• 제목/요약/키워드: Reductase

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시금치 nitrate reductase cDNA 클로닝 및 염기서열 분석 (Cloning and Sequence Analysis of Spinach (Spinacia oleracea L. cv Ace) Nitrate Reductase cDNA)

  • 박누리;정종배;박상규
    • Applied Biological Chemistry
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    • 제45권3호
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    • pp.129-133
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    • 2002
  • 키토산 분해물을 시금치와 상추에 살포하였을 때, nitrate 함량이 감소되었으며, 이러한 감소는 nitrate reductase 활성의 증가에 기인한 것으로 나타났다. 이에 따라 채소 중 질산염을 가장 많이 축적하는 채소 중 하나인 시금치의 nitrate reductase를 식물체내에 과량 발현시켜 질산염 축적을 줄이기 위한 연구를 수행하였다. 첫 단계로 시금치 mRNA로부터 RT-PCR을 이용하여 cDNA를 분리, 증폭하고 벡터에 클로닝하여 염기서열을 결정하였다. 시금치 nitrate reductase cDNA의 염기서열은 다른 식물체에서 분리된 nitrate reductase 유전자들과 상당히 높은 상동성($71{\sim}82%$)을 보였고, 이미 발표된 시금치 nitrate reductase cDNA의 염기서열과 비교하였을 때 단지 두 염기만이 달랐다.

THE EFFECT OF DIETARY FATS ON THE HEPATIC AND INTESTINAL 3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE ACTIVITIES IN CHICKS

  • Youn, B.S.;Tananka, K.;Ohtani, S.;Santoso, U.
    • Asian-Australasian Journal of Animal Sciences
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    • 제6권2호
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    • pp.281-290
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    • 1993
  • This experiment was designed to evaluate the effect of degree of unsaturation (Experiment 1) and the chain length of constituent fatty acids of dietary fats (Experiment 2) on-3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activities in the liver and small intestine of chicks. Chicks were fed experimental diets for 10 days and then killed for the determination of the HMG-CoA reductase activities in the intestinal epithelial cell and hepatic microsomes. The hepatic HMG-CoA reductase activity showed the highest value in chicks fed the tallow-containing diet. Chicks fed diets containing safflower or coconut oil resulted in a significantly lower intestinal HMG-CoA reductase activity in comparison with those fed the olive oil-containing diet. The hepatic HMG-CoA reductase activity was significantly higher when fat-free and trilaurin were fed than when any other triglycerides were fed. This activity showed the lowest value in the chicks fed the diet containing tristearin. The HMG-CoA reductase activities in the jejunum and ileum were significantly or tended to be higher when trilaurin was fed than when any other triglycerides were fed. Except when trilaurin was fed, the presence of saturated fat in the diet did not have a significant effect on the intestinal HMG-CoA reductase activity, unlike the effect shown when a highly unsaturated fat was added to the diet. There was no significant correlation between the HMG-CoA reductase activities of the liver and intestinal, and the HMG-CoA reductase activity and cholesterol content of the intestinal epithelial cells.

Purification of the NADH Reductase Component of the Steroid $9{\alpha}$-hydroxylase from Mycobacterium fortuitum

  • Kang, Hee-Kyoung;Lee, Sang-Sup
    • Archives of Pharmacal Research
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    • 제20권6호
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    • pp.590-596
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    • 1997
  • The NADH reductase component of the steroid 9.alpha.-hydroxylase from Mycobacterium fortuitum was purified to homogeneity. Recovery of the enzyme from the 50-60% ammonium sulfate saturated fraction was 49%, with a purification factor of 100-fold. The NADH reductase has a relative molecular of 60 KDa as determined by SDS-PAGE. The absorption maxima at 410 and 450 nm indicate the presence of iron-sulfur group and flavin. These prosthetic groups seemed to function as redox groups that transfer electrons from NADH to the following protein. The $K_M$ value for NADH as substrate was $68{\mu}M$. The $NH_2$-terminal amino acid sequence of the reductase was determined as Met-Asp-Ala-Ile-Thr-Asn-Val-Pro-Leu-Pro-Ala-Asn-Glu-Pro-Val-His-Asp-Tyr-Ala-Thr. This sequence does not show a homology with the $NH_2$ -terminal sequences reported for the reductase component of other monooxygenases, suggesting that the NADH reductase component of the steroid 9.alpha.-hydroxylase system is novel.

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Modeled structure of trypanothione reductase of Leishmania infantum

  • Singh, Bishal K.;Sarkar, Nandini;Jagannadham, M.V.;Dubey, Vikash K.
    • BMB Reports
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    • 제41권6호
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    • pp.444-447
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    • 2008
  • Trypanothione reductase is an important target enzyme for structure-based drug design against Leishmania. We used homology modeling to construct a three-dimensional structure of the trypanothione reductase (TR) of Leishmania infantum. The structure shows acceptable Ramachandran statistics and a remarkably different active site from glutathione reductase(GR). Thus, a specific inhibitor against TR can be designed without interfering with host (human) GR activity.

Association of a Common Reductase with Multiple Aromatic Terminal Dioxygenases in Sphingomonas yanoikuyae Strain B1

  • Mihyun Bae;Kim, Eungbin
    • Journal of Microbiology
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    • 제38권1호
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    • pp.40-43
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    • 2000
  • The aromatic dioxygenase system in Sphingomonas yanoikuyae strain Bl consists of three components, an oxygenase, a ferredoxin, and a reductase. The insertional knockout of the bphA4 gene encoding a reductase and subsequent complementation experiments showed that the reductase encoded by bphA4 in S. yanoikuyae strain Bl is associated with multiple dioxygenase components including that of toluate dioxygenase (XyIXY).

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Isoflavonoids from the Rhizomes of Belamcanda chinensis and Their Effects on Aldose Reductase and Sorbitol Accumulation in Streptozotocin Induced Diabetic Rat Tissues

  • Jung, Sang-Hoon;Lee, Yeon-Sil;Lee, Sanghyun;Lim, Soon-Sung;Kim, Yeong-Shik;Shin, Kuk-Hyun
    • Archives of Pharmacal Research
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    • 제25권3호
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    • pp.306-312
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    • 2002
  • Aldose reductase, the key enzyme of the polyol pathway, is known to play important roles in the diabetic complication. The inhibitors of aldose reductase, therefore, would be potential agents for the prevention of diabetic complications. To evaluate active principles for the inhibition of aldose reductase from the rhizomes of Belamcanda chinensis, twelve phenolic compounds were isolated and tested for their effects on rat lens aldose reductase. As a result, isoflavones such as tectorigenin, irigenin and their glucosides were found to show a strong aldose reductase inhibition. Tectoridin and tectorigenin, exhibited the highest aldose reductase inhibitory potency, their $IC_{50}$ values, being $1.08{\times}10^{-6}{\;}M{\;}and{\;}1.12{\times}10^{-6}{\;}M$, respectively, for DL-glyceraldehyde as a substrate. Both compounds, when administered orally at 100 mg/kg for 10 consecutive days to streptozotocin-induced diabetic rats, caused a significant inhibition of sorbitol accumulation in the tissues such as lens, sciatic nerves and red blood cells. Tectorigenin showed a stronger inhibitory activity than tectoridin. From these results, it is suggested that tectorigenin is attributed to be a promising compound for the prevention and/or treatment of diabetic complications.

Bacillus cereus D-3로부터 항고지혈증 HMG-CoA Reductase 저해제의 생산 (Production of an Antihyperlipemial HMG-CoA Reductase Inhibitor from Bacillus cereus D-3)

  • 이대형;이재원;정재홍;이종수
    • 한국미생물·생명공학회지
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    • 제34권1호
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    • pp.52-57
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    • 2006
  • 새로운 고지혈증 예방 제품 개발을 위한 기초 자료를 얻고자 우선 전통 된장으로부터 HMG-CoA reductase 저해 물질을 강력하게 세포외로 생산하는 D-3 세균을 최종 분리 하였다. D-3 균의 미생물학적 특성을 조사한 결과 그람양성의 간균으로 운동성이 있었고 통성 혐기성이였다. 이들 특성과 16S rRNA 염기서열의 분석 결과 등을 종합하여 Bergey's manual로 동정한 결과 Bacillus cereus D-3로 동정되었다. HMG-CoA reductase 저해물질 생산 최적 조건을 조사한 결과 Bacillus cereus D-3를 glucose 2%, corn steep liquor $0.6%,\;K_{2}HPO_4\;0.04%,\;KH_{2}PO_4\;0.05%$를 함유한 Glucose-CSL 배지에 접종하여 $30^{\circ}C$에서 36시간 배양하여 얻은 상등액의 HMC-CoA reductase 저해활성이 39.4% 저해물질을 가장 많이 생성 하였다.

울금 추출물의 5-Alpha Reductase II 활성 저해 효과 (Inhibitory Effect of Curcuma longa L. Extracts on 5-Alpha Reductase II Activity)

  • 김종연;이정윤;윤호근;김용재;전우진;황권택;차민석;이유현
    • 한국식품영양과학회지
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    • 제43권2호
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    • pp.318-322
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    • 2014
  • 본 연구에서는 울금(Curcuma longa L.)의 다양한 추출물을 대상으로 5-alpha reductase의 활성 저해 정도를 측정하였다. 5-Alpha reductase는 T에서 활성형 남성호르몬인 DHT로 전환을 담당하며, 전립선비대에서는 혈액에서 DHT 농도가 높다. Rat의 전립선조직 균질액과 5-alpha reductase를 과발현시킨 LNCaP 세포 균질액을 대상으로 측정한 결과, $100{\mu}g/mL$의 농도에서 울금의 열수추출물(CL-HW)과 20% 에탄올추출물(CL-E20)이 5-alpha reductase 활성을 효과적으로 저해하였다. 특히 CL-HW는 $100{\mu}g/mL$의 농도에서 80% 이상의 유의적 억제효과를 보였으며, CL-E20은 $50{\mu}g/mL$의 농도부터 80% 이상의 감소효과를 보였다. 이 같은 결과에서 CL-HW 및 CL-E20은 5-alpha reductase의 억제활성이 있으며, 이는 전립선비대 치료소재로 개발될 수 있는 가능성이 있음을 제안한다.

Effects of Electron Donors on Nitrate Removal by Nitrate and Nitrite Reductases

  • Yoo, Young-Je;Sung, Dong-Won;Song, Seung-Hoon;Kim, Ji-Hyeon
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제7권2호
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    • pp.112-116
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    • 2002
  • Effects of artificial electron donors to deliver reducing power on enzymic denitrification were investigated using nitrate reductase and nitrite reductase obtained from Ochrobactrum antroyi. The activity of nitrite reductase in the soluble portion was almost the same as that in the precipitated portion of the cell extract. Nitrate removal efficiency was higher with benzyl viologen than with methyl viologen or NADH as an artificial electron donor. The turn-over numbers of nitrate and nitrite reductase were 14.1 and 1.9 umol of nitrogen reduced/min$.$mg cell extracts, respectively when benzyl viologen was used as an electron donor.

Inhibition of 5α-reductase of de novo Generation of Short Anti-oxidant Peptides

  • Lee, Sung-Gyu;Kang, Hyun
    • 대한의생명과학회지
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    • 제24권3호
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    • pp.263-269
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    • 2018
  • This study aims to investigate the biological activities related to hair loss of short anti-oxidant peptides (DK peptides) $5{\alpha}$-reductase inhibition and anti-oxidation. The series of DK peptides were generated amphipathic helical properties using leucines, lysines and tryptophan residues. Cell viability and free radical scavenging activities were performed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The DK peptides were investigated for inhibitory activity against $5{\alpha}$-reductase. Antioxidant activities were determined by means of, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays. All peptides could inhibit $5{\alpha}$-reductase in lipopolysaccharide-stimulated macrophage. In conclusion, DK peptides was suggested as the most attractive ingredients for improving hair loss, because of the high inhibitory against $5{\alpha}$-reductase inhibition.