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http://dx.doi.org/10.7732/kjpr.2017.30.5.542

Elicitation of Indole-3-ylmethyl Glucosinolate Biosynthesis in Turnip Culture Cells and Their Relationship with Plant Resistance to Botrytis cinerea  

Kwon, Soon Tae (Department of Horticulture and Breeding, Andong National University)
Zhang, Vivian (Department of Plant Science, University of California)
Publication Information
Korean Journal of Plant Resources / v.30, no.5, 2017 , pp. 542-548 More about this Journal
Abstract
Two different races of Botryris cinerea were selected by the response of plant leaves to the pathogen infection. Based on lesion size of the pathogen on the leaves, turnip showed susceptible response to 'Grape-01' race, and resistant to 'Orange' race. Turnip leaves infected with resistant pathogen race, "Orange", showed significantly higher content of indole-3-ylmethyl glucosinolate (I3M) than those infected with susceptible race, 'Grape-01'. Contents of I3M in the leaves with resistant 'Orange' race was 2.5 times as high as that in uninfected leaves, whereas I3M in the leaves infected with susceptible 'Grape-01' race showed lower content than in untreated leaves. Growth of turnip suspension cells was significantly inhibited by the treatment of MeOH extract or water extract of 'Orange' race as compared with the treatment of susceptible race, 'Grape-01'. Treatment of MeOH or water extract from 'Orange' race to turnip suspension cells, strongly inhibited cell viability up to 22.7% or 16.5%, respectively. However, plant cells treated with MeOH or water extract from resistant race, 'Orange' showed higher I3M content than that from susceptible race, 'Grape-01'. These results suggest that accumulation and degradation of I3M glucosinolate in turnip cells closely related to the resistance and susceptibility of turnip cells to Botrytis cinerea.
Keywords
Botrytis cinerea; Indole-3-ylmethyl glucosinolate; Resistance; Turnip;
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