• Asian-Australasian Journal of Animal Sciences
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• v.22 no.6
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• pp.893-899
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• 2009

Few studies have reported the existence of imprinted genes in cattle compared to the human and mouse. Genomic imprinting is expressed in monoallelic form and it depends on a single parent-specific form of the allele. Comparative analysis of mammals other than the human is a valuable tool for explaining the genomic basis of imprinted genes. In this study, we investigated 34 common imprinted genes in the human and mouse as well as 35 known non-imprinted genes in the human. We found short interspersed nuclear elements (SINEs), long interspersed nuclear elements (LINEs), and long terminal repeats (LTRs) in imprinted (human and mouse) and control (cattle) genes. Pair-wise comparisons for the three species were conducted using SINEs, LINEs, and LTRs. We also calculated 95% confidence intervals of frequencies of repetitive sequences for the three species. As a result, most genes had a similar interval between species. We found 11 genes with conserved SINEs, LINEs, and LTRs in the human, mouse, and cattle. In conclusion, eleven genes (CALCR, Grb10, HTR2A, KCNK9, Kcnq1, MEST, OSBPL5, PPP1R9A, Sgce, SLC22A18, and UBE3A) were identified as candidate imprinted genes in cattle.

• Genomics & Informatics
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• v.12 no.4
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• pp.261-267
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• 2014

MicroRNAs (miRNAs) are known for their role in mRNA silencing via interference pathways. Repetitive elements (REs) share several characteristics with endogenous precursor miRNAs. In this study, 406 previously identified and 1,494 novel RE-derived miRNAs were sorted from the GENCODE v.19 database using the RepeatMasker program. They were divided into six major types, based on their genomic structure. More novel RE-derived miRNAs were confirmed than identified as RE-derived miRNAs. In conclusion, many miRNAs have not yet been identified, most of which are derived from REs.

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.89.2-89
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• 1999

No Abstract, See Full Text

• Journal of the Society of Naval Architects of Korea
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• v.40 no.5
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• pp.1-9
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• 2003

To predict the powering performance of full scale ships from the towing tank tests, resistance, propeller open water and self-propulsion tests are conducted. Model tests inevitably include the experimental error defined as the sum of two types of uncertainties, bias and precision errors. The induced errors in each element of model test are propagated through various routes and correlated with one another. The correlation coefficients are very important in the uncertainty analysis. The coefficient gives a direction(increase or decrease) for a value of error in individual elements. If the coefficient is not used accurately, the error bounds of the individual elements are overestimated or underestimated. In this study, the new methodology is applied to the uncertainty analysis of HMRI's towing tank tests, thus error bounds of each element is suggested and verified by several repetitive experiments.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1995.06b
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• pp.1-19
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• 1995

The plasmid pJEL 101 contains a highly repetitive element from the genome of Xanthomonas oryae pv. oryzae that has properties of an insertional element. The insertional nature of the element, hereto referred to as IS203, was confirmed by molecular analyses of the element and three related elements that were isolated from X. oryzae. The related sequences were isolated on the basis of transposition to the transposon-trapping vector pL3SAC and hybridization with pJEL101. The trapped elements (IS203a, IS203b, and IS203c) were each composed of 1,055 base pairs with 25 base terminal inverted repeats. The elements caused a three base pair target site duplication at the site of insertion in the sacRB gene. The sequence of pJEL 101 has 96% base pair identity with IS203a and 99% identity with IS203a and IS203c but lacks three nucleotides of the consensus left terminal repeat. IS203b has the same DNA sequences as IS203c but is inserted ito the sacRB gene in the opposite orientation. The longest open reading frame of IS203a could code for a protein of 318 amino acids and molecular weight of 37, 151. A search of the Genbank database revealed that IS203 has 51% identity with 909 nucleotides of IS4551 from Escherichia coli. The predicted protein of ORF1 has 40% and 30% amino acid identity to the ORF1 of Tn4551 and the transposase of IS30, respectively.

• Journal of Microbiology and Biotechnology
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• v.22 no.1
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• pp.13-24
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• 2012

The genetic diversity of plant growth-promoting rhizobacterial (PGPR) fluorescent pseudomonads associated with the sugarcane (Saccharum officinarum L.) rhizosphere was analyzed. Selected isolates were screened for plant growthpromoting properties including production of indole acetic acid, phosphate solubilization, denitrification ability, and production of antifungal metabolites. Furthermore, 16S rDNA sequence analysis was performed to identify and differentiate these isolates. Based on 16S rDNA sequence similarity, the isolates were designated as Pseudomonas plecoglossicida, P. fluorescens, P. libaniensis, and P. aeruginosa. Differentiation of isolates belonging to the same group was achieved through different genomic DNA fingerprinting techniques, including randomly amplified polymorphic DNA (RAPD), amplified ribosomal DNA restriction analysis (ARDRA), repetitive extragenic palindromic (REP), enterobacterial repetitive intergenic consensus (ERIC), and bacterial repetitive BOX elements (BOX) analyses. The genetic diversity observed among the isolates and rep-PCR-generated fingerprinting patterns revealed that PGPR fluorescent pseudomonads are associated with the rhizosphere of sugarcane and that P. plecoglossicida is a dominant species. The knowledge obtained herein regarding the genetic and functional diversity of fluorescent pseudomonads associated with the sugarcane rhizosphere is useful for understanding their ecological role and potential utilization in sustainable agriculture.

• Korean Journal of Food Science and Technology
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• v.37 no.4
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• pp.611-617
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• 2005

Dispersed repetitive DNA elements in genomes of microorganisms differ among and within species. Because distances between repetitive sequences vary depending on bacterial strains, genomic fingerprinting with interspersed repetitive sequence-based probes can be used to distinguish unrelated organisms. Among well-known bacterial repetitive sequences, Repetitive Extragenic Palindromic (REP) sequence has been used to identify environmental bacterial species and strains. We applied REP-PCR to detect and differentiate four major Gram-negative food-borne bacterial pathogens, E. coli, Salmonella, Shigella, and Vibrio. Target DNA fragments of these pathogens were amplified by REP-PCR method. PCR-generated DNA fragments were separated on 1.5% agarose gel. Dendrograms for PCR products of each strain were constructed using photo-documentation system. REP-PCR reactions with primer pairs REP1R-I and REP2-I revealed distinct REP-PCR-derived genomic fingerprinting patterns from E. coli, Salmonella, Shigella, and Vibrio. REP-PCR method provided clear distinctions among different bacterial species containing REP-repetitive elements and can be widely used for typing food-borne Gram-negative strains. Results showed established REP-PCR reaction conditions and generated dendrograms could be used with other supplementary genotyping or phenotyping methods to identify isolates from outbreak and to estimate relative degrees of genetic similarities among isolates from different outbreaks to determine whether they are clonally related.

• Structural Engineering and Mechanics
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• v.4 no.5
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• pp.553-568
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• 1996

Finite element stiffness matrix methods are presented for finding natural frequencies (or buckling loads) and modes of repetitive structures. The usual approximate finite element formulations are included, but more relevantly they also permit the use of 'exact finite elements', which account for distributed mass exactly by solving appropriate differential equations. A transcendental eigenvalue problem results, for which all the natural frequencies are found with certainty. The calculations are performed for a single repeating portion of a rotationally or linearly (in one, two or three directions) repetitive structure. The emphasis is on rotational periodicity, for which principal advantages include: any repeating portions can be connected together, not just adjacent ones; nodes can lie on, and members along, the axis of rotational periodicity; complex arithmetic is used for brevity of presentation and speed of computation; two types of rotationally periodic substructures can be used in a multi-level manner; multi-level non-periodic substructuring is permitted within the repeating portions of parent rotationally periodic structures or substructures and; all the substructuring is exact, i.e., the same answers are obtained whether or not substructuring is used. Numerical results are given for a rotationally periodic structure by using exact finite elements and two levels of rotationally periodic substructures. The solution time is about 500 times faster than if none of the rotational periodicity had been used. The solution time would have been about ten times faster still if the software used had included all the substructuring features presented.

• Korean Institute of Interior Design Journal
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• v.26 no.3
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• pp.25-33
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• 2017

The purpose of this study is to analyze the characteristics of interior space in his design hotel through the analysis of Tony Chi 's work and to present the direction of the design direction of the interior space of the design hotel in the future based on the results. The results are summarized as follows. First, the characteristics of the interior space of Tony Chi are divided into visual balance, repetitive rhythm, symbolic emphasis, exaggerated scale, design harmony, and physical borderlessness. Second, the spatial composition through visual balance was consistently shown in all cases, mainly by arranging elements such as furniture and lighting symmetrically. Third, the spatial composition through repetitive rhythm did not show its characteristics at the beginning of the works, but gradually appeared as repetitive rhythm expression using bold pattern. Fourth, Toni Chi constantly expressed the space composition through symbolic emphasis during the planning of the design hotel. Fifth, the exaggerated scale was mainly used to express the architectural detail vertically according to the height of the ceiling to express a grand space and appear in many works. Sixth, Toni Chi consistently expressed the composition of space through the harmony of design of oriental and western cultures, classical and modern designs in all cases. Seventh. In order to provide curiosity induction and aesthetic experience, spatial composition through physical borderlessness was used.

• Geomechanics and Engineering
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• v.19 no.1
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• pp.79-91
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• 2019

Renewed interest in the long-term pile foundations has been driven by the increase in offshore wind turbine installation to generate renewable energy. A monopile subjected to repetitive loads experiences an evolution of displacements, pile rotation, and stress redistribution along the embedded portion of the pile. However, it is not fully understood how the embedded pile interacts with the surrounding soil elements based on different pile geometries. This study investigates the long-term soil response around offshore monopiles using finite element method. The semi-empirical numerical approach is adopted to account for the fundamental features of volumetric strain (terminal void ratio) and shear strain (shakedown and ratcheting), the strain accumulation rate, and stress obliquity. The model is tested with different strain boundary conditions and stress obliquity by relaxing four model parameters. The parametric study includes pile diameter, embedded length, and moment arm distance from the surface. Numerical results indicate that different pile geometries produce a distinct evolution of lateral displacement and stress. In particular, the repetitive lateral load increases the global lateral load resistance. Further analysis provides insight into the propagation of the shear localization from the pile tip to the ground surface.