• Title/Summary/Keyword: RISA

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Preparation of Radiopharmaceutical (II) (방사성의약품(放射性醫藥品) 합성방식(合成方式)에 관(關)한 연구(硏究) -제 II 보-(第 II 報))

  • Kim, You-Sun;Kim, Tae-Young;Uhm, Kyung-Ja
    • The Korean Journal of Nuclear Medicine
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    • v.2 no.1
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    • pp.67-71
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    • 1968
  • Mercury bromo hydroxy propane-$Hg^{203},\;Tc-^{99m}$ colloidal, $Tc-^{99m}$ per technate (from $Tc-^{99m}$ Generator) were prepared and prepared products were tested their clinical usability to give agreeable results. Labelling of HSA by $Tc^{99m}$ and coagulation of HSA to MAA were conducted on a lab. scale. The iodine containning Radiopharmaceuticals; Hippuran, Sodiumiodide (inj), RISA, MAA, Rosebengal, Triolein, Phosphorus protein and $Mercury^{203}$ neohydrine were prepared by the conventional procedure. Total 206. 4 mC of Radiopharmaceuticals were prepared and 141.910 mC of them were distributed to the major users in this country.

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Molecular detection of bat coronaviruses in three bat species in Indonesia

  • Dharmayanti, Ni Luh Putu Indi;Nurjanah, Diana;Nuradji, Harimurti;Maryanto, Ibnu;Exploitasia, Indra;Indriani, Risa
    • Journal of Veterinary Science
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    • v.22 no.6
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    • pp.70.1-70.12
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    • 2021
  • Bats are an important reservoir of several zoonotic diseases. However, the circulation of bat coronaviruses (BatCoV) in live animal markets in Indonesia has not been reported. Genetic characterization of BatCoV was performed by sequencing partial RdRp genes. Real-time polymerase chain reaction based on nucleocapsid protein (N) gene and Enzyme-linked immunosorbent assay against the N protein were conducted to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA and antibody, respectively. We identified the presence of BatCoV on Cynopterus brachyotis, Macroglossus minimus, and Rousettus amplexicaudatus. The results showed that the BatCoV included in this study are from an unclassified coronavirus group. Notably, SARS-CoV-2 viral RNA and antibodies were not detected in the sampled bats.

Newcastle disease virus: the past and current situation in Indonesia

  • NLP Indi Dharmayanti;Diana Nurjanah;Harimurti Nuradji;Teguh Suyatno;Risa Indriani
    • Journal of Veterinary Science
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    • v.25 no.1
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    • pp.3.1-3.20
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    • 2024
  • The Newcastle disease virus (NDV) outbreak was first reported in Java Island, Indonesia, in 1926, which was then reported further in Newcastle-upon-Tyne, England. Nevertheless, the NDV is still endemic in Indonesia, with outbreaks occurring in free-range and commercial chicken farms. The dynamic evolution of the NDV has led to the further development of vaccines and diagnostic tools for more effective control of this virus. This paper discusses the history of the NDV occurrence, vaccines, the development of diagnostic tools, and the epidemiological condition of the NDV in Indonesia. Indonesia, which has the largest poultry population in the world after China, has challenges in preventing and controlling this virus that causes economic losses to the farmers and has an impact on the welfare of the poultry farming community in Indonesia.

Correlation Analysis of UA Using Wind Data of AWS/ASOS and SST in Summer in the East Sea (AWS/ASOS 바람자료를 이용한 여름철 동해 연안역의 용승지수와 수온과의 상관성)

  • Kim, Ju-Yeon;Han, In-Seong;Ahn, Ji-Suk
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.24 no.6
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    • pp.773-784
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    • 2018
  • In this study, we examined the UA (upwelling age) using wind data of AWS/ASOS in the East Sea coast and the correlation between UA and SST (sea surface temperature) from May to August in 1995 to 2016. The data used the 6 observations of the wind data of AWS/ASOS and the SST data of the COD/RISA provided by the National Institute and Fisheries Science near the East Sea coast. The UA was calculated quantitatively low but it rose when the actual cold water mass occurred. Correlation analysis between UA and SST showed the negative (-) r (correlation coefficient) predominately. At the time of cold-water mass in June to August 2013, the r had a very high negative value of -0.65 to -0.89 in the 6 observations. It proved that as the UA increases, the SST is lower. By knowing the UA, we were able to evaluate the trend of upwelling in the cold-water mass of the East Sea coast in the long term and it will contribute to minimizing the damage to aquatic organisms according to the size and intensity of the upwelling.

In vitro anthelmintic evaluation of common Indian Ayurvedic anthelmintic drugs: Krimimudgar Ras, Kriminol and Vidangasava against intestinal helminths

  • Chen, Risa Parkordor;Yadav, Arun K.
    • CELLMED
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    • v.8 no.3
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    • pp.15.1-15.5
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    • 2018
  • Helminthiasis is one of the most common worm diseases which causes a range of adverse health problems in humans. Ayurveda is one of the most prominent and ancient systems of traditional medicines in India. Most Ayurvedic drugs used against intestinal helminths have been developed by traditional wisdom and therefore lack a proper validation through controlled studies. The aim of the present study was to scientifically validate the in vitro efficacy of three common Indian Ayurvedic anthelmintic drugs, viz. Krimimudgar Ras, Kriminol and Vidangasava in relation to the synthetic broad-spectrum anthelmintic drugs, praziquantel and albendazole. The in vitro testing of Ayurvedic anthelmintics was done against an intestinal cestode, Raillietina sp. and a nematode, Syphacia obvelata, employing 10 mg/ml, 30 mg/ml and 50 mg/ml concentrations of each medicine. The anthelmintic efficacy was judged on the basis of paralysis and mortality time of worms after exposing to these Ayurvedic drugs. Of the three tested Ayurvedic medicines, Krimimudgar ras (KR) showed the most prominent efficacy, against both the cestode and nematode parasites. At 50 mg/ml concentration, KR caused mortality of cestodes in $7.53{\pm}0.15hr$, and of nematodes in $7.61{\pm}0.19hr$. Vidangasava was found to be comparatively less effective against the tested helminth parasites. The results of this study indicate that Ayurvedic formulations do possess significant anthelmintic effects, however, an evidence-based research is required to validate all currently used Ayurvedic anthelmintics, using proper controlled studies.

Cultivation-Dependent and -Independent Characterization of Microbial Community Producing Polyhydroxyalkanoates from Raw Glycerol

  • Ciesielski, Slawomir;Pokoj, Tomasz;Klimiuk, Ewa
    • Journal of Microbiology and Biotechnology
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    • v.20 no.5
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    • pp.853-861
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    • 2010
  • High substrate costs decrease the profitability of polyhydroxyalkanoates (PHAs) production, and thus low-cost carbon substrates coming from agricultural and industrial residuals are tested for the production of these biopolymers. Among them, crude glycerol, formed as a by-product during biodiesel production, seems to be the most promising source of carbon. The object of this study was to characterize the mixed population responsible for the conversion of crude glycerol into PHAs by cultivation-dependent and -independent methods. Enrichment of the microbial community was monitored by applying the Ribosomal Intergenic Spacer Analysis (RISA), and the identification of community members was based on 16S rRNA gene sequencing of cultivable species. Molecular analysis revealed that mixed populations consisted of microorganisms affiliated with four bacterial lineages: ${\alpha}$, ${\gamma}$-Proteobacteria, Actinobacteria, and Bacteroides. Among these, three Pseudomonas strains and Rhodobacter sp. possessed genes coding for polyhydroxyalkanoates synthase. Comparative analysis revealed that most of the microorganisms detected by direct molecular analysis were obtained by the traditional culturing method.

Prediction of Bending Fatigue Life of Cracked Out-of-Plane Gusset Joint Repaired by CFRP Plates

  • Matsumoto, Risa;Komoto, Takafumi;Ishikawa, Toshiyuki;Hattori, Atsushi;Kawano, Hirotaka
    • International journal of steel structures
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    • v.18 no.4
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    • pp.1284-1296
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    • 2018
  • Carbon fiber reinforced polymer (CFRP), plates bonding repair method is one of the simple repair methods for cracked steel structures. In this study, the influence of width of CFRP plates on bending fatigue life of out-of-plane gusset joint strengthened with CFRP plates was investigated from the experimental and numerical point of view. In the bending fatigue test of cracked out-of-plane gusset joint strengthened with CFRP plates, the effect of width of CFRP plates on crack growth life was clarified experimentally. Namely, it was revealed that the crack growth life becomes larger with increasing the width of CFRP plates. In the numerical approach, the stress intensity factor (SIF) at the surface point of a semi-elliptical surface crack was estimated based on the linear fracture mechanics. Furthermore, the extended fatigue life of cracked out-of-plane gusset joint strengthened with CFRP plates was evaluated by using the estimated SIF at the surface point and the empirical formula of the aspect ratio of semi-elliptical crack. As the results of numerical analysis, the estimated fatigue life of the specimen strengthened with CFRP plates showed the good agreement with the test results.

Factors Affecting Work Performance During the COVID-19 Pandemic: An Empirical Study from Indonesia

  • SUPANTO, Fajar;LEGOWO, Ignatius Bendu Risa Putra;FIRDAUS, Muhammad Rizki
    • The Journal of Asian Finance, Economics and Business
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    • v.9 no.7
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    • pp.145-152
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    • 2022
  • The purpose of this study is to assess the impact of the principal's democratic leadership style, teacher competency, work discipline, and work environment on teacher performance during the pandemic. Using the proportional random sampling strategy, a sample of 468 respondents consisted of kindergarten teachers, elementary school teachers, junior high school teachers, junior high school teachers, and high school/vocational school teachers. The study revealed that the principal's democratic leadership style, teacher competence, work discipline, and work environment substantially impact teacher performance. However, the principal's democratic leadership style does not affect teacher performance, whereas teacher competence, work discipline, and work environment have a minor impact on teacher performance. Furthermore, during the COVID-19 pandemic, work discipline is the most critical variable influencing teacher performance. The findings of this study suggest that the principal's democratic leadership style, teacher competence, work discipline, and work environment have a positive impact on teacher performance during the pandemic. During the COVID-19 pandemic, work discipline is the most important variable influencing teacher performance. Considering that democratic leadership has no effect on teacher performance and that this leadership style is widely used by school principals in the world of education, it is assumed that there is no effect on teacher performance.

Scanning electron microscopic effects of Indian Ayurvedic drugs: Krimimudgar Ras, Kriminol and Vidangasava on a tapeworm, Raillietina sp.

  • Chen, Risa Parkordor;Yadav, Arun K.
    • CELLMED
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    • v.9 no.4
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    • pp.9.1-9.4
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    • 2019
  • Ayurveda is one of the ancient systems of traditional medicine in India, however, there is a lack of proper scientific research and controlled studies on efficacy and toxicity of several Ayurvedic formulations using the methods of modern science, which hinders the proper development of Ayurveda, both in India and abroad. The present study was undertaken to further corroborate the efficacy of some popular anthelmintic Ayurvedic medicines, as evident by the pharmacological effects of Ayurvedic formulations on body surface of a cestode parasite. This study employed a common intestinal cestode of domestic fowl, Raillietina sp., as a model parasite, that was exposed in vitro to different concentrations of three common Indian Ayurvedic anthelmintic drugs, namely Krimimudgar Ras, Kriminol and Vidangasava, and also to a modern anthelmintic drug, praziquantel (PZQ). After the paralysis of parasites, the specimens from the highest drug-treated concentration (50 mg/ml), praziquantel (1 mg/ml), and control groups, were collected and processed for scanning electron microscopic studies to document the effects of drugs on the body surface of parasite. In this study, maximum degree of alterations on parasite body surface was observed in the worms exposed to Krimimudgar Ras, indicating this drug to be highly efficacious against intestinal worms and brings out a transtegumental mode of action on parasite. In conclusion, the findings of present study suggest that of the three Ayurvedic formulations tested, Krimimudgar Ras possesses significant anthelmintic effects, which are mediated through parasite's body surface. Therefore, Krimimudgar Ras may be considered as a reliable anthelmintic Ayurvedic formulation, as validated through this study.

Analysis of Genomic Structure of an Aflatoxin Biosynthesis Homologous Gene Cluster in Aspergillus oryzae RIB Strains

  • Lee, Yun-Hae;Tominaga, Mihoko;Hayashi, Risa;Sakamoto, Kazutoshi;Yamada, Osamu;Akita, Osamu
    • 한국균학회소식:학술대회논문집
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    • 2006.10a
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    • pp.32-44
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    • 2006
  • To investigate non-aflatoxin-production of A. oryzae at the molecular level, an aflatoxin biosynthesis gene homolog cluster of RIB 40 was analyzed. Although most genes in the corresponding cluster exhibited from 97 to 99 % similarity to those of Aspergillus flavus, three genes shared 93 % similarity or less. In addition, although slight expression of aflR, positive transcriptional regulator gene, was detected in some A. oryzae strains having seven aflatoxin biosynthesis homologous genes, other genes related to aflatoxin production were not detected. RIB strains were mainly divided into group 1, having seven aflatoxin biosynthesis homologous genes (aflT, nor-i, aflR, norA, avnA, verB, and vbs), and group 2, having three homologous (avnA, verB, and vbs). Partial aflatoxin homologous gene cluster of RIB62 from group 2 was sequenced and compared with that of RIB40 from group 1. RIB62 showed a large deletion upstream of ver-1 with more than half of the aflatoxin homologous gene cluster missing including aflR, a positive transcriptional regulatory gene. Adjacent to the deletion of the aflatoxin homologous gene cluster, RIB62 has a unique sequence of about 8kb and a telomere. Southern analysis of A. oryzae RIB strains with four kinds of probe derived from the unique sequence of RIB62 showed that all group 2 strains have identical hybridizing signals. Polymerase chain reaction with specific primer set designed to amplify the junction between ver-1 and the unique sequence of RIB62 resulted in the same size of DNA fragment only from group 2 strains. Based on these results, we developed a useful genetic tool that distinguishes A. oryzae group 2 strains from the other groups' strains and propose that it might have differentiated from the ancestral strains due to chromosomal breakage.

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