• Proceedings of the KIPE Conference
• /
• 2003.07a
• /
• pp.439-445
• /
• 2003

Since 1960s. container shipping volume has increased dramatically and continuous on a trend of rapid growth, and so the number of containers handled at the port increases. In order to improve yard crane operating efficiency, the precise position measurement of the yard crane is important. This paper describes the method to measure the absolute position of yard crane using the output pulse of an encoder and infrared sensors. The crane position is calculated by counting the output pulse of an incremental encoder, which is mounted on the wheel in the crane. By the way, the wheel slippage on rail may cause some errors in crane position information obtained from encoder pulses, and the errors in the crane position information are compensated with infrared sensors. The performance of proposed method is verified on experimental results with the simulator of yard crane, the size of which is about 1/10 with the real crane.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.9 no.2
• /
• pp.163-170
• /
• 2004

Since 1960s, a container shipping volume has increased dramatically and continuous on a trend of rapid growth, and so the number of containers handled at the port increases. The position measurement of the yard crane is very important for improving the operating efficiency of the port. This paper describes the method to measure the absolute position of yard crane accurately and rapidly, using both the output pulse of an encoder and infrared sensors. The crane position is calculated by counting the output pulse of an incremental encoder, which is mounted on the wheel in the crane. By the way, the wheel slippage on rail may cause some errors in the crane position information obtained from encoder pulse, and the infrared sensor is used to compensate for errors in the crane position information. The performance of proposed method is verified on experimental results with the simulator of yard crane, the size of which is about 1/10 with the real crane.

• Speech Sciences
• /
• v.11 no.2
• /
• pp.105-117
• /
• 2004

Recently, many scholars have emphasized the importance of English listening ability for smoother communication. Most audio materials, however, were recorded in a quiet sound-proof booth. Therefore, students who have spent so much time listening to the ideal audio materials are expected to have difficulty communicating with native speakers in the real life. In this study, we examined how well thirty three Korean university students and five native speakers will repeat the recorded English sentences under noise and speed conditions. The subjects' production was scored by listening to each recorded sentence and counting the number of words correctly produced and determined the percent ratios of correctly produced words to the total words in each sentence. Results showed that the student group correctly repeated around 65% of all the words in each sentence while the native speakers demonstrated almost perfect match. It seemed that the students had difficulty perceiving and repeating function words in various conditions. Also, high-proficiency student group outperformed the low-proficiency student group particularly in their repetition of function words. In addition, the student subjects' accuracy of repetition remarkably dropped when the normal sentences were both sped up and mixed with noise. Finally, it was observed that the Korean students' percent correct ratio fell down as the stimulus sentence became longer.

• Journal of Microbiology and Biotechnology
• /
• v.12 no.5
• /
• pp.801-806
• /
• 2002

A multiplex competitive polymerase chain reaction (PCR) method was developed for the rapid identification and quantification of Leuconostoc mesnteroides and Lactobacillus plantarum populations which are the key microorganisms in kimchi fermentation. The strain-specific primers were designed to selectively amplify the target genes encoding 165 rRNA of L. plantarum and dextransucrase of L. mesenteroides. There was a linear relationship between the band intensity of PCR products and the number of colony forming units of each model organism. The PCR quantification method was compared with a traditional plate-counting method f3r the enumeration of the two lactic acid bacteria in a mixed suspension culture and also applied to a real food system, namely, watery kimchi. The population dynamics of the two model organisms in the mixed culture were reliably predictable by the competitive PCR analysis.

• Journal of Sensor Science and Technology
• /
• v.11 no.1
• /
• pp.28-38
• /
• 2002

People are important targets for video surveillance and monitoring(VSAM) but difficult to be analyzed. In this paper, a technique to count people in image sequences is dealt as a prerequisite procedure for automatic tracking and behaviour analysis. A group of people is divided at local minima of the line connecting the highest pixels on the binary image of the people extracted from the image taken by a stationary video camera. As the properties of the divided regions vary according to the relative positions of the people in a group, different states are assigned for the completely occluded, partially occluded, completed separated individual, and wrongly divided regions. By analyzing the transition of the states of divided regions, the number of people on the site monitored is estimated. The technique is checked in real experimental situations.

• The Journal of Internal Korean Medicine
• /
• v.40 no.1
• /
• pp.58-69
• /
• 2019

Objective: This study investigated the effects of Hansu-Daebowon (HDW) on bone resorption in vitro and bone loss in vivo. Methods: Osteoclast differentiation was measured by counting TRAP (+) MNC formed from RAW 264.7 in the presence of RANKL. Bone pit formation was determined in an artificial bone slice loaded with RANKL-stimulated osteoclasts. To elucidate the mechanisms of the inhibitory effects of HDW on bone resorption and osteoclast differentiation, osteoclastogenic genes (i.e. TRAP, MMP-9, NFATc1, c-Fos, and Cathepsin K) were measured using real time PCR. Furthermore, bone loss was observed using micro-CT in an LPS-treated mammal model. Results: HDW inhibited the bone pit formation in vitro and inhibited bone loss in vivo. Moreover, HDW decreased the number of TRAP (+) MNCs in the presence of RANKL, and HDW inhibited the expressions of cathepsin K, MMP-9, TRAP, NFATc1, and c-Fos in the osteoclasts. Conclusion: HDW exerts inhibitory effects on bone loss and bone resorption resulting from the inhibitions of osteoclast differentiation and osteoclastogenic gene expression.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.26 no.3
• /
• pp.36-53
• /
• 2013

Objectives : The purpose of this study is to investigate the effects of DOGO phreatic water containing sulphur on Atopic Dermatitis in NC/Nga mouse. Methods : We made DOGO phreatic water removed sulphur using Twin Alternating Sulfate Eater. After making atopic dermatitis caused by sensitizing NC/Nga mouse to DNCB(dinitrochlorobenzene), we made mouse swim in tanks each filled with distilled water, tap water, DOGO phreatic water(contain sulphur), DOGO phreatic water(remove sulphur) for 30minutes everyday. 3weeks later, we analyzed skin clinical score, total IgE levels(by ELISA), WBC differential counting(Neutrophils, Monocytes), absolute cell number of $Neutrophil^+Gr-1^+$, CCR3 mRNA expressions(by Real-time PCR), IL-4, IFN-${\gamma}$ production levels(by ELISA), histologic test(by H&E staining, toluidine blue staining). Results : The results of making NC/Nga mouse induced atopic dermatitis swim in tanks filled with DOGO phreatic water(contain sulphur) are as follows. 1. Skin clinical scores were decreased significantly in comparison to control group. 2. Total IgG levels were decreased significantly in comparison to control group. 3. WBC differential counting(Neutrophils, Monocytes) were decreased significantly in c.mparison to control group. 4. Absolute cell number of $Neutrophil^+Gr-1^+$ were decreased significantly in comparison to control group. 5. CCR3 mRNA expressions were decreased significantly in comparison to control group. 6. IL-4, IFN-${\gamma}$ production levels were decreased significantly in comparison to control group. 7. The epithelial tissue thickness, leucocytes infiltration, erythema, edema, excoriation, scaling, mast cells infiltrations in dorsal skin were decreased in comparison to control group. Conclusions : These results indicate that DOGO phreatic water(contain sulphur) can be used for helping treat atopic dermatitis.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.27 no.4
• /
• pp.141-157
• /
• 2014

Objectives : The purpose of this study is to investigate the effects of DOGO phreatic water containing germanium on Atopic Dermatitis in NC/Nga mouse. Methods : We made DOGO phreatic water added germanium. After making atopic dermatitis caused by sensitizing NC/Nga mouse to DNCB(dinitrochlorobenzene), we made mouse swim in tanks each filled with distilled water, tap water, DOGO phreatic water, DOGO phreatic water(added germanium) for 30minutes everyday. 3weeks later, we analyzed skin clinical score, total IgE levels(by ELISA), WBC differential counting(Neutrophils, Monocytes), absolute cell number of $Neutrophil^+Gr-1^+$, CCR3 mRNA expressions(by Real-time PCR), IL-4, IFN-${\gamma}$ production levels(by ELISA), histologic test(by H&E staining, toluidine blue staining). Results : The results of making NC/Nga mouse induced atopic dermatitis swim in tanks filled with DOGO phreatic water(contain germanium) are as follows. 1. Skin clinical scores were decreased significantly in comparison to control group. 2. Total IgG levels were decreased significantly in comparison to control group. 3. WBC differential counting(Neutrophils, Monocytes) were decreased significantly in comparison to control group. 4. Absolute cell number of $Neutrophil^+Gr-1^+$ were decreased significantly in comparison to control group. 5. CCR3 mRNA expressions were decreased significantly in comparison to control group. 6. IL-4, IFN-${\gamma}$ production levels were decreased significantly in comparison to control group. 7. The epithelial tissue thickness, leucocytes infiltration, erythema, edema, excoriation, scaling, mast cells infiltrations in dorsal skin were decreased in comparison to control group. Conclusions : These results indicate that DOGO phreatic water(contain germanium) can be used for helping treat atopic dermatitis.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.13 no.5
• /
• pp.1014-1021
• /
• 2009

In an environment of home network where a number of technologies including heterogeneous hardware platforms, networking and protocols, middleware systems, and etc, exist, OSGi provides a platform for deployment and sharing of services managed in hardware and guarantees compatibility among applications. However, only simple control and processing of event data are considered in a home network using OSGi, and the consideration about real time processing of data stream generated by sensors is not enough. Therefore, researches allowing users to effectively develop OSGi applications by using various kinds of sensors generating data streams in the home network environment using OSGi are needed. In this paper, we propose an effective method of processing various types of real time data streams supplied to OSGi applications, including filtering, grouping, and counting, etc.

• IMMUNE NETWORK
• /
• v.10 no.1
• /
• pp.15-25
• /
• 2010

Background: Rat mast cells were regarded as a good model for mast cell function in immune response. Methods: Rat bone marrow mast cells (BMMC) were prepared both by recombinant rat IL-3 (rrIL-3) and by recombinant mouse stem cell factor (rmSCF), and investigated for both proliferation and differentiation in time course. Rat BMMC was induced by culture of rat bone marrow cells (BMCs) in the presence of both rrIL-3 (5 ng/ml) and rmSCF (5 ng/ml). Culture media were changed 2 times per week with the cell number condition of $5{\times}10^4/ml$ in 6 well plate. Proliferation was analyzed by cell number and cell counting kit-8 (CCK-8) and differentiation was by rat mast cell protease (RMCP) II and histamine. Results: Cell proliferation rates reached a maximum at 8 or 11 days of culture and decreased thereafter. However, both RMCP II production and histamine synthesis peaked after 11 days of culture. By real time RT-PCR, the level of histidine decarboxylase mRNA was more than 500 times higher on culture day 11 than on culture day 5. By transmission electron microscopy, the cells were heterogeneous in size and contained cytoplasmic granules. Using gated flow cytometry, we showed that cultured BMCs expressed high levels of $Fc{\varepsilon}RI$ and the mast cell antigen, ganglioside, on culture day 11. Conclusion: These results indicate that rat BMMCs were generated by culturing BMCs in the presence of rrII-3 and rmSCF and that the BMMCs have the characteristics of mucosal mast cells.