• Journal of Plant Biotechnology
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• 제48권2호
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• pp.86-92
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• 2021

The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%).

• 한국해양학회지:바다
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• 제7권2호
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• pp.60-67
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• 2002

황해 대륙붕 해역의 전 수주(whole water column)의 질소 섭취율 중 무광대(aphotic zone)내 식물플랑크톤에 의한 질소 섭취율의 기여도를 파악하기 위하여 상부의 유광대(euphotic zone)와 하부의 무광대에서 식물플랑크톤에 의한 질소 섭취율의 크기를 측정하였다. 1997년 5월과 11월에 유광대와 무광대에서 일정량의 해수를 채취하여 선상에서 식물플랑크톤의 질산염과 암모늄 섭취율 측정을 $^{15}N$으로 표지하여 추적하였다. 전 유광대내의 질산염 및 암모늄 섭취율은 각각 1.8${\sim}$15.3 mg N $m^{-2}$ $d^{-1}$과 5.0${\sim}$132.2 mg N $m^{-2}$ $d^{-1}$의 범위이며, 대체로 암모늄 섭취율이 질산염 섭취율보다 우세하였다(1.9${\sim}$19.4배). 전 수주내의 질산염 및 암모늄 섭취율은 각각 2.9${\sim}$22.0 mg N$m^{-2}$ $d^{-1}$과 15.7${\sim}$175.5 mg N$m^{-2}$ $d^{-1}$의 범위를 나타냈다. 전 수주내의 총 질소 섭취율 중 무광대의 식물플랑크톤에 의한 질소 섭취율이 차지하는 비율은 질산염의 경우에 13.0${\sim}$86.2%, 암모늄의 경우에는 13.8${\sim}$67.8%로서, 무광대에서 식물플랑크톤에 의한 질소 섭취가 상당함이 발견되었다. 본 연구의 결과는 무광대내에서 상당한 양으로 활발하게 질소를 섭취하는 식물플랑크톤이 물리적 작용들(예를 들면, 와류 혼합, 수온약층의 수직이동 등)에 의해 유광대로 다시 편입(entrainment)될 경우 유광대의 물질 순환에 크게 기여할 가능성이 있음을 시사한다. 그리고, 본 연구 결과는 황해에서 신생산(new production)의 크기가 질산염 섭취로만 추정될 경우 과소평가될 가능성이 있으며, 암모늄 섭취에 기초한 재생산(regenerated production)의 일부가 신생산에 포함되어야 함을 제시한다.

• Journal of the korean society of oceanography
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• 제31권4호
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• pp.196-206
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• 1996

Vertical profiles of the chlorophyll ${\alpha}$, phytoplankton abundance, nutrients and sigma-t were compared with the vertical distribution of phytoplankton species in conjunction with $^{14}$C primary production in the Southern Waters of the East Sea, Korea. In the upper mixed layer the water column was only weakly stratified and ambient nitrogenic nutrient concentrations were markedly depleted. Dissolved silicate seemed to be another limiting nutrient in the surface layer. The occupation of different water depths by several dominant diatom species was well explained by the degree of silicification of each cell and the silicate concentration of ambient seawater. Subsurface chlorophyll maxima were continuously observed in the lower parts of the euphotic layer and the depth coincided with nutricline, supporting our view that chlorophyll maximum was sustained partially by enhancement of in situ growth of phytoplankton and partially by increase of cellular chlorophyll content. The persistence of chlorophyll maximum layer was attributed to the physiological adaptation of the phytoplankters to low light intensities and to the utilization of regenerated nutrients. Integrated water column production of organic matter by photosynthesis appeared to be better related to phytoplankton cell division than to the cell growth in terms of biosynthesis of pigments and other intracellular components.

• KSBB Journal
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• 제7권3호
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• pp.179-185
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• 1992

NAD재생형 bioreactor를 구축함에 있어 음이온 하전막을 이용하고자 하였다. 사용한 막은 용액상의 NAD의 통과를 저지하여 80.9%를 bioreactor내에 존속시켰는데 NAD의 bioreactor내에의 존속율은 Tris-maleate완충용액을 사용하고 albumin을 첨가하므로써 증가되었다. 음이온하전막을 장착한 bioreactor내에서 주반응 효소로서 sorbitol dehydrogenase와 재생반응효소로서 alcohol dehydrogenase를 사용하여 NADH로의 재생과 함께 fructose로부터 sorbitol의 생산을 행하였다. 반응형식은 반복회분조작을 행하였는데 매 회분마다 70%의 전환율이 될 때까지 반응기킨 후 반응액의 90%를 회수한 후 fructose와 ethanol을 feed하여 다음의 회분반응을 행하였다. Sobitol의 생산량은 198시간의 반응에서 47g/L이었다. 한편 사용한 막의 NAD존속율은 반응종료후에도 거의 감소되지 않았다.

• 한국수산과학회지
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• 제38권6호
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• pp.393-398
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• 2005

Codium fragile is commercially farmed in Korea by natural blooming zygote attachment. Experiments found optimum conditions for artificial seed production and nursery culture of C. fragile by asexual reproduction. Isolated utricles and medullary filaments were regenerated to erect thalli using both indoor and outdoor culture experiments. Under the indoor culture conditions, irradiance was an important factor to control the development of erect thalli. Formation of erect thallus from the isolated medullary filaments in the indoor culture was induced after 30 days under $20^{\circ}C$ and $60{\mu}mol/m^2/sec$. The detachment of isolated utricles and medullary filaments from the substrates of seed strings was reduced by exposure to the air during 2 hrs before the indoor culture of seed strings. The maximum growth and development of erect thalli in the nursery culture was induced at a water depth of 0.5 m. Depending on the substrates of the seed strings the growth of erect thalli was not significantly different (p>0.05).

• 한국자원식물학회지
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• 제34권2호
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• pp.156-165
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• 2021

바이오에너지작물의 중요한 소재를 제공하는 화본과 식물인 홍띠(Imperata cylindrica 'Rubra')의 기내재생 식물체의 유전적 안정성을 조사하고자 생장점 부위를 기내배양하여 재분화시킨 녹색 재생식물체의 변이성을 ISSR 마커로 조사하였다. MS(Murashige and Skoog, 1962) 배지에 식물체 기부의 생장점 부위를 적출하여 캘러스를 유도하고(0.1 mg/L 2,4-D와 2 mg/L BA), 캘러스 증식(0.1 mg/L 2,4-D와 0.05 mg/L BA), 신초 재분화(0.01 mg/L NAA와 2 mg/L BA) 후 MS 배지에서 발근시켜 재분화 식물체를 유도하고 100% 활착시켰다. 대조구인 모식물체 홍띠 8개체, 1년간 노지 포장에서 재배중인 재분화 녹색 식물체 10개체와 실험실 내 화분에서 재배중인 재분화 녹색 식물체 10개체, 총 28개체에 대하여 ISSR 분석한 결과 유전적 다형성은 재분화 식물체가 실내포트 재배식물체 4.1% 및 노지 재배식물체 3.1%로 0%인 대조구보다 높게 나타났다. 또한, 총 28개체들 간의 유전적 유사도를 평가한 결과, 유전적 유사도 지수는 0.919~1.0 사이에 분포하며, 평균 0.972로 유전적 충실도가 높게 나타났다. 군집분석 결과 노지에서 재배한 재분화 식물체와 모식물체(대조군)가 독립적으로 유집되었다.

• Plant Biotechnology Reports
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• 제5권3호
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• pp.265-271
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• 2011

The cultivated potato as well as its tuber-bearing relatives are considered model plants for cell and tissue culture, and therefore for exploiting the genetic variation induced by in vitro culture. The association between molecular stability and tissue culture in different genetic backgrounds and ploidy levels has already been explored. However, it still remains to be ascertained whether somaclonal variation differs between callus-derived chromosome-doubled and undoubled regenerants. Our research aimed at investigating, through amplified fragment length polymorphism (AFLP) markers, the genetic changes in marker-banding patterns of diploid and tetraploid regenerants obtained from one clone each of Solanum bulbocastanum Dunal and S. cardiophyllum Lindl (both 2n = 2x = 24) and tetraploids from cultivated S. tuberosum L. (2n = 4x = 48). Pairwise comparisons between the banding patterns of regenerants and parents allowed detecting considerable changes associated to in vitro culture both at diploid and tetraploid level. The percentages of polymorphic bands between diploid and tetraploid regenerants were, respectively, 57 and 69% in S. bulbocastanum and 58 and 63% in S. cardiophyllum. On average, the frequencies of lost parental fragments in regenerants were significantly higher than novel bands both in S. bulbocastanum (48 vs. 22%) and S. tuberosum (36 vs. 18%) regenerants. By contrast, in S. cardiophyllum, a similar incidence of the two events was detected (32 vs. 29%). Our results revealed that structural changes after tissue culture process strongly affected the genome of the species studied, but diploid and tetraploids regenerated plants responded equally.

• 한국작물학회지
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• 제59권4호
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• pp.498-504
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• 2014

Potato virus Y (PVY) and potato leafroll virus (PLRV) are among the most damaging potato viruses and prevalent in most potato growing areas. In this study, cryopreservation was used to eradicate PVY and PLRV using two cryogenic methods. Potato shoot tips proliferated in vitro were cryopreserved through droplet-vitrification and encapsulation-vitrification using plant vitrification solution 2 (PVS2; 30% glycerol + 15% dimethyl sulfoxide + 15.0% ethylene glycol + 13.7% sucrose) and modified PVS2. Both cryogenic procedures produced similar rates of survival and regrowth, which were lower than those from shoot tip culture alone. The health status of plantlets regenerated from shoot tip culture alone and cryopreservation was checked by reverse transcription-polymerase chain reaction. The frequency of virus-free plants regenerated directly from highly proliferating shoot tips reached 42.3% and 48.6% for PVY and PLRV, respectively. In comparison, the frequency of PVY and PLRV eradication after cryopreservation was 91.3~99.7% following shoot-tip culture. The highest cryopreserved shoot tip regeneration rate was observed when shoot tips were 1.0~1.5 mm in length, but virus eradication rates were very similar (96.4~99.7%), regardless of shoot tip size. This efficient cryotherapy protocol developed to eliminate viruses can also be used to prepare potato material for safe long-term preservation and the production of virus-free plants.

• 한국작물학회지
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• 제51권spc1호
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• pp.237-241
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• 2006

국내육성 유채품종 간 반수체식물 생산성을 비교하였다. 화아로부터 분리된 소포자를 13%의 sucrose, $0.05mg/{\ell}$ BA 와 $0.5mg/{\ell}$ 의 NAA가 첨가된 NLN 배지에서 배양하였다. 반수체 배의 생산에는 유전형이 중요한 요인이였으며, 탐라유채가 가장 높은 반수체 배 생산능을 보여 화아당 176개의 반수체 배가 발생하였으나, 한라유채와 영산유채는 배 생산은 물론 세포분열도 관찰되지 않았다. 발생한 반수체 배를 NLN배지에서 현탁배양하여 Multilobe abnormal embryos를 형성시켰으며, 계속하여 생장조절제가 첨가되지 않은 MS고체 배지에 치상 배양하여 반수체식물체를 유도 하였다. 재생된 반수체 식물체는 성공적으로 순화되었다.

• 한국환경과학회지
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• 제12권8호
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• pp.841-846
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• 2003

The ecosystem model was applied to estimate the regional distribution of the net production(or consumption) of phytoplankton and the net uptake(or regeneration) rate of nutrients in Masan Bay for scenario analysis to find a proper management plan. At the surface level, net production of phytoplankton is 200 mgC/㎡/day at the entrance of the bay, and 400∼1000 mgC/㎡/day at the center of the bay. The inner area of the bay showed more than 2000 mgC/㎡/day. All areas of the bottom level have a net consumption, with the center of the bottom level showing more than 600 mgC/㎡/day. For dissolved inorganic nitrogen, the results showed a net uptake rate of 100∼900 mg/㎡/day at the surface level. It showed that the net regeneration is above 50 mg/㎡/day at the bottom level. For dissolved inorganic phosphorus, the net uptake rate showed 10.0∼80.0 mg/㎡/day at the surface level, and the regeneration rate showed 0∼20.5 mg/㎡/day at the bottom level. Therefore, in order to control the water quality in Masan Bay, it is important to consider the re-supplement of nutrients regenerated in the water column.