• Title/Summary/Keyword: Regeneration Mode

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Efficient Callus Induction and Plant Regeneration of Guineagrass (Panicum maximum Jacq.) (난지형 목초 기니아그라스의 효율적인 캘러스 유도 및 식물체 재분화)

  • Seo, Mi-Suk;Takahara, Manabu;Takamizo, Tadashi
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.4
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    • pp.283-290
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    • 2010
  • Guineagrass (Panicum maximum Jacq.) is an important warm-season forage grass as well as biomass crop. It has both sexual and asexual mode of reproduction (apomictic) depending on cultivar. We developed efficient plant regeneration system for an apomictic (cv. Natsukaze) and a non-apomictic (Noh-PL1) guineagrass by optimizing the level of L-proline in the callus induction and that of $AgNO_3$ in plant regeneration medium. Among the L-proline concentrations tested, the best callus induction was achieved by using 2g/L L-proline in both the genotypes. Immature embryos proved to be the best explant source for tissue culture of guineagrass. The highest frequency of shoot regeneration was obtained on MS plant regeneration medium supplemented with 2 mg/L $AgNO_3$. These results provide a foundation for efficient tissue culture and genetic improvement of guineagrass.

The Influence of 5-Fluorouracil Administration Mode on the Expression of Phospholipase C and Ras Oncoprotein Associated with Regeneration of Rat Intestinal Mucosa Following Radiation (방사선 조사후 백서 공장 점막의 재생과정에서 5-fluorouracil 투여가 phospholipsse C 와 ras 암유전자단백의 발현에 미치는 영향)

  • Park Kyung Ran;Lee Chung Sik;Kim Sung Sook;Lee Young Han;Ryu Sung Ho;Suh Pann-Ghill
    • Radiation Oncology Journal
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    • v.12 no.3
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    • pp.271-284
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    • 1994
  • Purpose : Phospholipase C(PLC) isozymes play significant roles in transmembrane signal transduction. PLC-${\gamma}1$ acts as the intracellular effector in signal transduction for cellular proliferation and differentiation. Ras oncoprotein is also involved in cell growth. We determined the biological significance of PLC and ras oncoprotein in regeneration following radiation and the effect of different modes of administration of 5-FU. Materials and Methods : To determine the effect of the administration mode of 5-FU on the regeneration of intestinal mucosa of rats following radiation, we compared the expression of PLC and ras oncoprotein in six groups. Group I had no treatment. Group II received radiation(8 Gy) only. Group III received radiation(8 Gy) and 5-FU(150mg/kg) continuous intravenous (iv) infusion for 12 hours. Group IV received radiation(8 Gy) and 5-FU(750mg/kg) iv bolus injection. Group V received only 5-FU(150mg/kg) continuous iv infusion for 12 hours, Group VI received only 5-FU (150mg/kg) iv bolus injection. Through immunoblotting and immunohistochemistry, we examined the expression of PLC and ras oncoprotein in rat jejunum at 96 hours after radiation or 5-FU administration and at 120 hours after radiation and 5-FU adminstration. We also investigated the histological findings using hematoxylin and eosin stain. Results : In the immunohistochemistry study, PLC-${\gamma}1$ expression was the highest in group III followed by groups II and VI in that order and was weakly positive in groups V and VI. PLC-${\gamma}1$ was hardly detected in the control group. The expression of ras oncoprotein was the same as the PLC-${\gamma}1$ expression for all groups. These results were confirmed by the histological findings regarding the mucosal regeneration. In the immunoblotting analysis, PLC-${\gamma}1$ expression was the highest in group III followed by group IV and II in that order. This difference between the immunoblotting and immunohistochemistry study was due to the high expression of PLC-${\gamma}1$ on the damaged surface epithelium rather than to its expression in the regeneration region as observed in the immunohistochemistry study for group IV. The expression of PLC-${\delta}1$ was positive only in group V and VI, which received both radiation and 5-FU, and the expression of PLC-${\beta}1$ was negligible for all groups. Conclusion : These results suggest that PLC-${\gamma}1$ mediated signal transduetion and ras oncoprotein may have a significant role in mucosal regeneration after radiation, and that continuous iv infusion of 5-FU may induce active regeneration in intestinal mucosa following radiation. In addition, the expression of PLC-${\delta}1$ in combined group of radiation and 5-FU implies that PLC-${\delta}1$ may be involved in signal transduction mediated by concerted action between radiation and 5-FU.

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Regeneration Process of the Pine(Pinus densiflora) Forest in Bulying-Gyegog, Kyungsangbuk-Do, Korea (경북 불영계곡 소나무(Pinus densiflora)임(林)의 재생(再生) 과정(科程)에 관한 연구(硏究))

  • Kim, Seong-Deog;Song, Ho Kyung
    • Journal of Korean Society of Forest Science
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    • v.84 no.2
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    • pp.258-265
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    • 1995
  • Regeneration process of edaphic pine(Pinus densiflora) climax forest in Bulyung-gyegog, Uljin-gun, Kyungsangbuk-do, was studied. The height of dominant trees in the stands are 12 15m. Upper layers are highly dominated by pine trees and are randomly distributed. Pine trees in lower layer grow in a patch type. In the upper layer, the age distribution is likely one model type with mode of 60 and 80 years. In the horizontal distribution of these trees, some of the even-aged cluster constituted of several tree were found. For the first 25 years, the stem diameter increment rates in upper layer are higher than those in the middle layer. It could be concluded that after the forest canopy had been broken out, the seedlings established densely in a patch in the gap with the decreasing density and some of them, distributed in random, would constitute the canopy.

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Genetic Analysis of the Ability of Callus Formation and Plant Regeneration in Seed Culture of Rice (벼의 종자배양에서 캘러스 형성과 식물체 재분화 능력의 유전)

  • 오명진;권용삼;손재근
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.2
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    • pp.77-82
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    • 2000
  • This study was conducted to determine the inheritance of the ability of callus formation and plant regeneration in seed cultures of rice. The culturabilities of three Japonica rices,'Chucheongbyeo', 'Nagdongbyeo', and 'Daeribbyeo 1', were higher than those of Tongil type cultivars, 'Milyang 23' and 'Samgangbyeo 'The frequency for callus growth in F₂ populations of the three crosses, 'Milyang 23/Chucheongbyeo', 'Milyang 23/Daeribbyeo 1', and' Samgangbyeo/Nagdongbyeo', revealed a nearly normal distribution. The broad-sense heritability estimated from the ability of callus formation in the crosses were ranged from 83.8% to 90.1%. The frequency distribution of plant regenerability in F₂ population of 'Milyang 23/Daeribbyeo 1' showed a continuous variation. But the segregation mode of plant regenerability from seed-derived callus in the F₂ progenies of 'Milyang 23/Chucheongbyeo' and 'Samgangbyeo/Nagdongbyeo' appeared to fit the expected 3 : 1 ratio for the high and low regenerability. These results suggest that the high plant regenerability of 'Chucheongbyeo' and 'Nagdongbyeo' was regulated by a single dominant gene.

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Traffic Impacts of Transit-oriented Urban Regeneration (TOD형 도시재생사업의 교통영향 분석)

  • Hwang, Kee Yeon;Cho, Yong Hak
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.4D
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    • pp.469-476
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    • 2008
  • Recently, TOD gains popularity as a traffic solution measure of high density urban regeneration projects. The purpose of this study is to investigate traffic impacts of high density TOD projects, and to identify the issues to be resolved. For a case study, it chooses Gangnamgucheong station in Gangnam area served by two subway lines, and designates 400m radius from the station as a site for high-density development. The MOEs chosen for this study is traffic volume, time, distance, speed, and mode share. The SECOM model is adopted for traffic simulation. The analysis results show that high-density TOD is an effective tool for traffic improvement even with only one station area being implemented. It is found that the traffic volume increases near the station in nature where high-density development occurs, but it declines overall in the rest of Gangam area. The total travel time and distance of passenger vehicles decline, meaning that the traffic condition becomes better than before. With regulation on parking supply, the improvement becomes more vivid. In terms of the changes of traffic speed, both alternatives show 4.1% increase in speed, but the difference between alternatives is not quite noticeable because of the induced vehicle demand driven to the streets with improved traffic condition. The mode share changes occur for the benefit of subway ridership, because the study station is equipped with two subway line services. When mixed with parking supply restriction, the impact becomes clearer.

AC Regeneratable Battery Charging and Discharging Test System (AC 회생이 가능한 배터리 충·방전 테스트 시스템)

  • Kim, Jun-Gu;Youn, Sun-Jae;Kim, Jae-Hyung;Won, Chung-Yuen;Na, Jong-Kuk
    • The Transactions of the Korean Institute of Power Electronics
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    • v.17 no.2
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    • pp.99-106
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    • 2012
  • In this paper, 15[kW] AC regenerative system for battery charging and discharging test is proposed. The regenerative system is able to regenerate surplus energy to the grid in discharging mode, and the inverter of the system can be operated as a converter to compensate scarce energy in charging mode. In case of the conventional DC charging and discharging system, the regenerative energy is consumed by a resistor. However, as the proposed system regenerates the surplus energy to the grid through using DC-AC inverter, the energy saving effect can be achieved. In this paper, 15[kW] battery charging and discharging system is developed, and the validity of the system is verified through simulation and experimental results.

Particle Emission Characteristics of Heavy-duty Diesel Engine using Aftertreatment Systems (후처리장치 부착에 따른 대형디젤엔진의 입자 배출특성)

  • Kwon, Sangil;Park, Yonghee
    • Journal of ILASS-Korea
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    • v.17 no.3
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    • pp.146-151
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    • 2012
  • This study was primarily focused on the experimental comparison of the particle emission characteristics for heavy duty engine. PM and particle number from various heavy duty engines and DPF type were analyzed with a golden particle measurement system recommended by the Particle Measurement Program. And the repeatability and reproducibility between test mode was analyzed. This study was conducted for the experimental comparison on particulate emission characteristics between the European and World-Harmonized test cycles for a heavy-duty diesel engine. To verify the particulate mass and particle number concentrations from various operating modes, ETC/ESC and WHTC/WHSC, both of which will be enacted in Euro VI emission legislation, were evaluated. Real-time particle formation of the transient cycles ETC and WHTC were strongly correlated with engine operating conditions and after-treatment device temperature. A higher particle number concentration during the ESC mode was ascribed to passive DPF regeneration and the thermal release of low volatile particles at high exhaust temperature conditions.

Low-Cost, Low-Power, High-Capacity 3R OEO-Type Reach Extender for a Long-Reach TDMA-PON

  • Kim, Kwang-Ok;Lee, Jie-Hyun;Lee, Sang-Soo;Lee, Jong-Hyun;Jang, Youn-Seon
    • ETRI Journal
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    • v.34 no.3
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    • pp.352-360
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    • 2012
  • This paper proposes a low-cost, low-power, and high-capacity optical-electrical-optical-type reach extender that can provide 3R frame regeneration and remote management to increase the reach and split ratio with no change to a legacy time division multiple access passive optical network. To provide remote management, the extender gathers information regarding optical transceivers and link status per port and then transmits to a service provider using a simple network management protocol agent. The extender can also apply to an Ethernet passive optical network (E-PON) or a gigabit-capable PON (G-PON) by remote control. In a G-PON, in particular, it can provide burst mode signal retiming and burst-to-continuous mode conversion at the upstream path through a G-PON transmission convergence frame adaptor. Our proposed reach extender is based on the quad-port architecture for cost-effective design and can accommodate both the physical reach of 60 km and the 512 split ratios in a G-PON and the physical reach of 80 km and the 256 split ratios in an E-PON.

High Efficiency Power Conversion Device for Photovoltaic Power Generation (태양광 발전을 위한 고효율 전력변환장치)

  • Kim, Young-Cheal;Suh, Ki-Young;Lee, Hyun-Woo
    • Proceedings of the KIEE Conference
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    • 1996.07a
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    • pp.450-452
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    • 1996
  • In this paper, the authors propose a DC-DC boost converter of high efficiency by partial resonant switching mode, the switching devices in a proposed circuit are operated with soft switching and the control technique of those is simplified for switch to drive in constant duty cycle. The circuit has a merit which is taken to increase of efficiency, as it makes to a regeneration at input source of accumulated energy in snubber condenser without loss of snubber inconventional circuit. The proposed converter is deemed the most suitable for high power applications where the power switching devices are used.

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Development of Flywheel Energy Storage System Using Superconducting Magnetic Bearing (초전도 플라이휠 에너지 저장시스템의 개발)

  • 정환명;연제욱;최재호;고창섭
    • Proceedings of the KIPE Conference
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    • 1999.07a
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    • pp.427-430
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    • 1999
  • This paper presents a S-FES(Superconducting magnetic bearing Flywheel Energy Storage System) for the purpose of replacing battery used to store the energy. Especially, the design elements of FES, such as the bearing, wheel material, and power converter, etc., is described. The design and manufacturing techniques of the controllable power converter are proposed to generate the sinusoidal output current in the high speed operation and to get the constant DC voltage in the regeneration mode. The cylindrical permanent magnet synchronous motor with halbach array of Nd-Fe-B permanent magnet which is the high coercivity material is used as the driver of FES. The proposed S-FES system shows the stable rotation characteristics at high speed range about 10,000 rpm. To verify the validity of proposed system, the comparative study with the conventional ball bearing system is proceeded and it is well confirmed with the result of the lower friction loss of S-FES system.

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