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http://dx.doi.org/10.22246/jikm.2019.40.1.58

Effects of Hansu-Daebowon (HDW) on RANKL-induced Osteoclast Differentiation and Bone Loss in Mammal Model  

Jang, Si-sung (Gachon University College of Korean Medicine)
Ryu, Hong-sun (Hanibonga Research Center)
Jeon, Chan-yong (Dept. of Korean Internal Medicine, Gachon University Gil Oriental Medical Hospital)
Hwang, Gwi-seo (Gachon University College of Korean Medicine)
Publication Information
The Journal of Internal Korean Medicine / v.40, no.1, 2019 , pp. 58-69 More about this Journal
Abstract
Objective: This study investigated the effects of Hansu-Daebowon (HDW) on bone resorption in vitro and bone loss in vivo. Methods: Osteoclast differentiation was measured by counting TRAP (+) MNC formed from RAW 264.7 in the presence of RANKL. Bone pit formation was determined in an artificial bone slice loaded with RANKL-stimulated osteoclasts. To elucidate the mechanisms of the inhibitory effects of HDW on bone resorption and osteoclast differentiation, osteoclastogenic genes (i.e. TRAP, MMP-9, NFATc1, c-Fos, and Cathepsin K) were measured using real time PCR. Furthermore, bone loss was observed using micro-CT in an LPS-treated mammal model. Results: HDW inhibited the bone pit formation in vitro and inhibited bone loss in vivo. Moreover, HDW decreased the number of TRAP (+) MNCs in the presence of RANKL, and HDW inhibited the expressions of cathepsin K, MMP-9, TRAP, NFATc1, and c-Fos in the osteoclasts. Conclusion: HDW exerts inhibitory effects on bone loss and bone resorption resulting from the inhibitions of osteoclast differentiation and osteoclastogenic gene expression.
Keywords
Hansu-Daebowon (HDW); osteoclast differentiation; TRAP; cathepsin K; NFATc1; bone loss; bone resorption;
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