• Journal of Ginseng Research
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• v.44 no.2
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• pp.341-349
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• 2020

Background: The replicative senescence of human dermal fibroblasts (HDFs) is accompanied by growth arrest. In our previous study, the treatment of senescent HDFs with Rg3(S) lowered the intrinsic reactive oxygen species (ROS) levels and reversed cellular senescence by inducing peroxiredoxin-3, an antioxidant enzyme. However, the signaling pathways involved in Rg3(S)-induced senescence reversal in HDFs and the relatedness of the stereoisomer Rg3(R) in corresponding signaling pathways are not known yet. Methods: We performed senescence-associated β-galactosidase and cell cycle assays in Rg3(S)-treated senescent HDFs. The levels of ROS, adenosine triphosphate (ATP), and cyclic adenosine monophosphate (cAMP) as well as the mitochondrial DNA copy number, nicotinamide adenine dinucleotide (NAD)⁺/1,4-dihydronicotinamide adenine dinucleotide (NADH) ratio, and NAD-dependent sirtuins expression were measured and compared among young, old, and Rg3(S)-pretreated old HDFs. Major signaling pathways of phosphatidylinositol 3-kinase/Akt, 5' adenosine monophosphate-activated protein kinase (AMPK), and sirtuin 1/3, including cell cycle regulatory proteins, were examined by immunoblot analysis. Results: Ginsenoside Rg3(S) reversed the replicative senescence of HDFs by restoring the ATP level and NAD⁺/NADH ratio in downregulated senescent HDFs. Rg3(S) recovered directly the cellular levels of ROS and the NAD⁺/NADH ratio in young HDFs inactivated by rotenone. Rg3(S) mainly downregulated phosphatidylinositol 3-kinase/Akt through the inhibition of mTOR by cell cycle regulators like p53/p21 in senescent HDFs, whereas Rg3(R) did not alter the corresponding signaling pathways. Rg3(S)-activated sirtuin 3/PGC1α to stimulate mitochondrial biogenesis. Conclusion: Cellular molecular analysis suggests that Rg3(S) specifically reverses the replicative senescence of HDFs by modulating Akt-mTOR-sirtuin signaling to promote the biogenesis of mitochondria.

• Korean Journal of Oriental Medicine
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• v.12 no.1
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• pp.23-38
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• 2006

The taxonomic list of specific features in external and internal shape and the pattern analysis of Aconitum carmichalei $D_{EBX}$ as the original plant of Aconiti Lateralis Radix Preparata and Aconitum cliiare Dc as the original plant of Aconiti Ciliare Tuber are as follows. 1. Aconitum carmichalei $D_{EBX}$ has tri-palmately parted leaves, petiole in lower leaves, and its ovary has short hair. Whereas Aconitum cliare Dc has $3{\sim}4$ parted leaves, long petiole, and its ovary has not hair. 2. Aconitum carmichalei $D_{EBX}$ has cylinder shape is relatively small in length and diameter, is greyish brown blacky brown in outer surface, greyish $white{\sim}dark$ gray in section. 3. According to the collection place, there is a remarkable difference in the physical shape of herbal states. Aconiti Lateralis Radix Preparate(medicated in Korea) is more transparent blacky brown color than Aconiti Lateralis Fadix Preparata(medicated in Chian). Also Black Aconi Radix(墨附片) has exodermis and White Aconi Radix(白附片) has not. 4. The internal characteristics entirely correspond to in internal shape described in the literatures, Only it is possible to discriminate between black Aconi Radix(墨附片) and White Aconi Radix(白附片) by the existence of cork layer. The classification between Aconiti Lateralis Radix Preparata and Aconiti Ciliare Tuber makes entirely Tuber makes entirely remarkable difference in the physical shape of cambium layer Namely, in shape of cambium layer the kinds of Aconiti lateralis Radix Prepala has horn-like shape and the kinds of Aconiti Ciliare Tuber has circle-like shape. 5. In the peak of examination substance in comparison to Rt of the index material diterpene alkaloid mesaconitine, aconitine, hypaconitine chromatogram Aconiti Ciliare Tuber is higher than in Aconiti Lateralis Radix Preparata This explain that the component changes after the process of medicine. 6. In the Content of mesaconitine, aconitine and hypaconitime Aconiti Ciliare Tuber is higher than Aconiti Lateralis Radix Preparata. 7. In Aconiti Lateralis Radix Preparata, aconitine, hypaconitine and mesaconitine each appears in Rf 0.46, 0.54, 0.32. But except Aconiti Ciliare Tuber the band does not appear. For the future, such results will be used as the basic source of additional research, and a far-reaching comparative study is needed to distinguish between many kinds of same genus-degree of relatedness.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.2
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• pp.287-293
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• 2017

The purpose of the study was to analyze the effects of work experience during higher education on employment. In particular, the study analyzed the difference in employment and permanent employment according to work experience or internship. In addition, the effects of the relatedness of college major and work experience as well as internship on college graduates' employment or permanent employment were investigated. The data came from the 2013 Graduates Occupational Mobility Survey (GOMS). The study covered the chi-square test and logistic regression analysis. The study found that there was a significant difference in the employment and permanent employment among the groups classified by work experience or internship. Internship had a positive effect on college graduates' permanent employment. Based on the study results, this study suggests that policies for internship during higher education need to be enhanced. The match between employment and the field of study in college needs to be increased. Interventions and policies for the establishment of competency-based employment should be improved.

• Horticultural Science & Technology
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• v.33 no.5
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• pp.737-750
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• 2015

This study was carried out to construct a DNA profile database for 102 watermelon cultivars through the comparison of polymorphism level and genetic relatedness using genomic microsatellite (gMS) and expressed sequence tag (EST)-microsatellite (eMS) markers. Sixteen gMS and 10 eMS primers showed hyper-variability and were able to represent the genetic variation within 102 watermelon cultivars. With gMS markers, an average of 3.63 alleles per marker were detected with a polymorphism information content (PIC) value of 0.479, whereas with eMS markers, the average number of alleles per marker was 2.50 and the PIC value was 0.425, indicating that eMS detects a lower polymorphism level compared to gMS. Cluster analysis and Jaccard's genetic distance coefficients using the unweighted pair group method with arithmetic average (UPGMA) based on the gMS, eMS, and combined data sets showed that 102 commercial watermelon cultivars could be categorized into 6 to 8 major groups corresponding to phenotypic traits. Moreover, this method was sufficient to identify 78 out of 102 cultivars. Correlation analysis with Mantel tests for those clusters using 3 data sets showed high correlation ($r{\geq}0.80$). Therefore, the microsatellite markers used in this study may serve as a useful tool for germplasm evaluation, genetic purity assessment, and fingerprinting of watermelon cultivars.

• Journal of Veterinary Science
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• v.22 no.5
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• pp.68.1-68.15
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• 2021

Background: Colistin and carbapenem-resistant bacteria have emerged and become a serious public health concern, but their epidemiological data is still limited. Objectives: This study examined colistin and carbapenem resistance in Escherichia coli and Salmonella from pigs, pig carcasses, and pork in Thailand, Lao PDR, and Cambodia border provinces. Methods: The phenotypic and genotypic resistance to colistin and meropenem was determined in E. coli and Salmonella obtained from pigs, pig carcasses, and pork (n = 1,619). A conjugative experiment was performed in all isolates carrying the mcr gene (s) (n = 68). The plasmid replicon type was determined in the isolates carrying a conjugative plasmid with mcr by PCR-based replicon typing (n = 7). The genetic relatedness of mcr-positive Salmonella (n = 11) was investigated by multi-locus sequence typing. Results: Colistin resistance was more common in E. coli (8%) than Salmonella (1%). The highest resistance rate was found in E. coli (17.8%) and Salmonella (1.7%) from Cambodia. Colistin-resistance genes, mcr-1, mcr-3, and mcr-5, were identified, of which mcr-1 and mcr-3 were predominant in E. coli (5.8%) and Salmonella (1.7%), respectively. The mcr-5 gene was observed in E. coli from pork in Cambodia. Two colistin-susceptible pig isolates from Thailand carried both mcr-1 and mcr-3. Seven E. coli and Salmonella isolates contained mcr-1 or mcr-3 associated with the IncF and IncI plasmids. The mcr-positive Salmonella from Thailand and Cambodia were categorized into two clusters with 94%-97% similarity. None of these clusters was meropenem resistant. Conclusions: Colistin-resistant E. coli and Salmonella were distributed in pigs, pig carcasses, and pork in the border areas. Undivided-One Health collaboration is needed to address the issue.

• Korean Journal of Heritage: History & Science
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• v.48 no.2
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• pp.4-29
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• 2015

This article considered generally gradational change aspect, character of community for the tombs of the Three kingdoms prepared by some certain homogenous groups. It was completed by tracing the transfering process to grasp character of social structure and organizing System in the community. For this reason, it's analyzed that Wooden chamber tomb was formed in some certain Time-Space of the Yaksadong Buk-dong archaeological site in the Three Kingdom Period. Constructed group was distinguished by the five classes. So perhaps scale of Wooden chamber and sort, material of burial relics, the top layer and the upper layer were established two status those are active the ruling class led the Wooden chamber tomb's society, the general public hold a large majority from the middle class to the lowest class. And it depends on age(nonage-adult) that differed in relation of arrangement, character. It's changed with function or roles, property, purpose etc. of social community in accordance with these individual position, age. Received signification, value of social status were considered with social worth, faith. Members in a community had been conducted under premeditated, organised system for common target, relatedness. Also it seems to attempt organization's own maintenance, persistiveness in norms that given at common law. To do that, relation for combination between members who consisted systems had to be organic. The relation for combination was considered to form relation for the much larger community in based on blood relationship that most vital part. One social structure had established family(house, household) community in based on individual blood relationship, family communities gather up to develop into relative(blood relative, same race) community gradually. Furthermore, relative(blood relative, same race) communities, that focus on the public good gather up to develop into social community in based on regionalism. It's considered to go through the Development Process of family(house, household) community - relative(blood relative, same race) community - social community.

• Journal of Digital Convergence
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• v.17 no.3
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• pp.139-152
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• 2019

In the fourth industrial revolution and the era of convergence and integration, on the situation that the internal colleges are needed active change included in the improvement of educational quality, I tested it on the purpose of empirical analysis with SPSS v.18 how colleges' efforts on the first periodic Organization Evaluation And Accreditation System(OEAAS) affects on the Perceived Management Performances on the perspective of BSC. As the test result, the Degree of Awareness of Colleges' Efforts on the OEAAS affects on just Colleges' Learning and on Growth. The Degree Propriety of Preparation of the OEAAS affects on Customer Performance, on Internal Process Performance, and, on Finance Performance. And the Degree of Satisfaction of Internal Assessment affects on all of BSC 4 performances. The results of this research could be used on making the management idea of colleges' performance on the OEAAS. In the future, it would be needed advanced researches which are able to make relatedness to the expanse of management performance with the OEAAS.

• Journal of Korea Entertainment Industry Association
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• v.14 no.4
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• pp.251-264
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• 2020

The purpose of this study was to examine the differences and the relationship between basic psychological needs and exercise addiction and to identify effects of To do this, the data were collected using the self-evaluating questionnaires on the exercise addiction, the of basic psychological needs, and the subjective were distributed to 305 participants in general exercise enthusiast for all, and the collected data. This study used the SPSS Statistics 18.0 program to process data. Exploratory factor analysis was performed for validity and Cronbach's α was performed for reliability. In addition, Frequency Analysis was carried out to analyze the demographic characteristics and Pearson's Correlation Analysis, independent t-test, one-way ANOVA, correlation analysis, multiple regression analysis Analysis were performed to verify the correlations and differences among variables. The significance level in this research was set at=.05 and the followings α are derived outcomes. The following is the comprehensive finding of the effect of general exercise enthusiast exercise addiction through basic psychological needs. First, there was no significant difference in basic psychological need and Exercise Addiction of general characteristics, but participation in 6 days was the highest in exercise amount per week. Second, Basic Psychological Need(autonomy, competition, relatedness) showed a high difference in Exercise Addiction(withdrawal symptoms, exercise need, emotional attachment). Third, correlation analysis between all variables showed statistically significant difference. Fourth, Basic Psychological Need(autonomy, competition) was found to have a high effect on Exercise Addiction(withdrawal symptoms, exercise need, emotional attachment).

• Journal of The Korean Association For Science Education
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• v.42 no.2
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• pp.265-275
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• 2022

In this study, focusing on science education in the future society, we explored students' key competencies to be cultivated for the future society, and the role and PCK expertise necessary for science teachers, who are the main agents of competency-based education to nurture these key competencies. A survey conducted among earth science teachers across the country results in 105 valid responses being obtained. The research results are discussed in terms of students' key competencies to be nurtured for the future society, the role and the expertise of science teachers required for competency-based education, and the structural relationship between the teacher role and teacher expertise. We also conducted network analysis to examine the relationship between student competency and teacher expertise, and the structure between the teacher's role and expertise. Main results include that communication and collaboration skills are the most important for students in the future society as core competencies. For science teachers, providing opportunities for collaboration-oriented activities are deemed as the most important. Regarding the structural relationship between the teacher's role and the teacher's expertise, there is a clear relationship with roles such as providing opportunities for collaboration-oriented activities and utilizing various materials and contents in relation to the expertise related to the science teaching practice. Based on the results, ways to promote student's agency based on raising teachers' awareness of the student's competencies, the inter-relatedness of the teacher's role and the teacher's expertise, and the totality of teachers' expertise were suggested.

• Journal of the Korean Applied Science and Technology
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• v.40 no.2
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• pp.233-243
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• 2023

The purpose of this study is to analyze the relationship between self-determination motivation of high school students on exercise satisfaction and exercise continuation intention. To this end, as of March 2022, an online questionnaire was distributed to 440 male students attending high schools in Seoul and Gyeonggi Province, and they were asked to fill out the questionnaire through self-assessment. 346 copies were selected as a valid sample, excluding 94 copies of insincere responses such as responses of the same pattern and incomplete responses. For data processing, frequency analysis, confirmatory factor analysis, and correlation analysis were conducted using SPSS 24.0 and IBM AMOS 18.0 statistical programs, and the following results were derived through structural equation model analysis to identify the structural relationship between factors. First, among self-determination motivation factors, competence and relatedness had a positive (+) effect on exercise satisfaction, but autonomy had a negative (-) effect on exercise satisfaction. Second, there was no relationship between self-determination motivation and exercise continuation intention in high school students. Third, it was found that the relationship between exercise satisfaction and exercise continuation intention had a positive (+) effect.