• Title/Summary/Keyword: Race 5

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Development of molecular marker to select resistant lines and to differentiate the races related to powdery mildew in melon (Cucumis melo L.) (멜론 흰가루병의 race 분화 및 저항성 계통 선발을 위한 분자마커 개발)

  • Kim, Hoy-taek;Park, Jong-in;Ishikawa, Tomoko;Kuzuya, Maki;Horii, Manabu;Yashiro, Katsutoshi;Nou, Ill-sup
    • Journal of Plant Biotechnology
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    • v.42 no.4
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    • pp.284-289
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    • 2015
  • Powdery mildew (Podosphaera xanthii) commonly occurs in cultivated fields of melon (Cucumis melo L.). It inflicts a lot of damages. Therefore, breeding resistant lines is essential. Development of a resistant line by integrating resistance gene takes a long time. In addition, break down of developed resistance by generating new virulent fungus strains increases disease susceptibility. This phenomenon was related to races of powdery mildew. Therefore, it is important to develop a DNA marker to genetically analyze race-specific resistance genes of melon powdery mildew to breed resistant lines. To date, a total of 28 races of Podosphaera xanthii have been reported in the literature. In Japan, 10 races have been reported in the Ibaraki region. We developed a system to characterize the races of Podosphaera xanthii and confirmed eight out of those 10 races in the Ibaraki region. In Korea, only one race has been characterized to date. However, some different races were detected. Through genetic analysis of resistant lines and susceptible lines of powdery mildew, resistance genes of race1 (Pm-X, PXB, and Pm-R 1), race N1 (PXA), race 2 (Pm-w and Pm-R 2), race 3 (Pm-X3), and race 5 (Pm-X5 and Pm-R5) were identified in melon. These related genes of race 1, 3, N1, 5, and race 1, 2, 5 were located at linkage group II and V, respectively. In race 1, resistance gene was located in the linkage group XII. In addition, each race-specific marker related to specific resistance gene was developed. Using race information and race selection system obtained in this study, resistant line can be bred to develop resistant cultivar for several areas. Furthermore, this will make it more easily and economically to breed resistant lines by using selected markers.

Identification of Quantitative Trait Loci for Resistance to Soybean Cyst Nematode Race 5 (콩 Cyst 선충 Race 5에 대한 저항성 QTL 탐색)

  • Choi, In-Soo;Kim, Yong-Chul;Kim, Sung-Man;Lee, Chung-Yeol;Park, Hyean-Cheal;Halina T. Skorupska
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.712-721
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    • 1997
  • The objectives of this study were; (1) to identify and localize QTLs for resistance to soybean cyst nematode(SCN) race 5 on RAPD map, (2) to idntify the magnitude and mode of inheritance for each QTL, and (3) to identify the best combinations of QTLs for resistance to SCN race 5. Based on the univariate regression analysis, we detected 26 markers(22 RAPD and 4 RFLP) which showed significant association(P<0.05) with resistance to SCN race 5. From MAPMAKER /QTL analysis, we identified two regions (LGC-20 and Group 2) for resistance to SCN race 5. The QTL that was localized at 8.0 cM from pK418C on LGC-20 showed a recessive mode of inheritance and the QTL that was localized between W03 and E02$^3$ on Group 2 showed a dominant mode of inheritance. Two pairs of flanking markers (E02$^3$ and W03, pK418C and pK418E$_1$) and one unlinked RAPD marker, G10$^1$ were used for multiple regression analysis. Marker combination which was composed of 4 markers, E02$^3$, G10$^1$, W03, and pK418E$_1$, explained the highest amount of phenotypic variation by SCN (35.2%). Further research for the identification of QTLs for resistance to SCN race 5 to explain larger portion of phenotypic variation is needed.

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Strategy for Sprint Race of Humanoid Robot (휴머노이드 로봇의 스프린트 경기 전략)

  • Gill, Woo-Ram;Ryoo, Young-Jae
    • Journal of the Korean Institute of Intelligent Systems
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    • v.26 no.5
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    • pp.390-395
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    • 2016
  • In this paper, a sprint race of human and that of humanoid robot are compared. Sprint race is one of the most popular sports games of human. HuroCup organized by FIRA is the most representative sports event of humanoid robots. The rules of the sprint race in HuroCup are explained and the strategy for the sprint race that humanoid robots can play is proposed. A child-sized humanoid robot is designed and developed so that it can play to follow the rules of the sprint race. The algorithm of the game strategy is applied to the robot, and the robot's performance is tested. Eventually, in HuroCup, it is verified that the developed humanoid robot is suitable to perform the sprint race.

The Correlation of Racing Performance with Pre-Race Blood Values in Thoroughbred Horses

  • Lee, Young-woo;Shim, Seung-tae;Song, Hee-eun;Hwang, Hye-shin;Seo, Jong-pil;Lee, Kyoung-kap
    • Journal of Veterinary Clinics
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    • v.35 no.5
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    • pp.190-194
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    • 2018
  • Recently, the horse racing industry in Korea has developed rapidly with good betting turnover. One of the most important factors in enhancing the performance of racehorses is the study of equine exercise physiology. This study was performed to analyze the results of pre-race blood test on race day and evaluate its correlation with the race performance in Thoroughbred racehorses. Twenty-one, healthy 3- to 6-year-old Thoroughbred horses were used to collect blood samples and hematological and biochemical measurements were performed. Results were analyzed to identify its correlation with racing performance. Analysis of hematological profile of pre-race blood of the racehorses revealed that red blood cell indices including RBC, Hb, and PCV levels were elevated. Additionally, levels of AP, AST, and GGT were also elevated in pre-race blood of horses on race day. RBC, Hb, and PCV levels of high performance racehorses were significantly lower than those of low class racehorses. Moreover, the analysis of correlation between blood test results and racing ability-related categories have shown negative correlations for rating, placing strike rate, prize money per race, RBC, Hb, and PCV and positive correlations for G3F and PCV. Additionally, AST value was positively correlated to prize money per race. Thus, the results of this study show that red blood cell indices and AST are good parameters for evaluating racing performance of horses before the race.

Elicitation of Indole-3-ylmethyl Glucosinolate Biosynthesis in Turnip Culture Cells and Their Relationship with Plant Resistance to Botrytis cinerea (잿빛곰팡이병 추출물을 이용한 순무배양세포의 Indole-3-ylmethyl glucosinolate의 생합성유도와 병원성연구)

  • Kwon, Soon Tae;Zhang, Vivian
    • Korean Journal of Plant Resources
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    • v.30 no.5
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    • pp.542-548
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    • 2017
  • Two different races of Botryris cinerea were selected by the response of plant leaves to the pathogen infection. Based on lesion size of the pathogen on the leaves, turnip showed susceptible response to 'Grape-01' race, and resistant to 'Orange' race. Turnip leaves infected with resistant pathogen race, "Orange", showed significantly higher content of indole-3-ylmethyl glucosinolate (I3M) than those infected with susceptible race, 'Grape-01'. Contents of I3M in the leaves with resistant 'Orange' race was 2.5 times as high as that in uninfected leaves, whereas I3M in the leaves infected with susceptible 'Grape-01' race showed lower content than in untreated leaves. Growth of turnip suspension cells was significantly inhibited by the treatment of MeOH extract or water extract of 'Orange' race as compared with the treatment of susceptible race, 'Grape-01'. Treatment of MeOH or water extract from 'Orange' race to turnip suspension cells, strongly inhibited cell viability up to 22.7% or 16.5%, respectively. However, plant cells treated with MeOH or water extract from resistant race, 'Orange' showed higher I3M content than that from susceptible race, 'Grape-01'. These results suggest that accumulation and degradation of I3M glucosinolate in turnip cells closely related to the resistance and susceptibility of turnip cells to Botrytis cinerea.

Genetic Mapping of Resistant Genes in Brassica pekinensis Against Plasmodiophora brassicae Race 6

  • Lee, Gung-Pyo;Baek, Nam-Kwon;Park, Kuen-Woo
    • The Plant Pathology Journal
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    • v.18 no.5
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    • pp.266-270
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    • 2002
  • Inbred lines of Chinese cabbage KU-101 (resistant line against Plasmodiophora brassicae race race 6) and CS-113 (susceptible line) were crossed and their progeny lines F$_1$, BC$_1$F$_1$, F$_2$, and F$_3$ were produced for the construction of the genetic linkage map of R brassicae race 6-resistant Brassica campestris ssp. pekinensis genome. Restriction fragment length polymorphism (RFLP) was applied to compare between parents and their f$_2$ progenies with a total of 192 probes and 5 restriction enzymes. The constructed RFLP map covered 1,104 cM with a mean distance between genetic marker of 8.0 cM, and produced 10 linkage groups having 121 genetic loci. The loci of P. brassicae race 6 (CR6)-resistant Brassica genome were determined by interval mapping of quan-titative trait loci (QTL), which resulted from bioassay using the same race of the fungi in P3 population. Resistant loci were estimated in numbers 1 (Gl) and 3 (G3) linkage groups. In the regression test, Gl had a value of4.8 logarithm of odd (LOD) score, while C3 had values of 4.2-7.2. Given these results, the location of the CR6-resistant loci within the Brassica genome map can now be addressed.

Analysis of Race Car Handling Characteristics Using DADS in Initial Design Step (DADS를 이용한 초기 설계 단계에서의 경기용 차량의 핸들링 특성 해석)

  • Jang, Woon-Geun
    • Journal of the Korean Society of Industry Convergence
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    • v.11 no.2
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    • pp.71-82
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    • 2008
  • In this study, 3 dimensional non-linear race car vehicle model including Chassis, steering and suspension systems were modeled by using Multi-Body Dynamics Simulation Program, DADS 9.5(Dynamic Analysis and Design System),which was used in kinematic and dynamic analysis. A full race car vehicle dynamics model using DADS program was presented and analysis was carried out to estimate the handling characteristics that may be very useful to design a race car in early design stage. The simulation of vehicle handling behavior for step steering input was simulated and compared with different design parameters: torsional stiffness of the front and rear anti roll bars, the motion ratio of the front and rear suspension system, the location of the tie rod joint, in multibody dynamic model. Therefore this simulation model before race car construction in early design step will be helpful for race car designer to save time and limited budget.

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A scheme on roundness lmprovement in internal grinding of bearing inner race (베어링 내륜의 내면 연삭가공에서 진원도 개선 방안)

  • Kim, Jeong-Suk;Kang, Myeong-Chang;Bae, Jeong-Cheol
    • Journal of the Korean Society for Precision Engineering
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    • v.13 no.5
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    • pp.60-66
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    • 1996
  • Precision of bearing race is very important to maintain the clearance between ball and inner race. In internal grinding of bearing race, its roundness is dependent on shoe wear, accuracy of jig, dressing method and grinding conditions. In this study, the characteristics of shoe wear and eccentricity of workpiece were investigated experimentally. When wear of fornt shoe wear reaches at 100.mu. m and that of rear shoe reaches at 114 .mu. m, eccentricity of inner race is increased to 1.4 .mu. m. Roundness of race is mainly related to wear of rear shoe and ring type shoe is recommended to improve roundness of race.

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Effect of Ultramarathon on the Anterior Pituitary and Thyroid Hormones (울트라마라톤이 뇌하수체 전엽 및 갑상선 호르몬에 미치는 영향)

  • Shin, Kyung-A;Kim, Young-Joo
    • The Korean journal of sports medicine
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    • v.36 no.4
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    • pp.214-220
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    • 2018
  • Purpose: The purpose of this research is to study changes in pituitary hormone in anterior lobe and thyroid hormone before, after, and during recovery time in severe 100 km ultramarathon. Methods: Healthy middle-aged runners (age, $52.0{\pm}4.8$ years) participated in the test. Grade exercise test is done, and then blood is taken from those participants before and after completing 100 km ultramarathon at the intervals of 24 hours (1 day), 72 hours (3 days), and 120 hours (5 days) to analyze their luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and free thyroxine (Free T4). Results: For LH, it decreased more significantly at 100 km than pre-race. However, after 1 day result increased more than that of 100 km. At 3 days, it was significantly higher than pre-race and 100 km, recovering at 5 days. In terms of FSH, it decreased at 100 km, 1 day, and 3 days more than pre-race but recovered at 5 days. TSH was higher at 1 day and 5 days compared to pre-race. T3 was only higher at 100 km than pre-race. T4 was higher till 5 days at 100 km than pre-race. Free T4 increased more significantly at 100 km than pre-race. Conclusion: In terms of severe long distance running, LH and FSH which belong to hormone from anterior lobe as well as T3, T4, and Free T4 which belong to thyroid hormone showed their variation within the standard range. However, TSH showed abnormal increase from enhanced concentration of blood after marathon becoming hyper-activation even during the recovery period.

Cardiac Damage Biomarkers Following a Triathlon in Elite and Non-elite Triathletes

  • Park, Chan-Ho;Kim, Kwi-Baek;Han, Jin;Ji, Jin-Goo;Kwak, Yi-Sub
    • The Korean Journal of Physiology and Pharmacology
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    • v.18 no.5
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    • pp.419-423
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    • 2014
  • The purpose of the present study was to investigate cardiac damage biomarkers after a triathlon race in elite and non-elite athlete groups. Fifteen healthy men participated in the study. Based on performance, they were divided into elite athlete group (EG: n=7) and non-elite athlete group (NEG: n=8). Participants' blood samples were obtained during four periods: before, immediately, 2 hours and 7 days after finishing the race. creatine kinase (CK), creatine kinase-myoglobin (CK-MB), myoglobin, and lactate dehydrogenase (LDH) were significantly increased in both groups immediately after, and 2 hours after finishing the race (p<.05). CK, CK-MB, and myoglobin were completely recovered after 7 days (p<.05). Hematocrit (Hct) was significantly decreased in both groups (p<.05) 7 days after the race. LDH was significantly decreased in the EG (p<.05) only 7 days after the race. Homoglobin (Hb) was significantly decreased in the NEG (p<.05) only 2 hours after the race. Although cardiac troponin T (cTnT) was significantly increased in the EG but not in the NEG 2hours after the race (p<.05), there was no group-by-time interaction. cTnT was completely recovered in both groups 7 days after the race. In conclusion, cardiac damage occurs during a triathlon race and, is greater in elite than in non-elite. However, all cardiac damage markers return to normal range within 1 week.