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http://dx.doi.org/10.4489/MYCO.2002.30.1.005

Mycoflora of Chicken-Viscera with Aid of RAPD Technique as a Tool for Confirmation  

Gherbawy, Youssuf A. (Botany Dept., Faculty of Science, South Valley University)
Farghaly, Refaat M. (Food Hygiene Dept., Faculty of Veternary Medecine, South Valley University)
Publication Information
Mycobiology / v.30, no.1, 2002 , pp. 5-12 More about this Journal
Abstract
A total of 100 samples of chicken-viscera were collected from different poultry-slaughtering houses in Austria;(20 samples of each of gizzard, heart, intestine, liver and spleen). Intestine and gizzard were heavy contaminated with moulds than other examined visceral organs($4.4{\times}10^5$ and $2.6{\times}10^4$ colonies/1g of the samples, respectively). Fungal contamination was not detected in all samples of heart and spleen. Eighty-five mould isolates were collected from the examined samples, the majority of isolates belonging to Aspergillus glaucus group(20.0%) and Trichoderma(14.1%). These isolates comprised 15 species belonging to 9 genera. Members of Aspergillus glaucus(telomorph: Eurotium) group and Trichoderma were further confirmed their identification using random amplified polymorphic DNA-polymerase chain reaction(RAPD-PCR) technique.
Keywords
Chicken; Viscera; Mould; RAPD-PCR;
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