Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Combined transcriptome and proteome analyses reveal differences in the longissimus dorsi muscle between Kazakh cattle and Xinjiang brown cattle

  • Yan, XiangMin (Institute of Animal Husbandry, Xinjiang Academy of Animal Husbandry) ;
  • Wang, Jia (College of Geographic Science, Shanxi Normal University) ;
  • Li, Hongbo (Institute of Animal Husbandry, Xinjiang Academy of Animal Husbandry) ;
  • Gao, Liang (Yili Vocational and Technical College) ;
  • Geng, Juan (Xinjiang Animal Husbandry General Station) ;
  • Ma, Zhen (Institute of Animal Husbandry, Xinjiang Academy of Animal Husbandry) ;
  • Liu, Jianming (Yili Animal Husbandry General Station) ;
  • Zhang, Jinshan (Institute of Animal Husbandry, Xinjiang Academy of Animal Husbandry) ;
  • Xie, Penggui (Yili Vocational and Technical College) ;
  • Chen, Lei (College of Animal Science and Technology, Shihezi University)
  • Received : 2020.10.31
  • Accepted : 2021.01.29
  • Published : 2021.09.01
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Abstract

Objective: With the rapid development of proteomics sequencing and RNA sequencing technology, multi-omics analysis has become a current research hotspot. Our previous study indicated that Xinjiang brown cattle have better meat quality than Kazakh cattle. In this study, Xinjiang brown cattle and Kazakh cattle were used as the research objects. Methods: Proteome sequencing and RNA sequencing technology were used to analyze the proteome and transcriptome of the longissimus dorsi muscle of the two breeds of adult steers (n = 3). Results: In this project, 22,677 transcripts and 1,874 proteins were identified through quantitative analysis of the transcriptome and proteome. By comparing the identified transcriptome and proteome, we found that 1,737 genes were identified at both the transcriptome and proteome levels. The results of the study revealed 12 differentially expressed genes and proteins: troponin I1, crystallin alpha B, cysteine, and glycine rich protein 3, phosphotriesterase-related, myosin-binding protein H, glutathione s-transferase mu 3, myosin light chain 3, nidogen 2, dihydropyrimidinase like 2, glutamate-oxaloacetic transaminase 1, receptor accessory protein 5, and aspartoacylase. We performed functional enrichment of these differentially expressed genes and proteins. The Kyoto encyclopedia of genes and genomes results showed that these differentially expressed genes and proteins are enriched in the fatty acid degradation and histidine metabolism signaling pathways. We performed parallel reaction monitoring (PRM) verification of the differentially expressed proteins, and the PRM results were consistent with the sequencing results. Conclusion: Our study provided and identified the differentially expressed genes and proteins. In addition, identifying functional genes and proteins with important breeding value will provide genetic resources and technical support for the breeding and industrialization of new genetically modified beef cattle breeds.

Keywords

Acknowledgement

This study was supported by the Xinjiang autonomous region basic research fee in 2021, the autonomous region modern livestock and poultry seed industry promotion special fund in 2021 (2021XJHN), the Xinjiang autonomous region basic research fee in 2019 (KY2019117), the modern agricultural industrial technology system (CARS-37) and the study on school-level project (high-level talent research start-up project, 20200162).

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