• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.445-449
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• 2003

As for the purpose, we first introduce an random mutation into wild-type gene to expand a mutation space, and then further recombine the mutant genes by staggered extension process PCR. As a result, we obtained the best clones 6-52 that showed a high activity and stability, from a round of error prone and staggered extension process PCR. The purified enzyme showed a similar pH stability to the wild-type enzyme and reveal a slightly high optimum pH at 12. In the optimum temperature, an identical dependency was also showed and a quite high stability in the thermal stability was obtained. Along with this, the enzyme was also stable at a reaction that supplement with a 15 % of ethanol as an additive. The addition of other solvents and surfactants did not improve the reaction and thus resulted in a similar profile to those of wild-type enzyme. The specific activity on the target compound rac-ketoprofen ethyl ester was calculated to be about 85, 000 unit, and the kinetic constants Km and Vmax were determined to be 0.2 mM and 90 mM/mg-protein/min respectively. The deduced amino acid alignment with the wild type enzyme revealed five mutations at L120P, I208V, T249A, D287H and T357A. Based on these observations, the site directed mutagenesis to delineate the mutagenic effect is under progress.

• Journal of Pharmaceutical Investigation
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• 제35권5호
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• pp.333-336
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• 2005

The objective of this study was to investigate the stability of tetracycline HCl on encapsulation into and inside reverse micelles. To do so, tetracycline HCl was first mixed with cetyltrimethylammonium bromide, water and ethyl formate to make reverse micelles. The degradation kinetics of tetracycline HCl inside the reverse micelles was then assessed by scrutinizing its stability data. Under our experimental conditions, the reverse micelles formed spontaneously in absence of any mixing devices. During the preparation of the reverse micelles, however, considerable portions of tetracycline HCl underwent a chemical reaction (e.g., epimerization). For instance, $51.4{\pm}0.6%$ of an initial concentration of tetracycline HCl was transformed into a degradation product. Once dissolved inside the reverse micelles, the degradation of tetracycline HCl followed an exponential decay pattern. The plot of log{the degradation rate of tetracycline HCl} versus log{tetracycline HCl concentration} made it possible to determine the order of degradation reaction and rate constant. It was proven that the degradation of tetracycline HCl inside the reverse micelles followed a first order kinetics with a rate constant of 0.0027 $hour^{-1}$. Meriting further investigation might be formulation studies to stabilize tetracycline HCl on encapsulation into and inside the reverse micelles.

• 약학회지
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• 제7권2_3호
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• pp.51-54
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• 1963

In this experiment, it is found that the decomposition reaction of hexamine aqueous solution by heat is the pseudo first order reaction and the calculated decomposition velocity constants of Hexamine aqueous solution are 1.17 * $10^{-5}min.^{-1}(60{\deg}$ C), 1.99 * $10^{-5}min.^{-1}(70{\deg}$ C), 2.35 * $10^{-5}min.^{-1}(80{\deg}$ C), 6.63 * $10^{-5}min.^{-1}(100{\deg}$ C). In the result, the activation energy of decomposition reaction of hexamine aqueous solution is 12 $Cal.mole^{-1}$.

• Journal of applied mathematics & informatics
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• 제26권1_2호
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• pp.365-374
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• 2008

We study a mathematical modeling for development of film negative and concentrate the bulk reaction problem. We prove nonnegativeness of developer, coupler and dye function in two dimensional case. Also we prove stability of our numerical scheme. Finally, we discuss numerical example which have specified constants.

• 대한수학회보
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• 제54권2호
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• pp.655-666
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• 2017

In this paper, we study the pattern formation to a general Degn-Harrison reaction model. We show Turing instability happens by analyzing the stability of the unique positive equilibrium with respect to the PDE model and the corresponding ODE model, which indicate the existence of the non-constant steady state solutions. We also show the existence periodic solutions of the PDE model and the ODE model by using Hopf bifurcation theory. Numerical simulations are presented to verify and illustrate the theoretical results.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.239.2-240
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• 2003

We have recently described the novel synthetic method for N-protected amines from various ethers using chlorosulfonyl isocyanate(CSI) and found that the mechanism of our CSI reaction is a competitive reaction of SN1 and SNi mechanism according to the stability of carbocation intermediate. Forthermore. we developed the regioselective and diastereoselective one-pot synthetic method for 1,2-amino alcohol, through the reaction of di-and tribenzyl thers with CSI, and invetigated its mechanism. (omitted)

• 대한수학회지
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• 제43권3호
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• pp.659-676
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• 2006

In this paper, we consider three-component reaction-diffusion system. With an integral condition and a global coupling, this system gives us an interesting free boundary problem. We shall examine the occurrence of a Hopf bifurcation and the stability of solutions as the global coupling constant varies. The main result is that a Hopf bifurcation occurs for global coupling and this motion is transferred to the stable motion for strong global coupling.

• 대한수학회보
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• 제49권6호
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• pp.1255-1262
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• 2012

A type of delayed Lotka-Volterra competition reaction-diffusion system is considered. By constructing a new Lyapunov function, we prove that the unique positive steady-state solution is globally asymptotically stable when interspecies competition is weaker than intraspecies competition. Moreover, we show that the stability property does not depend on the diffusion coefficients and time delays.

• International Journal of Automotive Technology
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• 제7권3호
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• pp.315-320
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• 2006

We have developed an active front steering system(AFS) with a planetary gear train, which can vary the steering gear ratio according to the vehicle speed and improve vehicle stability by superimposing steering angle. We conducted vehicle tests showing that co-operated control of AFS with ESP can improve vehicle stability by direct control of tire slip angle and that steering reaction torque during AFS intervention can be compensated by torque compensation using electric power steering.

• Bulletin of the Korean Chemical Society
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• 제22권2호
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• pp.179-182
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• 2001

Photocyclizable poly(cinnam-4'-yl methyl methacrylate) derivatives bearing methoxy benzene (PMCMMA), anthracene (PACMMA), and coumarin (PCCMMA) have been synthesized via Heck type reaction. Three different types of polymers are photoreactable usin g linearly polarized UV light and applicable as liquid crystal alignment layer. Anthracence and coumarin containing polymers (PACMMA, PCCMMA) have better thermal stability than PMCMMA. This observation may be attributed to the glass transition temperature elevation due to the bulky size and another photocrosslinking site provided by anthracene or coumarin group.