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Synthesis of Acetins from Glycerol using Lipase from Wheat Extract

  • Pradima, J;Rajeswari, M Kulkarni;Archna, Narula;Sravanthi, V;Rakshith, R;Nawal, Rabia Nizar
    • Korean Chemical Engineering Research
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    • v.57 no.4
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    • pp.501-506
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    • 2019
  • New technology-driven biocatalysts are revolutionizing the biochemical industries. With maximum utilization of renewable feedstock, biocatalysts have been the basis for a major breakthrough. Lipases are the most widely established catalysts used for hydrolysis, esterification and transesterification reactions. In this research, a biochemical process that combines extraction of lipase enzyme from germinated wheat seeds and its application to valorize glycerol to acetins by esterification is presented. Acetins are among highly rated, value-added products derived from glycerol. The favorable conditions for the enzymatic conversion of glycerol were observed as glycerol to acetic acid molar ratio (1:5), reaction temperature ($40^{\circ}C$) and the amount of enzyme (20% v/v). 65.93% of glycerol conversion was achieved for duration of 15 h with the use of tert-butanol solvent. This method proposes to explore the viability of a biological route to convert glycerol derived from biodiesel industry to acetins with further streamlining.

The Effect of Different Sources of Urease Enzyme on the Nutritive Value of Wheat Straw Treated with Urea as a Source of Ammonia

  • Khan, M.J.;Scaife, J.R.;Hovell, F.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.7
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    • pp.1063-1069
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    • 1999
  • Wheat straw samples (3-4 cm) were sprayed with solutions of urea (U) alone or with a dry addition of garden soil (GS), midden soil (MS), soya bean meal (SM) or jack bean meal (JM) as crude urease sources and with a pure urease (UR) enzyme. Each of the urease sources was included at two levels: 30 and 60 g/kg except pure urease, which was added at a level of 2.5 & 5.0 g/kg treated straw dry matter. Untreated straw without urease source was used as a control. After treatment, samples were sealed in polythene bags and stored for 2, 7, 14, 21 and 35 days at $19{^{\circ}C}$. The urease sources, their levels and treatment time produced significant effects on ammonia production (p<0.01). The addition of urease offered more flexibility in hydrolyzing urea in the shortest possible time. Incorporation of soya bean and jack bean meal was effective in reducing the modified acid detergent fiber (MADF) content of straw and the same time increasing organic matter (OM) digestibility. Overall effect, addition of soya bean to urea at a ratio of 1:1 appeared to be the most satisfactory urease source for the treatment of urea and wheat straw.

Application of a solid-phase fluorescence immunoassay to determine ampicillin residues in muscle tissue of olive flounder (Paralichthys olivaceus)

  • Jung, Won Chul;Ha, Ji Young;Chung, Hee Sik;Heo, Sung Hyeok;Kim, Suk;Lee, Hu Jang
    • Korean Journal of Veterinary Research
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    • v.46 no.3
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    • pp.291-294
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    • 2006
  • $Parallux^{TM}$, a solid-phase fluorescence immunoassay(SPFIA) developed for antibiotics residue detection in milk, was applied for analysis of fish muscle. The recommended therapeutic dose of ampicillin(100 mg/kg body weight, withdrawal period 7 days) was orally administered to a group of 25 olive flounders(Paralichthys olivaceus) for consecutive five days. Muscle was sampled after drug treatment 1st, 2nd, 3rd, 4th and 5th day. The concentration of ampicillin in muscle, determined by SPFIA, was compared with that of internal standard(10 ppb as ampicillin). The absorbance ratio of sample to internal standard(Bs/Bo) was employed as an index to determine the muscle residue in olive flounder. To investigate the recovery rate, the standard solutions were added to muscle samples to give final concentrations in muscle of 10 and 50 ng/ml. The recovery rates of all spiked samples were > 89% of the spiked value. Ampicillin was detected in muscle of fishes treated until the 3rd day of withdrawal period. The present study showed that the SPFIA can be easily adopted in predicting tissue residues for ampicillin in farmed fishes.

The Effects of Enterprise Resource Planning (ERP) Implementation on Corporate Performance (전사적 자원관리(ERP)의 도입효과에 관한 재무분석)

  • Lim, Chang-Woo;Lee, Seog-Hi
    • The Journal of Information Systems
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    • v.16 no.1
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    • pp.91-109
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    • 2007
  • This study investigates the extent to which the implementation of enterprise resource planning(ERP) systems enhance the corporate performance by examining financial variables of the implemented firms against those of the control fm. A total of 346 observations were obtained from 160 firms, 80 implemented firms and 80 control firms, for the period of 1998-2002. Results from the study partly support the assertion that ERP improves the full spectrum of business functions such as selling, marketing, purchasing, warehousing, accounting and human resources by tightly integrating enterprise-wise information databases. First the profitability of the implemented group was different from that of the control group. The indicator (dummy) variable was able to explain the differences in total cash flows between the implemented group fm and the control group firms. Second, ERP turned out In improve the efficiency in managing assets. The indicator variable has the power in explaining the differences in the amount of accounts receivables between the implemented group firms and the control group firms. Third, the product turnover ratio of the implemented group was different from that of the control group. Fourth, the average value added per employee and the net income per employee of the implemented group firms were greats- than those of the control group firms. Finally, the average magnitude of the administrative expenses of the implemented group firms was smaller than that of the control group firms.

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The effects of Zr on the mechanical workability in Cu-Ni-Mn-Sn connector alloys (커넥터용 Cu-Ni-Mn-Sn계 합금의 가공성에 미치는 Zr 첨가효과)

  • Han, Seung-Zeon;Kong, Man-Shik;Kim, Sang-Shik;Kim, Chang-Joo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.05b
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    • pp.246-249
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    • 2000
  • The effects of Zr on the mechanical workability and tensile strength of Cu-Ni-Mn-Sn-Al alloys have been investigated and the following results were obtained. The mechanical workability of Cu-Ni-Mn-Sn-Al alloys are increased with addition of Zr. And the surface cracks of specimen were not produced in Zr added Alloys. Especially in condition of hot-worked beyond the 90% working ratio, Zr contained specimen showed intra-granule crack propagation but Zr-free specimen showed inter-granule mode. The tensile strength have maximum value in 0.05% Zr contained alloy. The aging mechanism of Cu-Ni-Mn-Sn-Al alloys were varied by Zr addition.

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Application of a solid-phase fluorescence immunoassay to determine neomycin residues in muscle tissue of olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major)

  • Jung, Won Chul;Chung, Hee Sik;Shon, Ho Yeong;Lee, Hu-Jang
    • Korean Journal of Veterinary Research
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    • v.48 no.2
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    • pp.175-179
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    • 2008
  • Parallux, a solid-phase fluorescence immunoassay (SPFIA) developed for detection antibiotics residue in milk, was applied for analysis of antibiotics in muscle tissue of olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major). Fishes were dipped in neomycin 140 mg/ton water, the recommended therapeutic dose, for 24 h. Muscle samples were obtained on 1st, 2nd, 3rd, 4th and 5th day after drug treatment. The concentration of neomycin in muscle was determined using an internal standard (100 ppb as neomycin). The absorbance ratio of sample to internal standard (S/C) was employed as an index to determine the muscle residues in fishes. To investigate the recovery rate, the standard solutions were added to muscle samples to give final concentrations in muscle of 0.2 and 0.5 mg/ml. The recovery rates of all spiked samples were > 85% of the spiked value. Neomycin was detected in muscles of fishes treated after the 1st day of withdrawal period. On the 2nd day after drug treatment, all muscle samples showed negative reaction (S/C ration ${\leq}$ 1.0). The present study showed that the SPFIA can be applied for predicting residues of neomycin in muscle tissues of farmed fishes.

Effects of Phosphate Complex the Functional Properties of Fish Meat and Animal Meat of Mixture Pastes -Effect of Properties on the Quality Stability and Technical- (인산성 첨가에 따른 혼합 연육제품에 관한 연구 -품질 및 기능적 성질에 미치는 영향-)

  • 홍종만
    • The Korean Journal of Food And Nutrition
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    • v.3 no.1
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    • pp.39-56
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    • 1990
  • The effects of six kinds of phosphate complex on the water holding capacity (W.H.C) and protein solubility of hair tail, yellow tail runner and dried pollack meat paste were investigated and animal meat(pork, chicken and hare meat complex) The formulation of six kind of phosphate complex employed to this experiment were made by mixing several phosphate such as sodium polyphosphate, sodium pyrophosphate, sodium acid pyrophosphate, potassim pyrophosphate, sodium ultra-meta-phosphate, sodium-tetra-phosphate and monoglyceride at different mixture ratio Among the six kinds of phosphate complex, phosphate B complex which was formulated by mixing sodium polyphosphate 40%, sodium pyrophosphate 30%, sodium tetra mata phosphate 10%, sodium ultra meta phosphate 10% was most effective on enchanging the W H. C, and protein solubility of hair tail, yellow tail runner dried pollack meat past and in case of pork, chicken and hare meat paste. Phosphate C complex which was formulated by mixing sodium polyphosphate 50%. sodium pyrophosphate 30%, sodium tetra meta phosphate 10%, potassium pyrophosphate 10%, was more effective them other phosphate complex, and thief optimum addition level was 0.5% respectively in weight of fish meat paste. Texture characteristics such as hardness, cohesiveness and springiness value of Kamaboko(fish meat and pork, chicken, hare meat complex past meat product) were evaluted as best when 0.5% of Phosphate B complex was added The optimum cooking condition of Kamaboko to get good texture was heating for 20 minutes at 12$0^{\circ}C$.

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Electrochemical synthesis of nanosized hydroxyapatite/graphene composite powder

  • Vesna, Miskovic-Stankovic;Sanja, Erakovic;Ana, Jankovic;Maja, Vukasinovic-Sekulic;Miodrag, Mitric;Jung, Young Chan;Park, Soo Jin;Rhee, Kyong Yop
    • Carbon letters
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    • v.16 no.4
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    • pp.233-240
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    • 2015
  • Electrochemical synthesis was employed to prepare a novel hydroxyapatite/graphene (HAP/Gr) composite powder suitable for medical applications as a hard tissue implant (scaffold). The synthesis was performed in a homogeneous dispersion containing Na2H2EDTA·2H2O, NaH2PO4 and CaCl2 with a Ca/EDTA/PO43− concentration ratio of 0.25/0.25/0.15M, along with 0.01 wt% added graphene nanosheets, at a current density of 137 mA cm−2 and pH value of 9.0. The field emission scanning electron microscopy and transmission electron microscopy observations of the composite HAP/Gr powder indicated that nanosized hydroxyapatite particles were uniformly placed in the graphene overlay. Raman spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction confirmed graphene incorporation in the HAP/Gr powder. The electrochemically prepared HAP/Gr composite powder exhibited slight antibacterial effect against the growth of the bacterial strain Staphylococcus aureus.

Characterization of Carboxylated Cellulose Nanocrystals from Recycled Fiberboard Fibers Using Ammonium Persulfate Oxidation

  • KHANJANZADEH, Hossein;PARK, Byung-Dae
    • Journal of the Korean Wood Science and Technology
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    • v.48 no.2
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    • pp.231-244
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    • 2020
  • As a way of finding value-added materials from waste medium density fiberboard (MDF), this study characterized cellulose nanocrystals (CNCs) isolated by ammonium persulfate (APS) oxidation using recycled MDF fibers. Chemical composition of the recycled MDF fibers was done to quantify α-cellulose, hemicellulose, lignin, nitrogen, ash and extractives. The APS oxidation was performed at 60 ℃ for 16 h, followed by ultrasonication, which resulted in a CNC yield of 11%. Transmission electron microscope images showed that rod-like CNCs had an average length and diameter of 167±47 nm and 8.24±2.28 nm, respectively, which gave an aspect ratio of about 20. The conductometric titration of aqueous CNCs suspension resulted in a carboxyl content of 0.24 mmol/g and the degree of oxidation was 0.04. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy clearly showed the presence of carboxyl group on the CNCs prepared by the APS oxidation. The change of pH of the aqueous CNC suspension from 4 to 7 converted the carboxyl group to sodium carboxylate group. These results showed that the APS oxidation was facile and CNCs had a one-step preparation method, and thus suggested an optimization of the oxidation condition in future.

Study on the Waterproofing Performance of FGD Gypsum Building Products from Inorganic-Organic Composite Additives

  • Ding, Yi;Fang, Youchun;Ren, Qifang;Fang, Hui;Zhang, Qicai;Oh, Won-Chun
    • Korean Journal of Materials Research
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    • v.25 no.11
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    • pp.590-597
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    • 2015
  • In this article, poly methyl triethoxy silane was compounded with an inorganic waterproof admixture at a certain ratio to improve the performance of gypsum products; a new type of high-efficiency compound water-proofing additive was also investigated. Furthermore, the waterproof mechanism and the various properties on the hardened gypsum plaster were investigated in detail by XRD and SEM. The results show that the intenerate coefficient of gypsum plaster increased to more than 0.9; the water absorbing rate decreased to less than 10 %. Both the bending strength and the compressive strength of gypsum plaster increased by various degrees. The intenerate coefficient reached a maximum value of 0.73 and the strength of the samples showed almost no change when 5% cement alone was added. In this new type of the high-efficiency compound with waterproof additive, the optimal technological parameters for formulas were obtained to be: 5% cement, 18 % mineral powder, and 0.8% poly methyl triethoxy silane, to compound gypsum plaster. Meanwhile, the production of high performance gypsum as a building material has become possible.