• 제목/요약/키워드: Random amplified polymorphic DNA

검색결과 342건 처리시간 0.031초

Mycoflora of Chicken-Viscera with Aid of RAPD Technique as a Tool for Confirmation

  • Gherbawy, Youssuf A.;Farghaly, Refaat M.
    • Mycobiology
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    • 제30권1호
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    • pp.5-12
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    • 2002
  • A total of 100 samples of chicken-viscera were collected from different poultry-slaughtering houses in Austria;(20 samples of each of gizzard, heart, intestine, liver and spleen). Intestine and gizzard were heavy contaminated with moulds than other examined visceral organs($4.4{\times}10^5$ and $2.6{\times}10^4$ colonies/1g of the samples, respectively). Fungal contamination was not detected in all samples of heart and spleen. Eighty-five mould isolates were collected from the examined samples, the majority of isolates belonging to Aspergillus glaucus group(20.0%) and Trichoderma(14.1%). These isolates comprised 15 species belonging to 9 genera. Members of Aspergillus glaucus(telomorph: Eurotium) group and Trichoderma were further confirmed their identification using random amplified polymorphic DNA-polymerase chain reaction(RAPD-PCR) technique.

닭 도체에서 분리한 Salmonella spp의 특성 분석 (Characteristics of Salmonella spp isolated from poultry carcasses)

  • 이호원;홍종해;정병열
    • 한국동물위생학회지
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    • 제30권3호
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    • pp.339-351
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    • 2007
  • Salmonella infections cause the diseases in poultry and some zoonotic Salmonella can be transmitted to human through poultry products, resulting in food-borne disease. This study was conducted to obtain some useful information for the control of salmonellosis in human. Twenty four Salmonella spp were isolated from poultry carcasses, and they were examined with several methods such as serotyping, antimicrobial resistance test and random amplified polymorphic DNA(RAPD) to identify their characteristics. In serotyping test of 24 strains S enteritidis was 17 (70.8%), followed by S schwarzengrund 3 (12.5%), untyped strain 4 (16.7%). In the results of antimicrobial resistance test, 23 (95.8%) isolates were resistant to at least one antimicrobial agent, generating eight different resistance patterns. In RAPD analysis using URP-6 primer to differentiate Salmonella isolates within a serotype, 4 serogroups were divided into 10 RAPD types: 5 types in S enteritidis, 2 types in S schwarzengrund and 3 types in the remainder.

RAPD 분석과 내부형태에 의한 독활(獨活)(Aralia continentalis)의 감별에 관한 연구 (Discrimination of Aralia continentalis Root by the Random Amplified Polymorphic DNA Analysis and Morphological Characteristics)

  • 이미영;주영승;김홍준;고병섭
    • 한국한의학연구원논문집
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    • 제7권1호
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    • pp.145-152
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    • 2001
  • Dried parts of the herb medicines are difficult to distinguish morphologically. Heracleum moellendorffii cordata has often been sold instead of Aralia cordata in herbal medicine markets. Therefore, this study was conducted to develop the key for discrimination between them using the RAPD analysis and morphological characteristics. Thirty decarmer oligonucleotide primers were screened for the RAPD analysis, and four primers generated distinct RAPD markers specific to Aralia cordata, Angelica pubescens maxim f. biserrata, and Heracleum moellendorffii. The specific RAPD patterns generated by the selected primers were reproducible from dried materials. In comparison of morphological characteristics, Aralia cordata seems to be entirely developed in xylem fiber, but not developed in pith.

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큰느타리버섯의 저온적응성 형질에 관련된 SCAR Marker 개발 (Development of a psychrophilic-SCAR marker for Pleurotus eryngii)

  • 김수철;황혜성;조윤진;김혜수;류재산;조수정
    • 한국버섯학회지
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    • 제11권3호
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    • pp.171-176
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    • 2013
  • 큰느타리버섯의 저온성적응성 형질에 관련된 SCAR marker를 개발하기 위하여 저온성 계통의 8균주와 대조구 8균주의 genomic DNA를 30 ug/ml의 농도로 bulking한 것을 주형 DNA로 사용하고 operon 사의 OPA(20개), OPB(20개), OPL(20개), OPP(20개), OPR(20개), OPS(20개) 등 총 120개 primer를 random primer(10 mer)로 사용하여 RAPD를 수행하였으며 이중에서 OP-S3 primer를 사용한 PCR 산물들이 대조구와 가장 뚜렷한 차이를 나타내었다. OP-S3 primer를 이용한 RAPD 결과, 약 480 bp 부근에서 저온성 계통에 특이적인 DNA band가 관찰되었으며 이 DNA band의 염기서열을 근거로 SCAR marker로 사용할 specific primer인 OP-S3-1-F와 OP-S3-1-R를 디자인하였다. SCAR marker OP-S3-1 primer를 이용하여 PCR을 수행한 결과에서는 저온성 계통에서만 480 bp 부근에서 대조구와 구별되는 DNA band를 확인할 수 있었으며 random primer인 OP-S3 primer를 이용하여 PCR을 수행했을 때보다 재현성이 높고 진한 DNA band를 확인할 수 있었다.

민자주방망이버섯(Lepista nuda)의 균사배양 및 유전적 특성에 관한 연구 (A STUDY ON THE MYCELIA CUL TURE AND GENETIC CHARACTERISTICS IN Lepista nuda)

  • 김종봉;황성구
    • 생명과학회지
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    • 제11권5호
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    • pp.496-501
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    • 2001
  • 민자주방망이버섯 균사의 배양특성을 알아보기 위하여 PDA, YM, MEA, GPB, Yamanaka의 5종 배지를 사용하여 배양한 결과, 균근성버섯의 적정배지로 보고된 바 있는 Yamanaka 배지에서 가장 양호한 성장을 보여 주었다. pH 별 균사생장속도를 알아보기 위하여 pH 4, 5, 6, 7, 8의 조건으로 배양실험을 실히한 결과 pH 6이 가장 양호하였으며, 적정탄소원에 대한 실험에서는 glucose, sucrose, starch 중 starch를 탄소원으로 첨가한 배지에서 가장 왕성한 성장을 보여 주었다. 적정질소원에 대한 실험에서는 ammo-nium tartrate, yeast extract, peptone, asparagine 중 yeast extract와 peptone을 첨가한 배지에서 성장이 활발하므로 유기태질소원의 이용이 용이한것으로 보인다. 유전적특성을 조사하기 위하여 RAPD분석을 시행한 결과, 느타리버섯속과 자주방망이버섯속의 비교에서 primer에 따른 밴드패턴의 상이한 점을 발견하였다.

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Variations in Sweetpotato Regenerates from Gamma-ray Irradiated Embryogenic Callus

  • Lee, Young-Ill;Lee, In-Sok;Lim, Yong-Pyo
    • Journal of Plant Biotechnology
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    • 제4권4호
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    • pp.163-170
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    • 2002
  • Radiation induced and somaclonal variations were investigated in the regenerates from gamma irradiated and controlled embryogenic callus (EC) of sweetpotato cvs., Yulmi and White Star by morphological, RAPD and AFLP analysis. Most (approx. 90%) of the EC produced somatic embryos developed into plantlets after being transferred to the auxin-free medium. The frequency of morphological variants derived from the irradiated callus ranged from 3 to 7.8% compared to 0.1-1.1% of that derived from the non-irradiated. Morphological variants were selected from the regenerates and analyzed by RAPD and AFLP procedures. RAPD polymorphisms of Yulmi and White Star regenerates from irradiated calli were 8.8% and 6.1%, respectively. However, the polymerphisms among regenerates from the non-irradiation treatment in these two cultivars were non-detectable and 3%, respectively. AFLP polymorphisms of Yulmi and White Star regenerates from irradiated calli were 29.9% and 28.6%, respectively. while the frequencies for those form non-irradiated calli were 8.5% and 5.6%, respectively. Both the control plants and variants from the nonirradiated were clustered together, while variants from irradiated were separated from the group by Nearest-Neighbor-Interchange Branch Swapping Abbreviation: EC (Embryogenic callus), AFLP (Amplified Fragment Length Polymorphism), RAPD (Random amplified polymorphic DNA)

RAPD 방법을 이용한 반하류 한약재의 감별 연구 (RAPD Analysis on the Species of Pinelliae Tuber)

  • 배명효;김규열;정유헌;최호영
    • 대한한의학회지
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    • 제20권4호
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    • pp.16-22
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    • 2000
  • This study intends to report the significance of several experimental results obtained from analysing the genes extracted from the plants and herbal medicine such as P. temalta (Thunb.) Breit, A. amurense var serratum Nakai, A. erubescens (Wall.) Schott, Pinelliae Tuber and Arisaematis Tuber, mainly by the method of RAPD(randomly amplified polymorphic DNA) and the method of RFLP(restriction fragment length polymorphism) on ITS(internal transcribed spacer) region. Genomic DNA could be extracted from both original plants and dried materials. DNA fragments of P. temata kind and A. amurense kind showed the same aspect separately within the same species under the method of RAPD using random primer, while various aspects(polymorphism) were discovered among different species. In RAPD analysis by uniprimer, common bands were extracted from all types of P. temata in the case of uniprimer #4, which were distinguished from the kind of A. amurense. Other polymorphic bands appeared in between different A. amurense species as well. In the case of uniprimer #11, particular band came out in the kind of P. temata. On the other hand, in the case of uniprimer #5, #6, and #8, various bands(polymorhism) were revealed in both kinds of P. temata and A. amurense. Although further study is needed to ascertain whether these results are due to the differences of species, kinds, or growing place, the results could be used as a scientific method of identifying the substitutes for A. amurense genus. The author believes that as if P. temata class of plants used in this experiment are different among themselves in terms of the shape, size and property, those are clearly a class of P. temata or belong to the same genus.

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사상의학의 객관화를 위한 유전적 분석 연구 (Genetic analysis study of Sasang Constitution classification)

  • 조동욱;이창수;고병희;홍석철;이의주;권건혁;조황성
    • 한국한의학연구원논문집
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    • 제2권1호
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    • pp.402-406
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    • 1996
  • The main idea of Sasang medicine is that everyone has his own unique constitution. There are four kinds of constitutions and these are Taeyang, Soyang, Teaum and Soum. Although Sasang medicine is a unique and originative medical theory with creative ideas of Korean traditional medicine, the classification of individual constitution mainly depends on the methods which may not be objectively proven and scientifically understood. This study was carried out tn establish scientific understanding of Sasang medicine by investigating the possible genetic difference among four constitutions. Sasang constitution classification was performed by Sasang medicine research group in Korea Institute of Oriental Medicine and Kyung Hee University, Genomic DNA was prepared from the blood of individuals of different constitutions and RAPD(Random amplified polymorphic DNAs) analysis was then carried out to investigate the possible difference among different constitutions on DNA level. For RAPD analysis, ten different random primers were applied to Teaum, Soyang and Soum group DNA samples and the presence of DNA markers specific for each constitution was investigated.

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Genetic characterization of microsporidians infecting Indian non-mulberry silkworms (Antheraea assamensis and Samia cynthia ricini) by using PCR based ISSR and RAPD markers assay

  • Hassan, Wazid;Nath, B. Surendra
    • International Journal of Industrial Entomology and Biomaterials
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    • 제30권1호
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    • pp.6-16
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    • 2015
  • This study established the genetic characterisation of 10 microsporidian isolates infecting non-mulberry silkworms (Antheraea assamensis and Samia cynthia ricini) collected from biogeographical forest locations in the State of Assam, India, using PCR-based markers assays: inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD). A Nosema type species (NIK-1s_mys) was used as control for comparison. The shape of mature microsporidian spores were observed oval to elongated, measuring 3.80 to $4.90{\mu}m$ in length and 2.60 to $3.05{\mu}m$ in width. Fourteen ISSR primers generated reproducible profiles and yielded 178 fragments, of which 175 were polymorphic (98%), while 16 RAPD primers generated reproducible profiles with 198 amplified fragments displaying 95% of polymorphism. Estimation of genetic distance coefficients based on dice coefficients method and clustering with un-weighted pair group method using arithmetic average (UPGMA) analysis was done to unravel the genetic diversity of microsporidians infecting Indian muga and eri silkworm. The similarity coefficients varied from 0.385 to 0.941 in ISSR and 0.083 to 0.938 in RAPD data. UPGMA analysis generated dendrograms with two microsporidian groups, which appear to be different from each other. Based on Euclidean distance matrix method, 2-dimensional distribution also revealed considerable variability among different identified microsporidians. Clustering of these microsporidian isolates was in accordance with their host and biogeographic origin. Both techniques represent a useful and efficient tool for taxonomical grouping as well as for phylogenetic classification of different microsporidians in general and genotyping of these pathogens in particular.

베타글루칸 함량이 높은 큰느타리버섯 선발을 위한 SCAR marker 개발 (Development of strain-specific SCAR marker for selection of Pleurotus eryngii strains with higher β-glucan)

  • 김수철;김혜수;조용운;류재산;조수정
    • 한국버섯학회지
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    • 제13권1호
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    • pp.79-83
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    • 2015
  • 본 연구는 큰느타리버섯의 베타글루칸 고함유 형질에 관련된 SCAR marker를 개발하기 위해 수행되었다. operon사의 OPA(20개), OPB(20개), OPL(20개), OPP(20개), OPR(20개), OPS(20개) 등 총 120개 primer를 random primer(10 mer)로 사용하여 대립 계통 9종과 베타글루칸 고함유 계통 9 종을 대상으로 RAPD를 이용한 bulked segregant analysis를 실시하여 OP-R03 primer로부터 대립 계통에는 나타나지 않고 베타글루칸 고함유 계통에만 나타나는 특이적인 RAPD 밴드를 얻었다. OP-R03 primer를 이용한 RAPD 결과, 약 91 bp 부근에서 베타글루칸 고함유 계통에 특이적인 DNA 밴드가 관찰되었으며 이 DNA 밴드의 염기서열 말단을 근거로 SCAR 마커로 사용할 specific primer인 OP-R03-1-F와 OP-R03-1-R를 디자인하였다. SCAR 마커 OP-R03-1-F/-1-R primer를 이용하여 PCR을 수행한 결과에서도 91 bp 부근에서 대립 계통과 구별되는 DNA 밴드가 베타글루칸 고함유 계통에서 확인되었으며 random primer인 OP-R03 primer를 이용하여 PCR을 수행했을 때보다 재현성이 높고 진한 DNA 밴드임을 확인할 수 있었다.