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A Study on Classification System for Gong-Po-Do Style in Tomb Wall Paintings of Koguryo (고구려 고분벽화 공포도 형식의 분류체계에 관한 연구)

  • Hwang, Se-Ok
    • Korean Journal of Heritage: History & Science
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    • v.49 no.2
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    • pp.20-55
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    • 2016
  • Koguryo's tomb mural paintings in North Korea are our precious cultural heritage which have been designated as UNESCO World Heritage property receiving high praise in the following criterion, i) exceptional creativeness of human being, ii) representative value showing the stage of development in construction history of East-Asia, iii) aesthetic superiority iv) uniqueness of building construction including tombs' ceiling. Mural paintings have been found from almost 100 tombs of the Koguryo dynasty out of 130 which are scattered across Huanren County, Lianoning Province, Ji'an, Jilin Province in China and Pyongyang in North Korea. Especially, most of them are gathered in Pyongyang from 4th and 5th century. Peculiarly, some of them have been constructed before King Jangsu's transfer of the capital to Pyongyang(AD 427). It can be regarded that Pyongyang territory had been under control of Koguryo and to become a new capital in the near future. And dense emergence of such tombs since the capital transfer from Gungnae City to Pyongyang during the reign of Jangsu is linked closely to the construction of tombs for rulers under strengthen royal authority of Jangsu and centralized system of authoritarian rule. Tomb mural paintings describe the owner's figure pictorially based on the truth just as in his living years. General lifestyles of ruling powers and sovereigns can be seen from the wall paintings portraying several buildings with various styles, figures, manners of living, which are considered that the tomb owner had led politically and sociologically in his life. In spite of not enough proofs to approve figure of architectures or "Gong-Po" in wall paintings on the tombs as those of Koguryo, it is persuasive with consideration for painting and decoration inside the tomb like wooden building in real life for the purpose of reenacting and continuing the tomb owner's luxurious life after death. "Du-Gong-Po-Zak" had appeared in company with Koguryo tomb murals and it can be found in most of the murals. And the emergence of substantial "Gong-Po-Do" can be counted more than a century ahead of the figure in murals. It could be a reasonable assumption as regards Koguryo tomb murals time of appearance match up with production period of Gahyungmyunggi(家形明器) and Hwasangseok(畵像石) Hwasangjeon(畵像塼) Design in the Mural Painting of the East-Han(東漢) Ancient Tombs in China. On this study, architectural "Gong-Po"s described in Koguryo tomb murals are categorized largely in "Bi(non)-Po-Zak-kye", "Jun(semi)-Po-Zak-kye", and "Po-Zak-kye" based on presence of "Ju-Du", "Cheom-Cha", and "Cheom-Cha-Sal-Mi" with developmental aspect, and, "Po-zak" is subdivided as "Bi(non)-Cheul-Mok" and "Cheul-Mok" types.

Analysis of Heat Transfer Characteristics on Multi-layer Insulating Curtains Coated with Silica Aerogel (실리카 에어로겔이 흡착된 다겹보온커튼의 전열 특성 분석)

  • Jin, Byung-Ok;Kim, Hyung-Kweon;Ryou, Young-Sun;Lee, Tae-Seok;Kim, Young-Hwa;Oh, Sung-Sik;Kang, Geum-Choon
    • Journal of Bio-Environment Control
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    • v.28 no.3
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    • pp.273-278
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    • 2019
  • The multi-layer insulating curtains used in the experiment was produced in six combinations using non-woven fabric containing aerogel and compared and analyzed by measuring heat flux and heat perfusion rates due to weight, thickness and temperature changes. Using silica aerogel, which have recently been noted as new material insulation, this study tries to produce a new combination of multi-layer insulating curtains that can complement the shortcomings of the multi-layer insulating curtains currently in use and maintain and improve its warmth, and analyze the thermal properties. The heat flux means the amount of heat passing per unit time per unit area, and the higher the value, the more heat passing through the multi-layer insulating curtain, and it can be judged that the heat retention is low. The weight and thickness of multi-layer insulation curtains were found to be highly correlated with thermal insulation. In particular, insulation curtains combined with aerogel meltblown non-woven fabric had relatively higher thermal insulation than insulation curtains with the same number of insulation materials. However, the aerogel meltblown non-woven fabric is weak in light resistance and durability, and there is a problem that the production process and aerogel are scattering. In order to solve this problems, the combination of expanded aerogel non-woven fabric and hollow fiber non-woven fabric, which are relatively simple manufacturing processes and excellent warmth, are suitable for use in real farms.

Validation of Stem-loop RT-qPCR Method on the Pharmacokinetic Analysis of siRNA Therapeutics (Stem-loop RT-qPCR 분석법을 이용한 siRNA 치료제의 생체시료 분석법 검증 및 약물 동태학적 분석)

  • Kim, Hye Jeong;Kim, Taek Min;Kim, Hong Joong;Jung, Hun Soon;Lee, Seung Ho
    • Journal of Life Science
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    • v.29 no.6
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    • pp.653-661
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    • 2019
  • The first small interfering RNA (siRNA) therapeutics have recently been approved by the Food and Drug Administration in the U.S., and the demand for a new RNA therapeutics bioanalysis method-which is essential for pharmacokinetics, including the absorption, distribution, metabolism, and excretion of siRNA therapeutics-is rapidly increasing. The stem-loop real-time qPCR (RT-qPCR) assay is a useful molecular technique for the identification and quantification of small RNA (e.g., micro RNA and siRNA) and can be applied for the bioanalysis of siRNA therapeutics. When the anti-HPV E6/E7 siRNA therapeutic was used in preclinical trials, the established stem-loop RT-qPCR assay was validated. The limit of detection was sensitive up to 10 fM and the lower limit of quantification up to 100 fM. In fact, the reliability of the established test method was further validated in three intra assays. Here, the correlation coefficient of $R^2$>0.99, the slope of -3.10 ~ -3.40, and the recovery rate within ${\pm}20%$ of the siRNA standard curve confirm its excellent robustness. Finally, the circulation profiles of siRNAs were demonstrated in rat serum, and the pharmacokinetic properties of the anti-HPV E6/E7 siRNA therapeutic were characterized using a stem-loop RT-qPCR assay. Therefore, the stemloop RT-qPCR assay enables accurate, precise, and sensitive siRNA duplex quantification and is suitable for the quantification of small RNA therapeutics using small volumes of biological samples.

Effects of high-fat diet induced obesity on tissue zinc concentrations and zinc transporter expressions in mice (고지방식이로 유도한 비만이 마우스의 조직 아연 농도와 아연수송체 발현에 미치는 영향)

  • Min, Byulchorong;Chung, Jayong
    • Journal of Nutrition and Health
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    • v.51 no.6
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    • pp.489-497
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    • 2018
  • Purpose: Obesity is often associated with disturbances in the mineral metabolism. The purpose of this study was to investigate the effects of high-fat diet-induced obesity on tissue zinc concentrations and zinc transporter expressions in mice. Methods: C57BL/6J male mice were fed either a control diet (10% energy from fat, control group) or a high-fat diet (45% energy from fat, obese group) for 15 weeks. The zinc concentrations in the serum, stool, and various tissues were measured by inductively coupled plasma (ICP)-atomic emission spectrophotometry or ICP-mass spectrophotometry. The levels of zinc transporter mRNAs in the liver, duodenum, and pancreas were measured by real-time RT-PCR. The levels of serum adipokines, such as leptin and IL-6, were determined. Results: The total body weight, adipose tissue weight, and hepatic TG and cholesterol concentrations were significantly higher in the obese group, as compared to the control group. The obese group had significantly higher levels of serum leptin and pro-inflammatory IL-6 concentrations, and had significantly lower levels of serum alkaline phosphatase activity. The zinc concentrations of the liver, kidney, duodenum, and pancreas were all significantly lower in the obese group than in the control group. On the other hand, the fecal zinc concentrations were significantly higher in the obese group than in the control group. The serum zinc concentrations were not significantly different between the two groups. The ZnT1 mRNA levels of the liver and the pancreas were significantly higher in the obese group, as compared to the control group. Hepatic Zip10 mRNA was also increased in the obese group. Conclusion: Our study findings suggest that obesity increases fecal zinc excretion and lowers the tissue zinc concentrations, which may be associated with alterations in the zinc transporter expressions.

Epidemiology and Clinical Characteristics of Parainfluenza Virus Type 4 in Korean Children: a Single Center Study, 2015-2017 (소아에서 파라인플루엔자 바이러스 4형의 역학 및 임상 양상에 대한 단일기관 연구: 2015-2017)

  • Sohn, Young Joo;Choi, Youn Young;Yun, Ki Wook;Choi, Eun Hwa;Lee, Hoan Jong
    • Pediatric Infection and Vaccine
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    • v.25 no.3
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    • pp.156-164
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    • 2018
  • Purpose: We aimed to identify the epidemiology and the clinical characteristics of human parainfluenza virus type 4 (HPIV-4) infection compared to HPIVs 1-3 infections in Korean children. Methods: We reviewed medical records of children with HPIV infection who visited Seoul National University Children's Hospital from 2015 to 2017. Detection of respiratory viruses was performed using real time-polymerase chain reaction (rt-PCR), which could differentiate HPIVs 1-4. Diagnosis was classified as a febrile illness, upper respiratory tract infection (URI), croup, bronchiolitis, or pneumonia. The epidemiology, demographic features, and clinical characteristics among HPIV types were compared. The clinical data were analyzed only for the previously healthy children. Results: Of the 472 children diagnosed with HPIV infection, 108 (22.9%) were previously healthy: 24 (22.2%), 19 (17.6%), 39 (36.1%), and 26 (24.1%) in HPIV types 1, 2, 3, and 4, respectively. The median age of children with HPIV-4 infection was 11 (0-195) months: the proportion of children aged < 2 years and 2 to < 5 years were 65.4% and 19.2%, respectively. Clinical diagnoses of HPIV-4 infection were bronchiolitis (38.5%), pneumonia (30.8%), and URI (30.8%). Croup was the most prevalent in HPIV-2 (21.1%) and none in HPIV-4 infection (P=0.026). Hospital admission rates among HPIV types were not significantly different (P>0.05). Conclusions: We observed seasonal peak of HPIV-4 infection in 2015 and 2017. HPIV-4 was a common respiratory pathogen causing lower respiratory tract infection in hospitalized children.

Anomaly Detection for User Action with Generative Adversarial Networks (적대적 생성 모델을 활용한 사용자 행위 이상 탐지 방법)

  • Choi, Nam woong;Kim, Wooju
    • Journal of Intelligence and Information Systems
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    • v.25 no.3
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    • pp.43-62
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    • 2019
  • At one time, the anomaly detection sector dominated the method of determining whether there was an abnormality based on the statistics derived from specific data. This methodology was possible because the dimension of the data was simple in the past, so the classical statistical method could work effectively. However, as the characteristics of data have changed complexly in the era of big data, it has become more difficult to accurately analyze and predict the data that occurs throughout the industry in the conventional way. Therefore, SVM and Decision Tree based supervised learning algorithms were used. However, there is peculiarity that supervised learning based model can only accurately predict the test data, when the number of classes is equal to the number of normal classes and most of the data generated in the industry has unbalanced data class. Therefore, the predicted results are not always valid when supervised learning model is applied. In order to overcome these drawbacks, many studies now use the unsupervised learning-based model that is not influenced by class distribution, such as autoencoder or generative adversarial networks. In this paper, we propose a method to detect anomalies using generative adversarial networks. AnoGAN, introduced in the study of Thomas et al (2017), is a classification model that performs abnormal detection of medical images. It was composed of a Convolution Neural Net and was used in the field of detection. On the other hand, sequencing data abnormality detection using generative adversarial network is a lack of research papers compared to image data. Of course, in Li et al (2018), a study by Li et al (LSTM), a type of recurrent neural network, has proposed a model to classify the abnormities of numerical sequence data, but it has not been used for categorical sequence data, as well as feature matching method applied by salans et al.(2016). So it suggests that there are a number of studies to be tried on in the ideal classification of sequence data through a generative adversarial Network. In order to learn the sequence data, the structure of the generative adversarial networks is composed of LSTM, and the 2 stacked-LSTM of the generator is composed of 32-dim hidden unit layers and 64-dim hidden unit layers. The LSTM of the discriminator consists of 64-dim hidden unit layer were used. In the process of deriving abnormal scores from existing paper of Anomaly Detection for Sequence data, entropy values of probability of actual data are used in the process of deriving abnormal scores. but in this paper, as mentioned earlier, abnormal scores have been derived by using feature matching techniques. In addition, the process of optimizing latent variables was designed with LSTM to improve model performance. The modified form of generative adversarial model was more accurate in all experiments than the autoencoder in terms of precision and was approximately 7% higher in accuracy. In terms of Robustness, Generative adversarial networks also performed better than autoencoder. Because generative adversarial networks can learn data distribution from real categorical sequence data, Unaffected by a single normal data. But autoencoder is not. Result of Robustness test showed that he accuracy of the autocoder was 92%, the accuracy of the hostile neural network was 96%, and in terms of sensitivity, the autocoder was 40% and the hostile neural network was 51%. In this paper, experiments have also been conducted to show how much performance changes due to differences in the optimization structure of potential variables. As a result, the level of 1% was improved in terms of sensitivity. These results suggest that it presented a new perspective on optimizing latent variable that were relatively insignificant.

Using Viable Eggs to Determine Oviposition Models and Life Table Analysis of Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) (톱다리개미허리노린재의 수정란을 이용한 산란모형과 생명표분석)

  • Ahn, Jeong Joon;Choi, Kyoung San;Koh, Sang Wook
    • Korean journal of applied entomology
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    • v.58 no.2
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    • pp.111-120
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    • 2019
  • Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) is an economically important insect pest of soybean and fruit trees. We investigated the temperature effects on the adult fecundity and longevity, and determined the parameters of oviposition models and life table at different constant temperatures 15.8, 19.7, 24.0, 27.8, 32.6, 34.0, and $35.5^{\circ}C$. R. pedestris females reproduced successfully from 19.7 to $35.5^{\circ}C$ except $15.8^{\circ}C$. The longevity of R. pedestris was longest at $15.8^{\circ}C$ and it decreased with increasing temperature (76.6 days at $19.7^{\circ}C$ and 20.6 days at $35.5^{\circ}C$). The number of total eggs and viable eggs was highest at $24.0^{\circ}C$ (193.5 and 151.2). Egg hatchability was highest at $27.8^{\circ}C$ (84.0%). We compared the results of oviposition models and life table parameters using both total eggs and viable eggs. The parameter value (c: the maximum reproductive capacity) (190 eggs) of temperature dependent total fecundity model using total eggs was higher than that of the model using viable eggs. When we analyzed the life table parameter the values of net reproductive rate and mean generation time using viable eggs were lower than those using total eggs. The oviposition models and life table analysis using viable eggs will be helpful to understand the real population transition of R. pedestris in agricultural system.

Establishment of Geospatial Schemes Based on Topo-Climatology for Farm-Specific Agrometeorological Information (농장맞춤형 농업기상정보 생산을 위한 소기후 모형 구축)

  • Kim, Dae-Jun;Kim, Soo-Ock;Kim, Jin-Hee;Yun, Eun-Jeong
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.3
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    • pp.146-157
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    • 2019
  • One of the most distinctive features of the South Korean rural environment is that the variation of weather or climate is large even within a small area due to complex terrains. The Geospatial Schemes based on Topo-Climatology (GSTP) was developed to simulate such variations effectively. In the present study, we reviewed the progress of the geospatial schemes for production of farm-scale agricultural weather data. Efforts have been made to improve the GSTP since 2000s. The schemes were used to provide climate information based on the current normal year and future climate scenarios at a landscape scale. The digital climate maps for the normal year include the maps of the monthly minimum temperature, maximum temperature, precipitation, and solar radiation in the past 30 years at 30 m or 270 m spatial resolution. Based on these digital climate maps, future climate change scenario maps were also produced at the high spatial resolution. These maps have been used for climate change impact assessment at the field scale by reprocessing them and transforming them into various forms. In the 2010s, the GSTP model was used to produce information for farm-specific weather conditions and weather forecast data on a landscape scale. The microclimate models of which the GSTP model consists have been improved to provide detailed weather condition data based on daily weather observation data in recent development. Using such daily data, the Early warning service for agrometeorological hazard has been developed to provide weather forecasts in real-time by processing a digital forecast and mid-term weather forecast data (KMA) at 30 m spatial resolution. Currently, daily minimum temperature, maximum temperature, precipitation, solar radiation quantity, and the duration of sunshine are forecasted as detailed weather conditions and forecast information. Moreover, based on farm-specific past-current-future weather information, growth information for various crops and agrometeorological disaster forecasts have been produced.

A Study on the Characteristics of PM1.0 Chemical Components Using a Real-time Aerosol Mass Spectrometer (실시간 에어로졸 질량분석기를 이용한 PM1.0의 화학적성분의 특성에 관한 연구)

  • Park, Jinsoo;Choi, Jinsoo;Kim, Hyunjae;Oh, Jun;Sung, Minyoung;Ahn, Joonyoung;Lee, Sangbo;Kim, Jeongho
    • Journal of the Korean Society of Urban Environment
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    • v.18 no.4
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    • pp.485-494
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    • 2018
  • This study aims to identify the characteristics of oxidation and chemical composition of PM in winter season, 2017 at Incheon area. The mean concentration of air pollutants were $46{\pm}22{\mu}g/m^3-PM_{10}$, $29{\pm}18{\mu}g/m^3/-PM_{2.5}$, $5{\pm}3ppb-SO_2$, $0.56{\pm}0.24ppm-CO$, $21{\pm}13ppb-O_3$ and $28{\pm}17ppb-NO_2$, respectively. The dominant ion of the $PM_{1.0}$ chemical component were organic with $3.2{\mu}g/m^3$ and nitrate with $1.9{\mu}g/m^3$. The day and night variation of the $PM_{1.0}$ chemical components was higher in nighttime than those of daytime. The averaged nitrate oxidation rate (SOR) was 0.06 and sulfate oxidation rate was 0.11 during the field campaign. In the high mass loading period, nitrate oxidation rate (NOR) was up to 0.6 and also the nitrate in $PM_{1.0}$ was increased. The averaged ratio of $NO_x/SO_2$ was 8.7 and nitrate/sulfate was 3.1, respectively. In this results, the nitrate component in $PM_{1.0}$ was influenced by NOx from the stationary source as power plant and the mobile source around the measurement site.

Alteration of MicroRNAs Targeted Integrins by PD-MSCs Transplantation Is Involved in Hepatic Regeneration in a Rat Model with BDL (담관결찰 쥐 모델에서 태반유래중간엽줄기세포 이식에 의한 miRNA 표적 인테그린 변화의 간재생 효과)

  • Park, Sohae
    • Journal of Life Science
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    • v.31 no.8
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    • pp.710-718
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    • 2021
  • Placenta-derived mesenchymal stem cells (PD-MSCs) are promising candidates for cell-based therapy in regenerative medicine. The migration and homing potential of PD-MSCs to injured sites is a critical property of MSC engraftment. MicroRNAs (miRNAs) have recently been shown to regulate the critical functions of MSCs, such as proliferation, survival, and migration. The objective of the present study was to identify the miRNA and target genes involved in PD-MSCs homing in a bile duct ligation (BDL) rat model. We selected candidate miRNAs targeting genes for PD-MSCs homing based on microarray analysis. PD-MSC engraftment in BDL-injured rat liver was identified by immunofluorescence assay and human-specific Alu gene expression by quantitative real-time polymerase chain reaction (qRT-PCR) one week after transplantation. Compared with migrated naïve PD-MSCs under hypoxic and normoxic conditions (Hyp/Nor), the transplanted group with PD-MSCs (Tx) showed distinct differences in miRNA expressions in BDL-injured rat liver. We also validated the miRNAs and their target genes for PD-MSCs homing. The expressions of integrin α4 (ITGA4) and integrin α5 (ITGA5) target genes for miR-199a-5p and miR-148a-3p were significantly upregulated in the Tx group (p<0.05). In addition, integrin β1 (ITGB1) and integrin β8 (ITGB8) were upregulated by suppressing miR-183-5p and miR-145-5p, respectively. These results demonstrated that PD-MSCs regulate miRNA expression related to the integrin family for their homing effects on the BDL-injured rat liver. The findings further suggest that miRNA-mediated regulation of the integrin family contributes to the therapeutic efficacy of PD-MSCs in the rat hepatic fibrosis model by BDL.