• Journal of Microbiology and Biotechnology
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• 제20권12호
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• pp.1653-1663
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• 2010

The first gene (${\alpha}$-gal1) encoding an extracellular ${\alpha}$-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized. The ${\alpha}$-gal1 gene consisted of an open reading frame of 1,792 base pairs interrupted by six introns that encoded a mature protein of 452 amino acids, including a 24 amino acid secretory signal sequence. The translated protein had highest identity with other fungal ${\alpha}$-galactosidases belonging to glycosyl hydrolase family 27. The ${\alpha}$-gal1 gene was overexpressed as a secretory protein with an N-terminal histidine tag in the methylotrophic yeast Pichia pastoris. Recombinant ${\alpha}$-Gal1 was secreted into the culture medium as a monomeric glycoprotein with a maximal yield of 10.75 mg/l and purified to homogeneity using Hisbinding nickel-agarose affinity chromatography. The purified enzyme was maximally active at $70^{\circ}C$, pH 4.5, and lost no activity over 10 days at $50^{\circ}C$. ${\alpha}$-Gal1 followed Michaelis-Menten kinetics ($V_{max}\;of\;240.3{\mu}M/min/mg,\;K_m\;of\;0.294 mM$) and was inhibited competitively by galactose ($K_m{^{obs}}$ of 0.57 mM, $K_i$ of 2.77 mM). The recombinant T. emersonii ${\alpha}$-galactosidase displayed broad substrate preference, being active on both oligo- and polymeric substrates, yet had strict specificity for the ${\alpha}$-galactosidic linkage. Owing to its substrate preference and noteworthy stability, ${\alpha}$-Gal1 is of particular interest for possible biotechnological applications involving the processing of plant materials.

• 한국게임학회 논문지
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• 제14권5호
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• pp.15-24
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• 2014

기호학은 인간의 삶과 관련된 모든 기호의 구조와 기호의 체계를 연구하는 학문이므로 게임을 분석하기에 충분하다. '게임을 한다'는 것은 기호학적으로 보아 게임을 읽는 것, 즉 해석하는 행위로 볼 수 있다. 따라서 게임을 만든 제작자는 '발신자', 게임은 '텍스트', 게이머는 '수신자' 그리고 게이머가 게임을 플레이하는 것은 '컨텍스트'로 볼 수 있다. 그러나 게임 대부분은 다변수적 서사형태를 기본으로 하기 때문에 게이머가 제작자의 의도대로 게임을 해석하는 경우는 많지 않다. 이에 본고는 제작자의 의도와 게이머의 반응이 동일한 콘솔게임 (2001)를 분석대상으로 삼고 기호학적 해석을 시도하였다. 분석방법으로는 줄거리 분석, 시퀀스 분석을 통해 이야기 프로그램을 정의하였고, 의미론적 분석을 위해 행동자 모델, 기호 사각형 모델을 적용 하였다. 이 과정에서 대결, 도움, 협조, 지배의 코드를 찾을 수 있었으며 비주체적인 인물이었던 이코와 요르다가 주체적 인물로 변화됨을 확인할 수 있었다. 즉, 는 비주체적 삶을 살던 주인공들이 삶을 획득하는 이야기라고 해석할 수 있다.

• 대한지리학회지
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• 제44권3호
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• pp.282-300
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• 2009

이 논문은 서울의 도심을 근대성과 후기근대성이 공존하는 도시공간으로서의 문화텍스트로 상정하고 젠더문화연구의 관점에서 독해하기 위해 기획되었다. 물리적 공간과 사회적 주체 간의 관계가 어떻게 형성되어 왔는지를 주요 논제로 상던 기존의 도시사회학과 인문지리학적 논의를 배경으로 하여, 근대성이 관철되는 과정을 거치면서 형성된 서울이라는 도시 공간의 성별적 표상과 공간의 성별 분할을 파악하였다. 이미지성과 가독성을 중심으로 한 서울의 공간적 해석의 분석틀은 성별분업과 영역의 이분법, 여성성/남성성의 이분법적 재현. 그리고 근대성과 후기근대성이 이들과 조응하고 교차하는 방식 등으로 구성되었다. 이 논문에서는 Benjamin의 '산보하기(fl$\check{a}$nerie)' 의 도시문화 해석과 음미의 방식은 수용하되 이를 페미니스트 문화독해 방식으로 전화하여 은유적 방법론으로 차용하기 위하여 lrigaray의 '검경(speculum)으로 들여다보기'를 도입함으로써 도시공간분석의 새로운 해석적 방법론으로 제시하고자 시도하였다.

• 한국보육지원학회지
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• 제9권3호
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• pp.229-252
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• 2013

이 연구는 다문화 아동의 발달수준 및 어린이집 부적응행동 실태를 확인하기 위하여 경기도 김포시와 안산시에 소재한 34개 어린이집에 재원 중인 만 3-5세의 다문화 아동과 일반 아동 203명을 대상으로 WSS 발달적 체크리스트와 유아교육기관 생활적응행동검사를 실시하였다. 연구결과 다문화 아동이 일반 아동에 비하여 인성 및 사회성, 언어 및 읽고 쓰기 영역에서 발달수준이 낮은 것으로 나타났으나 수리적 사고와 과학적 사고 발달에는 차이가 없는 것으로 나타났다. 연령별로는 3세에서는 다문화 아동과 일반 아동 간 발달의 차이가 있는 것으로 나타났으나 4세와 5세에서는 차이가 없는 것으로 나타났다. 또한 다문화 아동과 일반 아동의 어린이집 부적응행동 수준에는 차이가 없는 것으로 밝혀졌다. 하위영역별로 어린이집 적응 수준을 살펴보면 다문화 아동이 일반 아동에 비하여 또래협력 항목에서는 적응수준이 낮게 나타났으나 개인사회기술과 환경적응, 활동적응 영역에서는 차이가 없는 것으로 나타났다. 아동의 발달과 어린이집 부적응행동의 관계를 살펴본 결과 아동의 발달 수준이 낮을수록 어린이집에 부적응하는 것으로 나타났으며, 발달 영역 중 '인성 및 사회성'영역의 발달이 아동의 어린이집 부적응행동을 가장 잘 예측해주는 변인인 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.728-732
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• 2007

In the present article, a novel fusion expression vector for Escherichia coli was developed based on the pTORG plasmid, a derivative of pET32a. This vector, named pT7MT(GenBank Accession No DQ504436), carries a T7 promoter and it drives the downstream gene encoding Metallothionein 2A(MT2A). There are in-framed multiple cloning sites(MCS) downstream of the MT2A gene. A target gene can be cloned into the MCS and fused to the C-terminal of the MT2A gene in a compatible open reading frame(ORF) to achieve fusion expression. The metal-binding capability of MT2A allows the purification of fusion proteins by metal chelating affinity chromatography, known as $Ni^{2+}$-affinity chromatography. Using this expression vector, we successfully got the stable and high-yield expression of MT2A-GST and MT2A-Troponin I fusion proteins. These two proteins were easily purified from the supernatant of cell lysates by one-step $Ni^{2+}$-affinity chromatography. The final yields of MT2A-GST and MT2A-Troponin I were 30mg/l and 28mg/l in LB culture, respectively. Taken together, our data suggest that pT7MT can be applied as a useful expression vector for stable and high-yield production of fusion proteins.

• 한국조경학회지
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• 제34권1호
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• pp.69-80
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• 2006

The purposes of this study are to analyze the meanings of urban spaces which are the background of the Korean films, and to capture the interactions of ordinary culture and urban spaces. By reading urban spaces through films, it is possible to understand the variety of experiences that are hardly captured with direct eyes, specific and vivid urban images, and various events formed by the interactions of spaces and people. The scope of the study is the urban spaces revealed in Korean films portraying cities after the 1960's as their settings, and the total of 18 films was selected with every $4{\sim}5$ films for each time stage. With the selected films, analytical meanings were developed with considering three aspects; 1) phylogenetic meaning that simply reflects social-cultural and historical background, 2) the outer strum meaning that concerns the situation of special background and film scene, and 3) metaphorical and metanymic meaning on films. According to the appearance frequency, spatial backgrounds of film scenes are mainly alleys, main streets, railroad, loft, and riverside. And then the connection between spaces and meaning clusters was grasped, and reflected meanings were derived for every spaces. And the meaning of urban space in films was analyzed based on the meaning of developments and outer stratum. The fundamental characteristics and feelings of people in media such as films are more emphasized than in real world. Urban space is not considered as a simple visible shell, but is recognized as 'a real situation' created by people. The intension of this study was to open the possibility of the various views of urban spaces. The construction of the urban space should be approached from a perspective of creating new places at where the space and human beings interact with considerations of stories of various human lives. I hope new vistas can be opened up for the research subjects and methodologies about the hereafter study of urban spaces through the mutual communications with various adjacent regions including films.

• 비교문화연구
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• 제38권
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• pp.265-295
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• 2015

This article is concerned about a reason for which Cervantes participates an arabic author named Benengeli and morisco translator in his work instead of christian author. From the multi-cultural point of view, the time in which Don Qujote was published, belongs to the Golden Age. In other words, the society can not be supported by the ideology of Purity of Blood in that the morisco, converso (Christian Jewish) have been permitted to coexist in the name of christian proselyte or New Christian despite of invisible discrimination. An invisible discrimination is based on the prejudice and negative stereotype of Old Christian against the New Christian. Cervantes offers an o open space for readers to participate in the creative reading, giving up the absolute authority of author named Benengeli. The deep-rooted prejudice against morisco or muslim author makes the readers of Don Quijote do reinterpret the contents and have question about his sincerity. This disbelief is partly on the basis of hypothesis that Don Quijote would be passed on orally by an arabic or morisco. Leaving the hypothesis alone, Romance, festival performances of morisco or the aljamia literature in the Iberian Peninsula have the chivalry or knights of the Occident. The chivalry in Romance of morisco means that morisco would seek assimilation into the mainstream of Occidental Christian community. At the same time, morisco would be faced with the dilemma of loss of religious identity. But Taqiyya, islamic doctrine, offsets the dilemma between yearning to assimilate into mainstream and religious conscience of morisco in that Taqiyya permits morisco to convert to Christianity in case that they are in danger of life or the following risk. From this point of view, There is no room for doubt about the fact that Taqiyya contributed to social assimilation or multicultural society of the Iberian Peninsula. It has been a long time since a narrow-minded religious dogma and ideology became a anachronistic relic in multicultural society of Spain such as the Purity of Blood. From a relative viewpoint, Don Quijote provides a ground for the collective intelligence among christian, muslim(morisco) and converso through a liberal community between readers and authors who form a pluralistic society.

• 비교문화연구
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• 제36권
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• pp.37-59
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• 2014

William Wordsworth's "The Thorn" revolves around the following questions: Who is Martha? Why does she go to the mountain top and repeat her doleful cry? To these questions, it gives us two different kinds of answers; one derives from the villagers, and the other from the narrator. This essay attempts to examine how the answers exemplify two different critical approaches to the problem of community, using Jacques Lacan's account of sexual difference in his seminar on Encore as a guiding thread of analysis. The important thing to retain here is that sexual difference in Lacan's seminar on Encore does not so much indicate biological determinations as two distinct forms of relating to the other which are intimately bound up with the question of how a community is constructed and maintained. The first form, called "masculine," suggests that it is a radical exception to a community that makes possible the community as a field of totality or sameness; the second form, called "feminine," shows that each of the subjects cannot be regarded as a member of a closed community which is guaranteed by the exceptionality, but as an exception that is radically singular. This in turn leads us to consider the possibility that the masculine form has to do with the villagers' effort to distinguish themselves from Martha and the feminine form with the way in which the narrator confronts and represents her. In the course of his formulation of sexuation graph, Lacan stresses that the masculine side must be supplemented by the feminine side, which allows us to elaborate on why, concerning Martha, the narrator does not just keep the completely different position from the villagers'. This is to say that the villagers' representation of Martha as an exception to the community should be supplemented by the narrator's attempt to tell Martha's story as the villagers do and at the same time to capture something of her enigmatic unrepresentability. Bearing in mind Charles Shepherdson's elaboration of traumatic memory, this essay also tries to clarify how the narrator preserves and even transmits something of Martha's truth that is embodied in her uncontrollable and unassimilable cry.

• 문화경제연구
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• 제21권2호
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• pp.111-139
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• 2018

이 연구는 인스타그램에 장문의 클래식음악 공연 리뷰를 게시하는 클래식음악 애호가에 주목하여, 공연 리뷰를 작성하는 동기와 목적을 밝히고 리뷰를 매개로 이루어지는 애호가들 사이의 상호 커뮤니케이션을 분석하였다. 분석 자료로는 인스타그램에 리뷰를 게시하는애호가 8인과 진행한 인터뷰를 주로 활용하였으며, 이들이 게시한 리뷰 글도 분석에 포함하였다. 분석 결과, 클래식음악 애호가들이 인스타그램을 애용하는 이유는 인스타그램의 용이성, 익명성, 친밀성의 세 가지 특성(어포던스) 때문인 것으로 파악되었다. 또한 이미지 중심의 인스타그램 운영 방식은 자신이 작성한 공연 리뷰 콘텐츠의 수준에 확신이 없는 애호가들에게 텍스트에 쏠리는 주목과 관심을 회피하는 효과를 가지는 것으로 나타났다. 즉, 이들은 클래식음악 감상에 적합한 문화자본을 가지지 못했다는 열등감 때문에 인스타그램에 리뷰를 게재하지만, 리뷰를 지속적으로 작성하고 읽는 과정에서 발생하는 애호가들 사이의 상호 학습을 통해 클래식음악 감상에 필요한 문화자본을 축적하고 있었다.

• Clinical and Experimental Pediatrics
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• 제64권11호
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• pp.575-581
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• 2021

Background: Toxocariasis is a zoonotic parasitic disease caused by Toxocara canis and Toxocara cati in humans. Various types of T. canis are important. Purpose: The current study aimed to investigate the prevalence of Toxocara spp. in pediatrics in the context of a systematic review and meta-analysis. Methods: The MEDLINE (PubMed), Web of Sciences, Embase, Google Scholar, Scopus, and Cumulative Index of Nursing and Allied Health databases were searched to identify peer-reviewed studies published between January 2000 and December 2019 that report the prevalence of Toxocara spp. in pediatrics. The evaluation of articles based on the inclusion and exclusion criteria was performed by 2 researchers individually. Results: The results of 31 relevant studies indicated that the prevalence of Toxocara spp. was 3%-79% in 10,676 cases. The pooled estimate of global prevalence of Toxocara spp. in pediatrics was 30 (95% confidence interval, 22%-37%; I²=99.11%; P=0.00). The prevalence was higher in Asian populations than in European, American, and African populations. Conclusion: Health policymakers should be more attentive to future research and approaches to Toxocara spp. and other zoonotic diseases to improve culture and identify socioeconomically important factors.