Association of a Common Reductase with Multiple Aromatic Terminal Dioxygenases in Sphingomonas yanoikuyae Strain B1

  • Mihyun Bae (Department of Biology and Institute of Life Science, Yonsei University) ;
  • Kim, Eungbin (Department of Biology and Institute of Life Science, Yonsei University)
  • Published : 2000.03.01

Abstract

The aromatic dioxygenase system in Sphingomonas yanoikuyae strain Bl consists of three components, an oxygenase, a ferredoxin, and a reductase. The insertional knockout of the bphA4 gene encoding a reductase and subsequent complementation experiments showed that the reductase encoded by bphA4 in S. yanoikuyae strain Bl is associated with multiple dioxygenase components including that of toluate dioxygenase (XyIXY).

Keywords

References

  1. Appl. Environ. Microbiol. v.64 Plasposons: modular selfcloning minitransposon derivatives for rapid genetic analysis of gram-negative bacterial genomes Dennis, J.J.;G.J. Zylstra
  2. Science 222 Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesis of indigo Ensley, B.D.;B.J. Ratzkin;T.D. Osslund;M.J. Simon;L.P. Wackett;D.T. Gibson
  3. Appl. Environ. Microbiol. v.59 Enhanced biodegradation of polychlorinated biphenyls after site-directed mutagenesis of a biphenyl diosygenase gene Erickson, B.D.;F.J. Mondello
  4. Proc. Natl. Acad. Sci. USA 76 Replication of and origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans Figurski, D.H.;D.R. Helinski
  5. J. Bacteriol. v.177 Purification and characterization of the oxygenase component of biphenyl 2,3-diox-ygenase from Pseudomonas sp. Strain LB400 Haddock, J.D.;D.T. Gibson
  6. J. Bacteriol. v.175 Oxidation of biphenyl by a multicomponent enzyme system from Pseudomonas sp. Strain LB400 Haddock, J.D.;L.M. Nadim;D.T. Gibson
  7. J. Bacteriol. v.173 Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWWO plasmid xylXYZ genes, encoding benzoate dioxygenases Harayama, S.;M. Rekik;A. Bairoch;E.L. Neidle;L.N. Ornston
  8. Gene v.138 Construction of hybrid bipheny1 (bph) and toluene (tod) genes for functional anlysis of aromatic ring dioxygenases Hirose, J.;A. Suyama;S. Hayashida;K. Furukawa
  9. Gene v.70 Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria Keen, N. T.;S. Tamaki;D. Kobayashi;D. Trollinger
  10. Internat. J. Syst. Bacteriol. v.46 Reclassification of a polycyclic aromatic hydrocarbon-metabolizing bacterial strain Beijerinckia sp. strain B1, as Sphingomonas Yanoikuyae by fatty acid anlaysis, Protein pattern analysis, and 16S rDNA sequencing Khan, A.A.;R.-F. Wang;W.-W. Cao;W. Franklin;C.E. Cerniglia
  11. Ph. D. thesis. Ruters, The State University of New Jersey Kim, E.
  12. J. Indust. Microbiol. Biotechnol. v.23 Functional analysis of genes Involved in biphenyl, naphthalene, phenanthrene, and m-xylene degradation by Sphingomonas yanoikuyae B1 Kim, E.;G.J. Zylstra
  13. FEMS Microbiol. Lett. v.153 Evidence for the role of 2-hydroxy-chromene-2-carboxylate isomerase in the degradation of antracene by Sphingomonas yanoikuyae B1 Kim, E.;G.J. Zylsra;J.P. Freeman;T.M. Heinze;J. Deck;C.E. Cerniglia
  14. J. Bacteriol. v.177 Molecular and biochemical characterization of two meta-cleavae dioxygenases involved in biphenyl and m-xylene degradation by Beijerinckia sp. Strain B1 Kim, E.;G.J. Zylstra
  15. Appl. Environ. Microbiol. v.43 Rapid screen for bacteria degrading water-insoluble solid hydrocarbons on agar plates Kiyohara, H.;K. Nagao;K. Yano
  16. Gene v.166 Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying defferent antibiotic-resistance cassettes Kovach, M.E.;P.H. Elzer;D.S. Hill;G.T. Robertson;M.A. Farris;R. M. RoopⅡ, and K.m. Perterson
  17. J. Bac- teriol. v.173 Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary relationships among multicomponent oxygenases Neidle, E.L.;C. Hartnett;L.N. Ornston;A. Bairoch;M. Rekik;S. Harayama
  18. J. Bacteriol. v.176 Pseudomonas aeruginosa 142 uses a three-component ortho-halobenzoate 1,2-dioxyenase for metabolism of 2,4-dichloro-and 2-chlorobenzoate Romanov, V.;R.P. Hausinger
  19. J. Gen. Microbiol. v.43 The aerobic pseudomonads:a taxonomic study Stanier, R.Y.;N.J. Palleroni;M. Duodoroff
  20. Biochem. Biophys. Res. Comm. v.204 Substitution of the ISP subunit of biphenyl dioxygenase from Pseudomonas results in a modification of the enzyme activity Tan, H.-M.;C.-M. Cheong
  21. J. Indust. Microbiol. Biotechnol. v.19 Aromatic hycrocarbon degradation by Sphingomonas yanoikuyae B1 Zylstra, G.J.;E. Kim