Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)

The Korean Society of Plant Pathology (한국식물병리학회)
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Rice blast susceptible mutants of Taebaegbyeo and genes differentially expressed in he wild type rice.

  • Lee, C. H. (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • C. U. Han (Biological Function Research Team, Korea Research Institute of Chemical Technology, Dept. Agricultural Biology, Chungbuk National University) ;
  • K. S. Jang (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Park, Y. H. (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • H. K. Lim (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Kim, J.C. (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Park, G. J. (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • J.S. Cha (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Park, J. E. (Dept. Crop Science, Chung-Nam National University)
  • Published : 2003.10.01
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Abstract

A rice cultivar, Taebaegbyeo, is highly resistant to rice blast and moderately resistant to bacterial leaf blight (BLB) caused by Magnaporthe grisea and Xanthomonas oryzae pv. oryzae, respectively. To study the rice disease resistance mechanism, we generated rice deletion M3 mutants by gamma-ray irradiation. Blast and BLB responses of 16,000 M3 mutants were screened by inoculating mixtures of 4 races (KJ-201, H-1113a, KI-313, KI-409) of M. grisea and 3 Korean races of X. oryzae pv. oryzae. We selected so far 21 M3 mutants of Taebaegbyeo showing high susceptibility to the diseases. One of the mutants, KCT-6417, was susceptible to KI-1113a race of M. grisea, suggesting the deletion of a race-specific blast resistance gene in the mutant. To isolate rice genes involved in blast resistance and defense response, we take a PCR-based suppression subtractive hybridization approach using cDNAs of blast-inoculated wild type and the KCT-6417 as a tester and a driver, respectively. Genes specifically expressed in the wild type will be presented. The selected genes would give us a clue to understand mechanism for the race specific resistance and defense responses against M. grisea H-1113a in Taebaegbyeo.

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