• 식물병연구
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• 제22권3호
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• pp.202-207
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• 2016

쌀은 세계에서 가장 중요한 작물 중의 하나이지만 충분한 비타민을 제공하지 않고, 벼 도열병과 흰잎마름병은 전 세계적으로 가장 큰 피해를 주고 있는 병해이다. 리보플라빈, 비타민 B2는 인간의 건강에 필수적인 영양소이며, 식물의 병원균에 대한 식물의 방어 활성화로 알려져 있다. 본 연구에서는 BioDoctor (Hyunnong Co., Ltd., Gokseong, Korea)라는 리보플라빈 기반 제품을 벼에 경엽살포했을 때, 주요 병해에 대한 저항성 유도 및 벼 체내에 비타민 함량이 증가되는지에 대한 가능성을 조사하였다. 온실검정에서 BioDoctor 제품 500배와 1,000배로 희석하여 경엽처리 결과 벼 도열병과 흰잎마름병에 대해 현저한 병 저항성을 유도하였다. 또한, BioDoctor를 처리한 알곡과 잎에서 통계적으로 유의한 수준으로 리보플라빈 함량이 검출되었다. 본 연구는 비타민인 리보플라빈이 함유된 BioDoctor가 벼 도열병과 흰잎마름병에 대해 병 저항성을 유도할 뿐만 아니라 쌀 곡류 내에 비타민 함량을 증가시키는 큰 잠재력을 가지고 있는 것으로 나타났다.

• 한국응용곤충학회지
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• 제22권2호
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• pp.74-83
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• 1983

Yield losses due to diseases and insect pests were mentioned and emphasized the efficiency of resistant cultivars in curving the yield losses and increasing chemical efficiency. Present status of resistance breeding for blast, bacterial leaf blight viruses, brown planthopper and white backed planthopper were introduced and the resistance sources for those are discussed. Breeding strategies for above items were presented. Specially for the blast resistance, discussions were made in some detail. With brief future prospects of resistance breeding in Korea, a suggestion was made for pathologists to make clear about whether the blast spores will be brought from mainland China as we see with Bph and Wbph or not.

• Plant Breeding and Biotechnology
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• 제7권3호
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• pp.272-286
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• 2019

Developing elite hybrid rice varieties is one important objective of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F₁ hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F₁ hybrid combinations and two control vatieties. Results showed that nine F₁ hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F₁ hybrid rice varieties with resistance to disease/insect were selected from eleven F₁ hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F₁ hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

• The Plant Pathology Journal
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• 제28권4호
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• pp.364-372
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• 2012

Xanthomonas oryzae pv. oryzae causing bacterial leaf blight disease in rice produces and secretes Hpa1 protein that belongs to harpin protein family. Previously it was reported that Hpa1 induced defense responses when it was produced in tobacco. In this study, we expressed hpa1 gene in rice and Arabidopsis to examine the effects of Hpa1 expression on disease resistance to both fungal and bacterial pathogens. Expression of hpa1 gene in rice enhanced disease resistance to both X. oryzae pv. oryzae and Magnaporthe grisea. Interestingly, individual transgenic rice plants could be divided into four groups, depending on responses to both pathogens. hpa1 expression in Arabidopsis also enhanced disease resistance to both Botrytis cineria and Xanthomonas campestris pv. campestris. To examine genes that are up-regulated in the transgenic rice plants after inoculation with X. oryzae pv. oryzae, known defense-related genes were assessed, and also microarray analysis with the Rice 5 K DNA chip was performed. Interestingly, expression of OsACS1 gene, which was found as the gene that showed the highest induction, was induced earlier and stronger than that in the wild type plant. These results indicate that hpa1 expression in the diverse plant species, including monocot and dicot, can enhance disease resistance to both fungal and bacterial plant pathogens.

• 한국육종학회지
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• 제41권2호
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• pp.92-100
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• 2009

To investigate the mutagenecity of sodium azide (SA) and to select mutants having various agronomic characteristics in rice (Oryza sativa L. ssp. japonica), dry seeds of rice variety 'Suweon 472' were treated with 0, 0.5, 1.0, 2.0, and 4.0 mM SA solutions prepared in 0.1 M phosphate buffer (pH 3.0). Germination rate, seedling height and sterility were investigated in $M_1$ generation and chlorophyll mutations were observed in $M_3$ generation. Germination rate and seedling height decreased as the increase of SA concentration in $M_1$ generation, the maximum seed sterility (40.8%) was found at 4.0 mM SA concentration. Chlorophyll mutants were occurred in $M_3$ generation and the frequency calculated on a line basis was 13.5% at the same treatment. Many kinds of mutations for morphological and agronomic characters were observed and mutations with short culm and glabrous leaf were frequently found in $M_3$ generation. Interestingly, five mutant lines resistant to blast or bacterial blight (BB) were selected and evaluated with several isolates in $M_3$ generation although Suweon 472 has been known to be susceptible to blast and BB. These mutants showed all resistance to seven isolates of blast and a total of 76 lines among 2,567 lines evaluated showed resistant to race K1 of bacterial blight. Two mutant lines (440172 and 41272) showed different reaction to BB isolates from the other resistant mutants. A few kinds of endosperm mutants were also identified and most of them were waxy mutants.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.67.2-68
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• 2003

A rice cultivar, Taebaegbyeo, is highly resistant to rice blast and moderately resistant to bacterial leaf blight (BLB) caused by Magnaporthe grisea and Xanthomonas oryzae pv. oryzae, respectively. To study the rice disease resistance mechanism, we generated rice deletion M3 mutants by gamma-ray irradiation. Blast and BLB responses of 16,000 M3 mutants were screened by inoculating mixtures of 4 races (KJ-201, H-1113a, KI-313, KI-409) of M. grisea and 3 Korean races of X. oryzae pv. oryzae. We selected so far 21 M3 mutants of Taebaegbyeo showing high susceptibility to the diseases. One of the mutants, KCT-6417, was susceptible to KI-1113a race of M. grisea, suggesting the deletion of a race-specific blast resistance gene in the mutant. To isolate rice genes involved in blast resistance and defense response, we take a PCR-based suppression subtractive hybridization approach using cDNAs of blast-inoculated wild type and the KCT-6417 as a tester and a driver, respectively. Genes specifically expressed in the wild type will be presented. The selected genes would give us a clue to understand mechanism for the race specific resistance and defense responses against M. grisea H-1113a in Taebaegbyeo.

• 식물병연구
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• 제25권3호
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• pp.108-113
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• 2019

북한의 작물 생산성은 남한에 비하여 월등히 낮다. 이러한 이유 중 하나는 취약한 병 관리에 있다. 북한은 농경지의 약 30%가 논으로 이루어져 있다. 벼 도열병과 벼 흰잎마름병은 북한 벼 재배 시 각각 가장 중요한 곰팡이 병 및 세균 병이다. 본 연구에서는 벼 도열병균과 벼 흰잎마름병균에 대한 7개 북한 벼 품종의 병 저항성 반응을 시험하였다. 남한에서 분리한 18개 벼 도열병균에 대한 7개 벼 품종의 반응은 다소 다르게 나타났다. 7개 벼 품종 중 길주1호는 KI 혹은 KJ 레이스에 관계없이 남한에서 분리한 벼 도열병균 18개 균주 모두에 매우 저항성을 보였다. 평도5호는 남한에서 분리한 벼 도열병균 15개 균주에 대하여 감수성 반응을 보였다. 반면, 벼 흰잎마름병균에 대한 북한 벼 품종의 병 저항성 반응은 벼 도열병균에 대한 반응과 차이를 보였다. 길주1호는 K1 레이스인 KXO42와 K2 레이스인 KXO90에 대하여 높은 감수성을 보였고, 평도5호는 K1 레이스인 KXO85와 K3 레이스인 KXO19에 완전한 저항성을 보였다. 평양21호는 K1 레이스 모두에 완전한 저항성을 보였고, 원산69호는 KXO85를 제외한 모든 벼 흰잎마름병균에 감수성을 보였다. 이 연구는 향후 남북 농업 협력을 통하여 북한 벼 품종을 남한에 도입 시 두 병원균에 대한 북한 벼 품종의 저항성 반응 검정 및 벼 품종 육성에 중요한 기본 자료가 될 것이다.

• 한국육종학회지
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• 제51권3호
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• pp.222-233
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• 2019

'해품'은 농촌진흥청 국립식량과학원에서 우리나라 중만생종에 편중된 벼 농사의 문제점을 완화하여 수확기 노동력 분산, 수확 후 관리의 유연성 확보, 이모작 재배의 확대를 위해 품질, 수량, 재배안정성이 향상된 중생종 벼 품종을 개발하고자 육성되었다. 벼흰잎마름병 저항성 고품질 '익산493호'(품종명 '진백')를 모본으로 하고 복합내병충성 '익산495호'('다청')와 단간 내 도복 직파적응성 '익산496호'('중모1005')를 교배한 F₁을 부본으로 삼원교배한 F₁을 약배양하여 계통육성과정, 생산력 검정시험 및 지역적응성 검정시험을 거쳐 육성되었다. '해품'은 보통기보비재배에서 평균 출수기 8월 11일로 '남평'에 비해 3일 정도 빠른 중생종이다. '해품'은 간장이 73 cm로 '남평'보다 8 cm 작은 단간 품종으로 도복에 안정적인 특성을 나타내며, '남평'과 비슷한 내수발아성을 갖추고 있어 중생종 품종의 남부평야지 재배 시 수발아 피해를 경감시키는데 도움을 줄 것으로 생각된다. '해품'은 벼흰잎마름병 저항성 유전자 Xa3과 xa5 두 개가 집적되어 있어 병원성이 강한 벼흰잎마름병균 K3a균계까지 저항성이며 줄무늬잎마름병에 강하고 도열병에 중도저항성을 나타내는 복합 내병성 품종이다. '해품'은 중만생종에 비해 수확기가 빠른 중생종임에도 보통기 보비재배에서 '남평'과 비슷한 수량성을 나타냈으며, 이모작 재배시험에 적용 가능한 수량성을 나타냈다. '해품'은 쌀의 외관품위가 좋고 밥맛이 우수하여 최고품질 품종에 선정되는 등 중생종 품종 및 벼흰잎마름병 저항성 품종의 품질 향상에 기여하였다. '해품'은 최고품질에 적정수량성을 확보하고 있으며 내수발아성, 벼흰잎마름병 저항성 등 재배안정성이 향상된 중생종 품종으로 남부평야지 및 벼흰잎마름병 발병상습지 재배에 적합하다(품종보호권 등록번호: 제6068호, 2016. 5. 3.).

• 한국육종학회지
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• 제40권3호
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• pp.344-347
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• 2008

"눈보라" 품종은 작물과학원 호남농업연구소에서 2006년도에 육성한 내병 다수성 찰벼 품종으로 주요특성과 수량성을 요약하면 다음과 같다. 1. 출수기는 중부평야 및 호남평야의 보통기 보비재배에서 8월 11일로 신선찰보다 2일 정도 늦은 중생종이다. 2. 찰벼로 쌀알은 백도가 높고 도정율은 신선찰벼와 비슷하며 알카리 붕괴도가 높아 호화가 잘되는 편이다. 3. 도열병 및 흰잎마름병 레이스 $K_1{\sim}K_3$에는 저항성 반응을 보였으나 줄무늬 잎마름병 멸구 및 매미충류에는 약한 반응을 보였다. 4. 유묘 내냉성은 강하며 신선찰벼보다 출수지연일수는 길지만 임실율은 약간 높은 편이다. 5. 쌀 수량은 보통기 보비재배에서 5.34 MT/ha로 신선찰벼보다 4% 증수되었으나 이모작 재배 및 만식재배에서는 남평벼보다 8 9%감수되었다. 6. "눈보라"는 중부이남 평야지 및 북부중산간지, 충남이남 평야지 1모작답에 알맞은 품종이다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.266-266
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• 2022

The outbreaks of blast, bacterial blight and viral diseases have been increasing in early maturing rice cultivating areas in the central northern regions, recently. As the occurrence of sudden insects pests and disasters increases due to global climate warming, it is urgent to develop a variety of disaster-tolerant, high-quality varieties in response. This study was carried out to elucidate the characteristics of early-maturing, high-quality and multiple disease resistant rice variety, Cheolweon109 that was adapted to cultivation in the mid-mountainous regions of the central northern regions. Cheolweon109 was derived from a cross between Suweon546, medium maturing variety, and Sangju44 which is early maturing and resistant to blast, bacterial blight and rice stripe virus. The heading date of Cheolweon109 was July 30, 3 days later than Odae. The culm length of Cheolweon109 was 79 cm, which was about 5 cm taller than Odae, and the ripening ratio was 85.1%, which was 10% higher than that of Odae. This variety had 5.54 MT/ha of milled rice productivity, which was 99% of the Odae. Although Cheolweon109 was tall, it was strong against lodging. It was strong against bacterial blight (K1, K2, K3 race), rice stripe virus, and the pre-harvest sprouting which rate was 2.4%. The appearance of the grains of rice was clean, the glossiness was 70.6, and the head rice ratio was 95.3% high. Because Cheolweon109 had superior disease resistance, disaster resistance, and high quality than Odae, it was expected that can be used to expand the diversity of early maturing and high-quality rice varieties in central northern regions.