• Journal of Microbiology
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• v.33 no.3
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• pp.201-207
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• 1995

We have previously reported that SPP2 gene product of yeast Saccharomyces cerevisiae is involved in the pre-mRNA splicing. To investigate the rol ein the splicing pathway of the Spp2p protein, the SPP2 gene was overexpressed in Escherichia coli and polyclonal antibodies to Spp2p were generated from rabbits. First, a DNA fragment containing the SPP2 GENE without its promoter was subcloned into an E. coli expression vector, pKK233-3. The resulting recombinant plasmid pBQ14 contained an IPTG inducible tac promoter and the SPP2 structural gene. Overexpression of the SPP2 gene was achieved by additionof 0.1 to 1.0 mM IPTG to a logarithmic culture of E. coli JM103(pBQ14) for 90 min at 37.deg.C. Sequence of N-terminal 15 amino acids of the overproduced protein was well matched to the deduced one from the SPP2 reading frame. Then, polyclonal antibodies were generated from rabbits immunized with gel-purified SppSp protein. These antibodies reacted specifically with the Spp2p protein extracted from yeast cells expressing the SPP2 gene to a great extent. The antibodies could also block the activity of yeast splicing extracts.

• Journal of Korean language education
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• v.25 no.1
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• pp.115-144
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• 2014

The purpose of this paper is to compare the two different instruction methods for Korean learners of academic purposes in learning request expression. Participants were divided into two groups, explicit and implicit group. Both groups viewed several scenes from the drama that involved native speakers interacting in different situations. The instructional treatment for the explicit group included metapragmatic information while the treatment for the implicit group did not. On the other hand, the treatment for the implicit group followed implicit techniques, which were repetition of the video presentation and a script reading activity. This study was made up of a pre-test, a post-test, and a delayed-test. The pre-test was conducted prior to the instructional treatment. The post-test was administered a day after the last instruction and the delayed-test was conducted five weeks after the treatments. Two types of tests, speaking and writing, were used in this study to examine subjects' knowledge of Korean request. The result of this research reveals that implicit treatment was more effective than explicit treatment in Korean learners' request acquisition. This results might have been due to the operationalization of the implicit condition in this study. Implicit instruction may help language learners make rules by themselves through tasks.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.455-462
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• 2010

Purple acid phosphatase is important for phosphorus remobilization in plants, but its role in plant adaptation to low phosphorus availability is not known. The cDNA encoding O. sativa purple acid phosphatase (OsPAP1) has 1008 bp with an open reading frame of 335 amino acid residues. The amino acid sequence of OsPAP1 cDNA shows of 50-51% identity with other plant purple acid phosphatases. OsPAP1 was expressed in rice plants and in cell cultures in the absence of phosphate ($P_i$). The expression was organ-specific with the strongest expression in $P_i$-deprived roots. Functional expression of the OsPAP1 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analysis. OsPAP1 overexpression lines had higher phosphatase activity than wild-type. Overexpression of OsPAP1 in Arabidopsis plants resulted in increased Pi accumulation under Pi sufficient condition. These results show that the OsPAP1 gene represents more efficient $P_i$ uptake and can be used to develop new transgenic dicotyledonous plants.

• Mycobiology
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• v.46 no.4
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• pp.429-439
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• 2018

To develop a convenient promoter analysis system for fungi, a null-pigment mutant (NPG) of Aspergillus nidulans was used with the 4'-phosphopantetheinyl transferase (PPTase) gene, npgA, which restores the normal pigmentation in A. nidulans, as a new reporter gene. The functional organization of serially deleted promoter regions of the A. nidulans trpC gene and the Cryphonectria parasitica crp gene in filamentous fungi was representatively investigated to establish a novel fungal promoter assay system that depends on color complementation of the NPG mutant with the PPTase npgA gene. Several promoter regions of the trpC and crp genes were fused to the npgA gene containing the 1,034-bp open reading frame and the 966-bp 3' downstream region from the TAA, and the constructed fusions were introduced into the NPG mutant in A. nidulans to evaluate color recovery due to the transcriptional activity of the sequence elements. Serial deletion of the trpC and crp promoter regions in this PPTase reporter assay system reaffirmed results in previous reports by using the fungal transformation step without a laborious verification process. This approach suggests a more rapid and convenient system than conventional analyses for fungal gene expression studies.

• Microbiology and Biotechnology Letters
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• v.47 no.1
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• pp.139-147
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• 2019

Glutamate decarboxylase catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), contributing to pH homeostasis through proton consumption. The reaction is the first step toward the GABA shunt. To date, the enzymes involved in the glutamate metabolism of Photobacterium damselae subsp. piscicida have not been elucidated. In this study, an open reading frame of P. damselae subsp. piscicida, showing homology to the glutamate decarboxylase or putative pyridoxal-dependent aspartate 1-decarboxylase genes, was isolated and cloned into an expression vector to produce the recombinant enzyme. Preliminary gas chromatography-mass spectrometry characterization of the purified recombinant enzyme revealed that it catalyzed not only the decarboxylation of glutamate but also the transamination of GABA. This enzyme of P. damselae subsp. piscicida could be bifunctional, combining decarboxylase and transaminase activities in a single polypeptide chain.

• English & American cultural studies
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• v.9 no.1
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• pp.297-321
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• 2009

The purpose of this study is to re-read the film of Dead Poets Society, specially focused on a feminist view. The film hides the strategy of recovering the traditional Patriarchal Society. At the beginning, the film resists the values of traditional society through John Keating. His unorthodox methods of teaching literature smack against the traditions of Welton Academy. Furthermore, he stresses on "Carpe Diem"—Seize the Day, the romantic values of free thinking, creativity, and individuality. The forces opposing Keating's philosophy are personified by Welton's rigid, old headmaster, Mr. Nolan, and the cruel, stubborn parent, Mr. Perry. Keating's romantic values are failed by their powerful, dominating attitudes. Effected by Keating's philosophy, Neil decides to pursue acting rather than medicine. He conflicts with his strict father. Finally frustrated by his authority, Neil commits suicide. And Keating is accused of inciting the boys to restart the Dead Poets Society, and at last he is fired. Keating and Neil are victimized by the Patriarchal society. Even though the film concentrates male characters at the all boys' school, it reveals the male angle of binary oppositions between men and women, subject and object, activity and passivity, presence and absence. In the film's dramatic conclusion, English class is now being temporarily taught by Nolan, who has the boys read from the very Pritchard essay they had ripped out at the start of the film. It symbolizes the triumph of the traditional logocentric society. However, influenced by Keating's unconventional attitudes, ultimately Welton Academy will be changed as it is embodied in its closing scene.

• International Journal of Computer Science & Network Security
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• v.21 no.3
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• pp.76-82
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• 2021

An anthological analysis of known literature and historical sources is carried out in the work. It was found that the development of foreign language training of future professionals was influenced by a number of factors: socio-economic (focus on the needs of the labor market, integration into the international space, scientific and technological progress); educational (updating legal documents in the field of education, standardization of educational content, development of methods of professional development of a specialist). The historical period is analyzed and the following stages are determined: ideological (realization of ideological imperative in language and professional training of future specialists; educational-methodical (preparation according to unified curricula, reading and translation as a leading type of speech activity); integration (integration of foreign language teaching and multicultural education)), methodological (use of traditional verbal methods, standardized textbooks). Thus, the research conducted in the article indicates the periods (stages) of formation, functioning and development of foreign language education.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.35-42
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• 2021

The college English teaching-learning model in this study is intended to effectively apply dictogloss activities to enhance competencies such as communication, self-directedness, and cooperation by upgrading the utilization of various online platform functions. Dictogloss is a language teaching and learning activity that combines four functions (listening, speaking, reading, and writing) of communication. College English classes in this study focus on communication-oriented integrated English education. In this study, the teaching and learning is an online-based English integrated teaching-learning method based on constructivism theory. The model presented the roles of learners and teachers according to the seven procedures.

• Nuclear Engineering and Technology
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• v.54 no.12
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• pp.4644-4651
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• 2022

Due to its short half-life, thoron gas has been assumed to have negligible health hazards on humans compared to radon. But, one of the decay products with a long half-life can make it to be transported to a long distance and to cause a severe internal dose through respiration. Since most commercial radon detectors can not discriminate thoron signals from radon signals, it is very common to overestimate radon doses which in turn result in biased estimation of lung cancer risk in epidemiological studies. Though some methods had been suggested to measure thoron and radon separately, they could not be used for real-time measurement because of CR-39 or LR-115. In this study, an effective method was suggested to measure radon and thoron separately from the free air. It was observed that the activity of thoron decreases exponentially due to delay time caused by a long pipe between two chambers. Therefore from two ion chambers apart in time, it was demonstrated that thoron and radon could be measured separately and simultaneously. We also developed a collimated alpha source and with this source and an SBD, we could convert the ion chamber reading to count rate in cps.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.20 no.2
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• pp.197-208
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• 2009

The Purpose of the study is to analyze present state of the volunteers in cultural program activities in Korean libraries and for reinforcing library volunteer activity. 2,349 volunteers were asked questionaires for the purpose looking into present state. The factors were number of regional participants, libraries which have partaken, number of participants per program, and participating programs. The programs that volunteers have partaken, its kind, relative importance of the partaken program, "non-reading" program's contents, preferable program by each targeted users, and the most participated program were analyzed.