• Title/Summary/Keyword: Rag

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Electrolyser for CECE Process

  • Lee, Min-Soo;Chung, Hong-Suk;Paek, Seung-Woo;Kim, Kwang-Rag;Yim, Sung-Paal;Ahn, Do-Hee
    • Proceedings of the Korean Nuclear Society Conference
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    • 2005.05a
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    • pp.197-198
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    • 2005
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Occurrence of a Natural Interspecific Hybrid between Rhodeus pseudosericeus and R. notatus in Sangcheon Stream of the Han River, Korea (한강수계 상천천에서 한강납줄개 Rhodeus pseudosericeus와 떡납줄갱이 R. notatus의 종간 자연잡종 출현)

  • Kwak, Yeong-Ho;Kim, Keun-Yong;Kim, Keun-Sik;Song, Ha-Yun
    • Korean Journal of Ecology and Environment
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    • v.53 no.3
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    • pp.275-285
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    • 2020
  • Two specimens presumed to be hybrids of Rhodeus pseudosericeus and R. notatus were collected from the Sangcheon Stream of the Han River, Korea. The body color of natural hybrid individuals was yellowish brown, showing the intermediate characteristics of R. pseudosericeus and R. notatus, but overall, the characteristics of R. notatus were similar. Meristic and morphometric characters, the number of dorsal fin rays, anal fin rays and longitudinal row scales showed a hybrid index (HI) of 0, indicating the characters of R. notatus. The hybrid index of predorsal length (HI=74.6), preanal length (HI=75.3), and preventral length (HI=77.6) were similar to the characters of the R. pseudosericeus. Also four characters were appeared to have intermediate characters between R. pseudosericeus and R. notatus; number of gill rakers (HI=55.3), body depth (HI=67.9), snout length (HI=43.4), and inter orbital width (HI=44.8). The rest of 14 characters deviated between 0 and 100, showing unique characteristics of hybrid individuals. Recombination activating gene 1 (RAG1) analysis, hybrids were identified as natural hybrids due to the overlapping peaks of their parent species. Also, as a result of analysis using the cytochrome b gene (COB), one individual was derived from R. pseudosericeus, while the other was derived from R. notatus.

Genetic Species Identification by Sequencing Analysis of Nuclear and Mitochondrial Genes for Albino Misgurnus Species from Korea (우리나라 미꾸리속(genus Misgurnus) 알비노 개체의 미토콘드리아 및 핵 유전자 염기서열 분석에 의한 유전적 동정)

  • Song, Ha-Youn;Moon, Shin-Joo;Kim, Keun-Sik;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.29 no.2
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    • pp.139-145
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    • 2017
  • The spontaneous color mutant, albino individuals of genus Misgurnus, are rarely discovered in Korea and there are difficult to identify morphological species due to lack melanin pigmentation. In this study, we developed a genetic identification method for the species of albino Misgurnus individuals based on phylogenetic analysis by using recombination activating gene 1 (rag1) and cytochrome b (cytb) region of mitochondrial DNA. As a result of molecular phylogenetic analysis, three clades were identified as Misgurnus mizolepis, M. anguillicaudatus and M. mohoity. The homology of the cytb sequences of M. mohoity was best match to that of M. mohoity sequences in GenBank database. As a result of species identification of 25 albino Misgurnus individuals based on the phylogenetic tree, the red-eye type was identified as 16 M. anguillicaudatus and one M. mizolepis. The remaining three individuals were identified as one M. mizolepis ♀${\times}$M. anguillicaudatus ♂, and two M. mohoity ♀${\times}$M. anguillicaudatus ♂, respectively. In addition, the five black-eye type individuals were identified as one M. anguillicaudatus, three M. mizolepis and one M. mohoity. Therefore, this genetic identification method will be an useful techniques for species or hybrid identification in genus Misgurnus.

Occurrence of a Natural Intergeneric Hybrid between a Female Tanakia lanceolata and a Male Rhodeus pseudosericeus (Cypriniformes: Cyprinidae) in Daecheoncheon Stream Flowing into the Yellow Sea in the Republic of Korea (서해안 독립 하천 대천천에서 납자루 Tanakia lanceolata (♀)와 한강납줄개 Rhodeus pseudosericeus(♂)의 자연 속간잡종 출현)

  • Kim, Yong Hwi;Sung, Mu Sung;Yun, Bong Han;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.33 no.2
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    • pp.45-56
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    • 2021
  • A male, presumed to be an intergeneric hybrid between Tanakia lanceolata and Rhodeus pseudosericeus, was collected in the Boryeong Daecheoncheon Stream flowing into the Yellow Sea in the Republic of Korea. Morphological and molecular phylogenetic analyses were performed to discriminate the definite origin of the estimated natural hybrid. As a result of the morphological analysis, the color of the dorsal and anal fin rays edges of the natural hybrid individual, the upper and lower body colors followed the morphological characteristics of T. lanceolata, and that blue longitudinal stripe in the center of the caudal peduncle, the incomplete lateral line, and the barbels absent followed the morphological characteristics of R. pseudosericeus. In addition, as a result of the cytochrome b (cytb) gene analysis of mitochondrial DNA (mtDNA), the natural hybrid showed a nucleotide sequence similarity of 99.82 to 100% with T. lanceolata, and the maternal species was identified as T. lanceolata. As a result of the recombination activating gene 1 (rag1) gene analysis of nuclear DNA (nDNA), the natural hybrid showed double peaks pattern reflecting both the single nucleotide polymorphism sites (38 bp) between T. lanceolata and R. pseudosericeus, and the paternal species was identified as R. pseudosericeus. Therefore, a natural hybrid estimated male of Acheilognathinae analyzed in this study was found to be an intergeneric hybrid between a female T. lanceolata and a male R. pseudosericeus.

In vitro Digestibility and Sensory Properties of different Bap(Cooked Rice) (밥의 종류에 따른 in vitro 분해율 및 관능적 특성)

  • Kim, Yoon-Sun;Lee, Gui-Chu
    • Journal of the Korean Society of Food Culture
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    • v.22 no.6
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    • pp.820-826
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    • 2007
  • Different types of bap(cooked rice) was cooked using barley or/and SoRiTae with rice as the base. Total(TS), rapidly digestible(RDS), slowly digestible(SDS) and resistant(RS) starch fractions were determined. Other physicochemical properties such as moisture, protein, amylose contents, protein digestion in vitro and color values as well as sensory properties of different bap were also investigated. Cooked rice with SoRiTae(RiSo) showed the highest moisture content of 63.9%, whereas other bap showed simillar content ranging from 62.3-63.0%. Crude protein content of RiSo was the highest, while that of cooked rice(Ri) was the lowest(p<0.05). Amylose content of RiBa was the highest, while that of RiSo was the lowest(p<0.05). In in vitro protein digestibility(IVPD), cooked rice with barley and SoRiTae(RiBaSo) was the highest, while Ri was the lowest, showing no significant difference at p<0.05. In starch fractions, as barley or/and SoRiTae were added to rice, a decrease in RDS content and increases in SDS and RS contents were observed. In addition, starch digestion index(SDI), which derived as an indicator of their in vitro starch digestibility and rapidly available glucose(RAG) value, which determined as a predictor of potential glycemic response decreased. A decrease in L value from RiSo and RiBaSo, which comprised of SoRiTae and increases in a and b values in RiSo and RiBa were observed, respectively. All sensory parameters involving color, glossiness, sweet taste, wetness, roughness, hardness and stickiness were shown to be a significant difference except sweet taste among different bap(p<0.05). L value of instrumental characteristic was negatively correlated with color of sensory characteristic and a value was positively correlated. Significant negative correlation was found between RS content and glossiness, however, positive correlation with roughness and hardness, respectively. These results suggested that cooked rice mixed with barley and SoRiTae contain significant RS and SDS contents and may improve diabetes and hyperlipidemia, due to the lowering RDS and RAG, respectively.