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Cloning and Sequence Analysis of Spinach (Spinacia oleracea L. cv Ace) Nitrate Reductase cDNA  

Park, Nu-Ri (Department of Agricultural Chemistry, Daegu University)
Chung, Jong-Bae (Department of Agricultural Chemistry, Daegu University)
Park, Sang-Gyu (Department of Agricultural Chemistry, Daegu University)
Publication Information
Applied Biological Chemistry / v.45, no.3, 2002 , pp. 129-133 More about this Journal
Abstract
Suppression of nitrate accumulation in spinach and lettuce through foliar application of chitosan formula containing micronutrients is related with the increase of the nitrate reductase (NR) activity. If NR in spinach were highly expressed to increase the assimilatory activity, nitrate content could be reduced. For this, NR cDNA was cloned from the isolated mRNAs of spinach using reverse transcriptase-PCR. Nucleotide sequence of cloned spinach NR cDNA showed highly deduced amino acid sequence identity ($71{\sim}82%$) with other known plant NR genes. Only two nucleotide-base differences were observed in the cloned NR cDNA compared with that of the published spinach NR cDNA.
Keywords
spinach; nitrate reductase; cDNA; RT-PCR;
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