• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.151-151
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• 1993

A. 1. 중추신경계에서 일어나는 병변들, Alzheimer's disease, Parkinsonism 및 정상적인 노화현상들을 구명하는 연구의 일부분으로 뇌내의 신경세포들의 세포내물질의 변화를 관찰하고자, 이 실험을 시도하였다. 2. 중추신경계에 작용하는 각종 약물들의 작용기전, 그리고 부작용을 구명하여 그의 예방 및 치료방법을 모색하고자 한다. B. 1.Preparations: rat brain cytosol ＆ culture cell-line(NIE-115) 2. Method: NO or NOS의 생성함량을 직접측정 할 수 없으므로 간접방법을 택한다. [3Hlarginine 및 [3Hlguanine을 Preparation에 incubation시켜 일정한 시간 후 생성되는 [3H]citrulline 또는 cyclic GMP을 scintillation counter로 측정하여 그 함량으로 NO or NOS의 생성을 측정한다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.387.1-387.1
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• 2002

Aging and estrogen cleficiency after menopause induce bone loss and result in osteoporosis. This study was investigated effects of n-hexane fraction (Hx) extracted from Astragali Radix on osteoporosis with osteoblast-like cell line (MG-63 and Saos-2) and an ovariectomized (OVX) rat model. Proliferation of osteoblast-like cells. MG-63 and Saos-2. was tested with MTT and alkaline phosphatase (ALP) assays. (omitted)

• Food Science and Biotechnology
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• 제15권4호
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• pp.534-542
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• 2006

The effects of ethanol fractions of three different rice grain extracts, Jakwangchalbyeo, Hwasunchalbyeo, and Ilpumbyeo, on apoptotic cell death in the rat hepatoma H4IIE cell line were investigated using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell viability assay. One hundred mg/mL Jakwangchalbyeo extract significantly reduced cell viability to 69.5, 57.2, and 46.1% within 24, 48, and 72 hr, respectively. Fluorescence-activated cell sorting (FACS) analyses were also performed to characterize the cell death pattern caused by treatment with the rice grain extracts. Apoptotic cell death was clearly observed with time after treatment with the Jakwangchalbyeo extract. In Western blotting analysis, degradation of the 116 kDa poly-ADP-ribose polymerase (PARP) molecule was observed with concomitant formation of an 89 kDa product 24, 48, and 72 hr after treating cells with the Jakwangchalbyeo extract. This indicates that an apoptotic process caused cell death in these cells. In conclusion, red-pericarp Jakwangchalbyeo extract induced apoptotic cell death in H4IIE cells to a larger extent than the other rice extracts.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.169.2-170
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• 2003

Erythropietin (EPO), a hematopoietic factor is also required for normal brain development, and its receptor is localized in brain. Therefore, it is possible that EPO could act as a neurotropic factor inducing differentiation of neurons. The present study, we therefore investigated whether EPO can increase differentiation of undifferentiated cortical neuron isolated from postneonatal (Day 1) rat brains and PC12 cell, undifferentiated dopaminagic cell line. EPO dose (1-100 U/ml) dependently increased cell differentiation and expression of differentiation marker gene (neurofilament and tyrosine hydroxylase) in both cells. (omitted)

• 생약학회지
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• 제38권2호통권149호
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• pp.197-203
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• 2007

Benign prostatic hyperplasia (BPH) is one of the common disease in elderly men. Recently old-age population is increased and we are growing more and more interested in clinical importance of BPH. In this study, the effect of PLX, which was the mixture of tomato extract (including 2% of lycopene) and chitooligosaccharide, on prostatic cancer cell and testosterone-induced BPH in adult rats of the Sprague Dawley strain was determined. The cell viability was evaluated by MTT method using L929 and LNCaP cell line, pretreated with various concentrations of PLX. The expression of prostatic specific antigen (PSA) and 5${\alpha$}$-reductase genes were evaluated by realtime PCR using LNCaP cell line and compared various concentrations of PLX with 50 ${\mu}$M of finasteride. An experimental prostatic hyperplasia was induced in male Sprague Dawley rats by giving testosterone for 8 weeks. After 2 weeks from start of giving testosterone, PLX and finasteride were administered orally once a day. The results were analyzed with prostate weight per body weight at 8 weeks. Cell viability of L929 cell line decreased specifically at the concentration of 2000 ${\mu}$g/mf of PLX. The cytotoxicity of PLX to the LNCaP cell line was shown at above 500 ${\mu}$g/ml of PLX. The inhibitory effect of PLX to the expression of PSA and 5${\alpha$}$-reductase genes in LNCaP cell line increased with the concentration of PLX. In vivo study, the results of PLX and finasteride administered group were 3.75${\pm}$0.60 and 3.49${\pm}$0.49 prostate weight ${\times}10^3$/body weight, which were lower than the result of BPH induced group (4.74${\pm}$0.58). These results suggested that PLX may be an effective material in BPH by having the role of the 5a-reductase inhibitor.

• 생명과학회지
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• 제32권9호
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• pp.712-720
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• 2022

비록 PM_2.5 노출과 다양한 안구 표면 질환과 관련성이 많은 선행 연구에서 알려졌지만, PM_2.5 가 각막에 미치는 세포 독성에 대한 연구는 거의 수행되지 않았다. 본 연구의 목적은 PM에 의한 각막 상피세포의 유해성을 평가하기 위한 in vitro 모델로서 쥐의 각막유래 상피세포(primary rat corneal epithelial cells, RCE cells)의 효능을 조사하는 것이다. 이를 위하여 쥐의 눈에서 분리한 1차 배양 세포가 각막 상피세포임을 pan-cytokeratin 염색을 통하여 확인하였으며, PM_2.처리에 의한 각막 상피세포의 형태학적 변화를 동반한 생존율의 억제는 세포사멸 유도와 관련이 있었다. 또한 PM_2.가 처리된 각막 상피세포에서는 ROS의 생성이 증가되었으며, 이는 미토콘드리아 기능 장애와 연관성이 있었다. 이와 함께 PM_2.는 각막 상피세포에서 NO, TNF-α, IL-1β 및 IL-6를 포함한 염증 매개인자 및 사이토카인의 생성을 증가시켰다. 아울러 heatmap 분석을 통해 BLNK, IL-1RA, Itga2b, ABCb1a 및 Ptgs2가 미세먼지 유도 안구 질환의 임상 치료를 위한 잠재적인 표적 유전자로서 제시하였다. 결론적으로 본 연구의 결과는 1차 쥐의 각막 상피세포가 PM_2.에 의한 각막 상피세포 병리기전 연구에 유용한 모델일 수 있으며, 산화적 및 염증성 반응이 PM_2.유발 안구 표면 장애 유도에 핵심적인 역할을 함을 알 수 있었다.

• Natural Product Sciences
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• 제28권2호
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• pp.62-67
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• 2022

Column chromatographic fractionation of the methanol and ethyl acetate extracts of the stem-bark of Anacardium occidentale led to the isolation of five compounds (1-5). Their structures were determined by spectroscopic means by comparing spectral data to be β-sitosterol (1), 2,4-dihydroxy acetophenone (2), 1-monolinolein (3), ethyl oleate (4) and β-sitosterol-3-O-β-D-glucopyranoside (5). These compounds were evaluated for cytotoxicity against human cancer cell lines: A549, SCOV3 and rat normal cell line NRK49f. Compounds 2-5 were for the first time isolated from A. occidentale.

• 생약학회지
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• 제34권2호통권133호
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• pp.132-137
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• 2003

The effect of aqueous extract of Isodon japonicus Hara (Labiatae) (IJAE) on mast cell-mediated immediate-type allergic reactions was investigated. IJAE inhibited compound 48/80-induced systemic anaphylaxis and immunoglobulin E (IgE)-mediated local anaphylaxis. When IJAE was pretreated at the same concentration with systemic anaphylaxis, serum histamine levels were reduced in a dose-dependent manner. IJAE dose-dependently inhibited histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80. The level of cAMP in human mast cell line (HMC-1) cells, when IJAE was added, significantly was increased, compared with that of normal control. These results indicate that IJAE will beneficial in the treatment of immediate-type allergic reaction.

• Advances in Traditional Medicine
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• 제10권3호
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• pp.200-207
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• 2010

The fruits of Piper cubeba L. are used traditionally to treat respiratory disorders in Indonesia. In order to determine the compounds responsible for this activity, the fruits were extracted with nhexane followed by ethanol to give n-hexane and ethanol extracts. Based on tracheospasmolytic assay on these two extracts, the n-hexane extract was more active to inhibit trachea contraction than that of ethanol extract. Upon bioassay guided isolation of the n-hexane extract, a tracheospasmolytic active compound was isolated and identified as dihydrocubebin [(3,4),(3',4')-bis-methylenedioxy-9,9'dihydroxylignan] $(\underline{1})$. Compound $\underline{1}$ was tested further for its ability to inhibit histamine released from mast cells, using rat basophilic leukemia (RBL-2H3) cell line and rat peritoneal mast cells RPMCs) as models; and $DNP_{24}$-BSA, thapsigargin, ionomycin, compound 48/80 and PMA were used as inducers for histamine released from mast cell. The test result showed that $\underline{1}$ inhibited histamine release from RBL-2H3 cells induced by $DNP_{24}$-BSA, thapsigargin and ionomycin. In addition, $\underline{1}$ suppressed histamine release from RPMC induced by either thapsigargin or ionomycin. However, $\underline{1}$ did not inhibit histamine release from RPMC induced by either compound 48/80 or combination PMA-sub optimum dose of ionomycin. Therefore, it was concluded that the inhibitory effects of $\underline{1}$ on the histamine released from mast cells may involve mechanisms related to intracellular $Ca^{2+}$ signaling events or downstream processes of intracellular $Ca^{2+}$ signaling in mast cells.

• 한국가축번식학회지
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• 제23권4호
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• pp.347-352
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• 1999

1. 유전자 재 조합 호르몬 (eCG IPMSG, hCG 및 hFSH)의 활성체크 및 세포내 signal transduction에 관한 기초연구를 위하여 rat의 융모성 성선자극 호르몬 수용체 (LH/CGR) 및 난포 자극호르몬 수용체 (FSHR)를 이미 보고되어진 염기배열에 의하여 PCR 방법으로 크로닝 하여, human embryonic kidney 유래의 293 세포에 transfection 하여 세포 표면에 LH/CGR와 FSHR를 발현하는 cell lines을 분리하였다. 2. hCG와 FSH의 signal을 전달하는 능력은 hCG와 FSH 또는 eCG의 농도증가에 따라 이들 수용체로 하여금 세포내의 cAMP 분비가 증가함을 알 수 있었다. 즉, transfection 되어진 이들 수용체를 발현하는 수용체의 대부분은 ligand binding 기능올 가지고, cAMP 반응에 의한 생리활성을 분석할 수 있으며, 또한 유전자 재조합당 단백질 호르몬 (eCG, hCG 및 hFSH)의 signal transduction, 구조 및 기능연구에 활용할 수 있다.