• Title/Summary/Keyword: Research Culture

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Survival and Growth of the Purplish Washington Clam, Saxidomus purpuratus Spat Sowed in bottom and intermediate culture (개조개, Saxidomus purpuratus의 중간육성 및 씨뿌림된 치패의 성장과 생존)

  • Jin, Young-Guk;Oh, Bong-Se;Jung, Choon-Koo;Kim, Tae-Ik;Park, Min-Woo
    • The Korean Journal of Malacology
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    • v.27 no.3
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    • pp.199-204
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    • 2011
  • We studied possibility of the sowing culture at the bottom after intermediate culture as a method for raising the survival of the Purplish Washington clam, Saxidomus purpuratus. The pearl net ($35cm(W){\times}35cm(B){\times}20cm(H)$) for S. prupuratus spat during the period of intermediate culture (hanging culture) from April to October in 2010. The pearl net (100 inds./net) was installed at two stations (Namhae and Pohang) being about 3-4 m in water depth. After hanging culture, S. prupuratus spat sowed in the bottom of the Gangjin Bay. The survivals (%) of intermediate culture of this species at Namhae station and Pohang station were 73% and 74%, respectively. Daily growth rate of mean shell length and mean weight in Namhae station were higher than those in Pohang station. After sowing at the bottom of the Gangjin Bay, its survival (%) showed 73.98% in January 2011. These results suggested the possibility of the intermediate culture as hanging culture for raising survival rate of S. prupuratus.

Comparative Study of Seeding and Culture Methods to Vascular Smooth Muscle Cells on Biodegradable Scaffold

  • Kim, Dong-Ik;Park, Hee-Jung;Eo, Hyun-Seoun;Suh, Soo-Won;Hong, Ji-Hee;Lee, Min-Jae;Kim, Jong-Sung;Jang, In-Sung;Kim, Byung-Soo
    • Journal of Microbiology and Biotechnology
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    • v.14 no.4
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    • pp.707-714
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    • 2004
  • How to improve the cell culture method on scaffolds is important in the tissue engineering fileld. In this study, we optimized seeding and culture methods to vascular smooth muscle cells (VSMCs) on biodegradable polymer scaffold. The primary culture of VSMCs obtained from canine external jugular vein was accomplished by applying the explant-derived method. The primary cultured VSMCs were seeded into scaffolds and then cultured by using various different methods; static or dynamic seeding, static or dynamic culture. The difference in proliferative response of VSMCs was analyzed with an alamar blue assay. Cell-polymer construct was examined by histochemical method and scanning electron microscopy. Mesh type scaffold ($10 \times 10 \times0.4 mm$) was made of polyglycolic acid (PGA) suture thread. The PGA mesh type scaffold was 45% in porosity, and 0.03 g in weight. The primary cultured VSMCs were confirmed with immunohistochemical staining using monoclonal anti-$\alpha$-smooth muscle actin. The density and distribution of proliferated VSMCs within the scaffold and cellular adherence on the surface of the scaffold showed better results in the static seeding condition than in the dynamic condition. Under the same condition of seeding method as the static condition, the dynamic culture condition showed enhanced proliferation rates of the VSMCs when compared to the static culture condition. In conclusion, to improve the VSMCs proliferation in vitro, static seeding is better than the dynamic condition. In the culture condition, however, culture under the dynamic status is better than the static condition. This was a pilot study to manufacture artificial vascular vessel by tissue engineering.

Semantic Network Analysis on the Research Trends in The Society of Korean Performance Art and Culture (우리나라 공연문화 연구동향의 의미연결망 분석)

  • Hwang, Dong-Ryul;Kwon, Yae-Ji
    • (The) Research of the performance art and culture
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    • no.37
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    • pp.437-464
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    • 2018
  • This study used semantic network analysis to understand the academic identity and characteristics of the society of korean performance art and culture and to grasp the trend of the research. For this purpose, this study analyze the research trend of korean performance art and culture related papers based on 455 whole articles in the Journal of The Society of Korean Performance Art and Culture by the Korean Performance Art and Culture Association from 2000 to 2017. Through this research, the trends of The Society of Korean Performance Art and Culture in the period of time were identified, and the phenomenon of the performance culture field and the future development direction were suggested.

Detection of Lily Symptomless Virus Using RT-PCR Technique (RT-PCR 기법을 이용한 Lily Symptomless Virus의 검정)

  • Joung, Young-Hee;Jeon, Jae-Heung;Choi, Kyung-Hwa;Kim, Hyun-Soon;Joung, Hyouk
    • Korean Journal Plant Pathology
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    • v.12 no.2
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    • pp.187-190
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    • 1996
  • 백합으로부터 total RNA를 분리하여 LSV 외피단백질 유전자의 551 bp에 해당하는 특정 염기서열을 증폭할 수 있는 primer로 RT-PCR를 수행하였다. 그 결과 Lilium oriental hybrid cvs. Miani, Marco Polo, Casablanca, Le Reve 품종에서 551 bp의 DNA 절편이 증폭되었고 이 절편의 염기서열을 분석한 결과 LSV외피단백질 유전자의 일부임을 확인할 수 있었다. 그러므로 RT-PCR 방법으로, 실험에 사용하 s4품종 모두 LSV에 감염되어 있음을 알 수 있었다.

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Perceived Organizational Culture and Psychological Empowerment of Employees in Casino Companies (카지노 종사자의 조직문화 인식과 심리적 임파워먼트)

  • Lee, Youngran;Chang, Jihyun
    • The Journal of Industrial Distribution & Business
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    • v.8 no.5
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    • pp.65-75
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    • 2017
  • Purpose - The purpose of this study is to verify a role of organizational culture as antecedents to influence psychological empowerment of organizational members, focusing on service workers of casino companies. Therefore, this study sought to examine the question of each clan, adhocracy, market, and hierarchy culture that the workers perceive to influence their psychological empowerment. Thus, the current study examined the relationship between organizational culture and the psychological empowerment by empirical analysis. Research design, data, and methodology - This study chose four domestic casino companies that are run only for foreigners. Using an offline survey, it analyzed the questionnaire data of the 249 surveys collected from employees working in the sales and service sectors. Independent variables were four types of organizational culture: clan, adhocracy, market, and hierarchy. Dependent variables were four subcategories of psychological empowerment: meaning, competence, self-determination, and impact. As research methods, the study applied descriptive statistics, factor analysis, reliability analysis, correlation analysis, and multiple regression analysis, using SPSS 21.0 statistical program. Results - Among the organizational culture types, the clan and adhocracy cultures, which emphasize flexibility and autonomy, were relatively more important for the psychological empowerment of workers than the hierarchy and market cultures. Clan culture was the most important factor in terms of meaning and competence, and adhocracy culture was the most important factor in self-determination and impact. However, the hierarchy and market cultures that pursue control and stability were also cultural types that positively affected psychological empowerment of the workers. Hierarchy culture showed positive effects on meaning, competence, and impact except self-determination, and market culture had positive effect only on competency. Conclusions - The study found that organizational culture is an important predictor of psychological empowerment of the employees in casino companies and that important organizational culture types may be different for each sub-factor of psychological empowerment. It suggests that casino companies have to try to recognize and secure diverse organizational culture in order to activate psychological empowerment of their employees because they can provide quality service for customers. Therefore, it is necessary to create a harmonious and balanced culture between promoting flexible and autonomous organizational atmosphere, and stably controlling and operating the organization.

The Effect of Organizational Culture Types on Service Quality and Customer Satisfaction in the Military Hospital Organizations (군 의료기관의 조직문화 유형이 의료서비스 질과 고객만족에 미치는 영향)

  • Shin, Dong Sun;Lee, Jae Hee
    • Korea Journal of Hospital Management
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    • v.21 no.4
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    • pp.23-44
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    • 2016
  • We conducted this research for the purpose of examining the effects of organizational culture on service quality and customer satisfaction in the military hospital organizations in Korea. The research samples were collected from employees in 12 military hospitals belonged to the Armed Forces Command. For the analysis we used SPSS 18.0 and AMOS 20.0 program for conducting reliability tests, confirmatory factor analysis, and structural equation model analysis in order to find the relationship between the variables analyzed. The result of this study shows that the military hospitals in Korea have strong relationship-oriented culture, but weak hierarchy-oriented culture. Among the three groups of military hospitals, innovation-oriented culture and relationship-oriented culture were the strongest in the executive agency hospital group, task-oriented culture was the strongest in the group of the he frontline hospitals and hierarchy-oriented culture was the strongest in the group of the rear area hospitals. It was found that the executive agency hospitals have the strongest service quality and customer satisfaction among the hospital groups analyzed. This research also found that relationship-oriented culture and innovation-oriented culture have a direct positive(+) effect on service quality; hierarchy oriented culture has a direct negative(-) effect on service quality; task oriented culture has a direct positive(+) effect on customer satisfaction, and service quality mediated between organizational culture and customer satisfaction. The findings from this study suggests that implementing the dynamic strategies for organizational culture with focus on service qualities is needed given that customer satisfaction in the military hospitals is highly affected by service quality.

The implicit meaning of British fashion into English culture identity (영국의 문화 정체성이 반영된 영국패션의 내재적 특징)

  • Jeong, Hye Yeon;Seo, Seung Hee
    • The Research Journal of the Costume Culture
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    • v.21 no.2
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    • pp.234-245
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    • 2013
  • The modern fashion was developed the basis on the western dress structure and its historical flow was continued until today. Particularly, the Britain has coexised the unique cultural identity in the aristocratic high culture and rebellious tendency of the subculture so it is necessary to the consideration about whole culture in order to grasp the British fashion identity. The purpose of this study was shown the methodological framework of the culture identity research of one country through the background of this formation of culture and process high culture and subculture study by analysis the culture identity in the today's Britain. Also, the purpose of this study that it draws whether the feature of the British fashion shows up as any aspect in this culture identity. The range of this study subdivides and considers with the imperialism, industrial revolution, aristocratism, union nations, and geographical aspect as the island country into the economy, politics, society, and natural characteristic about the Englishness and the notion of British culture then it draws the dichotomy of the British fashion through the culture identity formed in this society cultural background with both sides in the high culture and subculture aspect.

Current Status and Issues of Nuclear Safety Culture

  • Lee, Yong-Hee
    • Journal of the Ergonomics Society of Korea
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    • v.35 no.4
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    • pp.247-261
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    • 2016
  • Objective:The aim of this paper is to discuss the nuclear safety culture in Korea. Background: Recently, Korean nuclear field has met a strong anti-nuclear trend after a few happenings, as well as Fukushima accident, which are turned out to be caused by organizational pitfalls and safety culture defects in general. Method: We review the concepts and constructs of safety culture based on the brief history of the nuclear safety culture that IAEA mainly raised after the Chernobyl accident in 1986. Additionally, we give a comparative discussion on the approach to the safety culture with U.S. Results: Finally, we discuss the three technical domains and propose six selected domestic issues related to the nuclear safety culture for coping with the demanding requests on nuclear safety in Korea. Conclusion and Application: We suggest several recommendations and a research direction in the form of a re-start line for the nuclear safety culture in Korea.

The Research Regarding the Effect which the Duty Satisfaction Causes in Eating out Enterprise Culture (외식기업문화가 직무만족에 미치는 영향에 관한 연구)

  • 임붕영;김형준
    • Culinary science and hospitality research
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    • v.8 no.3
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    • pp.107-122
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    • 2002
  • The analysis result regarding the influence where the enterprise culture goes mad to a duty satisfaction with afterwards is same. First. The enterprise culture factor who causes an effect in the supervision factor of duty satisfaction joint ownership of management idea, appeared with the example by leadership factor. Second. The enterprise culture factor who causes an effect in the promotion factor of duty satisfaction appeared with the all feeling factor of system. Third. The enterprise culture factor who causes an effect in the wages factor of duty satisfaction appeared with the all feeling factor of system. Fourth. The enterprise culture factor who causes an effect in the duty form factor or duty satisfaction clearness or example by leadership and belief, duty affirmation appeared with the factor. Fifth. The enterprise culture factor who causes an effect in the service environment factor of duty satisfaction appeared with the all feeling factor of system. Conclusion the enterprise culture appeared with the fact that it causes an effect duty satisfactorily.

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Factors Indicating Culture Status During Cultivation of Spirulina (Arthrospira) platensis

  • Kim, Choong-Jae;Jung, Yun-Ho;Oh, Hee-Mock
    • Journal of Microbiology
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    • v.45 no.2
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    • pp.122-127
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    • 2007
  • Factors indicating culture status of two Spirulina platensis strains were monitored in a batch mode cultivation for 36 days. Changing mode in all factors showed a common turning point, indicating shift of cell or culture status. Mean biomass productivity was highly sustained until day 22, chlorophyll a concentration peaked on day 22, pH value was > 12 on day 22, coil number was abruptly shortened on day 22, and floating activity was sustained at greater than 79% after day 22, indicating that day 22 is a criterion reflecting phase-transfer in cell physiology in a batch culture system. Many of these changes may have been caused by increased pH, suggesting that pH control is essential for mass production of S. platensis. Fluctuations in floating activity were likely induced by the number of cellular gas vacuoles. Consequently, coil number per trichome and floating activity of S. platensis could readily act as simple indicators for determination of culture status or harvesting time of cells.