Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Molecular detection of bat coronaviruses in three bat species in Indonesia

  • Dharmayanti, Ni Luh Putu Indi (Indonesian Research Center for Veterinary Science, Ministry of Agriculture) ;
  • Nurjanah, Diana (Indonesian Research Center for Veterinary Science, Ministry of Agriculture) ;
  • Nuradji, Harimurti (Indonesian Research Center for Veterinary Science, Ministry of Agriculture) ;
  • Maryanto, Ibnu (Research Centre for Biology, Indonesian Institute of Sciences (LIPI)) ;
  • Exploitasia, Indra (Biodiversity Conservation for the Indonesian Ministry of Environment and Forestry) ;
  • Indriani, Risa (Indonesian Research Center for Veterinary Science, Ministry of Agriculture)
  • Received : 2021.03.21
  • Accepted : 2021.07.16
  • Published : 2021.11.30
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Abstract

Bats are an important reservoir of several zoonotic diseases. However, the circulation of bat coronaviruses (BatCoV) in live animal markets in Indonesia has not been reported. Genetic characterization of BatCoV was performed by sequencing partial RdRp genes. Real-time polymerase chain reaction based on nucleocapsid protein (N) gene and Enzyme-linked immunosorbent assay against the N protein were conducted to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA and antibody, respectively. We identified the presence of BatCoV on Cynopterus brachyotis, Macroglossus minimus, and Rousettus amplexicaudatus. The results showed that the BatCoV included in this study are from an unclassified coronavirus group. Notably, SARS-CoV-2 viral RNA and antibodies were not detected in the sampled bats.

Keywords

Acknowledgement

The authors express their appreciation and thanks to Teguh S, Ace ES, Suraida Meisari DVM, Zaki Aminullah DVM for technical assistance and the agricultural services of the respective regencies/cities staff for field activity assistance. Ni Luh Putu Indi Dharmayanti and Diana Nurjanah are the main authors and contributed equally to this article.

References

  1. Voigt CC, Kingston T. Bats in the anthropocene. In: Bats in the Anthropocene: Conservation of Bats in a Changing World. Cham: Springer International Publishing; 2016, 1-9.
  2. O'Shea TJ, Cryan PM, Cunningham AA, Fooks AR, Hayman DT, Luis AD, et al. Bat flight and zoonotic viruses. Emerg Infect Dis. 2014;20(5):741-745. https://doi.org/10.3201/eid2005.130539
  3. Sendow I, Field H, Adjid RMA, Syafriati T, Darminto D, Morrissy C, et al. Seroepidemiologi Nipah virus pada kalong dan ternak babi di beberapa wilayah di Indonesia. J Biologi Indones. 2008;5(1):35-44.
  4. Sendow I, Field H, Ratnawati A, Adjid RMA, Saepulloh M, Breed A, et al. Status infeksi virus hendra pada kalong (Pteropus Spp.) Di Pontianak, Kalimantan Barat Dan Manado, Sulawesi Utara. J Biologi Indones. 2013;9(1):31-38.
  5. Anthony SJ, Johnson CK, Greig DJ, Kramer S, Che X, Wells H, et al. Global patterns in coronavirus diversity. Virus Evol. 2017;3(1):vex012. https://doi.org/10.1093/ve/vex012
  6. Maryanto I, Achmadi AS, Sulistyadi E, Wiantoro S, Maharadatunkamsi, Yoneda M, et al. Checklist of The Mammals of Indonesia: Scientific, English, Indonesia Name and Distribution Area Table in Indonesia Including CITES, IUCN and Indonesian Category for Conservation, Vol. 3. Bogor: Puslitbang Biologi LIPI; 2019.
  7. Suyanto A, Kartikasari SN. Kelelawar di Indonesia. Bogor: Puslitbang Biologi LIPI; 2001.
  8. Wong AC, Li X, Lau SK, Woo PC. Global epidemiology of bat coronaviruses. Viruses. 2019;11(2):174. https://doi.org/10.3390/v11020174
  9. Dinde AO, Mobio AJ, Konan AG, Fokou G, Yao K, Esso EL, et al. Response to the Ebola-related bushmeat consumption ban in rural Cote d'Ivoire. Agric Food Secur. 2017;6(1):28. https://doi.org/10.1186/s40066-017-0105-9
  10. Vats R, Thomas S. A study on use of animals as traditional medicine by Sukuma Tribe of Busega District in North-western Tanzania. J Ethnobiol Ethnomed. 2015;11(1):38. https://doi.org/10.1186/s13002-015-0001-y
  11. Subudhi S, Rapin N, Misra V. Immune system modulation and viral persistence in bats: understanding viral spillover. Viruses. 2019;11(2):192. https://doi.org/10.3390/v11020192
  12. Monagin C, Paccha B, Liang N, Trufan S, Zhou H, Wu D, et al. Serologic and behavioral risk survey of workers with wildlife contact in China. PLoS One. 2018;13(4):e0194647. https://doi.org/10.1371/journal.pone.0194647
  13. Woo PC, Lau SK, Li KS, Poon RW, Wong BH, Tsoi HW, et al. Molecular diversity of coronaviruses in bats. Virology. 2006;351(1):180-187. https://doi.org/10.1016/j.virol.2006.02.041
  14. Kurpiers LA, Schulte-Herbruggen B, Ejotre I, Reeder DM. Bushmeat and emerging infectious diseases: lessons from Africa. In: Angelici FM, editor. Problematic Wildlife: a Cross-Disciplinary Approach. Cham: Springer International Publishing; 2016, 507-551.
  15. Memish ZA, Cotten M, Meyer B, Watson SJ, Alsahafi AJ, Al Rabeeah AA, et al. Human infection with MERS coronavirus after exposure to infected camels, Saudi Arabia, 2013. Emerg Infect Dis. 2014;20(6):1012-1015. https://doi.org/10.3201/eid2006.140402
  16. Han HJ, Yu H, Yu XJ. Evidence for zoonotic origins of Middle East respiratory syndrome coronavirus. J Gen Virol. 2016;97(2):274-280. https://doi.org/10.1099/jgv.0.000342
  17. Wang LF, Eaton BT. Bats, civets and the emergence of SARS. Curr Top Microbiol Immunol. 2007;315:325-344.
  18. Chan JF, Kok KH, Zhu Z, Chu H, To KK, Yuan S, et al. Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan. Emerg Microbes Infect. 2020;9(1):221-236. https://doi.org/10.1080/22221751.2020.1719902
  19. Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020;579(7798):270-273. https://doi.org/10.1038/s41586-020-2012-7
  20. Ding Y, He L, Zhang Q, Huang Z, Che X, Hou J, et al. Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in SARS patients: implications for pathogenesis and virus transmission pathways. J Pathol. 2004;203(2):622-630. https://doi.org/10.1002/path.1560
  21. Chan JF, Yuan S, Kok KH, To KK, Chu H, Yang J, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet. 2020;395(10223):514-523. https://doi.org/10.1016/s0140-6736(20)30154-9
  22. Sheherazade , Tsang SM. Quantifying the bat bushmeat trade in North Sulawesi, Indonesia, with suggestions for conservation action. Glob Ecol Conserv. 2015;3:324-330. https://doi.org/10.1016/j.gecco.2015.01.003
  23. Wang LF, Shi Z, Zhang S, Field H, Daszak P, Eaton BT. Review of bats and SARS. Emerg Infect Dis. 2006;12(12):1834-1840. https://doi.org/10.3201/eid1212.060401
  24. Anindita PD, Sasaki M, Setiyono A, Handharyani E, Orba Y, Kobayashi S, et al. Detection of coronavirus genomes in Moluccan naked-backed fruit bats in Indonesia. Arch Virol. 2015;160(4):1113-1118. https://doi.org/10.1007/s00705-015-2342-1
  25. Febriani W, Saepuloh U, Ayuningsih E, Saputra R, Purbatrapsila A, Nangoy M, et al. Bat coronavirus of Pteropus alecto from Gorontalo Province, Indonesia. Int J Trop Vet Biomed Res. 2018;3(2):36-42. https://doi.org/10.21157/ijtvbr.v3i2.12359
  26. Morgan CN, Ammerman LK, Demere KD, Doty JB, Nakazawa YJ, Mauldin MR. Field identification key and guide for bats of the United States of America. Occas Pap Tex Tech Univ Mus. 2019;360:360.
  27. Woo PC, Lau SK, Lam CS, Lau CC, Tsang AK, Lau JH, et al. Discovery of seven novel mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus. J Virol. 2012;86(7):3995-4008. https://doi.org/10.1128/JVI.06540-11
  28. Yuliadi B, Sari TF, Handayani FD. Kelelawar Sulawesi Jenis dan Peranannya. Jakarta: Penerbit Badan Penelitian dan Pengembangan Kesehatan; 2018.
  29. van Boheemen S, de Graaf M, Lauber C, Bestebroer TM, Raj VS, Zaki AM, et al. Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans. mBio. 2012;3(6):e00473-12.
  30. Chomel BB, Belotto A, Meslin FX. Wildlife, exotic pets, and emerging zoonoses. Emerg Infect Dis. 2007;13(1):6-11. https://doi.org/10.3201/eid1301.060480
  31. Aguirre AA, Catherina R, Frye H, Shelley L. Illicit wildlife trade, wet markets, and COVID-19: preventing future pandemics. World Med Health Policy. 2020;12(3):256-265. https://doi.org/10.1002/wmh3.348