• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.2
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• pp.137-142
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• 2004

In this study, a simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (CP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene of Streptomyces avidinii. Confocal fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. The results suggest that this strategy, which consists of a combination of $\mu$CP and cSA-fused proteins. is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications. one such being the use in protein-protein assays.

• 대한생식의학회:학술대회논문집
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• 2004.06a
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• pp.85-85
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• 2004

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.10a
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• pp.407-408
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• 2006

• Bulletin of the Korean Chemical Society
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• v.29 no.1
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• pp.127-129
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• 2008

EPR spectroscopic technique was applied for a quantitative analysis of Eu(II) for a speciation of europium in a LiCl-KCl eutectic melt. By adopting the first absorption line of each isotopes (151Eu and 153Eu), a calibration plot was obtained. The calibration of the EPR intensity shows a good linearity according to the amount of Eu(II). The EPR intensity was identified to increase proportionally with a decrease of the attenuation parameter for EPR microwave power. The fluorescence technique was used qualitatively to find whether either of Eu(II) or Eu(III) ions exists in a molten salt sample. The ICP-AES technique was also adopted to determine the total concentration of europium in the sample, since EPR is only sensitive for detecting the Eu(II) ion. The extent of the reduction of Eu(III) in the LiCl-KCl eutectic melt at 723 K was determined by using this technique.

• Journal of fish pathology
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• v.22 no.3
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• pp.353-366
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• 2009

We constructed a rock bream Oplegnathus fasciatus leukocyte cDNA library and a total of 795 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 795 EST clones, 491 different ESTs showed significant homology to previously described genes while 304 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 121 different sequences were identified as putative bio-defense genes or genes associated with immune response.

• Bulletin of the Korean Chemical Society
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• v.31 no.4
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• pp.921-924
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• 2010

Blocking of IL-6 has been postulated to be an effective therapy in the pathogenesis of several inflammatory diseases. The current study was performed to examine the potential effects of alkamides isolated from P. longum and P. nigrum on IL-6 induced Stat3 activation and identify the structure-activity relationship of these alkamides in human hepatoma cells. Among 10 alkamides isolated from P. longum and P. nigrum, compounds 6, 7 and 9 were identified as strong inhibitors of IL-6 action, which inhibit IL-6 induced Stat3-dependent luciferase activities. These inhibitory activities were positively influenced by the presence of piperidine moiety.

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.859-865
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• 2008

Bacterial glycosyltransferases have drawn growing attention as economical enzymes for oligosaccharide synthesis, with their easy expression and relatively broad substrate specificity. Here, we characterized a glycosyltransferase homolog (Fnu_GT) from a human oral pathogen, Fusobacterium nucleatum. Bioinformatic analysis showed that Fnu_GT belongs to the glycosyltransferases family II. The recombinant Fnu_GT (rFnu_GT) expressed in Escherichia coli displayed the highest glycosylation activity when UDP-galactose (Gal) was used as a donor nucleotide-sugar with heptose or N-acetylglucosamine (GlcNAc) as an acceptor sugar. Interestingly, rFnu_GT transferred the galactose moiety of UDP-Gal to a nonreducing terminal GlcNAc attached to the trimannosyl core glycan, indicating its potential as an enzyme for human-type N-glycan synthesis.

• Proceedings of the Malacological Society of Korea Conference
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• 2005.07a
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• pp.19-19
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• 2005

• Proceedings of the Malacological Society of Korea Conference
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• 2005.07a
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• pp.20-20
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• 2005

• The Transactions of the Korean Institute of Power Electronics
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• v.26 no.5
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• pp.315-324
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• 2021

A solid state transformer (SST) that can interface an MVAC of three-phase 13.2 kV and a 1.5 kV DC distribution. SST consists of an AC/DC converter and a DC/DC converter with a high-frequency isolation transformer (HFIT). The AC/DC converter consists of cascaded NPC full-bridge to cope with the MVAC. The DC/DC converter applies a quad active bridge (QAB) topology to reduce the number of the HFIT. Topology analysis and controller design for this specific structure are discussed. In addition, the insulation of HFIT used in DC/DC converters is considered. The discussion is validated using a 300 kVA three-phase SST prototype.