Genetic Analysis of the Ability of Callus Formation and Plant Regeneration in Seed Culture of Rice

벼의 종자배양에서 캘러스 형성과 식물체 재분화 능력의 유전

  • 오명진 (경북대학교 농과대학 농학과) ;
  • 권용삼 (경북대학교 농과대학 농학과) ;
  • 손재근 (경북대학교 농과대학 농학과)
  • Published : 2000.03.01

Abstract

This study was conducted to determine the inheritance of the ability of callus formation and plant regeneration in seed cultures of rice. The culturabilities of three Japonica rices,'Chucheongbyeo', 'Nagdongbyeo', and 'Daeribbyeo 1', were higher than those of Tongil type cultivars, 'Milyang 23' and 'Samgangbyeo 'The frequency for callus growth in F₂ populations of the three crosses, 'Milyang 23/Chucheongbyeo', 'Milyang 23/Daeribbyeo 1', and' Samgangbyeo/Nagdongbyeo', revealed a nearly normal distribution. The broad-sense heritability estimated from the ability of callus formation in the crosses were ranged from 83.8% to 90.1%. The frequency distribution of plant regenerability in F₂ population of 'Milyang 23/Daeribbyeo 1' showed a continuous variation. But the segregation mode of plant regenerability from seed-derived callus in the F₂ progenies of 'Milyang 23/Chucheongbyeo' and 'Samgangbyeo/Nagdongbyeo' appeared to fit the expected 3 : 1 ratio for the high and low regenerability. These results suggest that the high plant regenerability of 'Chucheongbyeo' and 'Nagdongbyeo' was regulated by a single dominant gene.

벼 종자배양에서 식물체 재분화 능력이 서로 다른 품종간에 교배된 잡종집단을 대상으로 캘러스 형성과 식물체 재분화 능력에 대한 유전양식을 구명한 결과는 다음과 같다. '밀양 23호/추청벼', '삼강벼/낙동벼' 및 '밀양 23호/대립벼 1호'조합 F₂집단의 캘러스 생장량에 대한 빈도분포는 3조합 모두 정규분포에 가까운 연속적인 분포양상을 나타내었으며, 각 조합에서 추정된 광의의 유전력은 조합에 따라 83.8%에서 90.1%로 높게 나타났다. 식물체 분화율의 경우 조합에 따라 유전양상이 다르게 나타났는데, '대립벼 1호/밀양 23호' 조합은 F₂집단의 평균 식물체 분화율이 43.6%로 양친의 평균치 보다 높게 나타나면서 빈도분포 면에서 캘러스 생체중과 동일한 양상을 보여 우성으로 작용하는 다수의 유전자에 의해 지배되는 것으로 나타났으며, 식물체 재분화 능력에 대한 광의의 유전력은 83.4%로 높게 추정되었다. 그러나 '밀양 23호 /추청벼' 조합과 '삼강벼/낙동벼' 조합의 경우는 식물체 재분화 능력이 높은 것과 낮은 것이 3 : 1의 이론적 분리비에 적합한 것으로 나타나 '추청벼'와 낙동벼'의 식물체 재분화에는 하나의 우성유전자가 관여하는 것으로 조사되었다.

Keywords

References

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