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http://dx.doi.org/10.7845/kjm.2019.9043

Isolation and characterization of acid-resistanct and halophilic bacteria using cultivation technique in Jeju island  

Han, Bit (Department of Biology, Jeju National University)
Kim, Minji (Department of Biology, Jeju National University)
Ryu, Dajung (Department of Biology, Jeju National University)
Lee, Ki-Eun (Microorganism Resources Division, National Institute of Biological Resources)
Lee, Byoung-Hee (Microorganism Resources Division, National Institute of Biological Resources)
Lee, Eun-Young (Exhibition & Education Division, National Institute of Biological Resources)
Park, Soo-Je (Department of Biology, Jeju National University)
Publication Information
Korean Journal of Microbiology / v.55, no.3, 2019 , pp. 248-257 More about this Journal
Abstract
In this study, we isolated about 70 bacterial strains from terrestrial and marine environments in Jeju island, and finally, total 21 strains were obtained based on the 16S ribosomal RNA gene sequence analysis. These isolated strains were classified into 16 genera of 5 classes and were identified as an unrecorded species in the Republic of Korea. As a result of the substrate utilization and capability for polymer degradation, the physiological phenotypes for acid resistance and halophilic bacteria were observed to be distinct from each other, except for some acid resistance strains. This study might provide basic information on utilization for indigenous microorganisms.
Keywords
Jeju; acidophiles; bacteria; halophiles; marine sediment; soil;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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1 Choi H, Koh HW, Kim H, Chae JC, and Park SJ. 2016. Microbial community composition in the marine sediments of Jeju Island: Next-generation sequencing surveys. J. Microbiol. Biotechnol. 26, 883-890.   DOI
2 Han S, Cho M, and Whang KS. 2008. Comparison of phylogenetic characteristics of bacterial populations in a quercus and pin humus forest soil. Korean J. Microbiol. 44, 237-243.
3 Huang Q, Kaiser K, and Benner R. 2012. A simple high performance liquid chromatography method for the measurement of nucleobases and the RNA and DNA content of cellular material. Limnol. Oceanogr-Meth. 10, 608-616.   DOI
4 Kallmeyer J, Pockalny R, Adhikari RR, Smith DC, and D'Hondt S. 2012. Global distribution of microbial abundance and biomass in subseafloor sediment. Proc. Natl. Acad. Sci. USA 109, 16213-16216.   DOI
5 KFRI. 2014. Ecology and Culture on the Gotjawal (Lava Forests). Korea Forest Research Institute.
6 Kim YE, Koh HW, Kim SJ, Do KT, and Park SJ. 2017. Isolation and characterization in the exhausted mine and Jeju Gotjawal. Korean J. Microbiol. 53, 309-315.   DOI
7 Koh HW, Hong H, Min UG, Kang MS, Kim SG, Na JG, Rhee SK, and Park SJ. 2015a. Rhodanobacter aciditrophus sp. nov., an acidophilic bacterium isolated from mine wastewater. Int. J. Syst. Evol. Microbiol. 65, 4574-4579.   DOI
8 Koh HW, Kim SJ, Rhee SK, and Park SJ. 2015b. Isolation and characterization analysis of the halophilic archaea isolated from solar saltern, Gomso. Korean J. Microbiol. 51, 427-434.   DOI
9 Locey KJ and Lennon JT. 2016. Scaling laws predict global microbial diversity. Proc. Natl. Acad. Sci. USA 113, 5970-5975.   DOI
10 NIBR. 2013. Studies on Species Diversity of Microorganisms in Mountain Wetlands and Gotjawal. National Institute of Biological Resources.
11 Overmann J, Abt B, and Sikorski J. 2017. Present and future of culturing bacteria. Annu. Rev. Microbiol. 71, 711-730.   DOI
12 Whitman WB, Coleman DC, and Wiebe WJ. 1998. Prokaryotes: The unseen majority. Proc. Natl. Acad. Sci. USA 95, 6578-6583.   DOI
13 Scofield V, Jacques SM, Guimaraes JR, and Farjalla VF. 2015. Potential changes in bacterial metabolism associated with increased water temperature and nutrient inputs in tropical humic lagoons. Front. Microbiol. 6, 310.   DOI