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http://dx.doi.org/10.5352/JLS.2014.24.5.549

Quality Characteristics and Biological Activity of Fermented Black Garlic with Probiotics  

Tak, Hyun-Min (Namhae Garlic Research Institute)
Kim, Gyeong-Min (Namhae Garlic Research Institute)
Kim, Jong-Su (Namhae Garlic Research Institute)
Hwang, Cho-Rong (Namhae Garlic Research Institute)
Kang, Min-Jung (Namhae Garlic Research Institute)
Shin, Jung-Hye (Namhae Garlic Research Institute)
Publication Information
Journal of Life Science / v.24, no.5, 2014 , pp. 549-557 More about this Journal
Abstract
This study examined the quality characteristics of fermented black garlic (BG) with probiotics. Nine strains of probiotics were tested in media containing 20% BG. Four of the strains grew well in the BG media: Lactobacillus rhamnosus, L. paracasei subsp. paracasei, L. casei, and L. plantarum. These four strains were used to make 10, 20, and 30% BG fermented product, respectively. The number of viable cells, pH, acidity, S-allyl cysteine (SAC) concentration, and nitric oxide (NO) and reactive nitrogen species (ROS) generation in Raw 264.7 macrophage cells were measured. L. plantarum showed the best growth of all the strains in the BG media. The pH of all the samples decreased during fermentation, and the acidity increased acidity. However, they did not differ significantly from the pH and acidity of the control. In all four strains, the SAC content did not differ before and after fermentation. However, the SAC content increased, depending on the BG concentration. NO production was inhibited in the L. rhamnosus inoculation strain compared to the other strains. ROS generation was also significantly inhibited in the L. plantarum inoculation strain compared to the other strains. The results show that the characteristics of BG fermentation products are determined by the fermentation strain. Therefore, fermentation products with particular characteristics can be produced using a single strain or mixed strains.
Keywords
Black garlic; immune activity; probiotics; reactive nitrogen species; S-allyl cysteine;
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