Browse > Article
http://dx.doi.org/10.4196/kjpp.2012.16.3.199

Tat-Mediated p66shc Transduction Decreased Phosphorylation of Endothelial Nitric Oxide Synthase in Endothelial Cells  

Lee, Sang-Ki (Infection Signaling Network Research Center, Research Institute of Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
Lee, Ji-Young (Infection Signaling Network Research Center, Research Institute of Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
Joo, Hee-Kyoung (Infection Signaling Network Research Center, Research Institute of Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
Cho, Eun-Jung (Infection Signaling Network Research Center, Research Institute of Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
Kim, Cuk-Seong (Infection Signaling Network Research Center, Research Institute of Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
Lee, Sang-Do (Infection Signaling Network Research Center, Research Institute of Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
Park, Jin-Bong (Infection Signaling Network Research Center, Research Institute of Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
Jeon, Byeong-Hwa (Infection Signaling Network Research Center, Research Institute of Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.16, no.3, 2012 , pp. 199-204 More about this Journal
Abstract
We evaluated the role of Tat-mediated p66shc transduction on the activation of endothelial nitric oxide synthase in cultured mouse endothelial cells. To construct the Tat-p66shc fusion protein, human full length p66shc cDNA was fused with the Tat-protein transduction domain. Transduction of TAT-p66shc showed a concentration- and time-dependent manner in endothelial cells. Tat-mediated p66shc transduction showed increased hydrogen peroxide and superoxide production, compared with Tat-p66shc (S/A), serine 36 residue mutant of p66shc. Tat-mediated p66shc transduction decreased endothelial nitric oxide synthase phosphorylation in endothelial cells. Furthermore, Tat-mediated p66shc transduction augmented TNF-${\alpha}$-induced p38 MAPK phosphorylation in endothelial cells. These results suggest that Tat-mediated p66shc transduction efficiently inhibited endothelial nitric oxide synthase phosphorylation in endothelial cells.
Keywords
p66shc; Tat-mediated transduction; Endothelial nitric oxide synthase; Superoxide; Endothelial cells;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 Shin W, Cuong TD, Lee JH, Min B, Jeon BH, Lim HK, Ryoo S. Arginase inhibition by ethylacetate extract of caesalpinia sappan lignum contributes to activation of endothelial nitric oxide synthase. Korean J Physiol Pharmacol. 2011;15:123-128.   DOI   ScienceOn
2 Lee SK, Kim YS, Kim CS, Son SJ, Yoo DG, Lee KH, Lee SD, Park JB, Jeon BH. p66shc adaptor protein suppresses the activation of endothelial nitric oxide synthase in mouse embryonic fibroblasts. Korean J Physiol Pharmacol. 2006;10:155-159.
3 Grethe S, Ares MP, Andersson T, Pörn-Ares MI. p38 MAPK mediates TNF-induced apoptosis in endothelial cells via phosphorylation and downregulation of Bcl-x(L). Exp Cell Res. 2004;298:632-642.   DOI   ScienceOn
4 Yu JH, Kim CS, Yoo DG, Song YJ, Joo HK, Kang G, Jo JY, Park JB, Jeon BH. NADPH oxidase and mitochondrial ROS are involved in the TNF-alpha-induced vascular cell adhesion molecule-1 and monocyte adhesion in cultured endothelial cells. Korean J Physiol Pharmacol. 2006;10:217-222.
5 Li G, Barrett EJ, Barrett MO, Cao W, Liu Z. Tumor necrosis factor-alpha induces insulin resistance in endothelial cells via a p38 mitogen-activated protein kinase-dependent pathway. Endocrinology. 2007;148:3356-3363.   DOI   ScienceOn
6 Zhang XH, Yokoo H, Nishioka H, Fujii H, Matsuda N, Hayashi T, Hattori Y. Beneficial effect of the oligomerized polyphenol oligonol on high glucose-induced changes in eNOS phosphorylation and dephosphorylation in endothelial cells. Br J Pharmacol. 2010;159:928-938.   DOI   ScienceOn
7 Xing F, Jiang Y, Liu J, Zhao K, Mo Y, Liu Z, Zeng Y. Downregulation of human endothelial nitric oxide synthase promoter activity by p38 mitogen-activated protein kinase activation. Biochem Cell Biol. 2006;84:780-788.   DOI   ScienceOn
8 Yamamori T, White AR, Mattagajasingh I, Khanday FA, Haile A, Qi B, Jeon BH, Bugayenko A, Kasuno K, Berkowitz DE, Irani K. P66shc regulates endothelial NO production and endothelium-dependent vasorelaxation: implications for age-associated vascular dysfunction. J Mol Cell Cardiol. 2005;39:992-995.   DOI   ScienceOn
9 Lee SK, Chung JI, Park MS, Joo HK, Lee EJ, Cho EJ, Park JB, Ryoo S, Irani K, Jeon BH. Apurinic/apyrimidinic endonuclease 1 inhibits protein kinase C-mediated p66shc phosphorylation and vasoconstriction. Cardiovasc Res. 2011;91:502-509.   DOI   ScienceOn
10 Lee SK, Kim HS, Song YJ, Joo HK, Lee JY, Lee KH, Cho EJ, Cho CH, Park JB, Jeon BH. Alteration of p66shc is associated with endothelial dysfunction in the abdominal aortic coarctation of rats. FEBS Lett. 2008;582:2561-2566.   DOI   ScienceOn
11 Angkeow P, Deshpande SS, Qi B, Liu YX, Park YC, Jeon BH, Ozaki M, Irani K. Redox factor-1: an extra-nuclear role in the regulation of endothelial oxidative stress and apoptosis. Cell Death Differ. 2002;9:717-725.   DOI   ScienceOn
12 Becker-Hapak M, McAllister SS, Dowdy SF. TAT-mediated protein transduction into mammalian cells. Methods. 2001;24:247-256.   DOI   ScienceOn
13 Song YJ, Lee JY, Joo HK, Kim HS, Lee SK, Lee KH, Cho CH, Park JB, Jeon BH. Tat-APE1/ref-1 protein inhibits TNF-alphainduced endothelial cell activation. Biochem Biophys Res Commun. 2008;368:68-73.   DOI   ScienceOn
14 Lee JY, Park KS, Cho EJ, Joo HK, Lee SK, Lee SD, Park JB, Chang SJ, Jeon BH. Human HOXA5 homeodomain enhances protein transduction and its application to vascular inflammation. Biochem Biophys Res Commun. 2011;410:312-316.   DOI   ScienceOn
15 Wender PA, Mitchell DJ, Pattabiraman K, Pelkey ET, Steinman L, Rothbard JB. The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: peptoid molecular transporters. Proc Natl Acad Sci USA. 2000;97:13003-13008.   DOI   ScienceOn
16 Francia P, delli Gatti C, Bachschmid M, Martin-Padura I, Savoia C, Migliaccio E, Pelicci PG, Schiavoni M, Luscher TF, Volpe M, Cosentino F. Deletion of p66shc gene protects against age-related endothelial dysfunction. Circulation. 2004;110:2889-2895.   DOI   ScienceOn
17 Wu Z, Rogers B, Kachi S, Hackett SF, Sick A, Campochiaro PA. Reduction of p66Shc suppresses oxidative damage in retinal pigmented epithelial cells and retina. J Cell Physiol. 2006;209:996-1005.   DOI   ScienceOn
18 Anderson TJ. Nitric oxide, atherosclerosis and the clinical relevance of endothelial dysfunction. Heart Fail Rev. 2003;8:71-86.   DOI   ScienceOn
19 Cho EJ, Park MS, Kim SS, Kang G, Choi S, Lee YR, Chang SJ, Lee KH, Lee SD, Park JB, Jeon BH. Vasorelaxing activity of ulmus davidiana ethanol extracts in rats: activation of endothelial nitric oxide synthase. Korean J Physiol Pharmacol. 2011;15:339-344.   DOI   ScienceOn
20 Kim DD, Sanchez FA, Duran RG, Kanetaka T, Duran WN. Endothelial nitric oxide synthase is a molecular vascular target for the Chinese herb Danshen in hypertension. Am J Physiol Heart Circ Physiol. 2007;292:H2131-2137.   DOI   ScienceOn
21 Migliaccio E, Mele S, Salcini AE, Pelicci G, Lai KM, Superti-Furga G, Pawson T, Di Fiore PP, Lanfrancone L, Pelicci PG. Opposite effects of the p52shc/p46shc and p66shc splicing isoforms on the EGF receptor-MAP kinase-fos signalling pathway. EMBO J. 1997;16:706-716.   DOI   ScienceOn
22 Stocker R, Keaney JF Jr. Role of oxidative modifications in atherosclerosis. Physiol Rev. 2004;84:1381-1478.   DOI   ScienceOn
23 Molavi B, Mehta JL. Oxidative stress in cardiovascular disease: molecular basis of its deleterious effects, its detection, and therapeutic considerations. Curr Opin Cardiol. 2004;19:488-493.   DOI   ScienceOn
24 Madamanchi NR, Vendrov A, Runge MS. Oxidative stress and vascular disease. Arterioscler Thromb Vasc Biol. 2005;25:29-38.
25 Napoli C, Martin-Padura I, de Nigris F, Giorgio M, Mansueto G, Somma P, Condorelli M, Sica G, De Rosa G, Pelicci P. Deletion of the p66Shc longevity gene reduces systemic and tissue oxidative stress, vascular cell apoptosis, and early atherogenesis in mice fed a high-fat diet. Proc Natl Acad Sci USA. 2003;100:2112-2116.   DOI   ScienceOn
26 Kim CS, Son SJ, Kim EK, Kim SN, Yoo DG, Kim HS, Ryoo SW, Lee SD, Irani K, Jeon BH. Apurinic/apyrimidinic endonuclease1/redox factor-1 inhibits monocyte adhesion in endothelial cells. Cardiovasc Res. 2006;69:520-526.   DOI   ScienceOn
27 Camici GG, Schiavoni M, Francia P, Bachschmid M, Martin-Padura I, Hersberger M, Tanner FC, Pelicci P, Volpe M, Anversa P, Luscher TF, Cosentino F. Genetic deletion of p66(Shc) adaptor protein prevents hyperglycemia-induced endothelial dysfunction and oxidative stress. Proc Natl Acad Sci USA. 2007;104:5217-5222.   DOI   ScienceOn
28 Kawashima S. Malfunction of vascular control in lifestylerelated diseases: endothelial nitric oxide (NO) synthase/NO system in atherosclerosis. J Pharmacol Sci. 2004;96:411-419.   DOI   ScienceOn
29 Martin-Padura I, de Nigris F, Migliaccio E, Mansueto G, Minardi S, Rienzo M, Lerman LO, Stendardo M, Giorgio M, De Rosa G, Pelicci PG, Napoli C. p66Shc deletion confers vascular protection in advanced atherosclerosis in hypercholesterolemic apolipoprotein E knockout mice. Endothelium. 2008;15:276-287.   DOI   ScienceOn