• Title/Summary/Keyword: Reproduction experiment

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A Study on the Color Reproduction for Offset Printing using Ecological Ink in the Domestic Printing Environment (국내 인쇄 환경에서 친환경 잉크를 이용한 오프셋 인쇄의 색재현에 관한 연구)

  • Moon, Sung-Hwan;Kim, Sung-Su;Koo, Chul-Whoi;Yoo, Keun-Ryong
    • Journal of the Korean Graphic Arts Communication Society
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    • v.28 no.2
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    • pp.69-85
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    • 2010
  • Currently, environmental contaminants that can cause Aromatic types of hydrocarbons, less than 1% made of Aromatic Free kind of used products, soybean oil products with linseed oil with the products, rice products using a wide range of environmentally ecological ink since 2000 is released quickly. All materials used in printed material, if the green is the best way to print the composite materials in industrial applications, because each process on the print quality and productivity, there can be many differences in this experiment because it accounts for a large proportion in the print general ink in the ink section and the International color standards(ISO2846-1:2006) certified ecological ink were compared. Therefore, in this paper has the ink released from the same company, each common general ink and ecological ink in the same condition which results were focused on whether the emerging international color standard(ISO 2846-1:2006) recognized for environmentally ecological ink printed color reproduction of the actual offset(color reproduction) how conformity to ISO 12647-2 standard color on the basis of the offset would check Color Reproduction. Based on the results of the experiments of this study, given the ecological ink coated paper, uncoated paper both color difference and the gamut of the ISO 12647-2 standard is suitable for ecological ink, the ink's color gamut reproduction, even more than existing international standards, there is no confirmed that the correct color reproduction possible. Using environmentally ecological ink industries is expected to respond to environmental policy.

EVALUATION OF THE COLOR REPREDUCTION ON LCD MONITORS

  • Park, Seung-Ok;Kim, Hong-Suk;Lim, Yong-Jin;Yang, Jae-Youl
    • Proceedings of the Korean Society for Emotion and Sensibility Conference
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    • 2000.04a
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    • pp.407-411
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    • 2000
  • Color reproduction n CRT monitors needs colorimetric characterization concluding two stages with 3x3 matrix and three TRCs (Tone-Reproduction Characteristics). The 3x3 matrix is based on the additivity of RGB emissions, and the TRC represents the non-linearity between the input digital counts and the output luminance. The objective of this project is to evaluate the accuracy of color reproduction on LCD monitors using conventional CRT model(GOG model). For two different manufacturers, colorimetric characterizations of desktop LCDs have been performed. Because the transfer function for LCD is very differ from TRC of CRT, some researchers had reported that GOG model is inadequate for the colorimetric characterization of LCDs. However in our experiment, the degree of accuracy was dependent on the shape of electro-optical transfer functions of LCD. It has been founded that the GOG model could be applied for LCD monitors having monotonically increased Electro-optical transfer function.

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A Study on the Characteristics of Color Reproduction in Digital Printing (디지털인쇄에서 색재현 특성에 관한 연구)

  • Kim, Jae-Hae;Lee, Sung-Hyung;Cho, Ga-Ram;Koo, Chul-Whoi
    • Journal of the Korean Graphic Arts Communication Society
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    • v.24 no.1
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    • pp.23-34
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    • 2006
  • Color reproduction is one of the most important expression factors in digital printing. In this paper, an experiment was done where the characteristics of color reproduction in digital printing. The results could summarized as follows. The printing used device profile showed a color difference of less than printing used default value in digital printing. As a result of weighting color difference, a difference between color gamut of digital original and color gamut of printing, when transforming the RGB color space to CMY color space, a exclusion the gray revision. The solution is to optimize the color transformation by gamut mapping and gray revision.

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Sperm Fertility of Transgenic Boar Harboring hEPO Gene is Decreased

  • Park Chun-Gyu;Kim Sung-Woo;Lee Poong-Yeon;Han Joo-Hee;Lee Hyun-Gi;Byun Sung-June;Yang Boh-Suk;Lee Chang-Hyung;Lee Hoon-Taek;Chang Won-Kyong;Park Jin-Ki
    • Reproductive and Developmental Biology
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    • v.30 no.1
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    • pp.27-34
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    • 2006
  • This study was conducted to compare the reproduction ability of the wild type boar and recombinant human erythropoietin (hEPO) transgenic boar semen. Ejaculated boar semen was analyzed by flow cytometry, Elisa and IVF methods. In experiment 1, flow cytometric analysis showed that the live sperm ratio of transgenic boar sperm significantly lower (P<0.05) than that of wild type boar after incubation at 20, 22, 24 and 26 hr. In experiment 2, the presence and levels of various cytokines (IL-6, IL-10 and $TNF-{\alpha}$) to related animal reproduction in the seminal and blood plasma were examined using specific enzyme immunoassay. There was no significant difference between both groups. In experiment 3, the fertilizing capacity and developmental ability of both boar sperm were compared. The transgenic boar sperm had a significantly low capacity of penetration, sperm-zona binding, embryo development, and blastocyst formation compared to wild type sperm (P<0.05). These results suggest that transgenic boar sperm harboring hEPO gene has low sperm viability than wild type boar, and it is a reason to decrease of fertility and litter size.

A Study on Culture Environments of In Vitro Matured/In Vitro Fertilized Bovine Embryos I. Influence of Somatic Cells, Growth Factors or Culture Media on In Vitro Maturation of Bovine Oocytes (소 체외수정란의 발생배양에 적합한 배양환경 조성 연구 I. 체세포, 성장인자 또는 배양액 종류가 난포란의 체외성숙에 미치는 효과)

  • 이명식;박수봉;박진기;장원경;민관식;백광수;성환후;박용윤
    • Korean Journal of Animal Reproduction
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    • v.22 no.1
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    • pp.95-99
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    • 1998
  • Three experiments were conducted with follicular oocytes, to compare some somatic cells, growth factors and media for in vitro maturation of bovine oocytes. In the first experiment, the type of somatic cells had no effects on in vitro maturation of bovine follicular ooctyes. In the second experiment, oocytes were matured in TCM199 su, pp.emented with growth factors on IVM of bovine follicular oocytes, then all were co-cultured with cumulus cells. The proportion of used oocytes that developed to expanding blastocysts was 22.2%, 20.2%, 17.7%, 22.2%, 24.4% and 20.2% after maturation in TCM199 su, pp.emented with control, insulin, IGF-I, IGF-Ⅱ, FGF and EGF, respectively. In the third experiment, oocytes were matured in BO, Ham's F10 and TCM199, then all were fertilized in BO, and embryos cultured in BO, Ham's F10 and TCM199, respectively. Cleavage rates in BO were 90%, had higher than in Ham's F10(80%) or in TCM199(64%). But production of expanding blastocysts in TCM199(21%) or Ham's F10(20.6%), had higher than in BO(4.6%).

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In vitro Fertilization and Culture of Rabbit Egg (가토난자의 체외수정과 체외배양)

  • 박흠대;이경광;정길생
    • Korean Journal of Animal Reproduction
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    • v.4 no.1
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    • pp.75-82
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    • 1980
  • This experiment was carried out to improve a simple and effective procedure of egg transfer which is considered to be the most useful technique for the improvementand proliferation of domestic animals. Several experiment procedures such as superovulation, surgical recovery of ovulated egg, in vitro capacitation of ejaculated spermatozoa, in vitro fertilization and culture of embryo were conducted. The results obtained in this experiment were summarized as follows: 1. In rabbits treated with PMS in combination with estradiol and HCG, 10 to 43 eggs were obtained in one rabbit and the average ovulation number of 20 rabbits was 21 eggs. 2. Six to 24 eggs (average 13 eggs) were recovered from the removed oviducts by the methods of flushing technique and the average recovery rate of 20 rabbits was 61.9 percent. 3. Most rabbit spermatozoa ejaculated by artificial vagina were capacitated in vitro by the culture of the spermatozoa with PBI medium at 37$^{\circ}C$ for 4 hours after removal of seminal plasma. 4. Normal fetilization following in vitro fetilization was observed in 88 (42.7%) of 206 eggs. 5. The number of eggs developed to 2-, 4-, and 8-cell stage following in vitro culture with PBI medium at 37$^{\circ}C$ was 26 (70.3%), 20 (54.1%) and 17 (45.9%) of 37 eggs used for in vitro culture.

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Studies on the Farrowing Inducction of Sow with Prostaglandin $F_2\alpha$ II. Health and Growth of Piglets Artificially borne by the Prostaglandin F2$\alpha$ Administration (Prostaglandin $F_2\alpha$ 투여에 의한 돼지의 분만유기에 관한 연구 II. 유기분만자돈의 건강과 발육)

  • 연정웅;정길생
    • Korean Journal of Animal Reproduction
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    • v.3 no.2
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    • pp.50-56
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    • 1979
  • This experiment was conducted to clarity the possibility of practical use of farrowing inductionin sow by the administration of prostaglandin F2$\alpha$. For this experiment, total 320 heads of pregnant sow and its piglets were used. The reproductive characteristics of artificially farrowed sow and, health and growth state of piglets were estimated. The results obtained in this experiment were summarized as following: 1. No significant difference were observed between naturally and artifically farrowed sow in several aspects such as the rate of dystocia, length of farrowing, farrowing intervals from piglet to piglet. 2. significant (P<0.05) differences were observed between naturally and artificially farrowed sow in intervals from weaning to estrus. However, there were no significant differences among those of 5, 7.5 and 10mg treated group. 3. There were no differences in number of stillbirth, immature birth and alive piglets at 3 weeks age per litter were observed. 4. Similar birth weight, weaning weight, daily gain and rearing rate of piglets were obtained from both naturally and artificially farrowing.

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In Vivo Transfer of Foreign DNA into Primordial Germ Cells (PGCs) of Chicken Embryos

  • Eguma, K.;Soh, T.;Hattori, M.;Fujihara, N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.4
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    • pp.520-524
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    • 1999
  • The present experiments were designed to examine whether exogenous DNA injected into the germinal crescent region (GCR) of early stage of developing embryos, which is considered to be the main place from which PGCs originate, can be transferred to recipient chicken embryos. In this experiment, Miw Z (DNA) dissolved in the transfection reagent (TR: Boehringer, Germany) was introduced into the GCR of donor embryos at stage 3-5 or 9-11, followed by continued incubation until the stage 13-15 of embryonic development. The PGCs collected from the embryonic blood vessels were examined for the incorporation of the injected DNA into the PGCs by the methods of X-gal staining and PCR analysis. As the results, the foreign DNA was successfully incorporated into the PGCS, leading to their transfer to the gonadal tissues. The present results, therefore, suggest that the early stage (3-5 or 9-11) of chicken embryonic development would be more successful than stage 13-15 in transferring exogenous genes to the recipient embryos, leading to the possibility of producing transgenic chicken medianting the PGCS.

신개발 국산 부가중합 실리콘 인상재의 젖음성과 기포발생에 관한 비교 연구

  • 조리라;정경호;김경남
    • The Journal of the Korean dental association
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    • v.41 no.1 s.404
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    • pp.35-41
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    • 2003
  • Wettability of addition silicone impression material is very important property for making an accurate restoration. This study examined theimpression quality in clinical condition and the wettability of impression material. Three commercially available addition silicone impression material (Imprint; 3M, USA, Examix; GC, Japan, Perfect; Handae, Korea) were studied. A total of 90 putty/wash impressions of semi-dried premolars and wet molar teeth were examined for void production in impression body. The percentae of the sulcus reproduction ability of each material was calculated from the sulcus depths of cross-sectioned casts from the impressions with stereomicroscope. Three impression materials were used to produce die stone casts from vcid entrapment die. Voids in the stone casts were counted with the stereomicroscpe. From the experiment, the following results were obtained: 1. In direct observation, Imprint showed greatest numbers of void in impression body (P<.001).However, correlations were not found between sulcus reproduction and void production. 2. Sulcus reproduction ability of additional silicone impression material was diminished in order of Imprint, Examix, Perfect. The significant difference was found between Imprint and other material (P<.001). 3. In void entrapment laboratory test, void productions was diminished in order of Examix, Imprint, Perfect. All voids in casts were less in delayed poured cast than immediately poured cast. 4. Especially, the stone pouring time of Perfect impression material should be delayed.

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Effect of Antioxidants and Co-culture System on the Development of Bovine Embryos Derived from In Vitro Fertilization I. Effect of Antioxidants and Amino Acids on the Development of Bovine IVM/IVF Embryos (항산화제 첨가와 체세포 공동배양이 소 체외수정란의 체외발육에 미치는 영향 I. 항산화제 첨가가 소 체외수정란의 체외발육에 미치는 효과)

  • 양부근;황환섭;박동헌;정희태;박춘근;김종복;김정익
    • Korean Journal of Animal Reproduction
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    • v.20 no.2
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    • pp.163-170
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    • 1996
  • The effect of several potential antioxidants and amino acids were examined as a means of increasing the development of in vitro matured and in vitro fertilized oocytes into morulae or blastocysts. Bovine embryos developed to the 2~8 cell stage after in vitro fertilization were cultured for 5 to 6 days at 39$^{\circ}C$ in CR1aa containing varing concentraton of the antioxidants and amino acid in a gas phase consisting of 5% CO2, high humidified air. At 5~6 days, embryo developments were reduced, and embryos were fixed and stained with Hochest 33342 DNA stain to facilitate counting of cells. In experiment 1, the proportion of embryos developed to morulae and blastocysts in CR1aa containing 1mM, 2.5mM taurine (22.6% and 20.4%) was slightly higher than those of 0, 5 and 10mM Taurine (5.7, 5.7 and 3.9%, P<0.05). In experiment 2, addition of glutathione did not improve blastocyst development (P>0.05). In experiment 3, concentations of superoxide dismutase(SOD) ranging from 300 to 1,000 U did not affect the propotion of embryos developing into blastocysts (P>0.05). In experiment 4, addition of 250 U catalase(38.5%) was slighty higher than those of 0, 500 and 1,000U. In experiment 5, the proportion of embryo developed beyond morula stage in CR1aa with taurine plus EDTA was slighty higher than other treatments(15.7, 26.0 and 29.2%), there were no significantly increases in cell number among treatments(P>0.05). These results are indicating that antioxidants and amino acids can increase the proportion of embryos that develop into morulae and blastocysts, but did not increas in cell number of blastocysts.

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