• Title/Summary/Keyword: Reactive black 5

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Isolation of Anthocyanin from Black Rice (Heugjinjubyeo) and Screening of its Antioxidant Activities (흑미(현진주벼)유래 안토시아닌의 항산화능 탐색)

  • Park, Young-Sam;Kim, Sun-Joong;Chang, Hyo-Ihl
    • Microbiology and Biotechnology Letters
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    • v.36 no.1
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    • pp.55-60
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    • 2008
  • Colored rices are a hulled grains having red or purple pigments in bran. Especially black rice (Heugjinjubyeo) is considered to be a healthy food in Asia. Black rice is of great interesting because of the possible biological activity with their anthocyanins. Anthocyanins are water-soluble plant pigments and representatives of flavonoids. The anthocyanins in black rice include cyanidin 3-O-glucoside, peonidin 3-O-glucoside, malvidin 3-O-glucoside, pelagonidin 3-O-glucoside and delphinidin 3-O-glucoside. In this study, anthocyanins in a black rice were analyzed quantitatively and qualitatively with HPLC and UV-Vis spectrophotometer. The anthocyanins contained approximately 95% of cyanidin-3-O-glucoside and 5% of peonidin-3-O-glucoside. Antioxidant activities of the anthocyanin extract were investigated by using various in vitro methods. The 100g/ml concentration of the anthocyanin extracted exhibited 88.83% inhibition on the peroxidation of linoleic acid, 55.20% DPPH free radical scavenging activity, 54.96% superoxide anion radical scavenging activity, and 72.67% hydrogen peroxide scavenging activity. And it also showed high ferrous ion reducing capability. These results suggest that the anthocyanin extracted from black rice may be utilized as a possible antioxdiant agent against ROS.

Preparation and Optical Properties of Polarizing Film Based on Poly(vinyl Alcohol) Dyed by Reactive Dichroic Dyes Using Organic Solvents (유기 용매를 사용한 반응성 이색성 염료의 염착에 의한 폴리비닐알코올계 편광필름의 제조 및 광학특성)

  • Choi, E-Joon;Choi, Seung Sock;Kim, Eun-Chol;Kim, Si Min;Back, Sang-Hyun
    • Journal of Adhesion and Interface
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    • v.13 no.3
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    • pp.131-136
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    • 2012
  • In this study, commercial poly(vinyl alcohol) (PVA) film was dyed with reactive dichroic dyes under mild conditions using organic solvents in stead of strong basic aqueous solution. After drawing of 500% of this PVA film, the polarizing efficiency and the single piece transmittance were measured. The degree of saponification of the commercialized PVA film was determined by using NMR and FT-IR spectromety. The commercial PVA film, with ca. 100% of the degree of saponification determined by NMR spectrometry, was dyed with the reactive dichroic dyes, which have 3,5-dichloro-2,4,6-triazine moiety. As a result, we found that the PVA film dyed with the reactive congo red showed relatively good polarization efficiency, and the PVA film dyed with the reactive direct black 22 exhibited relatively good single piece transmittance.

The Quality Characteristics of Boiled Pork Supplemented with Tea Extracts (녹차, 오룡차, 홍차 추출물을 첨가한 돼지고기 수육의 품질특성)

  • Cho, Kyung Ok;Kim, Sun Im
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.5
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    • pp.774-783
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    • 2013
  • This study investigated the quality characteristics of boiled pork with different amounts (0.5, 1, 1.5, or 2%) of green tea, oolong tea and black tea extracts. Characteristics measured included approximate composition, water holding capacity (WHC), hardness, pH, Hunter's color value, total aerobic bacterial counts, volatile basic nitrogen (VBN), 2-thiobarbituric acid reactive substances (TBARS), and sensory qualities. Green tea approximately contained 3.4%, 31.8%, 5.5%, and 57.7% of moisture, crude protein, crude ash, and carbohydrate, respectively. There were no significant differences with the type of tea. However, the crude fat content of green tea, oolong tea, and black tea were 1.6%, 1.0%, and 0.9%, respectively. Green tea had the highest content of crude fat (p<0.05). Boiled pork approximately contained 55.1%, 38.6%, 5.2% and 0.9% moisture, crude protein, crude fat and crude ash, respectively. The WHC of boiled pork, with tea extract added, significantly increased and there were no significant differences according to the type of tea. The hardness of boiled pork significantly increased as the amount of tea extract added increased (p<0.05). The pH of the boiled pork was not significantly different after storage for one day, but significantly decreased in control groups and boiled pork with 0.5% of any tea extract added during storage. However, in boiled pork with 1%, 1.5%, or 2% of any tea extract added, pH was not significantly different during storage. The Hunter's L and b values decreased in all boiled pork. The Hunter's a-values also decreased in boiled pork with green and black tea extract added, but increased in boiled pork with oolong tea extract added. In boiled pork with tea extract added, total aerobic bacterial counts significantly decreased as the amount of tea extract added increased during storage (p<0.05). The VBN values significantly increased during storage in all groups. TBARS values were significantly lower in boiled pork with green tea extract added, oolong tea added (at concentrations of 1%, 1.5%, or 2%), and black tea extract added compared to control groups on the first day. The sensory evaluation results showed that the color, flavor, and overall acceptance of boiled pork containing 1% of oolong or black tea extracts had the highest scores but there were no significant differences. However, taste scores were significantly different (p<0.05). These results indicate that boiled pork has improved quality characteristics with 1% of oolong or black tea extracts added.

Effect of Pre-treatment Agents on the Digital Textile Printing of Silk Fabrics (견직물의 디지털 프린팅에 있어 전처리제가 염색성에 미치는 영향)

  • Lee, San-Ha;Jeong, Dong-Seok;Chun, Tae-Il
    • Textile Coloration and Finishing
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    • v.23 no.4
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    • pp.263-273
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    • 2011
  • Digital textile printing(DTP) technology made considerable advances in recent years. In this study, a pre-treatment agent has been prepared for the better coloration of digital textile printing. The ink formulation contained three kinds of 5g thickener (CMC, Sodium alginate, Dextrin), 25g urea, 5g sodium carbonate, and 465g distilled water. The optimal sharpness of outline was found in the 1-3% concentration of the pre-treating agent with a viscosity of 10-15 cSt. Even if the color difference between untreated and treated samples was not apparent in the printing step, the color appearance increased after steaming. The color appearance of cyan, magenta, yellow, black reactive colorants increased in the order of CMC>Sodium alginate>Dextrin. Wash fastness to shade change and staining for the treated samples were 4-5 rating, while untreated sample was 1-2 rating. Also, the pre-treated sample with 1:1 mixtures had 4-5 rating. Both dry and wet rubbing fastness to shade change and staining were excellent in the treated samples, whereas rubbing fastness of untreated sample was 1-2 rating. With exception of 3 rating to black color, light fastness properties were 4 rating for the remaining three colors in the regardless of treatment condition and mixing of pre-treating agents. Dry cleaning fastness of all samples were also 4-5 rating irrespective of treatment condition and mixing of pre-treating agents.

Comparison of Functional Properties of Blood Plasma Collected from Black Goat and Hanwoo Cattle

  • Shine Htet Aung;Edirisinghe Dewage Nalaka Sandun Abeyrathne;Mahabbat Ali;Dong Uk Ahn;Young-Sun Choi;Ki-Chang Nam
    • Food Science of Animal Resources
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    • v.43 no.1
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    • pp.46-60
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    • 2023
  • Slaughterhouse blood is a by-product of animal slaughter that can be a good source of animal protein. This research purposed to examine the functional qualities of the blood plasma from Hanwoo cattle, black goat, and their hydrolysates. Part of the plasma was hydrolyzed with proteolytic enzymes (Bacillus protease, papain, thermolysin, elastase, and α-chymotrypsin) to yield bioactive peptides under optimum conditions. The levels of hydrolysates were evaluated by 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis. The antioxidant, metal-chelating, and angiotensin I-converting enzyme (ACE) inhibitory properties of intact blood plasma and selected hydrolysates were investigated. Accordingly, two plasma hydrolysates by protease (pH 6.5/55℃/3 h) and thermolysin (pH 7.5/37℃/3-6 h) were selected for analysis of their functional properties. In the oil model system, only goat blood plasma had lower levels of thiobarbituric acid reactive substances than the control. The diphenyl picrylhydrazyl radical scavenging activity was higher in cattle and goat plasma than in proteolytic hydrolysates. Ironchelating activities increased after proteolytic degradation except for protease-treated cattle blood. Copper-chelating activity was excellent in all test samples except for the original bovine plasma. As for ACE inhibition, only non-hydrolyzed goat plasma and its hydrolysates by thermolysin showed ACE inhibitory activity (9.86±5.03% and 21.77±3.74%). In conclusion, goat plasma without hydrolyzation and its hydrolysates can be a good source of bioactive compounds with functional characteristics, whereas cattle plasma has a relatively low value. Further studies on the molecular structure of these compounds are needed with more suitable enzyme combinations.

The Antioxidative Effect of Black Garlic Extract on Paraquat-induced Oxidative Stress in ICR Mice (Paraquat에 의해 유발된 산화적 스트레스에 의한 흑마늘 추출물의 항산화 효과)

  • Noh, Byung-Kyu;Lee, Jung-Kyu;Won, Yong-Duk;Park, Hyun-Jin;Lee, Sung-Joon
    • Korean Journal of Food Science and Technology
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    • v.43 no.6
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    • pp.760-765
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    • 2011
  • We investigated the antioxidative effect of black garlic extract (BGE) on paraquat (PQ)- induced oxidative stress in mice. A DPPH radical scavenging activity assay showed that BGE had potent free radical scavenging activity, comparable to that exhibited by vitamin C. Mice were administered with either vitamin C or two levels of BGE by oral gavage for 10 days, with PQ being injected intraperitoneally on day five. It was found that BGE reduced the liver enzyme levels induced by PQ injection in mice. The concentrations of plasma and hepatic malonedialdehyde were both significantly reduced in the BGE groups compared with the levels in the PQ group, whereas the hepatic superoxide dismutase and catalase activities were significantly increased in the BGE groups compared with the PQ group. These findings suggest that BGE has potent antioxidative activities in vivo and thus could prevent the oxidative stress induced by PQ injection in mice by two mechanisms: the induction of antixoxidative enzyme systems and direct scavenging of reactive oxygen species.

A study on shelf life of prepackaged retail-ready Korean native black pork belly and shoulder butt slices during refrigerated display

  • Hoa, Van-Ba;Seol, Kuk-Hwan;Kang, Sun-Moon;Kim, Yun-Seok;Cho, Soo-Hyun
    • Animal Bioscience
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    • v.34 no.12
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    • pp.2012-2022
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    • 2021
  • Objective: In most retail centers, primal pork cuts for sale are usually prepared into retail-ready slices and overwrapped with air-permeable plastic film. Also, meat of Korean native black pig (KNP) is reputed for its superior quality, however, its shelf life during retail display has not been studied. Thus, the objective of this study was to evaluate shelf life of prepackaged retail-ready KNP belly and shoulder butt slices during refrigerated display. Methods: Bellies and shoulder butt obtained at 24 h post-mortem from finishing KNP were used. Each belly or shoulder butt was manually cut into 1.5 cm-thick slices. The slices in each cut type were randomly taken and placed on white foam tray (2 slices/tray) overwrapped with polyvinyl chloride film. The retail-ready packages were then placed in a retail display cabinet at 4℃. Shelf life and sensory quality of the samples were evaluated on day 1, 3, 6, 9, 12, and 15 of display. Results: The shoulder butt reached the upper limit (20 mg/100 g) of volatile basic nitrogen for fresh meat after 9 days while, the belly remained within this limit throughout the display time (15 days). Both the cuts reached a thiobarbituric acid reactive substances level of above 0.5 mg malondialdehyde/kg after 9 days. The a* (redness) values remained unchanged during first 9 days in both cuts (p>0.05). After 9 days, off-flavor was not found in either cut, but higher off-flavor intensity was found in shoulder butt after 12 days. The shoulder butt was unacceptable for overall eating quality after 12 days while, belly still was acceptable after 12 days. Conclusion: The belly showed a longer shelf life compared to the shoulder butt, and a shelf life of 9 and 12 days is recommended for the prepackaged retail-ready KNP shoulder butt and belly slices, respectively.

Effect of Nutrients on the Production of Extracellular Enzymes for Decolorization of Reactive Blue 19 and Reactive Black 5

  • Lee Yu-Ri;Park Chul-Hwan;Lee Byung-Hwan;Han Eun-Jung;Kim Tak-Hyun;Lee Jin-Won;Kim Sang-Yong
    • Journal of Microbiology and Biotechnology
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    • v.16 no.2
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    • pp.226-231
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    • 2006
  • Several white-rot fungi are able to produce extracellular lignin-degrading enzymes such as manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase. In order to enhance the production of laccase and MnP using Trametes versicolor KCTC 16781 in suspension culture, the effects of major medium ingredients, such as carbon and nitrogen sources, on the production of the enzymes were investigated. The decolorization mechanism in terms of biodegradation and biosorption was also investigated. Among the carbon sources used, glucose showed the highest potential for the production of laccase and MnP. Ammonium tartrate was a good nitrogen source for the enzyme production. No significant difference in the laccase production was observed, when glucose concentration was varied between 5 g/l and 30 g/l. As the concentration of nitrogen source increased, a lower MnP activity was observed. The optimal C/N ratio was 25 for the production of laccase and MnP. When the concentrations of glucose and ammonium tartrate were simultaneously increased, the laccase and MnP activities increased dramatically. The maximum laccase and MnP activities were 33.7 U/ml at 72 h and 475 U/ml at 96 h, respectively, in the optimal condition. In this condition, over 90% decolorization efficiency was observed.

In-situ functionalized biomass derived graphite-supported BiFeO3 for eradication of pollutants

  • Deepeka, Deepeka;Paramdeep, Kaur;Jyoti, Jyoti;Sandeep, Bansal;Sonal, Singhal
    • Advances in nano research
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    • v.13 no.6
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    • pp.527-543
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    • 2022
  • A novel, green, versatile and magnetically retrievable BiFeO3/CDR (Bismuth ferrite/coriander) nanocomposites were fabricated via simple wet chemical method utilizing in situ functionalized, cheap coriander seed powder (CDR 5%, 10%, 15% and 20 wt%) as a fuel to enhance the efficiency of pristine BiFeO3. A comparative study was performed between BiFeO3/CDR and BiFeO3/CNT (Bismuth ferrite/carbon nanotubes) nanocomposites for the removal of various hazardous pollutants from waste water. The successful synthesis of the fabricated nanomaterials was monitored via FT-IR, Powder XRD, FE-SEM, CV, VSM, CHNS/O and XPS studies. The synthesized nanomaterials were employed for the oxidative degradation of Carbol fuchsin, Reactive black 5, Ciprofloxacin and Doxorubicin; adsorption of a pesticide malathion; and reduction studies for Para-nitrophenol (PNP). The fabricated nanomaterials (BiFeO3/CDR) showcased excellent efficiency and comparable results with (BiFeO3/CNT) for the removal of model pollutants. Moreover, synthesized green heterojunction was also testified for mixture of textile and pharmaceutical waste. Hence CDR can be utilized as a better alternative of CNTs.

Quality Characteristics and Biological Activity of Fermented Black Garlic with Probiotics (Probiotics를 이용한 흑마늘 발효물의 품질특성 및 생리활성)

  • Tak, Hyun-Min;Kim, Gyeong-Min;Kim, Jong-Su;Hwang, Cho-Rong;Kang, Min-Jung;Shin, Jung-Hye
    • Journal of Life Science
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    • v.24 no.5
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    • pp.549-557
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    • 2014
  • This study examined the quality characteristics of fermented black garlic (BG) with probiotics. Nine strains of probiotics were tested in media containing 20% BG. Four of the strains grew well in the BG media: Lactobacillus rhamnosus, L. paracasei subsp. paracasei, L. casei, and L. plantarum. These four strains were used to make 10, 20, and 30% BG fermented product, respectively. The number of viable cells, pH, acidity, S-allyl cysteine (SAC) concentration, and nitric oxide (NO) and reactive nitrogen species (ROS) generation in Raw 264.7 macrophage cells were measured. L. plantarum showed the best growth of all the strains in the BG media. The pH of all the samples decreased during fermentation, and the acidity increased acidity. However, they did not differ significantly from the pH and acidity of the control. In all four strains, the SAC content did not differ before and after fermentation. However, the SAC content increased, depending on the BG concentration. NO production was inhibited in the L. rhamnosus inoculation strain compared to the other strains. ROS generation was also significantly inhibited in the L. plantarum inoculation strain compared to the other strains. The results show that the characteristics of BG fermentation products are determined by the fermentation strain. Therefore, fermentation products with particular characteristics can be produced using a single strain or mixed strains.