Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Application of a solid-phase fluorescence immunoassay to determine ampicillin residues in muscle tissue of olive flounder (Paralichthys olivaceus)

  • Jung, Won Chul (College of Veterinary Medicine, Gyeongsang National University) ;
  • Ha, Ji Young (College of Veterinary Medicine, Gyeongsang National University) ;
  • Chung, Hee Sik (Livestock Division, Gyeongnam Provincial Government) ;
  • Heo, Sung Hyeok (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Suk (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu Jang (College of Veterinary Medicine, Gyeongsang National University)
  • Accepted : 2006.08.03
  • Published : 2006.09.30
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Abstract

$Parallux^{TM}$, a solid-phase fluorescence immunoassay(SPFIA) developed for antibiotics residue detection in milk, was applied for analysis of fish muscle. The recommended therapeutic dose of ampicillin(100 mg/kg body weight, withdrawal period 7 days) was orally administered to a group of 25 olive flounders(Paralichthys olivaceus) for consecutive five days. Muscle was sampled after drug treatment 1st, 2nd, 3rd, 4th and 5th day. The concentration of ampicillin in muscle, determined by SPFIA, was compared with that of internal standard(10 ppb as ampicillin). The absorbance ratio of sample to internal standard(Bs/Bo) was employed as an index to determine the muscle residue in olive flounder. To investigate the recovery rate, the standard solutions were added to muscle samples to give final concentrations in muscle of 10 and 50 ng/ml. The recovery rates of all spiked samples were > 89% of the spiked value. Ampicillin was detected in muscle of fishes treated until the 3rd day of withdrawal period. The present study showed that the SPFIA can be easily adopted in predicting tissue residues for ampicillin in farmed fishes.

Keywords

References

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