• 대한화장품학회지
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• 제31권2호
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• pp.161-167
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• 2005

잎새버섯(Grifola frondosa HB0071)이 생산하는 세포외 다당체인 GF-glucan이 사람섬유아세포에서 자외선(UVA)조사 시 증가되는 MMP-1 발현에 미치는 영향을 조사하였다. 자외선으로부터 조사된 섬유아세포에 GF-glucan을 처리한 농도에 따라 MMP-1 발현이 억제되었으며, RT-PCR를 이용해 세포내 MMP-1 mRNA 발현 또한 감소하는 것으로 나타났다 즉, 최대 GF-glucan $0.5\%$를 처리했을 때 $54.4\%$의 MMP-1 발현을 억제하였다. 결과적으로, 잎새버섯 HB0071로부터 생산된 GF-glucan은 피부노화와 관련된 extracellular matrix (ECM) 조직에 손상을 주는 MMP-1의 활성을 억제하여 자외선으로부터 손상된 피부의 광노화로부터 보호해주는 것으로 밝혀졌다.

• 미생물학회지
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• 제54권2호
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• pp.161-163
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• 2018

대한민국 남해에서 채집한 해조류 감태(Ecklonia cava)로부터 분리한 Flavivirga eckloniae $ECD14^T$ 균주의 유전체서열을 분석하였다. 균주 $ECD14^T$의 유전체는 G + C 비율이 33.9%이며, 4,647개의 유전자와 4,595개의 단백질 코딩 유전자, 44개의 위유전자, 52개의 RNA 유전자를 포함한 단일 원형 염색체로 구성되었으며 그 크기는 2,371,912 bp였다. 파아지와 트랜스포존 유전자가 존재하며, CISPR array 관련 유전자 및 서열은 발견되지 않았다. 균주 $ECD14^T$는 해조 다당의 분해에 관여하는 alginate lyase와 ${\beta}$-glucosidase 유전자를 가지고 있었다.

• 미생물학회지
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• 제53권4호
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• pp.351-353
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• 2017

토마토 뿌리에서 분리한 Chryseobacterium sp. T16E-39 균주는 식물생육촉진과 역병, 시들음병에 대한 억제효과가 있었다. 이 균주의 유전체 염기서열은 4,873,888 염기쌍이었으며 G + C 함량은 35.22%이었다. 이 유전체는 4,289개 단백질 유전자, 15개 rRNA 유전자, 71개 tRNA 유전자를 포함하였다. T16E-39 균주의 유전체에서 인산가용화, 식물호르몬 조절, 항산화 활성, 키틴 분해, 제9형 분비시스템에 관여하는 유전자를 확인하였으며, 이들 유전자는 식물의 생육을 촉진하고 병발생을 억제하는 기작과 관련되어 있을 것으로 판단된다.

• 한국응용과학기술학회지
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• 제38권5호
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• pp.1229-1240
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• 2021

은행나무 종자인 백과를 70% 에탄올로 추출하여 미백 효능을 규명하고 화장품 소재로서의 응용 가능성을 확인하고자 하였다. 백과 에탄올추출물 (GBE)을 B16F10 melanoma cells에 처치하여 멜라닌 생성과 tyrosinase 활성을 확인한 결과 유의한 수준의 멜라닌 생성 저해가 관찰되었고, 멜라닌 생성과정에 관여하는 주요 효소인 tyrosinase의 활성이 농도 의존적으로 억제됨이 관찰되었다. 멜라닌 생성 관련 주요 인자들의 단백질 발현과 mRNA 수준을 관찰한 결과, tyrosinase, tyrosinase-related protein-1, -2 (TRP-1, -2), microphthalmia-associated transcription factor (MITF)의 단백질 발현과 유전자 수준이 GBE의 처리에 의해 유의한 수준으로 저해되었다. 이 결과를 통해, 본 연구의 백과 에탄올추출물은 멜라닌 세포의 멜라닌 생성 관련 핵심 전사인자인 MITF의 조절을 통해 멜라닌 생성 억제 효과를 나타내는 것으로 보인다. 이에 따라 백과 에탄올추출물을 화장품 미백 기능성 소재로 활용 가능성이 있을 것으로 기대된다.

• 미생물학회지
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• 제55권3호
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• pp.248-257
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• 2019

본 연구에서는 제주 지역의 토양 및 해양 환경으로부터 약 70주의 미생물들을 분리하였으며, 16S ribosomal RNA 유전자 분석을 통하여 최종 21종의 미생물을 발굴하였다. 이들 미생물들은 5 강(Class) 16 속(Genus)에 속하며, 모두 국내 미기록종으로 확인되었다. 분리된 미생물의 기질 특이성 및 고분자 물질 분해능을 바탕으로 내산성과 호염성 미생물들의 생리활성 표현형은 서로 구별되는 것으로 관찰되었다. 본 연구결과는, 국내 미생물 자원활용에 기초적 정보를 제공할 것으로 기대된다.

• 한국미생물·생명공학회지
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• 제49권3호
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• pp.432-439
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• 2021

Microbial community plays important roles in the culture environment of mud crab Scylla serrata. One of the environmental management efforts for the cultivation of S.serrata is by stabilizing microorganisms involved in nitrogen cycle process. The availability of dissolved inorganic nitrogen in its culture environment under a recirculating system closely relates to the nitrogen cycle, which involves both anaerobic and aerobic bacterial activities. Anaerobically, there are two major nitrogen compound degradation processes, i.e., denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This study aimed to identify denitrifying and DNRA bacteria isolated from the recirculating cultivation of S. serrata. The water samples were collected from anaerobic filters called close filter system, which is anaerobically conditioned with the addition of varying physical filter materials in the recirculating mud crab cultures. The results showed that three denitrifying bacterial isolates and seven DNRA bacterial isolates were successfully identified. The phylogenetic analysis based on 16S rRNA gene of the denitrifying bacteria revealed that HIB_7a had the closest similarity to Stenotrophomonas daejeonensis strain MJ03. Meanwhile, DNRA bacterial isolate of HIB_92 showed a 100% similarity to Bacillus sonorensis strain N3, Bacillus vallismortis strain VITS-17, Bacillus tequlensis strain TY5, Geobacillus sp. strain DB24, Bacillus subtilis strain A1, and Bacillus mojavensis strain SSRAI21. This study provides basic information denitrifying and DNRA bacterial isolates identity which might have the potential to be applied as probiotics in aquaculture systems in order to maintain optimal environmental conditions.

• Biomolecules & Therapeutics
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• 제30권6호
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• pp.540-545
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• 2022

Betulin is a triterpenoid natural product contained in several medicinal plants including Betulae Cortex. These medicinal plants have been used for controlling diverse inflammatory diseases in folk medicine and betulin showed anti-inflammatory, antioxidative, and anticancer activities. In this study, we tried to examine whether betulin exerts a regulative effect on the gene expression of MUC5AC mucin under the status simulating a pulmonary inflammation, in human airway epithelial cells. Confluent NCI-H292 cells were pretreated with betulin for 30 min and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 h or the indicated periods. The MUC5AC mucin mRNA expression and mucin glycoprotein production were measured by reverse transcription - polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. To elucidate the action mechanism of betulin, effect of betulin on PMA-induced nuclear factor kappa B (NF-kB) signaling pathway was also investigated by western blot analysis. The results were as follows: 1) Betulin significantly suppressed the production of MUC5AC mucin glycoprotein and down-regulated MUC5AC mRNA expression induced by PMA in NCI-H292 cells. 2) Betulin inhibited NF-κB activation stimulated by PMA. Suppression of inhibitory kappa B kinase (IKK) by betulin led to the inhibition of the phosphorylation and degradation of inhibitory kappa B alpha (IκBα), and the nuclear translocation of NF-κB p65. This, in turn, led to the down-regulation of MUC5AC glycoprotein production in NCI-H292 cells. These results suggest betulin inhibits the gene expression of mucin through regulation of NF-kB signaling pathway, in human airway epithelial cells.

• Animal Bioscience
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• 제34권9호
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• pp.1439-1450
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• 2021

Objective: With the rapid development of proteomics sequencing and RNA sequencing technology, multi-omics analysis has become a current research hotspot. Our previous study indicated that Xinjiang brown cattle have better meat quality than Kazakh cattle. In this study, Xinjiang brown cattle and Kazakh cattle were used as the research objects. Methods: Proteome sequencing and RNA sequencing technology were used to analyze the proteome and transcriptome of the longissimus dorsi muscle of the two breeds of adult steers (n = 3). Results: In this project, 22,677 transcripts and 1,874 proteins were identified through quantitative analysis of the transcriptome and proteome. By comparing the identified transcriptome and proteome, we found that 1,737 genes were identified at both the transcriptome and proteome levels. The results of the study revealed 12 differentially expressed genes and proteins: troponin I1, crystallin alpha B, cysteine, and glycine rich protein 3, phosphotriesterase-related, myosin-binding protein H, glutathione s-transferase mu 3, myosin light chain 3, nidogen 2, dihydropyrimidinase like 2, glutamate-oxaloacetic transaminase 1, receptor accessory protein 5, and aspartoacylase. We performed functional enrichment of these differentially expressed genes and proteins. The Kyoto encyclopedia of genes and genomes results showed that these differentially expressed genes and proteins are enriched in the fatty acid degradation and histidine metabolism signaling pathways. We performed parallel reaction monitoring (PRM) verification of the differentially expressed proteins, and the PRM results were consistent with the sequencing results. Conclusion: Our study provided and identified the differentially expressed genes and proteins. In addition, identifying functional genes and proteins with important breeding value will provide genetic resources and technical support for the breeding and industrialization of new genetically modified beef cattle breeds.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.261-261
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• 2022

Cowpea [Vigna unguiculata (L.) Walp] is one of the most important grain legumes that enhance soil fertility and is well-adapted to various abiotic stress. Also, it is cultivated worldwide as a tropical annual crop, and the semi-arid regions are known as the main cowpea-produced regions. However, accumulation of soil salinity induced by low rainfall in these regions is reducing crop yields and quality. In general, plants exposed to soil salinity cause an accumulation of high ion chloride, which leads to the degradation of root and leaf proteins. In this study, we identified candidate genes associated with salinity tolerance through an analysis of differentially expressed genes (DEGs) in four cowpea germplasms with contrasting salinity tolerance. A total of 553,776,035 short reads were obtained using the Illumina Novaseq 6000 platform for RNA-Seq, which were subsequently aligned to the reference genome of cowpea Vunguiculata v1.2. A total of9,806 DEGs were identified between NaCl treatment and control of four cowpea germplasms. Among these DEGs, functions related to salt stress such as calcium transporter and cytochrome-450 family were associated with salt stress. In GO analysis and KEGG analysis, these DEGs were enriched in terms such as the "phosphorylation", ''extracellular region", and "ion binding". These RNA-seq results will improve the understanding of the salt tolerance of cowpea and can be used as useful basic data for molecular breeding technology in the future.

• 동의생리병리학회지
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• 제22권6호
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• pp.1572-1578
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• 2008

In previous report, Seungmagalgeun-tang (SGT) could exert its anti-inflammatory actions in the BV-2 microglial cells. However, study on the anti-inflammatory effect of SGT in mast cells has not been identified. Therefore, we examined on the anti-inflammatory effect of SGT on the phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-induced rat basophilic leukemia (RBL-2H3) cells. SGT inhibited the release of ${\beta}$-hexosaminidase and secretion and expression of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-4 on RBL-2H3 cells, without affecting cell viability. The protein expression level of nuclear factor (NF)-${\kappa}B$ (p65) was decreased in the nucleus by SGT. In addition, SGT suppressed the degradation of inhibitory protein $I{\kappa}B-{\alpha}$ protein, the activation of p38 mitogen-activated protein kinase (MAPK), and the expressions of cyclooxygenase (COX)-2 mRNA and protein level in RBL-2H3 cells. These results suggest that SGT could be involved anti-allergic effect by control of NF-${\kappa}B$ (p65) translocation into the nucleus through inhibition of $I{\kappa}B-{\alpha}$ degradation and suppression of COX-2 expression.