• Korean Journal of Pharmacognosy
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• v.20 no.4
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• pp.227-232
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• 1989

As a part of studies on the quality control of crude drug preparation (So-Shi-Ho-Tang), index components of Glycyrrhizae Radix were identified by TLC and quantified by HPLC. Specific red spot (Rf=0.47) was identified in acid hydrolysate of glycosidic fraction on silica gel plate with benzene/ethyl acetate (1 : 1, v/v). The content of glycyrrhizin was determined by quantification of glycyrrhetinic acid by HPLC on ${\mu}-Bondapak\;C_{18}$ column with $MeOH/H_2O/HAc$ (78 : 19 : 3, v/v). Its recovery rate in the extract granules, compared to the content in the Glycyrrhizae Radix, was $83.3{\pm}0.7%$.

• Korean Journal of Pharmacognosy
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• v.21 no.2
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• pp.158-162
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• 1990

As a part of studies on the quality control of crude drug preparation, So-Shi-Ho-Tang, index components of Scutellariae Radix were identified by TLC and quantified by HPLC. Wogonin and baicalein were identified in ethyl ether fraction on silica gel plate with benzene/ethyl acetate (1 : 1, v/v). The content of baicalin was determined by HPLC on ${\mu}-Bondapak\;C_18$ column with 0.5%-phosphoric acid/acetonitrile (73 : 27, v/v). Its recovery rate in the extract granules, compared to the content in the Scutellariae Radix, was $52.1\;{\pm}\;0.7%$.

• Natural Product Sciences
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• v.23 no.2
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• pp.132-138
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• 2017

This study was designed to investigate the synergetic hepatoprotective effects from a mixture of Korean Red Ginseng and Pueraria Radix on carbon tetrachloride ($CCl_4$)-induced hepatotoxicity in mice. Liver toxicity was induced by intraperitoneal administration of $CCl_4$ (0.6 mg/kg) in 12 groups of ICR mice. The negative control group was given $CCl_4$ without test samples and the normal group was given no treatment. Among treatment groups, the RGAP treatment (Korean Red ginseng acetic acid extract : Pueraria radix water extract, w/w, 38.4:57.6) decreased $CCl_4$-elevated ALT (101.60 IU/L), AST (833.89 IU/L), and LDH (365.02 IU/L) levels in the serum, and increased the SOD (11.03 unit/mg protein) and CAT (0.37 unit/mg protein) levels and the LPO levels ($59.09{\mu}M/g$ tissue) more than that in the mice group with $CCl_4$-induced control group hepatotoxicity. These results suggest that administration of a mixture of Korean Red ginseng and Pueraria radix decreases $CCl_4$-induced liver damage and enhances antioxidant activity in mice and imply that administration of the mixture in a certain ratio is more effective than single administration of either Korean Red ginseng or Pueraria radix alone.

• Journal of Pharmacopuncture
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• v.3 no.2
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• pp.79-97
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• 2000

Objectives : To investigate the effects of herb-acupunctures of Qi tonification herbs or formula on the rat immune depression induced by an anticancer drug, methotrexate (MTX) Methods: Animals were divided into 5 groups; Normal control group was not given any treatment. Immune depression was induced by oral administration of 1mg/kg MTX b.i.d for 4 days in Control, Sample I, II, and III groups. We treated on CV 4 (Guanyuan) with saline, Bojoongiggi-tang (Buzhongyiqi-tang), Ginseng Radix and Astragali Radix herb-acupunctures in Control, Sample I, II, and Ill groups, respectively. In both blood and spleen, the assessment of CD4+ T-cell count, CD8+ T-cell count, CD4/CD8 T-cell ratio was performed. Results: Here we show that 3 herb- acupuncture groups have an influence, to some extent, on CD4+ and CD8+ T-cell counts and CD4/CD8 T-cell ratio in both blood and spleen. Astragali Radix herb-acupuncture, in particular, was found to have significantly increased CD4+ T-cell count in blood and CD4/CD8 T-cell ratio in blood and spleen. Conclusions: The results of this study suggest that herb-acupunctures of Bojoongiggi-tang (Buzhongyiqi-tang), Ginseng Radix and Astragali Radix may have an influence over rat immune depression induced by MTX.

• Journal of Pharmacopuncture
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• v.3 no.2
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• pp.131-152
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• 2000

The effects of Cervus elaphus aquapuncuture and Ginseng Radix aquapuncuture on the growth, protein efficiency ratio, contents of total protein, total cholesterol, and triglyceride in serum were studied for thirty four days. The results were summarized as follows. 1. Weight significantly increased in $0.3\%$ Cervus elaphus aquapuncture group on Chunchu loci and in $0.2\%$ Ginseng Radix aquapuncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. 2. Protein efficiency ratio significantly increased in $0.2\%$ and $0.3\%$ of Cervus elaphus aquapuncture group on Chunchu loci and $0.1\%$, $0.2\%$ and $0.3\%$ of Ginseng Radix aquapuncture group on Chunchu loci compared to nonnal saline aquapuncture on Chunchu loci. 3. Total protein significantly increased in $0.2\%$ and $0.3\%$ of Cervus elaphus aquapuncture group on Chunchu loci compared to nonnal saline aqriapuncture On Chunchu loci. whereas it had a tendency to increase in $0.2\%$ Ginseng Radix aquapuncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. 4. Total cholesterol increased a little in $0.3\%$ Cervus elaphus aquapuncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. 5. Triglyceride significantly increased in $0.2\%$ Ginseng Radix aqua puncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. Significance was acknowledged in the effect by density difference of medicines. In the Cervus elaphus group, there was the most significant increase in density of $0.3\%$ aquapuncture, whereas in Ginseng Radix group, there was the most significant increase in density of $0.2\%$ aquapuncture. So setting up density of medicines is important and many various studies for clinical application are required.

• Journal of Food Hygiene and Safety
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• v.9 no.2
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• pp.67-74
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• 1994

This study was aimed to find out the comparative effects between non-irradiated, and 5kGy-10kGy of gamma-irradiated Panax Ginseng Radix powder on the genotoxicity for identification of possibility of DNA damage causing cancer. Four different short-term mutagenicity tests were used: (1) Salmonella typhimurium reversion assay (Ames test) (2) Chromosome aberration test in cultured Chinese hamster lung (CHL) fibroblast cells. (3) Micronucleus test in ddY mouse (4) Somatic mutation and recombination test in the wing cells of Drosophila melanogaster.Gamma-irradiated Panax Ginseng Radix powder revealed negative results in these four mutagenicity tests. This means gamma-irradiated ginseng could be safe on the genotoxic point of view.

• Journal of the East Asian Society of Dietary Life
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• v.14 no.5
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• pp.407-417
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• 2004

This study was conducted to investigate the application possibility of leaf and stem extract(LSE) extracted from the mixture of leaf and stem of ginseng radix(Panax ginseng CA. Meyer). This study measured the intake levels and efficiency ratio, growth rate, absorption ratio of carbohydrate, lipid and protein of rat by feeding with LSE during 6 weeks. We analyse the hematological and serum metabolic variables, serum lipid concentrations. Total diet and protein intake levels were low, but efficiency ratios were significantly high in LSE administered groups than the control group. Weight gain, liver and kidney weight of LSE groups were significantly higher than the control group. Blood RBC, Hct, Hb, total protein and albumin concentrations were reasonable levels in LSE administered groups compared to the basal diet group. Also serum total cholesterol, LDL-cholesterol, triglyceride contents of LSE groups were low, but HDL-cholesterol level was higher than the basal diet group. These results imply that leaf and stem of ginseng radix could be used as possible food resources, functional food material and feed stuff.

• Journal of Ginseng Research
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• v.28 no.3
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• pp.132-135
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• 2004

This study was performed to investigate the anxiolytic effects of total sponin fraction from Ginseng Radix Rubra (KRG) in mice using the elevated plus-maze model. The water extract of KRG and ginseng total saponins (GTS) purified from the water extract of KRG were administered orally to mice. One hour after administration of KRG water extract and GTS, mice were tested on the elevated plus-maze. The water extract of KRG 100 mg/kg, and GTS 25 and 50 mg/kg did not increase open arm entries and time spent on open arm. However, GTS 100 mg/kg increased the number of open arm entries and time spent on open arm. On the other hand, as the plus-maze test was affected by changes in locomotor activity, an additional test was carried out with the specific aim of monitoring locomotor activity. The water extract of KRG 100 mg/kg, and GTS 25 and 50 mg/kg did not affect the locomotor activity. However, GTS 100 mg/kg significantly decreased locomotor activity. From this study, we suggest that GTS may play an imponant role on the anxiolytic effects in the plus-maze model.

• Korean Journal of Pharmacognosy
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• v.43 no.2
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• pp.152-156
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• 2012

This study compared the contents of ginseng prosapogenin depending on the extracting conditions of Ginseng Radix palva(Panax ginseng) to provide basic information for developing Ginseng Radix palva-based functional foods. Our findings show that the content of crude saponin peaked at 18 hours of extraction and when extracted twice at $100^{\circ}C$ (GRP-18). However, the content of total saponin reached its height at 6 hours of extraction at $100^{\circ}C$ (GRP-6) and when extracted twice. On the other hand, the content of ginsenoside $Rg_3$, $Rg_5$ and $Rk_1$ from Red and Black ginseng reached their heights at 18 hours of extraction, followed by 72 hours and 15 hours of extraction at $100^{\circ}C$. And at $100^{\circ}C$ the main prosapogenin of the content of Black ginseng ginsenoside $Rg_5$ and $Rk_1$ reached their heights at 18 hours of extraction, followed by 72 hours and 15 hours of extraction.

• Microbiology and Biotechnology Letters
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• v.38 no.4
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• pp.467-474
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• 2010

Cheonggukjang is one of the traditional fermented soy-based foods in Korean diets. Studies in cell cultures, humans have revealed anti-hypertension, anti-stress, anticancer, antioxidant, immune enhancing effects. Angelica gigas, Rehmanniae radix, and Red ginseng are popular medicinal plants and widely used for oriental medicine. In this study a strategy had been developed to mobilize beneficial phenolics from Angelica gigas, Rehmanniae radix, and Red ginseng combined with fermented soy by Cheonggukjang fermentation for antioxidant and Type II diabetes management. The quality and functional characteristics of Chenggukjang fermented with Angelica gigas, Rehmanniae radix and Red ginseng. Cheonggukjang (CKJ), Angelica gigas Cheonggukjang (CKJ-DD), Rehmanniae radix Cheonggukjang (CKJ-RG), Angelica gigas and Rehmanniae radix Cheonggukjang (CKJ-DD+RG) and Red ginseng Cheonggukjang (CKJ-RED) were evaluated. The mobilized phenolic profile was evaluated for antioxidant activity and the potential to inhibit ${\alpha}$-amylase linked to hyperglycaemia. This research has important implications for the development of functional soy-based-fermented foods enriched with Angelica gigas, Rehmanniae radix and Red ginseng phenolics for oxidative stress - induced diabetic complications. Furthermore, Hunter's color values of 5 types cheonggukjang, lightness (L-values), redness (a-values) and yellowness (b-values) were evaluated. Free amino acid content of CKJ-RED (0.993 mg/gd. w.) showed higher than that of CKJ (0.205 mg/g-d.w.).