• Title/Summary/Keyword: Research Culture

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L-Cysteine Metabolism and the Effects on Mycelium growth of Streptomyces albidoflavus SMF301 in Submerged Culture

  • Lee, Kye-Joon;Kim, Jong-Woong;Kang, Sung-Kyun
    • Journal of Microbiology and Biotechnology
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    • v.4 no.3
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    • pp.159-164
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    • 1994
  • Myceliuml growth and spore formation of Streptomyces albidoflavus SMF301 in submerged culture were compared with the metabolism of cysteine. Cysteine added to the culture was metabolized by cysteine desulfhydrase (EC 4.4.1.1.) to produce ammonium ions, hydrogen sulfide, and pyruvate. The redox potential of the culture broth was lowered immediately as the result of the metabolism of cysteine, which caused a lag period of mycelium growth. However enhanced activities of pyruvate dehydrogenase and a-ketoglutarate dehydrogenase were confirmed in the culture containing cysteine, indicating that pyruvate was utilized to support further mycelium growth.

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The Effect of Environment Uncertainty and Local Infrastructure on the Firm Culture, Operations Performance and Marketing Performance (환경 불확실성과 지역인프라가 기업문화, 운영성과, 마케팅성과에 미치는 영향 : 대구·경북지역 중소기업을 중심으로)

  • Ju, Ki-Jung;Kim, Jang-Ho
    • Korean Management Science Review
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    • v.29 no.3
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    • pp.67-80
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    • 2012
  • This study analyzes the relationship among environment uncertainty, local infrastructure, flexible-open firm culture, operations performance and marketing performance focus on SMEs. This research has revealed that the relation among firm size, firm type, firm culture, operations performance and marketing performance as well. The findings show that firm has its culture which is preparing environment uncertainty and local infrastructure influence on forming firm culture. Change-oriented and leaning-oriented firm cultures affect operations performance and marketing performance. In conclusion, this study suggests implication and limitations for further research.

Assessment of the Knowledge Management Technology Implementation as a Function of Organizational Culture Orientations (조직 유형과 지식 경영시스템 성공도 관계에 대한 연구)

  • Jeong Duke H.;Park Hee J.;Ahn Seoung ho
    • Journal of Information Technology Applications and Management
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    • v.11 no.3
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    • pp.137-145
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    • 2004
  • The purpose of this research is to explore the possible relationship between the successful implementation of knowledge management technology and specific organizational culture orientations. Data used to test hypothesis derived for this research were obtained from 227 responders from the Organizational Culture Profile (OCP) survey instruments and 67 responders from the Knowledge Management Technology Profile (KMTP) survey instruments representing 26 separate organizations. The OCP provides a profile of an organization's culture orientations while the KMTP provides a profile of the organization's degree of the KM technology implementation success. The results of this research suggests that employees of organization which are more successful in implementing KM technology have identified organizational cultures that embody a healthy mixture of both production-oriented and people-oriented attributes indicative of the culture orientation.

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Nutrients Removal of Municipal Wastewater and Lipid Extraction with Microalgae (조류를 이용한 하수고도처리 및 지질추출)

  • Park, Sangmin;Kim, Eunseok;Jheong, Weonhwa;Kim, Geunsu;Ahn, Kyunghee;Han, Jinseok;Kwon, Ohsang
    • Journal of Korean Society on Water Environment
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    • v.28 no.6
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    • pp.796-803
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    • 2012
  • Potential feasibility of nutrients removal and biofuel production with microalgae was evaluated in batch culture. Distribution of microalgae in fresh water including reservoir and river was investigated to search for the species with high content of lipid that could converted into biofuel. Green algae, Chlorella and Scenedesmus sp., these are known as species containing high lipid content for biodiesel production, were observed in both summer and autumn season. However another highly lipid-containing species, botryococcus sp. was not observed in this study. In mixed culture of microalgae using synthesized wastewater medium, green algae were found to be dominant, comparing to other species of diatoms and blue-green algae. And microalgae were also capable of removing nitrogen and phosphorus in batch experiments. During the culture period of 14 days, removal efficiencies of nitrate and phosphorus were 30% and 82%, respectively. Furthermore, content of the intracellular lipid extracted from algae cell was as favorable as 12-30% in the mixed culture where Scenedesmus and Chlorella sp. were dominant. Therefore the mixed culture of microalgae could be applied to biofuel production and tertiary wastewater treatment, even though there are economic barriers to overcome.

Design and Implementation of Electronic Culture Atlas for Oversea Region Research (해외지역연구를 위한 전자문화지도의 설계 및 구현)

  • Kang, Ji-Hoon;Moon, Sang-Ho;Yu, Young-Jung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.5
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    • pp.1174-1180
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    • 2013
  • In recent years, there are many awareness and demand for digital convergence of the future. In information technology, especially, convergence with other studies such as regional studies, literature and humanities should be needed because information technology is closely related to real life. Culture atlas represents various aspects related to culture visually using points, arcs, and more in the map. Thus, it should be an available tool to survey cultures efficiently in digital environments. For oversea region study, especially Mediterranean region research, we suggest the way to apply electronic culture atlas in this paper. In detail, design and implementation a study on Electronic Culture Atlas for overseas area. Research results for oversea regional studies can be expressed visually by utilizing digital culture map implemented in this paper. Therefore, digital culture atlas should be used as convergence media between information technology and other studies such as regional studies, humanities and so on, tools for oversea regional studies, and exhibition of research results.

Induction of Growth Hormone by the Roots of Astragalus membranaceus in Pituitary Cell Culture

  • Kim, Chung-Sook;Ha, Hye-Kyung;Kim, Jin-Sook;Kim, Yun-Tai;Kwon, Sun-Chang;Park, Sie-Won
    • Archives of Pharmacal Research
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    • v.26 no.1
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    • pp.34-39
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    • 2003
  • The traditional Asian medicinal herb, roots of Astragalus (A.) membranaceus (Leguminosae), is used for many purposes, some of which are purported to stimulate the release of growth hormone in vivo. Extracts of A. membranaceus were tested to determine whether they stimulate the release of growth hormone in rat pituitary cell culture. A. membranaceus was extracted sequentially with 80% ethanol (fraction A), n-hexane (fraction B); the test compound from the herbal extraction was isolated using silica gel column chromatography and was identified with spectral data. Test compound was also extracted by traditional boiling water methods. Induction of growth hormone in pituitary cell culture was conducted with isolated compounds and extracted fractions of A. Radix (dried roots of A. membranaceus). The fraction A was not active in the rat pituitary cell culture, but the fraction B derived from the ethanol fraction stimulated the release of growth hormone in culture. Six compounds from fraction B (1-6) were isolated and identified previously. The compounds 1,2-benzendicarboxylic acid diisononylester (1), $\beta$-sitosterol (2), and 3-Ο-$\beta$-D-galactopyranosyl-$\beta$-sitosterol (5) did not induce growth hormone release in the culture. Formononetin (3), 9Z, 12Z-octadecadienoic acid (4), stigmast-4-en-6$\beta$-o1-3-one (6) and 98-E, a mixture of 1'-9, 12-octadecadienoic acid (Z,Z)-2',3'-dihydroxy-propylester (7) and 1'-hexadecanoic acid-2',3'-dihydroxy-propylester (8) stimulated the release of growth hormone in the rat pituitary cell culture significantly compared to the control. In conclusions, four compounds isolated from extracts of A. Radix induced growth hormone release in the rat pituitary cell culture. The 98-E isolate was the most active inducer of growth hormone release.

Safety Culture: A Retrospective Analysis of Occupational Health and Safety Mining Reports

  • Tetzlaff, Emily J.;Goggins, Katie A.;Pegoraro, Ann L.;Dorman, Sandra C.;Pakalnis, Vic;Eger, Tammy R.
    • Safety and Health at Work
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    • v.12 no.2
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    • pp.201-208
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    • 2021
  • Background: In the mining industry, various methods of accident analysis have utilized official accident investigations to try and establish broader causation mechanisms. An emerging area of interest is identifying the extent to which cultural influences, such as safety culture, are acting as drivers in the reoccurrence of accidents. Thus, the overall objective of this study was to analyze occupational health and safety (OHS) reports in mining to investigate if/how safety culture has historically been framed in the mining industry, as it relates to accident causation. Methods: Using a computer-assisted qualitative data analysis software, 34 definitions of safety culture were analyzed to highlight key terms. Based on word count and contextual relevance, 26 key terms were captured. Ten OHS reports were then analyzed via an inductive thematic analysis, using the key terms. This analysis provided a concept map representing the 50-year data set and facilitated the use of text framing to highlight safety culture in the selected OHS mining reports. Results: Overall, 954 references and six themes, safety culture, attitude, competence, belief, patterns, and norms, were identified in the data set. Of the 26 key terms originally identified, 24 of them were captured within the text. The results made evident two distinct frames in which to interpret the data: the role of the individual and the role of the organization, in safety culture. Conclusion: Unless efforts are made to understand and alter cultural drivers and share these findings within and across industries, the same accidents are likely to continue to occur.

Bottle Cultivation of Pleurotus ostreatus, Agrocybe aegerita and Ganoderma lucidum using Rice hull media

  • Lee, he-duck;Kim, hong-kyu;Kim, yong-gyun;Lee, ga-soon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2001.04a
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    • pp.44-46
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    • 2001
  • Rice hull was used as a additive in order to find the effect for incresing of mushroom growth and yield in Chungnam Provincial techinical institution. 1 Treatment of 80% rice hull in small Neutaribeosut mycelial grow duration is shorter about 11 days and yield increased about 7% than conventional culture. 2. In case of Chongpung Neutaribeosut bottle culture, mycelial growth duration is shorter about two to three days in additive of 30 to 80% rice hull compared to conventional but yield similar to conventional. 3. Treatment of 30% rice hull in Agrocybe aegerita bottle culture, mycelial growth and yield increased 6days and 6% than convrntional, respectively 4 Treatment additived of 30% to 40% rice hull in Ganoderma lucidum bottle culture, similar to 454ays demand in mycelial grow duration and 38g yield/bottle in conventioal culture methods.

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Production of Ginseng Saponins with Cell Culture(II) (세포배양에 의한 고려인삼 성분의 생산 연구(II))

  • Chi, Hyung-Joon;Shin, Kuk-Hyun;Kim, Hyun-Soo;Cho, Hee-Jae
    • Korean Journal of Pharmacognosy
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    • v.20 no.3
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    • pp.162-169
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    • 1989
  • Tissue culture of the roots of Panax ginseng was carried out to enhance the production of ginseng callus as well as to increase its contents of ginsenosides. A long cylinder type callus mass was formed when cultured IK callus by rotary shaking culture method, the growth ratio of the callus being 7.71 which was approximately 4 fold higher than those obtained by other culture methods. Ginsenosides $Rg_1$, Re and $Rb_1$ could be detected from the callus mass by TLC, however, their total contents were revealed to be approximately 9% compared to that of the fresh ginseng root equivalent by HPLC analysis,.

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The Mediation Effect of Trust and Communication on Relationship between Organizational Culture and Innovative Behavior: Focused on Manufacturing Business (조직문화와 혁신행동 간의 관계에서 조직신뢰, 의사소통의 매개효과: 제조업을 중심으로)

  • Jeong, Da-Un;Chun, Byung-June
    • Asia-Pacific Journal of Business
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    • v.10 no.1
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    • pp.39-54
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    • 2019
  • The purpose of this study is to research the effect of organizational culture on trust and communication. Also, the study explores whether trust and communication affects innovative behavior and the mediation effect on innovative behavior among organizational culture, trust and communication. This study analyzed the mediating effect with SEM using data from the HCCP in 2015. The results of the study are as follows. First, it was found that innovative, collective and rational culture positively influence but hierarchical culture negatively influence trust and communication. Second, trust and communication have a positive effect on innovative behavior and trust has a positive effect on communication. Third, the effect innovative, collective hierarchical and rational culture on innovative behavior is mediated through trust and communication. Therefore, this study suggests creating an innovative, relationship-based and goal-oriented organizational culture which increases trust and communication that facilitates innovative behavior.