• Geotechnical Engineering
• /
• v.14 no.3
• /
• pp.47-62
• /
• 1998

The disturbed state concept (DSC) proposed here is based on the idea that a deforming material element can be treated as a mixture of two constituent parts in the relative intact (Rl) and fully adjusted (FA) states, referred to as reference states. Based on this idea, DSC provides a unified constitutive model for the characteristics of saturated sands under cyclic loading. The model parameters for saturated sands are evaluated by using data from truly triaxial test device, The laboratory test results are also used for the verification of D SC model. In general, the model predictions are found to provide satisfactory correlation with the test results. From the results of this research, it can be stated that the DSC model is capable of characterizing the cyclic behavior of saturated sands under dynamic loading.

• Journal of the Korean Geotechnical Society
• /
• v.16 no.3
• /
• pp.5-13
• /
• 2000

In this study, the Disturbed State Concept (DSC) constitutive model is calibrated and modified for steel-sand interface by using the HiS S model for relative intact (Rl) state and the critical state model for the fuBy adjusted (FA) part in the material. The general formulation for implementation is developed. Then, the DSC model with modification for interface is implemented in finite element program based on the generalized Biot's theory. The interface test under one-way monotonic and two-way cyclic loading were numerically simulated using the finite element program modified in this study. The DSC predictions show improved agreement with the observed results from laboratory test. Overall, the computer procedure with the DSC allows relatively improved simulation ofthe soil-structure interaction problems.oblems.

• Tunnel and Underground Space
• /
• v.8 no.3
• /
• pp.200-208
• /
• 1998

This research is intended to investigate the behavior of the jointed rock under various loading conditions: static or dynamic load. The distributed state concept (DSC) is based on the idea that the response of the joint can be related to and expressed as the response of the reference states : relative intact (RI) and fully adjusted (FA) states. In the DSC, an initially RI joint modifies continuously through a process of natural self-adjustment, and a part of it approaches the FA state at randomly disturbed locations in the joint areas. In this study, based on the DSC concept, RI state, FA state, and disturbance function (D) are defined for characterizing the behavior of rock joint. From the results of this research, it can be stated that DSC model is capable of capturing the physical behavior of jointed rock such as softening and hardening and considering the size of joint and roughness of joint surface.

• Mass Spectrometry Letters
• /
• v.12 no.3
• /
• pp.85-92
• /
• 2021

The therapeutic antibody drug market has experienced explosive growth as mAbs become the main therapeutic modality for a variety of diseases. Characterization of glycosylation that directly affects the efficacy and safety of therapeutic monoclonal antibodies (mAbs) is critical for therapeutics development, bioprocess system optimization, lot release, and comparability evaluation. The LC/MS approach has been widely used to structurally characterize mAbs, and recently attempts have been made to obtain comprehensive information on the primary structure and post-translational modifications (PTMs) of mAbs through intact protein analysis. In this study, we performed state-of-the-art LC/MS based intact protein analysis to readily identify and characterize glycoforms of various mAbs. Different glycoforms of mAbs produced in different expression cell lines including CHO, SP2/0 and HEK cells were monitored and compared. In addition, the comparability of protein molecular weight, glycoform pattern, and relative abundances of glycoforms between the commercialized trastuzumab biosimilar and the original product was determined in detail using the given platform. Intact mAb analysis allowed us to gain insight into the overall mAb structure, including the complexity and diversity of glycosylation. Furthermore, our analytical platform with high reproducibility is expected to be widely used for biopharmaceutical characterization required at all stages of drug development and manufacturing.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.12
• /
• pp.2034-2042
• /
• 2015

A one-pot process of enzymatic synthesis of deoxythymidine-5'-triphosphate (5'-dTTP) employing whole cells of recombinant Escherichia coli coexpressing thymidylate kinase (TMKase) and acetate kinase (ACKase) was developed. Genes tmk and ack from E. coli were cloned and inserted into pET28a(+), and then transduced into E. coli BL21 (DE3) to form recombinant strain pTA in which TMKase and ACKase were simultaneously overexpressed. It was found that the relative residual specific activities of TMKase and ACKase, in pTA pretreated with 20 mM ethylene diamine tetraacetic acid (EDTA) at 25℃ for 30 min, were 94% and 96%, respectively. The yield of 5'-dTTP reached above 94% from 5 mM deoxythymidine 5'-monophosphate (5'-dTMP) and 15 mM acetyl phosphate catalyzed with intact cells of pTA pretreated with EDTA. The process was so effective that only 0.125 mM adenosine-5'-triphosphate was sufficient to deliver the phosphate group from acetyl phosphate to dTMP and dTDP.

• Proceedings of the National Institute of Ecology of the Republic of Korea
• /
• v.5 no.1
• /
• pp.10-20
• /
• 2024

The study investigated species diversity, relative abundance, and decline of flying insects and plants within a fragmented forest in the Federal University of Technology Akure (FUTA), Ondo State, Nigeria. It is known that habitat fragmentation can reduce biodiversity. Thus, it is important to perform comprehensive assessments to understand implications of the habitat fragmentation for flora and fauna. Species richness and abundance of flying insects and plants across fragmented forest patches were quantified using field surveys and taxonomic identification. This study revealed shifts in species diversity, with fragmented areas exhibiting reduced biodiversity compared to contiguous forest ecosystems. Flying insects crucial for ecosystem functioning and pollination services demonstrated decreased species richness and relative abundance within fragmented habitats. This decline was attributed to habitat loss, altered microclimates, and limited movement pathways known to hinder insect dispersal. Similarly, plant species richness and abundance showed decline in fragmented forest due to disrupted mutualistic interactions with pollinators, altered nutrient cycling, and increased competition among plant species. This study underscores the importance of maintaining intact forest habitats to sustain healthy ecosystems and preserve biodiversity. Effective conservation strategies should focus on habitat connectivity, reforestation efforts, and protection of essential ecological corridors to mitigate effects of fragmentation. In conclusion, this investigation provides empirical evidence for effects of habitat fragmentation on flying insects and plants in a forest ecosystem in FUTA Akure, Nigeria. Findings emphasize an urgency of adopting conservation measures to safeguard these invaluable components of biodiversity and ecosystem stability in the face of ongoing habitat loss and fragmentation.

• ALGAE
• /
• v.35 no.3
• /
• pp.237-252
• /
• 2020

Temperate rocky reefs dominated by the giant kelp, Macrocystis pyrifera, support diverse assemblages of benthic macroalgae that provide a suite of ecosystem services, including high rates of primary production in aquatic ecosystems. These forests and the benthic macroalgae that inhabit them are facing both short-term losses and long-term declines throughout much of their range in the eastern Pacific Ocean. Here, we quantified patterns of benthic macroalgal biomass and irradiance on rocky reefs that had intact kelp forests and nearby reefs where the benthic macroalgae had been lost due to deforestation at three sites along the California, USA and Baja California, MEX coasts during the springs and summers of 2017 and 2018. We then modeled how the loss of macroalgae from these reefs impacted net benthic productivity using species-specific, mass-dependent rates of photosynthesis and respiration that we measured in the laboratory. Our results show that the macroalgal assemblages at these sites were dominated by a few species of stipitate kelps and fleshy red algae whose relative abundances were spatially and temporally variable, and which exhibited variable rates of photosynthesis and respiration. Together, our model estimates that the dominant macroalgae on these reefs contribute 15 to 4,300 mg C m^-2 d^-1 to net benthic primary production, and that this is driven primarily by a few dominant taxa that have large biomasses and high rates of photosynthesis and / or respiration. Consequently, we propose that the loss of these macroalgae results in the loss of an important contribution to primary production and overall ecosystem function.

• Journal of the Korean Geotechnical Society
• /
• v.19 no.5
• /
• pp.223-232
• /
• 2003

In this study, a constitutive model called the disturbed state concept (DSC) was modified to be applied to the interface shear stress-displacement relationship between geosynthetics. The DSC model is comprised of two reference states, namely the relative intact (RI) and the fully adjusted (FA) state, and one function, namely the disturbance function. This model is a unified approach and can allow for various models as an RI state such as elastic-perfectly plastic model, hierarchical model, and so on. In addition, by using this model, the elastic and plastic displacements can be considered simultaneously. Comparisons between the measured data and predicted results through the parameters determined from four sets of large direct shear tests showed good agreements with each other, especially for the smooth geomembrane-involved interface. Although there are slight differences at peak shear strength for textured geomembrane-involved interface, this model can still be useful to predict the position of displacement at peak strength and the large displacement (or residual) shear strength.

• Journal of the Korean Geotechnical Society
• /
• v.19 no.3
• /
• pp.93-104
• /
• 2003

The Disturbed State Concept (DSC) model, with simplified unloading/reloading formulation, is implemented in a nonlinear dynamic finite element program fur porous media named DSC_DYN2D. In this research, the DSC constitutive model is utilized using the HiSS model for relative intact (RI) part and the critical state model for the fully adjusted (FA) part in the material. The general formulation for implementation is developed. The cyclic loading tests from the field load test data on a pile segment were numerically simulated using the finite element program DSC_DYN2D and compared with field measurements and those from the previous analysis with the HiSS model. The DSC predictions show improved agreement with the field behavior of the pile compared to those from the HiSS model. Overall, the computer procedure with the DSC model allows improved and realistic simulation of the complex dynamic soil-structure interaction problems.

• Journal of Biomedical Engineering Research
• /
• v.14 no.2
• /
• pp.155-162
• /
• 1993

The human's biomechanical structure keeps an optimal state by adapting the original biomechanical structure according to a change in the physical environment. This phenomenon is believed to be the main cause of loosening of the total hip replacement which is used widely in these days. In this study the bone density change due to artificial hip joint, which is generally believed as bone-remodeling, was investigated by the finite element method. For this, 2-D FEM models with 4 nodal point elements were constructed for intact and implanted cases. The density was calculated by comparing the relative amounts of effective stress for these two cases. In this way, calculated new density values were used in the next step as input values and this procedure repeated until convergence was obtained. Severe density change was detected at the femoral cortex of the proximal-medial side as expected. Moreover, following surprising result was found from this analysis. Titanium alloy prosthesis showed less density change compared to stainless steel prosthesis at earlier stage, however, almost same amount of the density change was detected at final stage. It was also found that other design parameters could not significantly affect its density change.