• Clinical and Experimental Reproductive Medicine
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• v.26 no.1
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• pp.89-96
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• 1999

Some of the information concerning sexual function in the male diabetes has been focused upon the problems of endocrine or semen parameters. However, the characteristics of acrosome reaction and spermatozoa concentration at the epididymis and vas deferens have scarcely been studied, and the causes of the infertility has not been critically identified. So, we designed to inspect the spermatozoa concentration and the characteristics of acrosome reaction at epididymis and vas deferens of diabetic Wistar rat induced by streptozotocin (STZ, 70 mg/kg, ip). Experimental animal was sacrificed at 3 days and 14 days after the STZ injection. In the diabetes-induced rat, the levels of insulin and glucose had a pattern of inverse proportion. The spermatozoa concentrations in caput and corpus epididymis were significantly decreased in all diabetic condition. In cauda epididymis, however, there was significant decrease in sperm concentration at 14 days onward. In diabetic rat, the spontaneous reaction rate of spermatozoa of cauda and vas deferens were significantly higher than the control group. The ARIC (acrosome reaction to ionophore challenge) value of caudal sperm was 28.7 at control, 22.1 at 3 days, and 8.3 at 14 days. In the present study the spermatozoa concentration was decreased and the spontaneous reaction rate was increased by diabetes. In ARIC-test, it is revealed that the fertility of spermatozoa of 14 days group was lower than control or 3 days group. Diabetes mellitus may be provoke the decreased fertilization rate and subsequent infertility and subsequent infertility.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.2
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• pp.211-216
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• 1997

The present study was carried out to evaluate whether the coculture system of human embryos with Vero cells can improve the quality of embryo or overcome the repetitive implantation failures in order to obtain pregnancy. From January to December 1996, a total 202 cases which patients with the problems of repetitive implantation failures (group I) or those with the poor embryonic quality in their previous cycles (group II) was analysed. The quality of cocultured embryo, pregnancy, on-going and implantation rates between coculture and control groups were compared. Of 93 cases in group I, coculture was performed in 34 cases and conventional IVF for the rest. Of 109 cases in group II, 36 for coculture and 73 for conventional IVF. In group I, pregnancy, on-going and implantation rates in coculture group (14/34 (41.2%), 9/34 (26.5%), 16/81 (19.8%), respectively) were higher than those of control (11/59 (18.6%), 8/59 (13.6%), 12/152 (7.9%), respectively). There is significance in the pregnancy and implantation rates (p=0.028 and p=0.015). In group II, pregnancy, on-going and implantation rates in coculture group (8/36 (22.2%), 5/36 (13.9%), 8/87 (9.2%), respectively) were higher than those of control (5/73 (6.8%), 3/73 (4.1%), 3/158 (1.9%), respectively). Like the result of group I, there is significance in the pregnancy and implantation rates (p=0.028 and p=0.022). Coculture system with Vero cells works well in the groups of the two indications. Although the case of 3 day-coculture was small as 15 cases in group II, 3 day-coculture improved pregnancy rate (4/15 (26.7%)). Therefore, 3 day-coculture with assisted hatching is recommended to the patients with poor embryonic quality. In conclusion, coculture system with Vero cells can be suggested as an effective method which improves pregnancy rate in those who have repetitive implantation failures or whose embryonic quality was poor in their previous cycles.

• Weed & Turfgrass Science
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• v.2 no.1
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• pp.95-99
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• 2013

To investigate the actual condition of production and management of sod, a questionnaire was ask to 57 farmers in major cultivated areas of turf sod in Korea in 2010 and 2011. The results of the turfgrass management situation analysis showed a mixed farming where the ratio of the principal work and the side work progress similarly, and for the landholding type depending on the management scale, the rental ratio was high for a scale over 0.5 ha. Sod production farmers has high-profile in new varieties of turfgrass, integrated management of the fertilization and soil or disease and insect pest however the future of the turfgrass industry does not seem optimistic due to the problems such as market stability or scarcity of worker. Obtaining of information on culture methods depended neighbor's experience (81.8%). Concerning the varieties preference analysis, the 32.1% of farmer answered that the reproductive rate was a key factor for the new variety. Sod production farmers want to receive financial support (28.1%), improvement of distribution structure of sod (26.6%), and spread of good variety turfgrass (23.4%).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.7
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• pp.4632-4640
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• 2015

The purpose of this study was to investigate the education needs related to prenatal care program in married immigrant women and to suggest the meaningful data in developing prenatal care programs. Data collection was conducted in a multi-cultural center, D-city from 2014 Mar to 2015 April and a total of 71 subjects participated in this study. Education needs related to prenatal care programs were investigated using a questionnaire including infertility, human reproductive structures, prenatal educations, vaccinations, health problems managements and so on. Scores in education needs related to prenatal care programs were high in newborn baby safety managements, vaccinations, health care managements, newborn baby normal developments and newborn baby care managements. In addition, education needs related to fertility in the married immigrant women from Vietnam were higher than the married immigrant women from China and others (F=5.53, p<.05). Thus, based on the results of this study, the contents of an educational program needs to focus on newborn baby normal developments and nursing care management for them.

• Reproductive and Developmental Biology
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• v.29 no.1
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• pp.57-62
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• 2005

One of the critical problems to be solved in transgenic animal production is uncontrollable constitutive expression of foreign genes, which usually results in serious physiological disturbances in the transgenic animal. To circumvent this problem, we constructed and tested two retrovirus vectors designed to express the GFP(green fluorescent protein) gene under the control of the tetracycline-inducible promoters. To maximize the GFP gene expression at turn-on state, WPRE(woodchuck hepatitis virus posttranscriptional regulatory element) sequence was introduced into the retrovirus vectors at downstream region of either the GFP gene or the sequence encoding rtTA(reverse tetracycline-controlled transactivator). Transformed cells were cultured in the medium supplemented with or without doxycycline(tetracycline derivative) for 48 hours, and induction efficiency was measured by comparing the GFP gene expression level using fluorometry and western blotting. Higher GFP expression was observed from the vector carrying the WPRE sequence at 3' side of the GFP gene, while tighter expression control(up to 20 fold) was obtained from the vector in which the WPRE sequence was placed at 3' side of rtTA sequence. The resulting tetracycline inducible vector system may be used in transgenic animal production and gene therapy.

• Korean Journal of Health Education and Promotion
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• v.12 no.2
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• pp.73-89
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• 1995

This study was planned to investigate indirectly the effects of study environments of high school students such as family characteristics, availability of sex education, and possibility of exposure to unhealthy sexual environments. For this purpose, the level of knowledge and attitude towards sex of general and industrial high school students were compared because these two groups of students were believed to be different in those study variables. For this study, 600 students (300 male and 300 female students) from a general high school students and 400 (200 male and 200 female) from an industrial high school in one industrial city in Korea were surveyed with a structured questionnaire. The study results were as follows: 1. There were significant differences in selected family condition variables between general and industrial high school students. Proportions of students with single parents, those who live in houses other than their own, and those father's occupations were farming and manufacturing were higher in industrial high school students than in general high school students. 2. Female students wanted to have more knowledge on sex whereas male students were interested in things related to sexual acts in both general and industrial high school students. The largest proportion of students wanted to discuss their sex problems with their friends not with their parents. 3. More students in general high school thought that the sexual intercourse is natural and pretty than those in industrial high school, whereas more industrial high school students insisted on the sexual purity before marriage than general high school students. 4. About 65% of students surveyed agreed to making friends with opposite sex and, in fact, more than half either had experiences or were having friends of opposite sex. More students in industrial high school wanted to choose their marital partners based on advices of their parents than students in general high school. 5. More female students than male and more industrial high school students than general high school students were embarrassed with first wet dream or menstruation and felt guilty about the masturbation. 6. Level of knowledge on reproductive physiology, sex transmitted disease and contraception was higher in general high school students than in industrial high school students. These study results suggest that simple knowledge on sex of the general high school students does not seem to affect positively the students attitude towards sex and the unfavorable living conditions of industrial high school students seem to influence the students to become more sincere and healthy in their attitude towards sex and life. Therefore, more thoughtful consideration in contents and methods should be given when the sex education is provided to the high school students.

• Korea journal of population studies
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• v.29 no.2
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• pp.1-26
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• 2006

This paper examines the interplay among changes in socioeconomic conditions, population policies, and fertility decline during $1960{\sim}2000$ in South Korea. Period-parity-progression-ratios (PPPR) are calculated using 2% samples of Population Census with the implied distribution of completed family size. The total fertility rate based on PPPR declined from 5.21 in 1960 to 2.76 in 1980, and 1.69 in 2000. The family planning programs were successful in meeting the needs of families that wanted to prevent further childbearing after having achieved the desired family size during $1960{\sim}1970$. The 1980s mark two important landmarks: achievement of replacement fertility and emergence of high sex ratio at birth. In the 1990s the "quality and welfare" approach was emphasized. In 2000s, South Korea's fertility is one of the lowest in the world. In response to this, in 2005, the Government adopted a new population policy with the goal of increasing the total fertility level to 1.6 by 2010. The results of this study indicate that proportion of women who would have no children at all throughout their reproductive life span increased substantially from 10% in 1995 to 16% in 2000, with a sharp increase since 1997. Thus, pro-natal programs need to address the problems associated with marriage and having the first child. Towards that, it is important that work and family life become more compatible.

• Development and Reproduction
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• v.13 no.2
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• pp.79-87
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• 2009

Due to its well-known function in parturition and milk ejection, oxytocin (OT) has been considered as a 'female neurohypophyseal hormone'. Recent studies, however, clearly indicate that OT has some local roles in male reproduction via both central and peripheral routes. In experimental rodents, OT is released within distinct brain regions in response to social stimuli, and the brain OT receptor (OTR) mediated actions were strongly involved in the regulation of a variety of male behaviors such as mating-associated behaviors. In particular, OT and/or OTR knockout mice provide important clues about the molecular regulatory mechanism of the socio-sexual behaviors. Several lines of evidence also show that OT is synthesized within rodents testis, epididymis and prostate, and the presence of OTRs in these organs. In rodent testes, OT might have a role in the modulation of steroidogenesis via stimulation of the conversion of testosterone (T) to dihydrotestosterone (DHT) by 5alpha-reductase. Similar effects of OT on this androgen conversion were observed in epididymis and prostate suggesting the OT's modulatory role, such as contractility induction, in these androgen-dependent organs. In this context, further investigations on the OT's role in male CNS and reproductive organs are likely to provide better understanding on the complex socio-sexual behaviors and a platform for development of therapeutics to treat some psychological and/or andrological problems.

• Reproductive and Developmental Biology
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• v.36 no.3
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• pp.213-217
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• 2012

The decreased fertility is frequently thought to be problem of cattle production. However, studies figure out that number of these problems is related to bull factors especially in artificial insemination setting. Therefore, this study was designed to investigate the fertility status of bull by their estimated relative conception rate of cows that were inseminated by frozen semen from Korean proven bulls. Here we use the non-return rate (NRR) to access the bull fertility whereas, the NRR was define as the proportion of bulls that semen were used to inseminate cows and the number of cows that did not return for another service within 60 days. The data from 54,388 artificial inseminations (AI) were analyzed from 88 KPN semen. The NRRs of highest and lowest fertile bull were 83.81 and 51.33%, respectively. And mean NRR was 68.27%. In comparison to previously reported study, our data shows 17.38% higher NRR and the absolute value of difference in 50%>NRR and 50%

• The Journal of Korean Academic Society of Nursing Education
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• v.9 no.2
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• pp.298-309
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• 2003

The educational preparation of nurses has been the focus of considerable debate globally. It is needed the change of the basic nursing education for professional nurse to prepare the trends of the health care needs of clients for coming new generation. And also it is believed that educational preparation for being nurses is to be responsible to clients' needs. The purpose of the study was to develop a baccalaureate program in nursing. This study were implemented through three stages from April 2001, to April 2002.: preparation stage which were consisted of reviewing of the literatures, interviewing the fellow nurses in a variety workplace, and consulting professors who were concerned with the nursing education to identify the present educational problems, and analyzing the contents of seven major courses in nursing education, evaluation stage about a new developed curriculum with the directors of the five nursing schools, and confirmation stage of new developed education programme. The developed education programme was designed as a four year program with 126 credit hours including 39 credit hours of liberal arts, 34 credit hours of supplementary courses, and 53 credit hours of nursing major based on the four basic nursing dimensions of human, health, environment and nursing. Nursing majors were consisted of Nursing Ⅰ(oxygenation), Nursing Ⅱ(nutrition elimination), Nursing Ⅲ(activity rest), Nursing Ⅳ(neurologic- endocrine protection), Nursing Ⅴ(fluids electrolytes/sex reproductive), Nursing Ⅵ(psycho-social), Community Health Nursing, Fundamental Nursing, School Health, and Emergency care. This new nursing programme was focused on the nursing education for prevention and rehabilitation nursing care as well as the acute and chronic nursing care at hospital, on the integrated nursing programme to become effective, and the nursing process to encourage the critical thinking. The new education programme focused on the professional nurses who are responsible the nursing ethics, communication skills, and professional beliefs to suit the future trends in health. And also it will be needed for faculties to manage the integrated curriculum, to analyze the contents of each subject, and to communicate with each other before a new education programme apply to their education programme in future. This research was supported by the Han Kok Medical Science Foundation