produced disease 18-22 days after inoculation. Growth of the grafted SPPRs of tested mutants was normal, which is an indication that there will be no production losses. Observations under natural infestation of whitefly showed that two SPPRs of M-ll/CE and M-7662-1/2 and one resistant variety CIM-443 exhibited slight incidence of disease, while one SPPR of M-l1/59 and S-12 were moderately susceptible and highly susceptible with 21％ and 97.l％ disease incidence, respectively. This study also showed that plants displaying more disease symptoms through grafting were easily infected under natural conditions. These results suggest that preference should be given to those plants that exhibited highly resistant responses after artificial inoculation.

• 한국연초학회지
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• 제22권1호
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• pp.7-12
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• 2000

This study was conducted to identify useful germ plasm sources of resistance to black shank, Phytophthora. parasitica, in Nicotiana tabacum and to characterize the interaction between host plant resistance to black shank and pathogen variability. The six internationally represen-tative set of black shank resistant and susceptible tobacco genotypes and four Korean lines were tested for black shank on the field infested with P. parasitica at Chonju Experiment Station, Korea Ginseng & Tobacco Research Institute during the 1992～1997 growing season. Almost 100% of the plants of the most susceptible genotype, Ky 14, did not survive. Complete survival of (MS Ky 14 x L8)F$_1$, which is resistant to reee 0 and ausceptible to reace 1, suggests that Race 0 is the only race of the P. parasitica in this bield. Coker 371 Gold and Beinhart 1000-1 having excellent resistance to black shank could be recommended as the parental cultivars for improving black shank resistance in Korea. Data of the diseased plants showed that KB 101 and KB 110 were medium high resistant and KB 103 and KB 108 being low resistant to black shank.

• 한국응용곤충학회지
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• 제17권1호
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• pp.53-63
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• 1978

본 연구는 도작해충으로서 문제화되고 있는 벼멸구, 흰등멸구, 끝동매미중에 대한 저항성정도를 구명하고 저 전국각도 수도장려품종을 포함한 주요품종 40품종과 최근에 육성된 우량계통 6계통을 공시하여 2-3령충의 약충을 접종 내성의 반응을 검토하고, 벼멸구에 대한 저항성품종과 감수성품종의 친화성을 조사한 바 다음과 같은 결과를 얻었다. 1. 저항성품종 Mudgo와 감수성품종 만경에서의 벼멸구 사육일수에 따른 충체의 증가량은 접종당시에 비하여 접종후 15일에 Mudgo에서는 2배정도였으나 만경에서는 3배이상의 증체량을 보였다. 2. 감수성품종(만경)과 저항성품종(Mudo)에서의 벼멸구 사육일수별 생충율과 사충율 조사에서 접종후 15일에 사충율이 Mudgo $74.0\%$ 만경 $31.2\%$로서 현저한 차이가 있었다. 3. 벼멸구에 대한 저항성검정에서는 우량계통의 KR 108-243-1, HR529-41-3-2가 고위저항성 반응을 보였고 기타 대부분의 품종들이 감수성 반응을 보였으나 적나, 사도미노리, IR24, IR8 등이 중도감수성 반응이었다. 4. 흰등멸구에 대한 반응에서는 적시 우량계통중의 KR108-243-1, KR109-154-2, HR529-45-3-2가 고도저항성이었고 장려품종 및 우량품종은 감수성이었으며, 수원 #82 통일, IR8, 팔금, 이리 309, 호광, 중국#31등이 중도감수성반응을 보였다. 5. 끝동매미충에 대한 검정에서는 우량계통의 KR 108-234-1, HR529-135-1이 저항성이었고, 벼멸구, 흰등멸구에 저항성인 Mudgo가 감수성이었고, 통일, 수원 82호, IR24, 밀도 중국 31호가 중도감수성 반응이었으며 기타 품종들은 고도감수성이었다. 6. 본시험의 결과 KR108-243-1이 공시된 멸구, 매미충류에 저항성반응이었으며 감수성이기는 하나 비교적 벼멸구, 흰등멸구에 IR8, 벼멸구, 끝동매미충에 IR24의 Indica 항총들이 흰등멸구, 끝동매미충에 통일, 수원 82호, 중국 31호가 중도감수성이었다.