• Title/Summary/Keyword: Rapid Mixing

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Molecular identification of sweet potato accessions using ARMS-PCR based on SNPs

  • Park, Hyungjun;Kim, Sujung;Nie, Hualin;Kim, Jiseong;Lee, Jeongeun;Kim, Sunhyung
    • Journal of Plant Biotechnology
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    • v.47 no.2
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    • pp.124-130
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    • 2020
  • The sweet potato (Ipomoea batatas [L.] Lam.) is the sixth-most important crop in the world following rice, wheat, potato, maize, and cassava. Four varieties ('Beniharuka', 'Annobeni', 'Pungwonmi', 'Hogammi') and their Japanese cultivars are broadly distributed in South Korea. In the Korean marketplace, sweet potatoes are classified by color and shape, not by variety, making it necessary to differentiate varieties for uniform production and consumption. In this study, molecular markers were developed to distinguish the four varieties of sweet potato using SNPs and genotyping-by-sequencing (GBS) analysis via a tetra-primer amplification refractory mutation system (ARMS)-PCR. The results revealed that three variety-specific fragments (164 bp and 241 bp of SNP 04-27457768 and 292 bp of SNP 03-16195623) were amplified in the 'Beniharuka', 'Pungwonmi', and 'Annobeni' sweet potato varieties. There were instances where some varieties produced three bands within the gel electrophoresis, indicating heterozygosity at the given SNPs loci. DNA sequencing analysis also confirmed the results of electrophoresis at the SNPs loci. Overall, these molecular markers would provide a useful, rapid, and, simple evaluation method for the Korean sweet potato marketplace, where the mixing of varieties is a serious issue.

Quality Characteristics and Optimization of Ingredients of Sweet Rice Paste as a Replacement of Red Bean Paste (단팥앙금 대체제로서 쌀앙금의 품질특성 및 재료배합비 최적화)

  • Kim, Mi Jin;Lee, Bo Young;Lee, Young-Eun
    • Journal of the Korean Society of Food Culture
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    • v.32 no.1
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    • pp.58-65
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    • 2017
  • This study was conducted to optimize the conditions of a sweet rice paste as a replacement of red bean paste recipe using the central composite design of response surface methodology (RSM). Ten experimental recipes with two reference points (rice flour and sugar), were selected, and the physical and sensory characteristics of a sweet rice paste were measured. In the Rapid Visco Analyzer test, breakdown value was the highest in rice flour (105.73 RVU). Sweetness significantly increased with addition of sugar contents (p<0.0002) and viscosity increased with addition of rice flour contents (p<0.0185). Moisture contents decreased with increasing contents of rice flour and sugar, whereas yellowness and redness tend to increase. In the sensory evaluation test, quadratic models for color (p<0.01865), sweetness (p<0.0399), thickness (p<0.0073), and overall acceptability (p<0.0249) were approved as independent for the rice flour and sugar contents. In conclusion, the optimal mixing ratio of sweet rice paste was determined to be 45.38 g of rice flour and 77.95 g of sugar.

Mechanical Properties and Fabrication of Nanostructured ReSi1.75 by High Frequency Induction Heated Combustion Synthesis (고주파유도가열 연소합성에 의한 나노구조 ReSi1.75 제조 및 기계적 성질)

  • Kim, Byung-Ryang;Kim, Su-Chul;Shon, In-Jin
    • Journal of Powder Materials
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    • v.16 no.1
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    • pp.16-21
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    • 2009
  • Nanostructured $ReSi_{1.75}$ was synthesized to have high density via rapid and cost effective process named as high-frequency induction heated combustion synthesis(HFIHCS) method. For the process, mechanically activated Re-Si powder was used, which had been prepared by mechanical ball milling of Re and Si powders with mixing ratio of 1:1.75. Both combustion synthesis and densification were accomplished simultaneously by applying electric current and mechanical pressure of 80 MPa during the process. The average grain size, hardness, and fracture toughness of the compound were 210 nm, 1085 $kg/mm^2$ and 4 $MPa{\cdot}m^{1/2}$, respectively. The experimental results show that HFIHCS is a promising process for synthesis of nanostructured $ReSi_{1.75}$ which has a potential for both high temperature and thermo-electric applications.

Numerical Simulation Study on Supersonic Combustion using the Cavity (공동을 이용한 초음속 연소의 수치적 연구)

  • Jeong, Eun-Ju;Jeung, In-Seuck
    • 한국연소학회:학술대회논문집
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    • 2005.10a
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    • pp.255-260
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    • 2005
  • To achieve efficient combustion within a manageable length, a successful fuel injection scheme must provide rapid mixing between the fuel and airstreams. The aim of the present numerical research is to investigate the flame holding and combustion enhancement. Additional fuel into the cavity prevents shear flow impingement on the trailing edge of the cavity. The high temperature freestream flow mixes with the cold hydrogen fuel that is injected into the cavity and raises the fuel temperature remarkably and become to start combustion. The high pressure in the cavity due to the cavity structure and combustion leads the hydrogen fuel to upstream. The shock in the cavity to be generated by the fuel injection joins together and reflects off the ceiling wall. This makes high pressure and low mach number region and makes a small recirculation in this region. This high stagnation temperature is nearly recovered in the shear layer in front of the cavity and leads to start combustion. In the downstream of the cavity, the wall pressure drops significantly. This means that the combustion phenomenon is diminished. Because fuel lumps at the trailing edge of the cavity then it spreads after the cavity so, in this region there is a strong expansion.

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Texturing of YBa$_2Cu_3O_x$ thick film on MgO(001) single crystal (YBa$_2Cu_3O_x$ 후막의 단결정 MgO(001) 위에서의 배향화)

  • Kim, Eu-Gene;Kim, Myeong-Hui;Han, Young-Hee;Sung, Tae-Hyun;Kim, Sang-Joon;No, Kwang-Soo
    • 한국초전도학회:학술대회논문집
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    • v.9
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    • pp.271-274
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    • 1999
  • We are investigating epitaxially grown YBa$_2Cu_3O_x$(123) on MgO single crystal by partial melting process for high power application. After fabricating of BaCuO$_2$(011), Y$_2BaCuO_5$(211) powder, we made YBa$_2Cu_3O_x$(123) Paste with just mixing of (211), (011) and CuO(001) powders. Screen printing method was used to coat YBa$_2Cu_3O_x$(123) paste on MgO single crystal. To reduce the reaction in low temperature, rapid heating was conducted at partial melting temperature. The film was analysed with the difference of cooling-rate, thickness, reaction temperature by XRD, SEM, in-plane alignment, out-of-plane alignment, temperature-resistivity characteristics.

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The Remodelling of Hydraulic Structure in a Distribution Channel for Improving the Equality of the Flow Distribution (I): Design Using CFD Simulation (수리구조 개선을 통한 분배수로 균등분배 성능 향상에 관한 연구(I) : CFD를 이용한 설계 중심으로)

  • Park, No-Suk;Kim, Seong-Su;Park, Jong-Yoon;Yoon, Cheol-Hwan;Kim, Chung-Hwan
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.5
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    • pp.571-579
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    • 2007
  • This study was conducted to qualify the equality of the flow distribution from open channel between rapid mixing basin and flocculation basins in a domestic full-scale water treatment plant, and suggest a remedy for improving the equality. In order to evaluate the feasibility of the suggested remedy, computational fluid dynamics (CFD) technique are used, and for verifying the CFD simulation results wet tests were carried out for the pilot scale channel based on geometric similarity. From the results of CFD simulation and wet tests, it was investigated that the modification of hydraulic structure in the distribution channel, which is to install the longitudinal orifice baffle in flow direction, could improve the equality of the flow distribution. Also, in the case that Froude number is relatively small (Froude number <<0.03), the open ratio of orifices on the installed baffle hardly affects the equality of flow distribution.

Characteristics and Microstructure of Co/Ni Composite Silicides on Polysilicon Substrates with Annealing Temperature (폴리실리콘 기판 위에 형성된 코발트 니켈 복합실리사이드 박막의 열처리 온도에 따른 물성과 미세구조변화)

  • Kim, Sang-Yeob;Song, Oh-Sung
    • Korean Journal of Materials Research
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    • v.16 no.9
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    • pp.564-570
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    • 2006
  • Silicides have been required to be below 40 nm-thick and to have low contact resistance without agglomeration at high silicidation temperature. We fabricated composite silicide layers on the wafers from Ni(20 nm)/Co(20 nm)/poly-Si(70 nm) structure by rapid thermal annealing of $700{\sim}1100^{\circ}C$ for 40 seconds. The sheet resistance, surface composition, cross-sectional microstructure, and surface roughness were investigated by a four point probe, a X-ray diffractometer, an Auger electron spectroscopy, a field emission scanning electron microscope, and a scanning probe microscope, respectively. The sheet resistance increased abruptly while thickness decreased as silicidation temperature increased. We propose that the fast metal diffusion along the silicon grain boundary lead to the poly silicon mixing and inversion. Our results imply that we may consider the serious thermal instability in designing and process for the sub-0.1 um CMOS devices.

Heat Treatment Effect on the Microstructure of 8YSZ Thick Film (열처리 온도에 따른 8YSZ 후막의 미세구조)

  • Han, Sang-Hoon;Noh, Hyo-Seop;Na, Dong-Myung;Jin, Guang-Hu;Lee, Woon-Young;Park, Jin-Seong
    • Journal of the Korean Ceramic Society
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    • v.48 no.1
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    • pp.106-109
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    • 2011
  • In order to fabricate 8YSZ thick film by silk screen printing, YSZ(yttria-stabilized zirconia) commercial powder was used as starting materials. Paste for screen printing was made by mixing 8YSZ powder and organic vehicles. 8YSZ thick film was formed on $Al_2O_3$ substrate. The crystal structure, and microstructure were investigated. Grain size of 8YSZ was increased with increasing calcination temperature and rapid grain growth was shown after calcination at $1300^{\circ}C$. Microstructure showed the mixture of large and small grain size after $1400^{\circ}C$ sintering. Shrinkage rate of 8YSZ thick film sintered at $1400^{\circ}C$ was more than 40%.

Optimal Design of Orifice typed Distribution Channel using Step Method Program (Step method 프로그램을 이용한 orifice 분배수로의 최적 설계에 관한 연구)

  • Park, No-Suk;Park, Sangcheol;Kim, Sung-soo;Lee, Seonjoo;Jeong, Nam-Jeong
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.5
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    • pp.691-700
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    • 2006
  • This study conducted to optimize the design and operation of orifice typed distribution channels which were generally constructed to link the rapid mixing process and flocculation/sedimentation basin. To accomplish the goal of this study, programming step method using FORTRAN 90, was applied it to simulate the performance of existing distribution channel in the selected S DWTP (Drinking Water Treatment Plant). The proposed step method program was validated in terms of the feasibility with comparison between the measurement and prediction value in each orifice. From the evaluation results of the current conditions with the design and operation, it was revealed that the existing gradient of the tapered channel is not appropriate. Also, we suggested that in the case of the inlet width being 3.5m, reducing the downstream width by about 0.5m would make more equitable distribution flow in the channel. Consequently, dealing with various conditions of the design and operation with distribution channel, we could conclude that for the parallel typed channel, as the width is wider and the diameter of orifice is smaller, the more equitable distribution occur. In addition, the inlet flowrate and the number of orifice can affect the flow velocity in the channel.

Effect of Heating Rate on the Behavior of the Flame Front in the Pulverized-Coal Flame (미분탄화염에서 가열률이 화염선단의 거동에 미치는 영향)

  • Cho, Han Chang;Park, Jung Kyu;Shin, Hyun Dong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.23 no.5
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    • pp.687-694
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    • 1999
  • An experimental study was carried out in two laboratory-scale reactors to investigate the effect of heating rate on the behavior of flame front in a pulverized coal flame. Each. reactors had different heating mechanisms. For reactor A losing large heat through transparent quartz wall. pulverized coal particles were ignited by secondary air of 1050K. Flame front could be visualized through the transparent wall. Reactor B was insulated with castable refractory to minimize the heat loss through the reactor wall and accompanied with secondary air of 573K. Flame front was estimated from the gas temperature and species concentration measured using R-type thermocouple(Pt-Pt/Rh 13%) and gas chromatograph at various coal-air ratios and swirl intensities. The flame front position was closely related with the magnitude of heating rate. The heating rate for lifted flame was of the order of $10^4$ to $10^5K/s$ and for coal Ignition at least over $10^4K/s$. The heating mechanism had little impact on the extinction limits. The weak swirl number of 0.68 forced the flame front to move toward the upstream by the rapid mixing of coal and air. The primary/secondary momentum ratio was an inappropriate variable to distinct the liftoff of flame.