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Comparison of Growth and Water Quality in Juvenile Japanese Eel, Anguilla Japonica Fed Commercial Extruded Pellet and Paste Type Diets (시판 뱀장어용 부상사료 및 반죽사료에 대한 치어기 뱀장어(Anguilla japonica) 성장 및 사육수질 비교)

  • Kim, Seoung-Won;Rim, Sang-Koo;Sohn, Sang-Gyu;Lee, Jinhwan
    • Journal of Fisheries and Marine Sciences Education
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    • v.20 no.1
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    • pp.90-94
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    • 2008
  • Growth and water quality in Japanese eel, Anguilla japonica ($6.0{\pm}0.9g$) fed extruded and paste type diets were compared for 8 weeks. Fish in the duplicate tanks were fed with one of three experimental diets: two different extruded pellet diets and one paste type diet. Six FRP tanks were used with stocking density of 3.6 kg per tank (3 m3). Restricted feeds (ca. 2% of body weight) were served twice daily. Weight gains were checked at the end of every two-week interval, and water qualities in terms of NH4-N and NO2-N were determined daily one hour after feeding in the morning. Water temperature, dissolved oxygen, and pH were constantly kept within the optimum range, while fifty percent of total water volume was changed daily. Eels fed the extruded pellet diets showed significantly better (P<0.05) growth performance than did eels fed the paste type diet. The amount of feed waste from the paste type diet was significantly greater than those from the extruded pellet diets (P<0.05). Ammonia (NH4-N) waste was significantly higher from the paste diet than from the extruded pellet diets (P<0.05). These results show that extruded pellet diets are better than the paste type diet for growth and water quality management in eel culture.

Effects of type of magnet attachment and implant angulation in two implant overdenture models

  • Song, So-Yeon;Kang, Kyeong-Hwan;Lee, Jeong-Yol;Shin, Sang-Wan
    • The Journal of Advanced Prosthodontics
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    • v.12 no.1
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    • pp.33-37
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    • 2020
  • PURPOSE. The purpose of this study is to evaluate the effects of type of magnet attachment and implant angulation in two implant overdenture models. MATERIALS AND METHODS. Magnet attachments used in this study were flat and dome types (MGT5515, MGT5520D, Dentium Co., Seoul, Korea). Two implants with keepers were inserted in the resin blocks at a distance of 24 mm. For the first model, the implants were parallel to the vertical and perpendicular to the horizontal; for the second model, both were angulated 5 degrees to the mesial; for the third model, both were angulated 10 degrees toward the mesial. The retentive force was measured in both vertical and lateral directions. Statistical analyses were performed using SPSS software version 22.0 (α=.05). RESULTS. The flat type magnet attachment showed the highest lateral retentive force in the 20° divergent group (P<.05) and the dome type magnet attachment showed the highest lateral retentive force in the parallel group (P<.05). The vertical and lateral retentive force of the dome type magnet attachment was greater than that of the flat type magnet attachment in every direction (P<.05). CONCLUSION. Within the limitations of this study, the dome shape magnet attachment can resist vertical and lateral retentive force more superiorly than the flat type magnet attachment, regardless of angle, in the mandibular two implant model.

Joining Ability and Mechanical Properties of Friction Stir Lap Welded A5052-H112 Alloy (A5052-H112 합금의 겹치기 마찰교반접합 건전성)

  • Ko, Young-Bong;Choi, Jun-Woong;Park, Kyeung-Chae
    • Journal of Welding and Joining
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    • v.28 no.1
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    • pp.34-40
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    • 2010
  • In Friction Stir Lap Welding(FSLW), the movement of material within the weld was more important than the microstructure, due to the interface present between the sheets. Thus, The soundness of free defect, Effective Sheet Thickness(EST) and width of joint were most important factor of mechanical properties. Specimens by lap joint types that were 'A-type' and 'R-type' were made in this study. A-type tensile specimen was loaded at advancing side and R-type tensile specimen was loaded at retreating side. Macro-, micro-structural observation and mechanical properties of FSLW A5052-H112 alloy ware investigated under varying rotating and welding speed. The results were as follows: Material hook formed decreasing after sharply increasing was appeared at the end interface of joint area in advanced side, and material hook formed decreasing after smoothly increasing was observed at that in retreated side. Tensile load had no relation with defects. As rotating speed was higher, tensile strength was increasing and EST was decreasing regardless of joint types. joint efficiency was over 70%. In a result of fractography, fracture in A-type was partially occurred by dimple in SZ, and fracture in R-type was generally occurred by dimple in HAZ.

A Study on the Development of PMM Algorithms for a Product Development Innovation (제품 개발 혁신을 위한 PPM 알고리즘에 관한 연구)

  • Lee, Jae-Myung;Lee, Hong-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.7
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    • pp.1750-1759
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    • 2009
  • Recently from the enterprise when about reduction the product of numerous type to develop does not produce simultaneously with Life Cycle shortening and JIT productions of the product, is confronting in the environment which will not become. In order to confront in like this environment, developing the good product importance but, the product researches what kind of direction and developing with core subject of technical innovation is recognized. For the development method and an investment direction of the product proposed PPM (Product Portfolio Management) algorithms from the research which sees from like this viewpoint. With PPM algorithms which are proposed, divided the product in 6 kind groups of standardization type, discrimination type, material cost investigation type, processing cost investigation type and sale promotion type and abolition type and presented tyresearch and development direction..Research approach portfolio investigation of existing research and consulting experience of research and development field with background, presented the analytical model of algorithm, is applied of the instance which composed.

Effects of Annealing and Neutron Irradiation on Micostructural and Mechanical Properties of High Burn-up Zr Claddings (고연소도 신형 Zr피복관의 미세조직과 기계적 특성에 미치는 열처리 및 중성자 조사의 영향)

  • Baek, Jong Hyuk;Kim, Hyun Gil;Jeong, Yong Hwan
    • Journal of the Korean Society for Heat Treatment
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    • v.17 no.3
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    • pp.151-164
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    • 2004
  • The changes of microstructural and mechanical properties were evaluated for the high burn-up fuel claddings after the neutron irradiation of $1.8{\sim}3.1{\times}10^{20}n/cm^2$ (E>1.0 MEV) in HANARO research reactor. After the irradiation, the spot-type dislocations (a-type dislocations) were easily observed in most claddings, and the density of the dislocations was different depending on the grains and was higher at grain boundaries than within grains. As the final annealing temperature increased, the density of spot-type dislocations increased and the line-type dislocations (c-type dislocations) which was perpendicular to the <0002> direction, appeared sporadically in some claddings. However, the types of precipitates in the fuel claddings after the irradiation were not changed from that in unirradiated claddings. The mechanical properties including the hardness, strength and elongation after the irradiation were changed due to the formation of spot-type dislocations. That is, the increase in hardness and strength as well as the decrease in elongation after the irradiation was occurred simultaneously with increasing the final annealing temperature. Owing to the Nb contribution to the formation of spot-type dislocation during the irradiation, the increase in hardness and strength in higher Nb-contained Zr alloys after the irradiation was higher than that in lower Nb-contained Zr alloys.

Development of Anti-Rolling Demo System for Mobile Harbor Using Maglev Type AMD (자기부상방식 AMD를 이용한 모바일 하버용 횡동요 저감 데모 장비의 개발)

  • Park, Cheol-Hoon;Ham, Sang-Yong;Kim, Byung-In;Lee, Sung-Whee;Park, Hee-Chang;Cho, Han-Wook;Moon, Seok-Jun;Chung, Tae-Young
    • Journal of the Korean Society for Precision Engineering
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    • v.28 no.1
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    • pp.40-47
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    • 2011
  • Mobile harbor which is a novel concept of ocean transportation to bring the containers from the cargo ship waiting on the ocean away is being focused now. To provide the mobile harbor with the stable loading/unloading condition, it is necessary to develop the oscillation mitigation technologies such as anti-rolling system. Anti-rolling system using AMD(Active Mass Driving) has merits that it can handle the disturbances more actively and mitigate the rolling oscillation faster than other type anti-rolling system. However, rack-and-pinion type AMD has problems such as big friction noise from gears and motor, wear and tear, and continuous maintenance. In this paper, novel anti-rolling system using Maglev type AMD for mobile harbor is suggested in order to resolve the problems caused by the friction. This novel anti-rolling system doesn't make any friction because it supports the moving mass by using magnetic levitation force and moves it by using propulsion force from the linear motor. The demo system of the novel anti-rolling system using maglev type AMD has been developed in order to investigate its feasibility. This paper presents the procedures and results of development of this demo system.

Production of Recombinant Human Papillomavirus Type 52 L1 Protein in Hansenula polymorpha Formed Virus-Like Particles

  • Liu, Cunbao;Yao, Yufeng;Yang, Xu;Bai, Hongmei;Huang, Weiwei;Xia, Ye;Ma, Yanbing
    • Journal of Microbiology and Biotechnology
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    • v.25 no.6
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    • pp.936-940
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    • 2015
  • Human papillomavirus (HPV) type 52 is a high-risk HPV responsible for cervical cancer. HPV type 52 is common around the world and is the most common in some Asian regions. The available prophylactic HPV vaccines protect only from HPV types 16 and 18. Supplementing economical vaccines that target HPV type 52 may satisfactorily complement available prophylactic vaccines. A codon-adapted HPV 52 L1 gene was expressed in the methylotrophic yeast Hansenula polymorpha, which is used as an industrial platform for economical hepatitis B surface antigen particle production in China. We found that the recombinant proteins produced in this expression system could form virus-like particles (VLPs) with diameters of approximately 50 nm. This study suggests that the HPV 52 VLPs produced in this platform may satisfactorily complement available prophylactic vaccines in fighting against HPVs prevalent in Asia.

Development of an attenuated vaccine strain from a korean respiratory type infectious bronchitis virus (한국호흡기형 닭전염성기관지염 생독백신주의 작성)

  • Choi, Kang-Seuk;Jeon, Woo-Jin;Lee, Eun-Kyoung;Kye, Soo-Jeong;Park, Mi-Ja;Kwon, Jun-Hun
    • Korean Journal of Veterinary Research
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    • v.51 no.3
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    • pp.193-201
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    • 2011
  • An attenuated vaccine strain AVR1/08 of Korean respiratory type of infectious bronchitis virus (IBV) was developed by 89th passages of IBV D85/06 strain in chicken eggs. The AVR1/08 strain had higher virus titer at least 20 times ($10^{1.3}$) than the parent virus D85/06 by egg inoculation method. The AVR1/08 strain had a single point mutation (S to Y) at position 56 of spike protein of IBV compared to parent virus IBV D85/06 strain. The mutation was observed consistently at viruses after 47th passage in chicken eggs. The AVR1/08 strain showed no virulence even after 6 passages in chickens and all chickens inoculated induced anti-IBV antibody 14 days after vaccination. The AVR1/08 strain had broad protective efficacy against QX type Korean nephropathogenic virus (Q43/06 strain), KM91 type Korean nephropathogenic virus (KM91 strain) and Korean respiratory virus (D85/06 strain). In contrast, Massachusetts (Mass) type attenuated vaccine strain H120 showed protection of 37.5 to 50% against these three viruses. Our results indicate that the AVR1/08 strain has potential as an attenuated vaccine effective in controlling IBVs circulating in Korea.

Determination of Neurotoxin Gene Expression in Clostridium botulinum Type A by Quantitative RT-PCR

  • Shin, Na-Ri;Shin, Ji-Hun;Chun, Jeong Hoon;Yoon, So-Yeon;Kim, Bong Su;Oh, Hee-Bok;Rhie, Gi-eun
    • Molecules and Cells
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    • v.22 no.3
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    • pp.336-342
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    • 2006
  • Real time reverse transcription (RT)-PCR was used to quantify the expression of the botulinum neurotoxin type A (BoNT/A) gene (cntA) by normalization with the expression of 16S rRNA. The method were confirmed by monitoring the mRNA levels of cntA during growth in five type A strains. In all but one of the strains the expression of cntA mRNA was maximal in the late exponential phase, and approximately 35-fold greater than in the early exponential phase. The concentration of the extracellular BoNT/A complex detected by ELISA was highest in stationary phase. Sodium nitrite and sorbic acid completely inhibited growth at 20 ppm and $4mg\;ml^{-1}$, respectively. CntA expression became lower in proportion to the concentration of sorbic acid, and this reduction was confirmed by mouse bioassay. Our results show that real time RT-PCR can be used to quantify levels of C. botulinum type A neurotoxin transcripts and to assess the effects of food additives on botulinal risk.

Effect of Storage Conditions, Rice, Cooker and Oil Types on the Changes of Resistant Starch Contents of Cooked Rice (저장조건, 쌀, 조리기구와 유지 종류가 밥의 저항전분 함량 변화에 미치는 영향)

  • Ren, Chuanshun;Kim, Ji Myoung;Park, Sara;Jeong, On Bit;Shin, Malshick
    • Korean journal of food and cookery science
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    • v.32 no.1
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    • pp.9-15
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    • 2016
  • The changes of resistant starch (RS) contents of cooked rice with soybean and coconut oils under different storage conditions were investigated and RS contents were compared between the rice and cooker types. The japonica (Hopyeong) and the indica (Thailand) type rice were cooked (washed rice: water = 100: 130) using an electric cooker and a saucepan. The coconut oil and soybean oil (3%, based on rice, w/w) were added into cooking water before heating. The RS contents of freeze-dried cooked rice powders (newly-cooked rice, stored for 12 h in the refrigerator, microwave heating after storage for 12 h in the refrigerator) were measured by the AOAC method. The RS contents of cooked rice using a saucepan were higher than those using an electric cooker. The indica type cooked rice had a higher RS content than the japonica type cooked rice, regardless of storage conditions. However, addition of oil before cooking rice resulted in increased RS content on storage in the refrigerator. The highest RS content of the cooked indica type rice with soybean oil ($5.89{\pm}0.22%$) that was stored for 12 h in the refrigerator was analyzed. The results suggested that the cooked rice formed retrograded (RS3) and amylose-lipid complex (RS5) type RS; furthermore, the RS content is affected by storage conditions, rice, cooker and oil types.