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http://dx.doi.org/10.5423/PPJ.2002.18.5.266

Genetic Mapping of Resistant Genes in Brassica pekinensis Against Plasmodiophora brassicae Race 6  

Lee, Gung-Pyo (Institute of Natural Science, Seoul Women′s University)
Baek, Nam-Kwon (Samsung Breeding Co.)
Park, Kuen-Woo (Division of Bioscience and Technology, College of Life and Environment Sciences, Korea University)
Publication Information
The Plant Pathology Journal / v.18, no.5, 2002 , pp. 266-270 More about this Journal
Abstract
Inbred lines of Chinese cabbage KU-101 (resistant line against Plasmodiophora brassicae race race 6) and CS-113 (susceptible line) were crossed and their progeny lines F$_1$, BC$_1$F$_1$, F$_2$, and F$_3$ were produced for the construction of the genetic linkage map of R brassicae race 6-resistant Brassica campestris ssp. pekinensis genome. Restriction fragment length polymorphism (RFLP) was applied to compare between parents and their f$_2$ progenies with a total of 192 probes and 5 restriction enzymes. The constructed RFLP map covered 1,104 cM with a mean distance between genetic marker of 8.0 cM, and produced 10 linkage groups having 121 genetic loci. The loci of P. brassicae race 6 (CR6)-resistant Brassica genome were determined by interval mapping of quan-titative trait loci (QTL), which resulted from bioassay using the same race of the fungi in P3 population. Resistant loci were estimated in numbers 1 (Gl) and 3 (G3) linkage groups. In the regression test, Gl had a value of4.8 logarithm of odd (LOD) score, while C3 had values of 4.2-7.2. Given these results, the location of the CR6-resistant loci within the Brassica genome map can now be addressed.
Keywords
Brassica pekinensis; clubroot; genetic mapping; Plasmodiophora brassicae; QTL;
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