• International Journal of Oral Biology
• /
• v.42 no.2
• /
• pp.39-45
• /
• 2017

Metformin (1,1-dimethylbiguanide hydrochloride), derived from French lilac (Galega officinalis), is a first-line anti-diabetic drug prescribed for patients with type 2 diabetes. However, the role of metformin in odontoblastic cell differentiation is still unclear. This study therefore undertook to examine the effect of metformin on regulating odontoblast differentiation in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. As compared to controls, metformin significantly accelerated the mineralization, significantly increased and accelerated the expressions of ALP and Col I mRNAs, and significantly increased the accelerated expressions of DSPP and DMP-1 mRNAs, during differentiation of MDPC-23 cells. There was no alteration in cell proliferation of MDPC-23 cells, on exposure to metformin. These results suggest that the effect of metformin on MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells, facilitates the odontoblast differentiation and mineralization, without altering the cell proliferation.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2003.04a
• /
• pp.126-126
• /
• 2003

Sucrose-phosphate synthase (SPS) is a key regulatory enzyme in sucrose synthesis. To investigate the role of SPS in carbon partitioning, we produced transgenic rice plants overexpressing a cyanobacterial SPS from Synechocystis sp. PCC 6803. The gene was expressed under the control of the maize Ubil promoter in transgenic plants. Southern and Northern blot analyses confirmed the integration and the expression of the transgene in four transgenic rice lines. All of the four transgenic! lines analyzed showed abnormal vegetative and reproductive developments. Analysis of SPS activities and primary metabolites in the transgenic rice plants will be presented.

• Bulletin of the Korean Chemical Society
• /
• v.29 no.5
• /
• pp.1025-1032
• /
• 2008

(4,5-Dichloro-6-oxo-6H-pyridazin-1-yl)phosphoric acid diethyl ester (3a) are efficient and selective coupling agents for the amidation of carboxylic acids. Amidation of aliphatic and aromatic carboxylic acids with aliphatic and aromatic amines using 3a under mild condition gave chemoselectively the corresponding amides in good to excellent yield. Three protected-dipeptides were also synthesized from N-BOC-Phe and O-Me-amino acid hydrochlorides using 3a under mild condition.

• The Korean Journal of Physiology and Pharmacology
• /
• v.16 no.3
• /
• pp.199-204
• /
• 2012

We evaluated the role of Tat-mediated p66shc transduction on the activation of endothelial nitric oxide synthase in cultured mouse endothelial cells. To construct the Tat-p66shc fusion protein, human full length p66shc cDNA was fused with the Tat-protein transduction domain. Transduction of TAT-p66shc showed a concentration- and time-dependent manner in endothelial cells. Tat-mediated p66shc transduction showed increased hydrogen peroxide and superoxide production, compared with Tat-p66shc (S/A), serine 36 residue mutant of p66shc. Tat-mediated p66shc transduction decreased endothelial nitric oxide synthase phosphorylation in endothelial cells. Furthermore, Tat-mediated p66shc transduction augmented TNF-${\alpha}$-induced p38 MAPK phosphorylation in endothelial cells. These results suggest that Tat-mediated p66shc transduction efficiently inhibited endothelial nitric oxide synthase phosphorylation in endothelial cells.

• Advances in environmental research
• /
• v.6 no.4
• /
• pp.301-315
• /
• 2017

An Amberlite XAD-4 resin impregnated with di(2-ethylhexyl)phosphoric acid was prepared and its adsorption-desorption behaviors with Sr(II) ions under various conditions was examined. The resin was characterized by fourier transform infrared and thermal analysis techniques. The effects contact time, temperature, pH, interfering ions and eluants were studied. Results showed that adsorption of Sr (II) well fitted with pseudo-second-order kinetic model. The equilibrium adsorption data of Sr (II) on the impregnated resin were analyzed by Jossens, Weber-van Vliet, Redlich-Peterson and Fritz-Schlunder models to find out desirable equilibrium condition. Among them, the Fritz-Schlunder model best fitted to the experimental data. The maximum sorption capacity of impregnated resin amounted to 0.45 mg/ g at pH 8.0 and $20^{\circ}C$.

• Journal of Information Display
• /
• v.5 no.1
• /
• pp.28-33
• /
• 2004

A high voltage NMOSFET is proposed to drive top emission organic light emitting device (OLED) used in the organic electroluminescent (EL) display on the single crystal silicon substrate. The high voltage NMOSFET can be fabricated by utilizing a simple layout technique with a standard CMOS logic process. It is clearly shown that the maximum supply voltage ($V_{DD}$) required for the pixel-driving transistor could reach 45 V through analytic and experimental methods. The high voltage NMOSFET was fabricated by using a standard 1.5 ${\mu}m$, 5 V CMOS logic process. From the measurements, we confirmed that the high voltage NMOSFET could sustain the excellent saturation characteristic up to 50 V without breakdown phenomena.

• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.67-67
• /
• 2003

• 한국약용작물학회:학술대회논문집
• /
• 2008.11a
• /
• pp.275-276
• /
• 2008

• 한국약용작물학회:학술대회논문집
• /
• 2008.05a
• /
• pp.269-270
• /
• 2008

• Korean Journal of Radiology
• /
• v.23 no.2
• /
• pp.291-291
• /
• 2022