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Genetic Species Identification by Sequencing Analysis of Nuclear and Mitochondrial Genes for Albino Misgurnus Species from Korea  

Song, Ha-Youn (Inland Fisheries Research Institute, National Institute of Fisheries Science)
Moon, Shin-Joo (Life Science and Biotechnology, Soonchunhyang University)
Kim, Keun-Sik (East Sea Research Institute, Korea Institute of Ocean Science and Technology)
Bang, In-Chul (Life Science and Biotechnology, Soonchunhyang University)
Publication Information
Korean Journal of Ichthyology / v.29, no.2, 2017 , pp. 139-145 More about this Journal
Abstract
The spontaneous color mutant, albino individuals of genus Misgurnus, are rarely discovered in Korea and there are difficult to identify morphological species due to lack melanin pigmentation. In this study, we developed a genetic identification method for the species of albino Misgurnus individuals based on phylogenetic analysis by using recombination activating gene 1 (rag1) and cytochrome b (cytb) region of mitochondrial DNA. As a result of molecular phylogenetic analysis, three clades were identified as Misgurnus mizolepis, M. anguillicaudatus and M. mohoity. The homology of the cytb sequences of M. mohoity was best match to that of M. mohoity sequences in GenBank database. As a result of species identification of 25 albino Misgurnus individuals based on the phylogenetic tree, the red-eye type was identified as 16 M. anguillicaudatus and one M. mizolepis. The remaining three individuals were identified as one M. mizolepis ♀${\times}$M. anguillicaudatus ♂, and two M. mohoity ♀${\times}$M. anguillicaudatus ♂, respectively. In addition, the five black-eye type individuals were identified as one M. anguillicaudatus, three M. mizolepis and one M. mohoity. Therefore, this genetic identification method will be an useful techniques for species or hybrid identification in genus Misgurnus.
Keywords
Loach; albino; Misgurnus; hybrid; molecular markers;
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