• Korean Journal of Veterinary Research
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• v.31 no.2
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• pp.223-228
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• 1991

Most progesterone enzyme immunoassays(EIA) are used liquid-phase double-antibody or single-antibody seperation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants. Because of these several problems, we were prompted to develop an effective enzyme-linked immunosorbent assay(ELISA) system that would be equal or superior to RIA for assay of progesterone. The results were obtained as follows. 1. Cross reaction of the progesterone antiserum with other steroids determined was shown with progesterone(100%), $11{\alpha}$-deoxycorti-costerone(2.271%), but the other steroids were shown below 0.9%. 2. Standard curve for progesterone ELISA was shown available difference according to progesterone concentration from 0 to 1,000pg/ml. 3. The lower limit of sensitivity was 0.2pg/well 4. Progesterone concentration was 1.6ng/ml for before parturition, and that was below 0.5ng/ml for after parturition. This development enzyme-linked immunosorbent assay for progesterone can be detected pregnancy diagnosis in cattle, and also applicable 10 research on physiological function including such as reproductive disorders.

• Journal of The Korean Society of Integrative Medicine
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• v.6 no.1
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• pp.63-74
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• 2018

Purpose: The purpose of this study was to evaluate the effect of a trunk stabilization exercise program on the participants' scoliosis angle, pulmonary function, and growth hormones. Method: In the study, 30 participants were divided into a normal scoliosis exercise group (n=15) and an obese scoliosis exercise group (n=15). The participants performed a trunk stabilization exercise program three times a week for 12 weeks, and the exercise sessions lasted 50 minutes. The participants' pulmonary function [FVC, FEV1, FEV1/FVC, and PEF] was measured using a CardioTouch 3000S, and their scoliosis angles were measured using the Cobb's angle. The levels of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) were analyzed on an immunoradiometric assay (IRMA) and radioimmunoassay (RIA), respectively. Results: After the intervention, the scoliosis angle, hormone levels, and pulmonary function increased significantly in both groups (p<.05). The result of the intergroup difference test indicated statistically significant differences in the three items (scoliosis angle, hormone levels, and pulmonary function) between the two groups (p<.05). Conclusion: Therefore, this program may be recommended as a therapeutic intervention for patients with scoliosis.

• Journal of Embryo Transfer
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• v.7 no.2
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• pp.117-124
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• 1992

미성숙 래트의 외경정맥에 카테타를 장착하고, 다음날 (28일령) 대조군에는 4IU, 다배란 처치 군에는 20IU의 PMSG를 피하주사하였다. 각 실험동물은 혈중의 LH농도 변화를 측정하기 위하여 PMSG 투여 직전 (0시간), 투여후 12시간, 그 이후 6시간 간격으로 혈액을 채취하고 72시간에 희생시켰다. 그 결과 다배란 용량의 PMSG 투여는 먼저 배란반응 및 난소중량을 대조군에 비하여 각각 4,7배 및 2.1배나 현저하게 (P<0.05) 증가시켰다. 그리고 혈청 LH농도는 Radioimmunoassay(RIA)에 의하여 결정되었는데, 먼저 두 군 모두 두 개의 분명한 peak을 가진 경시적 변화관계를 보였다. 즉 이들 두 군네 있어서 LH농도 변화는 0-18시간대에 처음으로 완만한 증가와 54-60시간대에 두번째는 급격한 증가(surge)를 보였다. 그러나 두 군간에 LH농도의 크기는 현저하게 달라, 다배란처치군의 동물에 있어서는 두번째의 LH peak에 앞서 전반적인 LH농도가 대조군보다 현저하게 (P<0.001) 높았으며, 반대로 PMSG 투여후 60시간에 일어나는 peak에 있어서는 LH농도가 대조군보다 현저하게 (P<0.001) 54%나 낮았다. 덧붙여 두 peak간의 증가폭은 대조근에 비하여 다배란 처치군에서 훨씬 낮았다. 본 열구 결과는 PMSG 처치된 래트에 있어서 두 가지의 분명한LH peak의 존재를 정의하며, 다배란 처치에 따른 난소과잉 자극과 내인성 LH surge의 감소와의 연관성을 밝힌다.

• Nuclear Engineering and Technology
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• v.12 no.2
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• pp.121-126
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• 1980

Selection methods of well labelled insulin fractions based on two different criteria were compared to establish an efficient low level RIA of insulin and to elucidate the correlation between the immunoreactivity and the charcoal-adsorptivity of the radioiodine labelled insulin. The results indicated that the selection of well labelled insulin fractions by means of a charcoal-adsorption test is inappropriate. Generally, the distribution of radioactivity antibody-bindability, and charcoal-adsorptivity of the labelled insulin was not consistent with each other. Thus. the selection should be carried out for every labelling batch to get the utmost assay reliability by antibody-bindability but not by charcoal-adsorptivity. By using the well selected labelled insulin fractions based on antibody-binding, a correct assay for a reference serum was possible, and by extending the incubation time upto 96 hrs, a sharp dose response curve could be obtained even in the range of below 5 $\mu$U/ml standard insulin doses.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.19 no.5
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• pp.589-595
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• 2010

This paper presents a reliability-based shape optimization (RBSO) using the evolutionary structural optimization (ESO). An actual design involves uncertain conditions such as material property, operational load, poisson's ratio and dimensional variation. The deterministic optimization (DO) is obtained without considering of uncertainties related to the uncertainty parameters. However, the RBSO can consider the uncertainty variables because it has the probabilistic constraints. In order to determine whether the probabilistic constraint is satisfied or not, simulation techniques and approximation methods are developed. In this paper, the reliability-based shape design optimization method is proposed by utilization the reliability index approach (RIA), performance measure approach (PMA), single-loop single-vector (SLSV), adaptive-loop (ADL) are adopted to evaluate the probabilistic constraint. In order to apply the ESO method to the RBSO, a sensitivity number is defined as the change of strain energy in the displacement constraint. Numerical examples are presented to compare the DO with the RBSO. The results of design example show that the RBSO model is more reliable than deterministic optimization.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.117-117
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• 1997

Sandimmune Neoral$\^$(R)/ and Neoplanta$\^$(R)/ capsules were administered to twenty four healthy Korean male subjects at a cyclosporin A (CsA) dose of 175 mg in a 2 ${\times}$ 2 crossover investigation with a two-week wash-out phase. Concentrations of CsA in blood were measured by RIA method for over a period of 48 h. Result : The two formulations were found bioequivalent, but analysis of variance (ANOVA) indicated that there is a significant (p<0.01) period effect in AUC$\_$0-LAST/ (area under the blood concentration above assay limit of quantification-time curve) and C$\_$MAX/ (maximum blood concentration) between the administrations. Paired t-test revealed 6 and 9% decreases in AUC$\_$0-LAST/ and C$\_$MAX/, respectively at the second administration. This period effect on the pharmacokinetics of CsA may be relevant for the patients who need consecutive administration of the drug. A number of mechanisms, such as induction of the enzymes responsible for metabolism of the drug in the gut wall and/or liver and modulation of P-glycoprotein upon the consecutive dosing, appear consistent with the change, and needs experimental proof.

• Fisheries and Aquatic Sciences
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• v.2 no.2
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• pp.176-181
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• 1999

The variations of gene expression and pituitary contents of GTH subunits during the ovarian development of masu salmon, Oncorhynchus masou, were investigated. The pituitary GTHs contents was measured by radioimmunoassays (RIAs) using purified GTH subunits and their antibodies. Pituitary contents of GTH $I\beta$ gradually increased from April through August, and reached the maximum in October. On the other hand, pituitary contents of GTH $II\beta$ remained low until August, but they rapidly increased in October. Total RNAs were prepared from pooled pituitaries and the GTH subunits mRNA in pituitaries was quantified by Northern blot hybridization using masu salmon cDNA probes for each GTH subunit. GTH $I\beta$ mRNA level increased with the progression of ovarian maturity. However, GTH $II\beta$ mRNA was detected only at a more advanced stage, and were extremly high at ovulation. A high levels for GTH a mRNA was detected only at ovulation stage. The synchronous increase in pituitary contents and mRNA levels suggested that ovarian maturity in masu salmon was regulated by both GTH I and GTH II.

• Nutritional Sciences
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• v.8 no.3
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• pp.196-199
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• 2005

A study during second trimester of pregnancy was conducted among 100 women from a population with mild to moderate deficient iodine intake (median 7 ${\mu}g$/L)to monitor the changes in serum free thyroxin ($FT_4$), triiodothyionine ($T_3$) and thyrtropin (TSH). Thyroid related hormones were assessed using radioimmunoassay (RIA) and immunoradiometricassay (IRMA). As compared with non pregnant women, mean $FT_4$ levels decreased, $T_3$ levels increased and TSH levels were slightly depressed in pregnant women. Mean $FT_4$ concentration reached its highest level in the $4^{th}$ month and linearly decreased until the $6^{th}$ month. Mean $T_3$ level remained almost constant during $2^{nd}$ trimester of pregnancy. Mean TSH level remained unaltered during the $4^{th}$ and $5^{th}$ months and was slightly lower than that for the control group. However, these differences were statistically insignificant At the end of the second trimester, hypothyroxinemia and $T_3$ levels greater than the upper limit of the laboratory reference range were found in $34\%$ and $38\%$ of women, respectively. These changes in thyroid hormones reflect typical adaptations of the thyroid gland during pregnancy to the limited availability of iodine.

• Advances in Traditional Medicine
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• v.7 no.2
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• pp.197-204
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• 2007

The present study focused on the establishment of micropropagation protocol for the high value Pluchea (P.) indica (L.) Less., genotype, an important medicinal plant and evaluation of the diuretic activity of the leaf extract of the tissue cultured plant. Leaf explants, nodal segments and shoot tips were cultured in MS medium supplemented with auxin and cytokinin and their combinations. With the objective of inducing callus giving rise to new adult plants, naphthalene acetic acid was found to be most effective for (80%) for callus induction. The methanolic extract of leaves of the micropropagated P. indica was investigated for its diuretic activity in Wistar albino rats. Urinary excretion parameters were studied for evaluation of diuretic activity using Frusemide (20 mg/kg, p.o.) as standard. The extract showed significant diuretic activity at the doses of 100, 200 and 300 mg/kg. p.o. An oral acute toxicity study for the extract was carried out and the $LD_{50}$ value was found to be 2,825 mg/kg body weight.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.17 no.6
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• pp.77-82
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• 2008

This paper presents a Reliability-Based Topology Optimization(RBTO) using the Evolutionary Structural Optimization(ESO). An actual design involves some uncertain conditions such as material property, operational load and dimensional variation. The Deterministic Topology Optimization(DTO) is obtained without considering the uncertainties related to the uncertainty parameters. However, the RBTO can consider the uncertainty variables because it has the probabilistic constraints. In order to determine whether the probabilistic constraints are satisfied or not, simulation techniques and approximation methods are developed. In this paper, the reliability index approach(RIA) is adopted to evaluate the probabilistic constraints. In order to apply the ESO method to the RBTO, sensitivity number is defined as the change in the reliability index due to the removal of the ith element. Numerical examples are presented to compare the DTO with the RBTO.