Browse > Article
http://dx.doi.org/10.14405/kjvr.2011.51.3.193

Development of an attenuated vaccine strain from a korean respiratory type infectious bronchitis virus  

Choi, Kang-Seuk (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Jeon, Woo-Jin (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Lee, Eun-Kyoung (Veterinary Medicine and Biologics Division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Kye, Soo-Jeong (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Park, Mi-Ja (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Kwon, Jun-Hun (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Publication Information
Korean Journal of Veterinary Research / v.51, no.3, 2011 , pp. 193-201 More about this Journal
Abstract
An attenuated vaccine strain AVR1/08 of Korean respiratory type of infectious bronchitis virus (IBV) was developed by 89th passages of IBV D85/06 strain in chicken eggs. The AVR1/08 strain had higher virus titer at least 20 times ($10^{1.3}$) than the parent virus D85/06 by egg inoculation method. The AVR1/08 strain had a single point mutation (S to Y) at position 56 of spike protein of IBV compared to parent virus IBV D85/06 strain. The mutation was observed consistently at viruses after 47th passage in chicken eggs. The AVR1/08 strain showed no virulence even after 6 passages in chickens and all chickens inoculated induced anti-IBV antibody 14 days after vaccination. The AVR1/08 strain had broad protective efficacy against QX type Korean nephropathogenic virus (Q43/06 strain), KM91 type Korean nephropathogenic virus (KM91 strain) and Korean respiratory virus (D85/06 strain). In contrast, Massachusetts (Mass) type attenuated vaccine strain H120 showed protection of 37.5 to 50% against these three viruses. Our results indicate that the AVR1/08 strain has potential as an attenuated vaccine effective in controlling IBVs circulating in Korea.
Keywords
attenuation; infectious bronchitis; live vaccine; protection;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Ammayappan A, Upadhyay C, Gelb J Jr, Vakharia VN. Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI. Arch Virol 2009, 154, 495-499.   DOI   ScienceOn
2 Cavanagh D. Coronavirus avian infectious bronchitis virus. Vet Res 2007, 38, 281-297.   DOI   ScienceOn
3 Cavanagh D, Elus MM, Cook JKA. Relationship between sequence variation in the S1 spike protein of infectious bronchitis virus and the extent of crossprotection in vivo. Avian Pathol 1997, 26, 63-74.   DOI   ScienceOn
4 Cavanagh D, Naqi SA. Infectious bronchitis. In: Saif YM (ed.). Diseases of Poultry. 11th ed. pp. 101-119, Iowa State Press, Ames, 2003.
5 Choi KS, Lee EK, Jeon WJ, Park MJ, Kim JW, Kwon JH. Pathogenicity and antigenicity of a new variant of Korean nephropathogenic infectious bronchitis virus. J Vet Sci 2009, 10, 357-359.   DOI   ScienceOn
6 Collisson EW, Pei J, Dzielawa J, Seo SH. Cytotoxic T lymphocytes are critical in the control of infectious bronchitis virus in poultry. Dev Comp Immunol 2000, 24, 187-200.   DOI   ScienceOn
7 Cook JKA, Orbell SJ, Woods MA, Huggins MB. Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes. Avian Pathol 1999, 28, 477-485.   DOI   ScienceOn
8 Geerligs HJ, Boelm GJ, Meinders CAM, Stuurman BGE, Symons J, Tarres-Call J, Bru T, Vila R, Mombarg M, Karaca K, Wijmenga W, Kumar M. Efficacy and safety of an attenuated live QX-like infectious bronchitis virus strain as a vaccine for chickens. Avian Pathol 2011, 40, 93-102.   DOI   ScienceOn
9 Hewson KA, Ignjatovic J, Browning GF, Devlin JM, Noormohammadi AH. Infectious bronchitis viruses with naturally occurring genomic rearrangement and gene deletion. Arch Virol 2011, 156, 245-252.   DOI   ScienceOn
10 Hofstad MS. Cross-immunity in chickens using seven isolates of avian infectious bronchitis virus. Avian Dis 1981, 25, 650-654.   DOI   ScienceOn
11 Huang YP, Wang CH. Development of attenuated vaccines from Taiwanese infectious bronchitis virus strains. Vaccine 2006, 24, 785-791.   DOI   ScienceOn
12 Huang YP, Wang CH. Sequence changes of infectious bronchitis virus isolates in the 3' 7.3 kb of the genome after attenuating passage in embryonated eggs. Avian Pathol 2007, 36, 59-67.   DOI   ScienceOn
13 Jackwood MW, Hilt DA, Brown TP. Attenuation, safety and efficacy of an infectious bronchitis virus GA98 serotype vaccine. Avian Dis 2003, 47, 627-632.   DOI   ScienceOn
14 Jang JH, Sung HW, Song CS, Kwon HM. Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea. J Vet Sci 2007, 8, 401-407.   DOI   ScienceOn
15 Kim JH. Serological differentiation, pathogenicity and immunogenicity of avian bronchitis viruses isolated in Korea. PhD thesis. Seoul National University, Seoul, 1994.
16 Kim JH, Song CS, Mo IP, Kim SH, Seong HW, Yun HS. An outbreak of nephropathogenic infectious bronchitis in commercial pullets. Res Rep Rural Dev Adm (Suweon) 1992, 34, 28-31.
17 Lee EK, Jeon WJ, Lee YJ, Jeong OM, Choi JG, Kwon JH, Choi KS. Genetic diversity of avian infectious bronchitis virus isolates in Korea between 2003 and 2006. Avian Dis 2008, 52, 332-337.   DOI   ScienceOn
18 Lee HJ, Youn HN, Kwon JS, Lee YJ, Kim JH, Lee JB, Park SY, Choi IS, Song CS. Characterization of a novel live attenuated infectious bronchitis virus vaccine candidate derived from a Korean nephropathogenic strain. Vaccine 2010, 28, 2887-2894.   DOI   ScienceOn
19 Lee SK, Sung HW, Kwon HM. S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea. Arch Virol 2004, 149, 481-494.   DOI   ScienceOn
20 Lim TH, Lee HJ, Lee DH, Lee YN, Park JK, Youn HN, Kim MS, Lee JB, Park SY, Choi IS, Song CS. An emerging recombinant cluster of nephropathogenic strains of avian infectious bronchitis virus in Korea. Infect Genet Evol 2011, 11, 678-685.   DOI   ScienceOn
21 Liu S, Zhang X, Gong L, Yan B, Li C, Han Z, Shao Y, Li H, Kong X. Altered pathogenicity, immunogenicity, tissue tropism and 3'-7kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos. Vaccine 2009, 27, 4630-4640.   DOI   ScienceOn
22 Nix WA, Troeber DS, Kingham BF, Keeler CL Jr, Gelb J Jr. Emergence of subtype strains of the Arkansas serotype of infectious bronchitis virus in Delmarva broiler chickens. Avian Dis 2000, 44, 568-581.   DOI   ScienceOn
23 MacDonald JW, McMartin DA. Observations on the effects of the H52 and H120 vaccine strains of infectious bronchitis virus in the domestic fowl. Avian Pathol 1976, 5, 157-173.   DOI
24 Mase M, Tsukamoto K, Imai K, Yamaguchi S. Phylogenetic analysis of avian infectious bronchitis virus strains isolated in Japan. Arch Virol 2004, 149, 2069-2078.
25 McKinley ET, Jackwood MW, Hilt DA, Kissinger JC, Robertson JS, Lemke C, Paterson AH. Attenuated live vaccine usage affects accurate measures of virus diversity and mutation rates in avian coronavirus infectious bronchitis virus. Virus Res 2011, 158, 225-234.   DOI   ScienceOn
26 Park JY, Park SI, Sung HW, Kim JH, Song CS, Lee CW, Kwon HM. Variations in the nucleocapsid protein gene of infectious bronchitis viruses isolated in Korea. Virus Genes 2005, 31, 153-162.   DOI   ScienceOn
27 Picault JP, Drouin P, Guittet M, Bennejean G, Protais J, L'Hospitalier R, Gillet JP, Lamande J, Bachelier A. Isolation, characterisation and preliminary cross-protection studies with a new pathogenic avian infectious bronchitis virus (strain PL-84084). Avian Pathol 1986, 15, 367-383.   DOI
28 Reed LJ, Muench H. A simple method of estimating fifty per cent endpoints. Am J Hyg 1938, 27, 493-497.
29 Rhee YO, Kim JH, Kim JH, Mo IP, Youn HJ, Choi SH, Namgoong S. Outbreaks of infectious bronchitis in Korea. Korean J Vet Res 1986, 26, 277-282.
30 Seo SH, Wang L, Smith R, Collisson EW. The carboxyl-terminal 120-residue polypeptide of infectious bronchitis virus nucleocapsid induces cytotoxic T lymphocytes and protects chickens from acute infection. J Virol 1997, 71, 7889-7894.
31 Song CS, Kim JH, Lee YJ, Kim SJ, Izumiya Y, Tohya Y, Jang HK, Mikami T. Detection and classification of infectious bronchitis viruses isolated in Korea by dot-immunoblotting assay using monoclonal antibodies. Avian Dis 1998, 42, 92-100.   DOI   ScienceOn
32 Song CS, Lee YJ. Molecular and epidemiological characteristics of infectious bronchitis virus isolated in Korea. Korean J Poult Sci 2000, 27, 91-98.
33 Sun C, Han Z, Ma H, Zhang Q, Yan B, Shao Y, Xu J, Kong X, Liu S. Phylogenetic analysis of infectious bronchitis coronaviruses newly isolated in China, and pathogenicity and evaluation of protection induced by Massachusetts serotype H120 vaccine against QX-like strains. Avian Pathol 2011, 40, 43-54.   DOI   ScienceOn