• CELLMED
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• v.2 no.3
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• pp.27.1-27.6
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• 2012

Red ginseng, which has a variety of biological and pharmacological activities including antioxidant, anti-inflammatory, antimutagenic and anticarcinogenic effects, has been used for thousands of years as a general tonic in traditional oriental medicine. Here, we tested the immune regulatory activities of hydrolyzed red ginseng by malted barley (HRG) on the expressions of receptor interacting proteins (Rip) 2 and $I{\kappa}B$ kinase-beta (IKK-${\beta}$) in mouse peritoneal macrophages. We show that HRG increased the activations of Rip 2 and IKK-${\beta}$ for the first time. When HRG was used in combination with recombinant interferon-${\gamma}$ (rIFN-${\gamma}$), there was a marked cooperative induction of nitric oxide (NO) production. The increased expression of inducible NO synthase from rIFN-${\gamma}$ plus HRG-stimulated cells was almost completely inhibited by pre-treatment with pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor-${\kappa}B$ (NF-${\kappa}B$). In addition, the treatment of peritoneal macrophages with rIFN-${\gamma}$ plus HRG caused significant increases in tumor necrosis factor (TNF)-${\alpha}$ mRNA expression and production. Because NO and TNF-${\alpha}$ play an important role in the immune function and host defense, HRG treatment can modulate several aspects of the host defense mechanisms as a result of the stimulations of the inducible nitric oxide synthase and NF-${\kappa}B$. In conclusion, our findings demonstrate that HRG increases the productions of NO and TNF-${\alpha}$ from rIFN-${\gamma}$-primed macrophages and suggest that Rip2/IKK-${\beta}$ plays a critical role in mediating these immune regulatory effects of HRG.

• Applied Biological Chemistry
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• v.38 no.6
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• pp.528-533
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• 1995

An antiviral protein (AAP29) with ribosome-inactivating activity was purified and characterized from the leaves of the Amaranthus mangostanus. Purification was accomplished through crude extraction, ammonium sulfate precipitation, S-Sepharose chromatography, gel filtration, CM-Sepharose chromatography and Blue sepharose chromatography. This protein was about 29.2 kDa and strongly basic with the PI value between 9.0 and 9.6, indicating that AAP29 is similar to Type 1 RIP. The AAP29 showed high thermostability without activity toss even after 20 min at $50^{\circ}C$. In cell free system using rabbit reticulocyte lysate, AAP29 inhibited protein synthesis with an $IC_{50}$, of 0.18 nM. This protein also reduced mosaic symptoms of cucumber mosaic virus (CMV) on tobacco leaves. The N-terminal amino acid sequences of the AAP29 are ADLTFTVTKDGTSQSYXTLXNXWRXW and shows no sequence similarity with any known RIPs.

• Bulletin of the Society of Naval Architects of Korea
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• v.52 no.1
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• pp.22-30
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• 2015

• Journal of the Korean Society of Clothing and Textiles
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• v.2 no.1
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• pp.177-183
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• 1978

Byul-Gam is a low-ranking official who takes charge of sundry services in Yi-Dynasty court, and escorts his king when he make a honored going. Uniforms of Byul-Gam are noted for their gaiety. This study aims at inquiring into uniforms of Byul-Gam through historical records. The resultant findings are as follows: 1) Uniforms of Byul-Gam are divided into full dress and ordinary clothes. 2) The full dress has two kinds of style. One is Dan-Ryung, a kind of robe, clad together with Gun as a headdress. This robe is blue, Gun for king's Byul-Gam violet, and Gun for the Crown Prince's Byul-Gam blue. The other is a combination of yellow Cho-Rip and red Chup-Ri. This is a military uniform. 3) Ordinary dresses for Byul-Gam are orange Cho-Rip and red Jik-Ryung. Underwear in this case is Hyup-Joo-Um and Chup-Ri. Hyup-Joo-Um is a kind of robe. Inside it Korean style of jacket and slacks are usually clad. 4) As time passed, the uniform of Byul-Gam had been changed from Cheong-Dan-Ryung to Hong-Dan-Ryung, and Jik-Ryung had been shifted to a full dress. 5) Only five remains of Jik-Ryung for Byul-Gam have been handed down to us. The analysis of those indicate that they gradually became similar to Duru-Magi, a kind of Korean robe. This fact is common in the other transfiguring process of dresses of robe kind.

• Journal of the Korean Graphic Arts Communication Society
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• v.25 no.1
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• pp.81-94
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• 2007

Recently, the trend in the printing industry includes shorter run lengths and with fast turnaround times. As new markets have made it possible to produce small quantities of high-quality color products at affordable price, the general commercial printing meets the customer's diverse demand by using spot color besides process four colors. Especially, by using spot color for printing the enterprise's logo or specific color, we can see the effect of printing is getting better. With the combination of the right software, ink, media, and device can be treated as a digital proofer for spot color printing, providing significant time and cost savings compared to conventional procedures. The objective of this study is to investigate the quality of spot color proofs printed by ink-jet and dye sublimation proofer using ICC-based color management system. An Epson Stylus Color 3000 ink-jet proofer combined with Best Color Proof XXL RIP was tested for glossy and matte paper. 3M Rainbow dye sublimation proofer was examined using 3M Rainbow controller ver. 4.1 RIP on the manufacturer recommended proofing paper. ICC profiles were generated for each device using ECI 2002 visual target and evaluated for the accuracy of process 4 color reproduction. The test charts consisting of Pantone color 1140 was selected to test the quality of spot color reproduction.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.137-137
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• 2019

돌출줄눈은 산지하천의 만곡부의 빠른 유속을 감소시키기 위하여 활용된다. 본 연구에서는 친환경 디자인의 나무줄기 돌출줄눈(Tree Trunk Rip, TTR)과 사다리꼴 돌출줄눈(Trapezoid Rip, TR)의 흐름저항을 비교 분석하기 위하여 개수로 수리실험을 수행하였다. 실험은 길이 9m, 폭이 0.6m이며 경사가 0.0035로 고정된 개수로의 한쪽 측벽에 돌출줄눈을 설치하여 진행하였다. 사다리 꼴 돌출줄눈의 형상은 밑변 각이 $63^{\circ}$이며 무차원 설치간격 ${\lambda}_{nv}$가 6, 9, 12인 경우이다. 나무줄기 돌출줄눈의 기본 형상은 사다리꼴이고 표면은 나무껍질 무늬이며 ${\lambda}_{nv}$가 약 10이다. 나무줄기 돌출줄눈의 간격은 사다리꼴 돌출줄눈의 최적 설치 간격 9~12배 범위에 해당되고 평균 마찰계수는 사다리꼴 돌출줄눈의 9~12배의 평균 마찰계수 범위에 포함되었다. 사다리꼴 돌출줄눈의 ${\lambda}_{nv}$가 9, 12일 때의 전체 저항에 대한 형상저항의 비는 평균 $69.4{\pm}5.8%$였으며 나무줄기 돌출줄눈은 $70.2{\pm}2.1%$로 사다리꼴 돌출줄눈과 유사하다. 산지하천 흐름저항을 위한 돌출줄눈 설치에 있어 친환경적 디자인을 고려한 나무줄기가 사다리꼴 돌출줄눈보다 활용도가 클 것으로 기대된다.

• Analytical Science and Technology
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• v.19 no.3
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• pp.218-225
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• 2006

A molecularly imprinted polymer (MIP) was synthesized by radiation-induced polymerization (RIP), where the ferulic acid was used as a template molecule, 4-vinylpyridine as a monomer and ethylene glycoldimethacrylate (EGDMA) as a cross-linking monomer. The MIP was packed in a glass column using a slurry method for use in medium pressure liquid chromatography (MPLC). The MPLC column was tested for separation and purification of ferulic acid from the rice oil. When repeated three times, the MPLC separation/purification yielded the ferulic acid with the purity higher than ~99%. The chemiluminescence of the luminal (5-amino-2,3-dihydro-1,4-phtalazinedione) measured on a potato disc slide (5.0 mm thick) was enhanced in the presence of ferulic acid, while, without the ferulic acid, the chemiluminescence of luminol on the potato slice disc was not observed, which suggests the ferulic acid obtained from the rice oil can be useful for immunoassay.

• Molecules and Cells
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• v.45 no.4
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• pp.257-272
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• 2022

In addition to inducing apoptosis, caspase inhibition contributes to necroptosis and/or autophagy depending on the cell type and cellular context. In macrophages, necroptosis can be induced by co-treatment with Toll-like receptor (TLR) ligands (lipopolysaccharide [LPS] for TLR4 and polyinosinic-polycytidylic acid [poly I:C] for TLR3) and a cell-permeable pan-caspase inhibitor zVAD. Here, we elucidated the signaling pathways and molecular mechanisms of cell death. We showed that LPS/zVAD- and poly I:C/zVAD-induced cell death in bone marrow-derived macrophages (BMDMs) was inhibited by receptor-interacting protein kinase 1 (RIP1) inhibitor necrostatin-1 and autophagy inhibitor 3-methyladenine. Electron microscopic images displayed autophagosome/autolysosomes, and immunoblotting data revealed increased LC3II expression. Although zVAD did not affect LPS- or poly I:C-induced activation of IKK, JNK, and p38, it enhanced IRF3 and STAT1 activation as well as type I interferon (IFN) expression. In addition, zVAD inhibited ERK and Akt phosphorylation induced by LPS and poly I:C. Of note, zVAD-induced enhancement of the IRF3/IFN/STAT1 axis was abolished by necrostatin-1, while zVAD-induced inhibition of ERK and Akt was not. Our data further support the involvement of autocrine IFNs action in reactive oxygen species (ROS)-dependent necroptosis, LPS/zVAD-elicited ROS production was inhibited by necrostatin-1, neutralizing antibody of IFN receptor (IFNR) and JAK inhibitor AZD1480. Accordingly, both cell death and ROS production induced by TLR ligands plus zVAD were abrogated in STAT1 knockout macrophages. We conclude that enhanced TRIF-RIP1-dependent autocrine action of IFNβ, rather than inhibition of ERK or Akt, is involved in TLRs/zVAD-induced autophagic and necroptotic cell death via the JAK/STAT1/ROS pathway.

• Journal of Korean Society for Quality Management
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• v.21 no.1
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• pp.78-95
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• 1993

The art of designing and analyzing fault-tolerant computers is surveyed with special emphasis on problems of analyzing the behavior of computers that have autonomous repair capability. The survey covers the following topics : (1) general issues in computer reliability, (2) fault-tolerance state relations and requirements, (3) computational hierarchy, (4) fault characteristics, (5) fault diagnosis, (6) fault-tolerance schemes for logic network and machines, (7) fault-coverage effects, and (8) fault-tree analysis of coverage. This paper does not include techniques for verifying nonredundant hardware or system software designs or for verifying the correctness of application programs.

• CELLMED
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• v.2 no.3
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• pp.29.1-29.9
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• 2012

The prevalence of allergic disease has been increasing over the past few decades in the majority of Western industrialized nations. There are some socioeconomic disparities regarding allergic disease status and management. Pyeongwee-San (KMP6) is Korean medicine for the treatment of gastrointestinal tract disease. It is known that KMP6 has an improving effect on the spleen and stomach functions in traditional Korean medical theory. Here, we hypothesized that KMP6 could be used to regulate the inflammatory reaction. We show the molecular mechanisms of Pyeongwee-San (KMP6) on inflammatory reactions. A molecular docking simulation showed that hesperidin, component of KMP6, regulate the enzymatic activity by interaction in the active site of caspase-1. KMP6 control the activity of caspase-1 in activated human mast cell line (HMC-1 cells). KMP6 reduced the expression of receptor interacting protein (RIP)-2 in HMC-1 cells. Thymic stromal lymphopoietin protein production and mRNA expression were inhibited by KMP6. In the activated HMC-1 cells, KMP6 suppressed the activation of mitogen-ativated protein kinase and nuclear factor-kappaB. In addition, KMP6 significantly inhibited the expression of inflammatory cytokines. Our findings indicate that KMP6 may attenuate allergic reactions via the regulation of caspase-1/RIP-2 signaling pathway. These studies will help advance the social welfare system.